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Protein Page:
Pyk2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Pyk2 a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Nuclear receptor co-regulator; EC 2.7.10.2; Kinase, protein; TK group; Fak family
Cellular Component: focal adhesion; dendrite; postsynaptic density; cell cortex; cytosol; N-methyl-D-aspartate selective glutamate receptor complex; lipid raft; growth cone; cell soma; perinuclear region of cytoplasm; axon; lamellipodium; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; signal transducer activity; calmodulin-dependent protein kinase activity; 3-phosphoinositide-dependent protein kinase binding; N-methyl-D-aspartate selective glutamate receptor activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein complex binding; ATP binding
Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; glial cell proliferation; regulation of inositol trisphosphate biosynthetic process; negative regulation of bone mineralization; regulation of cell shape; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; response to glucose stimulus; protein complex assembly; negative regulation of neuron apoptosis; neurite development; bone resorption; response to drug; negative regulation of myeloid cell differentiation; activation of Rac GTPase; positive regulation of nitric-oxide synthase activity; response to osmotic stress; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to calcium ion; negative regulation of apoptosis; activation of JAK protein; response to cAMP; peptidyl-tyrosine phosphorylation; blood vessel endothelial cell migration; positive regulation of translation; apoptosis; response to lithium ion; negative regulation of potassium ion transport; protein amino acid autophosphorylation; response to hormone stimulus; positive regulation of JNK cascade; regulation of calcium-mediated signaling; signal transduction; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; response to stress; positive regulation of cell-matrix adhesion; regulation of cell adhesion; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; response to cocaine; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; response to hydrogen peroxide; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; response to hypoxia; stress fiber formation; cellular defense response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration
Reference #:  Q14289 (UniProtKB)
Alt. Names/Synonyms: CADTK; CAK beta; CAK-beta; CAKB; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; FADK 2; FADK2; FAK2; Focal adhesion kinase 2; PKB; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; Protein-tyrosine kinase 2-beta; PTK; PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; Related adhesion focal tyrosine kinase
Gene Symbols: PTK2B
Molecular weight: 115,875 Da
Basal Isoelectric point: 5.91  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Pyk2

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: Y402‑p
