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Protein Page:
MKK4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MKK4 dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; EC 2.7.12.2; STE group; STE7 family
Cellular Component: dendrite cytoplasm; perikaryon; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity
Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; signal transduction; activation of JNK activity; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication
Reference #:  P45985 (UniProtKB)
Alt. Names/Synonyms: c-Jun N-terminal kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; MAP2K4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; mitogen-activated protein kinase kinase 4; MKK4; MP2K4; PRKMK4; SAPK/ERK kinase 1; SEK1; SERK1
Gene Symbols: MAP2K4
Molecular weight: 44,288 Da
Basal Isoelectric point: 8.28  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MKK4

Protein Structure Not Found.


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Sites Implicated In
apoptosis, inhibited: S80‑p
transcription, induced: T261‑p
activity, inhibited: S80‑p
enzymatic activity, induced: S257‑p, T261‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S5-p ___MAAPsPsGGGGS
0 1 S7-p _MAAPsPsGGGGSGG
0 1 S18-p GSGGGSGsGTPGPVG
0 2 S26-p GTPGPVGsPAPGHPA
0 1 K54 NFANPPFKStArFTL
0 4 T56-p ANPPFKStArFTLNP
0 4 R58-m1 PPFKStArFTLNPNP
0 4 R58-m2 PPFKStArFTLNPNP
0 11 T78-p PHIERLRtHsIESSG
5 92 S80-p IERLRtHsIESSGKL
0 1 S84 RtHsIESSGKLKIsP
0 13 S90-p SSGKLKIsPEQHWDF
0 1 K103 DFTAEDLKDLGEIGR
0 2 K131-ac SGQIMAVkRIRSTVD
1 0 K140-ub IRSTVDEkEQkQLLM
1 0 K143-ub TVDEkEQkQLLMDLD
0 1 Y191 SFDKFYKYVYSVLDD
0 1 Y193 DKFYKYVYSVLDDVI
0 1 K220 VKALNHLKENLKIIH
12 45 S257-p ISGQLVDsIAKtRDA
23 3 T261-p LVDsIAKtRDAGCRP
0 4 T391 ILDQMPATPssPMYV
0 3 S393-p DQMPATPssPMYVD_
0 8 S394-p QMPATPssPMYVD__
0 1 Y397 ATPssPMYVD_____
9155 : Phospho-SEK1/MKK4 (Ser80) Antibody
4514 : Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
9151 : Phospho-SEK1/MKK4 (Thr261) Antibody
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
  mouse

 
S5 ___MAAPSPSGGGGS
S7 _MAAPSPSGGGGSGG
G16 GGGSGGGGGTPGPIG
P24 GTPGPIGPPASGHPA
K52-ub NFANPPVkStArFTL
T54-p ANPPVkStArFTLNP
R56 PPVkStARFTLNPNT
R56-m2 PPVkStArFTLNPNT
T76 PHIERLRTHsIESsG
S78-p IERLRTHsIESsGKL
S82-p RTHsIESsGKLKIsP
S88-p SsGKLKIsPEQHWDF
K101-ac DFTAEDLkDLGEIGR
K129 SGQIMAVKRIRSTVD
K138 IRSTVDEKEQKQLLM
K141 TVDEKEQKQLLMDLD
Y189 SFDKFYKYVYSVLDD
Y191 DKFYKYVYSVLDDVI
K218-ac VKALNHLkENLKIIH
S255-p ISGQLVDsIAKtRDA
T259-p LVDsIAKtRDAGCRP
T389-p ILDQMPAtPssPMyV
S391-p DQMPAtPssPMyVD_
S392-p QMPAtPssPMyVD__
Y395-p AtPssPMyVD_____
9155 : Phospho-SEK1/MKK4 (Ser80) Antibody
4514 : Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
9151 : Phospho-SEK1/MKK4 (Thr261) Antibody
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
  rat

 
S5 ___MAAPSPSGGGGS
S7 _MAAPSPSGGGGSGG
G16 GGGSGGGGGTPGPIG
P24 GTPGPIGPPASGHPA
K52 NFANPPVKSTArFTL
T54 ANPPVKSTArFTLNP
R56-m1 PPVKSTArFTLNPNT
R56 PPVKSTARFTLNPNT
T76 PHIERLRTHSIESSG
S78 IERLRTHSIESSGKL
S82 RTHSIESSGKLKIsP
S88-p SSGKLKIsPEQHWDF
K101 DFTAEDLKDLGEIGR
K129-ac SGQIMAVkRIRSTVD
K138 IRSTVDEKEQKQLLM
K141 TVDEKEQKQLLMDLD
Y189-p SFDKFYKyVySVLDD
Y191-p DKFYKyVySVLDDVI
K218-ac VKALNHLkENLKIIH
S255 ISGQLVDSIAKtRDA
T259-p LVDSIAKtRDAGCRP
T389-p ILDQMPAtPSSPMYV
S391 DQMPAtPSSPMYVD_
S392 QMPAtPSSPMYVD__
Y395 AtPSSPMYVD_____
4514 : Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
9151 : Phospho-SEK1/MKK4 (Thr261) Antibody
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
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