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Protein Page:
Akt2 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Akt2 an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family
Cellular Component: plasma membrane; cell cortex; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; kinase activity; ATP binding
Biological Process: fat cell differentiation; glycogen biosynthetic process; apoptosis; positive regulation of glycogen biosynthetic process; carbohydrate transport; intracellular protein transport across a membrane; myelin maintenance in the peripheral nervous system; positive regulation of vesicle fusion; protein modification process; glucose metabolic process; signal transduction; regulation of cell migration; positive regulation of fatty acid beta-oxidation; regulation of translation; positive regulation of glucose import; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; activation of Ral GTPase
Reference #:  P31751 (UniProtKB)
Alt. Names/Synonyms: AKT2; Murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-BETA; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
Gene Symbols: AKT2
Molecular weight: 55,769 Da
Basal Isoelectric point: 5.98  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Alzheimer's Disease  |  AMPK Signaling  |  Angiogenesis  |  Apoptosis Regulation  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  G1/S Checkpoint  |  IL6 Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Microtubule Dynamics  |  Mitochondrial Control of Apoptosis  |  mTOR Signaling  |  NF-kB Signaling  |  PI3K/Akt Signaling  |  Protein Kinase C Signaling  |  T Cell Receptor Signaling  |  TGF-├č Signaling  |  Translation: eIF4E and p70S6K  |  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Akt2

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
enzymatic activity, induced: T309‑p, S474‑p
intracellular localization: T309‑p, S474‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K30-u RPRYFLLkSDGsFIG
0 1 S34-p FLLkSDGsFIGYKER
1 6 S126-p PMDYKCGsPSDSSTT
0 1 S130 KCGsPSDSSTTEEME
0 1 K277-u DVVYRDIkLENLMLD
0 3 S302-p GLCKEGIsDGAtMKt
0 7 T306-p EGIsDGAtMKtFCGt
9 14 T309-p sDGAtMKtFCGtPEy
0 4 T313-p tMKtFCGtPEyLAPE
0 12 Y316-p tFCGtPEyLAPEVLE
0 26 Y327-p EVLEDNDyGRAVDWW
0 1 K378-u RTLSPEAkSLLAGLL
0 3 Y438-p TSEVDTRyFDDEFTA
0 2 S447-p DDEFTAQsItItPPD
0 1 T449-p EFTAQsItItPPDRy
1 17 T451-p TAQsItItPPDRyDs
0 2 Y456-p tItPPDRyDsLGLLE
0 3 S458-p tPPDRyDsLGLLELD
16 46 S474-p RTHFPQFsysAsIRE
0 18 Y475-p THFPQFsysAsIRE_
0 8 S476-p HFPQFsysAsIRE__
0 44 S478-p PQFsysAsIRE____
13038 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb
9267 : Phospho-Akt (Thr450) Antibody
12178 : Phospho-Akt (Thr450) (D5G4) Rabbit mAb
2336 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2337 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
3787 : Phospho-Akt (Ser473) (736E11) Rabbit mAb
4051 : Phospho-Akt (Ser473) (587F11) Mouse mAb
4058 : Phospho-Akt (Ser473) (193H12) Rabbit mAb
4060 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb
4070 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Sepharose Bead Conjugate)
4071 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4075 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5012 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Biotinylated)
5102 : Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
5315 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (PE Conjugate)
8599 : Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific)
9271 : Phospho-Akt (Ser473) Antibody
9563 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Magnetic Bead Conjugate)
12694 : Phospho-Akt (Ser473) (D9W9U) Mouse mAb
  mouse

 
K30 RPRYFLLKSDGSFIG
S34 FLLKSDGSFIGYKER
S126-p AMDYKCGsPSDsSTS
S130-p KCGsPSDsSTSEMME
K277 DVVYRDIKLENLMLD
S302 GLCKEGISDGAtMKt
T306-p EGISDGAtMKtFCGt
T309-p SDGAtMKtFCGtPEY
T313-p tMKtFCGtPEYLAPE
Y316 tFCGtPEYLAPEVLE
Y327-p EVLEDNDyGRAVDWW
K378 RTLGPEAKSLLAGLL
Y438 TSEVDTRYFDDEFTA
S447-p DDEFTAQsITItPPD
T449 EFTAQsITItPPDRy
T451-p TAQsITItPPDRyDs
Y456-p TItPPDRyDsLDPLE
S458-p tPPDRyDsLDPLELD
S474-p RTHFPQFsysAsIRE
Y475-p THFPQFsysAsIRE_
S476-p HFPQFsysAsIRE__
S478-p PQFsysAsIRE____
13038 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb
9267 : Phospho-Akt (Thr450) Antibody
12178 : Phospho-Akt (Thr450) (D5G4) Rabbit mAb
2336 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2337 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
3787 : Phospho-Akt (Ser473) (736E11) Rabbit mAb
4051 : Phospho-Akt (Ser473) (587F11) Mouse mAb
4058 : Phospho-Akt (Ser473) (193H12) Rabbit mAb
4060 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb
4070 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Sepharose Bead Conjugate)
4071 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4075 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5012 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Biotinylated)
5102 : Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
5315 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (PE Conjugate)
8599 : Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific)
9271 : Phospho-Akt (Ser473) Antibody
9563 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Magnetic Bead Conjugate)
12694 : Phospho-Akt (Ser473) (D9W9U) Mouse mAb
  rat

 
K30 RPRYFLLKSDGSFIG
S34 FLLKSDGSFIGYKER
S126 AMDYKCGSPSDSSTS
S130 KCGSPSDSSTSEMME
K277 DVVYRDIKLENLMLD
S302 GLSKEGISDGATMKt
T306 EGISDGATMKtFCGT
T309-p SDGATMKtFCGTPEY
T313 TMKtFCGTPEYLAPE
Y316 tFCGTPEYLAPEVLE
Y327 EVLEDNDYGRAVDWW
K378 RTLGPEAKSLLAGLL
Y438 TSEVDTRYFDDEFTA
S447 DDEFTAQSITITPPD
T449 EFTAQSITITPPDRY
T451 TAQSITITPPDRYDS
Y456 TITPPDRYDSLGSLE
S458 TPPDRYDSLGSLELD
S474-p RTHFPQFsYSASIRE
Y475 THFPQFsYSASIRE_
S476 HFPQFsYSASIRE__
S478 PQFsYSASIRE____
13038 : Phospho-Akt (Thr308) (D25E6) XP(R) Rabbit mAb
9267 : Phospho-Akt (Thr450) Antibody
12178 : Phospho-Akt (Thr450) (D5G4) Rabbit mAb
2336 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
2337 : Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
3787 : Phospho-Akt (Ser473) (736E11) Rabbit mAb
4051 : Phospho-Akt (Ser473) (587F11) Mouse mAb
4058 : Phospho-Akt (Ser473) (193H12) Rabbit mAb
4060 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb
4070 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Sepharose Bead Conjugate)
4071 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4075 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5012 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Biotinylated)
5102 : Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
5315 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (PE Conjugate)
8599 : Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific)
9271 : Phospho-Akt (Ser473) Antibody
9563 : Phospho-Akt (Ser473) (D9E) XP(R) Rabbit mAb (Magnetic Bead Conjugate)
12694 : Phospho-Akt (Ser473) (D9W9U) Mouse mAb
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