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Protein Page:
Akt2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Akt2 an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family
Chromosomal Location of Human Ortholog: 19q13.1-q13.2
Cellular Component: plasma membrane; cell cortex; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; kinase activity; ATP binding
Biological Process: fat cell differentiation; glycogen biosynthetic process; apoptosis; positive regulation of glycogen biosynthetic process; carbohydrate transport; myelin maintenance in the peripheral nervous system; intracellular protein transport across a membrane; positive regulation of vesicle fusion; protein modification process; glucose metabolic process; signal transduction; protein amino acid phosphorylation; regulation of cell migration; positive regulation of fatty acid beta-oxidation; regulation of translation; positive regulation of glucose import; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; activation of Ral GTPase
Reference #:  P31751 (UniProtKB)
Alt. Names/Synonyms: AKT2; Murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-BETA; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
Gene Symbols: AKT2
Molecular weight: 55,769 Da
Basal Isoelectric point: 5.98  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Akt2

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
enzymatic activity, induced: T309‑p, S474‑p
intracellular localization: T309‑p, S474‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K30-ub RPRYFLLkSDGsFIG
0 3 S34-p FLLkSDGsFIGyKER
0 1 Y38-p SDGsFIGyKERPEAP
0 1 T81-p VIRCLQWtTVIERTF
0 2 Y122-p PGEDPMDyKCGsPsD
2 8 S126-p PMDyKCGsPsDSsTT
1 0 S128-p DyKCGsPsDSsTTEE
0 1 S130 KCGsPsDSsTTEEME
1 0 S131-p CGsPsDSsTTEEMEV
0 1 S141-p EEMEVAVsKARAKVT
0 1 Y177-p REKATGRyyAMKILR
0 1 Y178-p EKATGRyyAMKILRK
0 1 S242-p GELFFHLsRERVFTE
0 1 Y255 TEERARFYGAEIVSA
0 1 S261 FYGAEIVSALEYLHS
0 1 Y265 EIVSALEYLHSRDVV
0 1 K277-ub DVVYRDIkLENLMLD
0 3 S302-p GLCKEGIsDGAtMKt
0 8 T306-p EGIsDGAtMKtFCGt
9 19 T309-p sDGAtMKtFCGtPEy
0 4 T313-p tMKtFCGtPEyLAPE
0 12 Y316-p tFCGtPEyLAPEVLE
0 27 Y327-p EVLEDNDyGRAVDWW
0 1 K378-ac RTLSPEAksLLAGLL
0 1 K378-ub RTLSPEAksLLAGLL
0 2 S379-p TLSPEAksLLAGLLK
0 4 Y438-p TSEVDTRyFDDEFTA
0 2 S447-p DDEFTAQsItItPPD
0 1 T449-p EFTAQsItItPPDRy
1 19 T451-p TAQsItItPPDRyDs
0 2 Y456-p tItPPDRyDsLGLLE
0 4 S458-p tPPDRyDsLGLLELD
0 1 L461 DRyDsLGLLELDQRT
16 52 S474-p RTHFPQFsysAsIRE
0 22 Y475-p THFPQFsysAsIRE_
0 9 S476-p HFPQFsysAsIRE__
0 49 S478-p PQFsysAsIRE____
13038 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb
13842 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb (PE Conjugate)
9267 : Phospho-Akt (Thr450) Antibody
12178 : Phospho-Akt (Thr450) (D5G4) Rabbit mAb
2336 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2337 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
3787 : Phospho-Akt (Ser473) (736E11) Rabbit mAb
4051 : Phospho-Akt (Ser473) (587F11) Mouse mAb
4058 : Phospho-Akt (Ser473) (193H12) Rabbit mAb
4060 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb
4070 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Sepharose Bead Conjugate)
4071 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4075 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5012 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Biotinylated)
5102 : Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
5315 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (PE Conjugate)
8599 : Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific)
9271 : Phospho-Akt (Ser473) Antibody
9563 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Magnetic Bead Conjugate)
11962 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (APC Conjugate)
12694 : Phospho-Akt (Ser473) (D9W9U) Mouse mAb
  mouse