cell motility, altered: Y402‑p
enzymatic activity, induced: Y402‑p
intracellular localization: Y402‑p
molecular association, regulation: Y402‑p, Y881‑p, Y906‑p
phosphorylation: Y402‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S2-p ______MsGVSEPLs
0 1 S9-p sGVSEPLsRVKLGtL
0 11 T15-p LsRVKLGtLRRPEGP
0 8 Y46 RILKVCFYSNSFNPG
0 1 S47 ILKVCFYSNSFNPGK
0 1 T150 SLKEDRTTLLYFYQQ
0 1 Y153 EDRTTLLYFYQQLRN
0 1 K188-ub LELRRFFkDMPHNAL
0 2 K206-ub SNFELLEkEVGLDLF
0 1 S332-p EGAPQALsIKTSSLA
0 2 Y351 MADLIDGYCRLQGEH
0 1 S361-p LQGEHQGsLIIHPRK
0 31 S375-p KDGEKRNsLPQIPML
0 1 M381 NsLPQIPMLNLEARR
0 1 S394-p RRSHLSEsCsIEsDI
0 2 S396-p SHLSEsCsIEsDIyA
0 2 S399-p SEsCsIEsDIyAEIP
62 25 Y402-p CsIEsDIyAEIPDEt
0 2 T409-p yAEIPDEtLRRPGGP
0 4 Y418-p RRPGGPQyGIAREDV
0 11 Y440-p EGFFGEVyEGVYTNH
0 3 Y444 GEVyEGVYTNHKGEK
0 1 S473-p DNKEKFMsEAVIMKN
0 3 S530-p VLTLVLYsLQICKAM
0 1 S559-p VRNILVAsPECVkLG
0 3 K564-ub VAsPECVkLGDFGLs
0 2 K564-ac VAsPECVkLGDFGLs
0 7 S571-p kLGDFGLsRyIEDED
0 2 Y573-p GDFGLsRyIEDEDyy
6 960 Y579-p RyIEDEDyyKAsVTR
15 978 Y580-p yIEDEDyyKAsVTRL
0 2 S583-p DEDyyKAsVTRLPIK
0 2 Y683-p VCSLSDVyQMEKDIA
0 2 Y699-p EQERNARyRtPKILE
0 1 T701-p ERNARyRtPKILEPT
0 1 S718-p QEPPPKPsRPKyRPP
0 9 Y722-p PKPsRPKyRPPPQTN
0 4 S747-p PEGLCASsPtLTsPM
0 1 T749-p GLCASsPtLTsPMEy
0 2 S752-p ASsPtLTsPMEyPsP
0 3 Y756-p tLTsPMEyPsPVNsL
0 5 S758-p TsPMEyPsPVNsLHt
0 4 S762-p EyPsPVNsLHtPPLH
0 6 T765-p sPVNsLHtPPLHRHN
1 5 S778-p HNVFKRHsMREEDFI
0 39 Y819-p QKQMVEDyQWLRQEE
0 1 S828-p WLRQEEKsLDPMVyM
0 7 Y834-p KsLDPMVyMNDKsPL
0 6 S839-p MVyMNDKsPLtPEKE
0 19 T842-p MNDKsPLtPEKEVGy
0 15 Y849-p tPEKEVGyLEFtGPP
0 1 L850 PEKEVGyLEFtGPPQ
0 1 T853-p EVGyLEFtGPPQkPP
0 3 K858-ub EFtGPPQkPPRLGAQ
0 1 S866-p PPRLGAQsIQPTANL
17 1 Y881-p DRTDDLVyLNVMELV
1 0 Y906-p CQLPPEGyVVVVkNV
0 3 K911-ub EGyVVVVkNVGLTLR
0 1 S923-p TLRKLIGsVDDLLPS
0 1 K944-ub TEIEGTQkLLNKDLA
0 1 K956 DLAELINKMRLAQQN
0 1 K973-ub TSLSEECkRQMLTAS
3291 : Phospho-Pyk2 (Tyr402) Antibody
  Pyk2 iso2  
S2 ______MSGVSEPLS
S9 SGVSEPLSRVKLGTL
T15 LSRVKLGTLRRPEGP
Y46 RILKVCFYSNSFNPG
S47 ILKVCFYSNSFNPGK
T150 SLKEDRTTLLYFYQQ
Y153 EDRTTLLYFYQQLRN
K188 LELRRFFKDMPHNAL
K206 SNFELLEKEVGLDLF
S332 EGAPQALSIKTSSLA
Y351 MADLIDGYCRLQGEH
S361 LQGEHQGSLIIHPRK
S375 KDGEKRNSLPQIPML
M381 NSLPQIPMLNLEARR
S394 RRSHLSESCSIESDI
S396 SHLSESCSIESDIYA
S399 SESCSIESDIYAEIP
Y402 CSIESDIYAEIPDET
T409 YAEIPDETLRRPGGP
Y418 RRPGGPQYGIAREDV
Y440 EGFFGEVYEGVYTNH
Y444 GEVYEGVYTNHKGEK
S473 DNKEKFMSEAVIMKN
S530 VLTLVLYSLQICKAM
S559 VRNILVASPECVKLG
K564 VASPECVKLGDFGLS
K564 VASPECVKLGDFGLS
S571 KLGDFGLSRYIEDED
Y573 GDFGLSRYIEDEDYY
Y579 RYIEDEDYYKASVTR
Y580 YIEDEDYYKASVTRL
S583 DEDYYKASVTRLPIK
Y683 VCSLSDVYQMEKDIA
Y699 EQERNARYRTPKILE
T701 ERNARYRTPKILEPT
S718 QEPPPKPSRPKYRPP
Y722 PKPSRPKYRPPPQTN
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y777 QKQMVEDYQWLRQEE
S786 WLRQEEKSLDPMVYM
Y792 KSLDPMVYMNDKSPL
S797 MVYMNDKSPLTPEKE
T800 MNDKSPLTPEKEVGY
Y807 TPEKEVGYLEFTGPP
L808 PEKEVGYLEFTGPPQ
T811 EVGYLEFTGPPQKPP
K816 EFTGPPQKPPRLGAQ
S824 PPRLGAQSIQPTANL
Y839 DRTDDLVYLNVMELV
Y864 CQLPPEGYVVVVKNV
K869 EGYVVVVKNVGLTLR
S881 TLRKLIGSVDDLLPS
K902 TEIEGTQKLLNKDLA
K914 DLAELINKMRLAQQN
K931 TSLSEECKRQMLTAS
  mouse

 
S2 ______MSGVSEPLS
S9 SGVSEPLSRVKVGTL
T15 LSRVKVGTLRRPEGP
Y46 RILKVCFYSNSFNPG
S47 ILKVCFYSNSFNPGK
T150 SLKEDRTTLLYFYQQ
Y153 EDRTTLLYFYQQLRN
K188 LELRRFFKDMPHNAL
K206 SNFELLEKEVGLDLF
S332 EGAPQSLSIKTSSLA
Y351 MADLIDGYCRLQGEH
S361 LQGEHKGSLIMHAKK
S375-p KDGEKRNsLPQIPtL
T381-p NsLPQIPtLNLEARR
S394 RRSHLSESCSIESDI
S396 SHLSESCSIESDIyA
S399 SESCSIESDIyAEIP
Y402-p CSIESDIyAEIPDET
T409 yAEIPDETLRRPGGP
Y418 RRPGGPQYGVAREEV
Y440-p EGFFGEVyEGVYTNH
Y444 GEVyEGVYTNHKGEK
S473 DNKEKFMSEAVIMKN
T530 VPTLVLYTLQICKAM
S559 VRNILVASPECVKLG
K564 VASPECVKLGDFGLs
K564 VASPECVKLGDFGLs
S571-p KLGDFGLsRyIEDED
Y573-p GDFGLsRyIEDEDyy
Y579-p RyIEDEDyyKASVTR
Y580-p yIEDEDyyKASVTRL
S583 DEDyyKASVTRLPIK
Y683 VCSLSDIYQMEKDIA
Y699 EQERNARYRPPKILE
P701 ERNARYRPPKILEPT
S718 QEPPPKPSRPKYRPP
Y722 PKPSRPKYRPPPQTN
S747-p PEGLCASsPTLTSPM
T749 GLCASsPTLTSPMEy
S752 ASsPTLTSPMEyPsP
Y756-p TLTSPMEyPsPVNSL
S758-p TSPMEyPsPVNSLHt
S762 EyPsPVNSLHtPPLH
T765-p sPVNSLHtPPLHRHN
S778 HNVFKRHSMREEDFI
S819 QKQMVEDSQWLRREE
C828 WLRREERCLDPMVYM
Y834 RCLDPMVYMNDKSPL
S839 MVYMNDKSPLtPEKE
T842-p MNDKSPLtPEKEAGy
Y849-p tPEKEAGytEFTGPP
T850-p PEKEAGytEFTGPPQ
T853 EAGytEFTGPPQKPP
K858 EFTGPPQKPPRLGAQ
S866 PPRLGAQSIQPTANL
Y881-p DRTDDLVyHNVMTLV
Y906 GQLPPEDYVVVVKNV
K911 EDYVVVVKNVGLNLR
S923 NLRKLIGSVDDLLPS
K944 TEIEGTQKLLNKDLA
K956-ac DLAELINkMKLAQQN
K973 TSLSEDCKRQMLTAS
3291 : Phospho-Pyk2 (Tyr402) Antibody
  rat

 
S2 ______MSGVSEPLS
S9 SGVSEPLSRVKVGTL
T15 LSRVKVGTLRPPEGP
Y46-p RILKVCFysNSFNPG
S47-p ILKVCFysNSFNPGK
T150-p SLKEDRTtLLyFYQQ
Y153-p EDRTtLLyFYQQLRN
K188 LELRRFFKDMPHNAL
K206 SNFELLEKEVGLDLF
S332 EGAPQSLSIKTSSLA
Y351-p MADLIDGyCRLQGEH
S361 LQGEHKGSLIIHAKK
S375-p KDGEKRNsLPQIPTL
T381 NsLPQIPTLNLESRR
S394 RRSHLSESCSIESDI
S396 SHLSESCSIESDIyA
S399 SESCSIESDIyAEIP
Y402-p CSIESDIyAEIPDET
T409 yAEIPDETLRRPGGP
Y418-p RRPGGPQyGVAREDV
Y440-p EGFFGEVyEGVyTNH
Y444-p GEVyEGVyTNHKGEK
S473 DNKEKFMSEAVIMKN
A530 VPTLVLYALQICKAM
S559 VRNILVASPECVKLG
K564 VASPECVKLGDFGLs
K564 VASPECVKLGDFGLs
S571-p KLGDFGLsRYIEDED
Y573 GDFGLsRYIEDEDyy
Y579-p RYIEDEDyyKASVTR
Y580-p YIEDEDyyKASVTRL
S583 DEDyyKASVTRLPIK
Y683 VCSLSDIYQMERDIA
Y699 EQERNARYRPPKILE
P701 ERNARYRPPKILEPT
S718 QEPPPKPSRPKYKHP
Y722 PKPSRPKYKHPPQTN
S747 PEGLCASSPTLTSPM
T749 GLCASSPTLTSPMEY
S752 ASSPTLTSPMEYPSP
Y756 TLTSPMEYPSPVNSL
S758 TSPMEYPSPVNSLHT
S762 EYPSPVNSLHTPPLH
T765 SPVNSLHTPPLHRHN
S778 HNVFKRHSMREEDFI
S819 QKQMVEDSQWLRREE
C828 WLRREERCLDPMVyM
Y834-p RCLDPMVyMNDKsPL
S839-p MVyMNDKsPLTPEKE
T842 MNDKsPLTPEKEAGy
Y849-p TPEKEAGyTEFTGPP
T850 PEKEAGyTEFTGPPQ
T853 EAGyTEFTGPPQKPP
K858 EFTGPPQKPPRLGAQ
S866 PPRLGAQSIQPTANL
Y881-p DRTDDLVyHNVMTLV
Y906 SQLPPEEYVVVVKNV
K911 EEYVVVVKNVGLNLR
S923 NLRKLIGSVDDLLPS
K944 TEIEGTQKLLNKDLA
K956 DLAELINKMRLAQQN
K973 TSLSEDCKRQMLTAS
3291 : Phospho-Pyk2 (Tyr402) Antibody
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