 
K30 RPRYFLLKSDGSFIG
S34 FLLKSDGSFIGYKER
Y38 SDGSFIGYKERPEAP
T81 VIRCLQWTTVIERTF
Y122 PGEDAMDYKCGsPSD
S126-p AMDYKCGsPSDsSTS
S128 DYKCGsPSDsSTSEM
S130-p KCGsPSDsSTSEMME
S131 CGsPSDsSTSEMMEV
N141 EMMEVAVNKARAKVT
Y177 REKATGRYYAMKILR
Y178 EKATGRYYAMKILRK
S242 GELFFHLSRERVFTE
Y255-p TEDRARFyGAEIVsA
S261-p FyGAEIVsALEyLHS
Y265-p EIVsALEyLHSRDVV
K277 DVVYRDIKLENLMLD
S302 GLCKEGISDGAtMKt
T306-p EGISDGAtMKtFCGt
T309-p SDGAtMKtFCGtPEY
T313-p tMKtFCGtPEYLAPE
Y316 tFCGtPEYLAPEVLE
Y327-p EVLEDNDyGRAVDWW
K378 RTLGPEAKSLLAGLL
K378 RTLGPEAKSLLAGLL
S379 TLGPEAKSLLAGLLK
Y438 TSEVDTRYFDDEFTA
S447-p DDEFTAQsITItPPD
T449 EFTAQsITItPPDRy
T451-p TAQsITItPPDRyDs
Y456-p TItPPDRyDsLDPLE
S458-p tPPDRyDsLDPLELD
P461 DRyDsLDPLELDQRT
S474-p RTHFPQFsysAsIRE
Y475-p THFPQFsysAsIRE_
S476-p HFPQFsysAsIRE__
S478-p PQFsysAsIRE____
13038 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb
13842 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb (PE Conjugate)
9267 : Phospho-Akt (Thr450) Antibody
12178 : Phospho-Akt (Thr450) (D5G4) Rabbit mAb
2336 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2337 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
3787 : Phospho-Akt (Ser473) (736E11) Rabbit mAb
4051 : Phospho-Akt (Ser473) (587F11) Mouse mAb
4058 : Phospho-Akt (Ser473) (193H12) Rabbit mAb
4060 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb
4070 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Sepharose Bead Conjugate)
4071 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4075 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5012 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Biotinylated)
5102 : Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
5315 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (PE Conjugate)
8599 : Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific)
9271 : Phospho-Akt (Ser473) Antibody
9563 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Magnetic Bead Conjugate)
11962 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (APC Conjugate)
12694 : Phospho-Akt (Ser473) (D9W9U) Mouse mAb
  rat

 
K30 RPRYFLLKSDGSFIG
S34 FLLKSDGSFIGYKER
Y38 SDGSFIGYKERPEAP
T81 VIRCLQWTTVIERTF
Y122 PGEDAMDYKCGSPSD
S126 AMDYKCGSPSDSSTS
S128 DYKCGSPSDSSTSEM
S130 KCGSPSDSSTSEMME
S131 CGSPSDSSTSEMMEV
S141 EMMEVAVSKARAKVT
Y177 REKATGRYYAMKILR
Y178 EKATGRYYAMKILRK
S242 GDLFFHLSRERVFTE
Y255 TEDRARFYGAEIVSA
S261 FYGAEIVSALEYLHS
Y265 EIVSALEYLHSTDVV
K277 DVVYRDIKLENLMLD
S302 GLSKEGISDGATMKt
T306 EGISDGATMKtFCGT
T309-p SDGATMKtFCGTPEY
T313 TMKtFCGTPEYLAPE
Y316 tFCGTPEYLAPEVLE
Y327 EVLEDNDYGRAVDWW
K378 RTLGPEAKSLLAGLL
K378 RTLGPEAKSLLAGLL
S379 TLGPEAKSLLAGLLK
Y438 TSEVDTRYFDDEFTA
S447 DDEFTAQSITItPPD
T449 EFTAQSITItPPDRY
T451-p TAQSITItPPDRYDS
Y456 TItPPDRYDSLGsLE
S458 tPPDRYDSLGsLELD
S461-p DRYDSLGsLELDQRT
S474-p RTHFPQFsYSASIRE
Y475 THFPQFsYSASIRE_
S476 HFPQFsYSASIRE__
S478 PQFsYSASIRE____
13038 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb
13842 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb (PE Conjugate)
9267 : Phospho-Akt (Thr450) Antibody
12178 : Phospho-Akt (Thr450) (D5G4) Rabbit mAb
2336 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2337 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
3787 : Phospho-Akt (Ser473) (736E11) Rabbit mAb
4051 : Phospho-Akt (Ser473) (587F11) Mouse mAb
4058 : Phospho-Akt (Ser473) (193H12) Rabbit mAb
4060 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb
4070 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Sepharose Bead Conjugate)
4071 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4075 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5012 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Biotinylated)
5102 : Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
5315 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (PE Conjugate)
8599 : Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific)
9271 : Phospho-Akt (Ser473) Antibody
9563 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Magnetic Bead Conjugate)
11962 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (APC Conjugate)
12694 : Phospho-Akt (Ser473) (D9W9U) Mouse mAb
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