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Protein Page:
PKN2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PKN2 an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase and Rho/Rac effector protein. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcriptional activation. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in an ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication. Kinase activity is activated upon binding to GTP-bound Rhoa/Rac1 GTPases. Activated by limited proteolysis with trypsin, and withn caspase-3 (CASP3) during apoptosis. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm. Five isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.13; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKN family
Chromosomal Location of Human Ortholog: 1p22.2
Cellular Component: apical junction complex; centrosome; intermediate filament cytoskeleton; membrane; lamellipodium; cytoplasm; nucleolus; midbody; nucleus; cleavage furrow
Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; histone deacetylase binding; ATP binding; protein kinase activity
Biological Process: positive regulation of cytokinesis; apical junction assembly; regulation of transcription, DNA-dependent; transcription, DNA-dependent; cell division; apoptosis; cell cycle; cell adhesion; positive regulation of mitotic cell cycle; signal transduction; protein amino acid phosphorylation
Reference #:  Q16513 (UniProtKB)
Alt. Names/Synonyms: cardiolipin-activated protein kinase Pak2; MGC150606; MGC71074; Pak-2; PAK2; PKN2; PRK2; PRKCL2; PRO2042; Protein kinase C-like 2; protein kinase N2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2
Gene Symbols: PKN2
Molecular weight: 112,035 Da
Basal Isoelectric point: 5.95  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PKN2

Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: T814‑p, S815‑p, T816‑p, T958‑p
enzymatic activity, inhibited: T958‑p
molecular association, regulation: T958‑p
protein conformation: T958‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 S3-p _____MAsNPERGEI
0 2 S21-p ELQGDSRsLPFSENV
0 1 S29-p LPFSENVsAVQKLDF
0 1 K58-ac RIKREIRkELKIKEG
0 1 K76 LRKVTTDKKSLAYVD
0 1 K87 AYVDNILKKSNKKLE
0 11 S110-p LNAHIVVsDPEDITD
0 3 T121-p DITDCPRtPDtPNND
0 4 T124-p DCPRtPDtPNNDPRC
0 1 S163-p ENMIQMYsNGSSKDR
0 1 S255-p VTDRKALsEAQARFN
0 1 S264-p AQARFNEsSQKLDLL
0 1 K272-ac SQKLDLLkysLEQRL
0 1 Y273-p QKLDLLkysLEQRLN
0 1 S274-p KLDLLkysLEQRLNE
0 2 S289-p VPKNHPKsRIIIEEL
0 7 S302-p ELSLVAAsPtLsPRQ
0 1 T304-p SLVAAsPtLsPRQSM
0 11 S306-p VAAsPtLsPRQSMIS
0 1 Y318-p MISTQNQySTLSKPA
0 1 S349-p LENVPGRsKATSVAL
0 12 S360-p SVALPGWsPsETRSs
0 1 S362-p ALPGWsPsETRSsFM
0 2 S367-p sPsETRSsFMsRTSK
0 2 S370-p ETRSsFMsRTSKSKS
0 1 S379-p TSKSKSGsSRNLLKT
0 1 S408-p NTVVGQTsWKPIsNQ
0 1 S413-p QTsWKPIsNQsWDQK
0 1 S416-p WKPIsNQsWDQKFTL
0 5 S497-p QRQKKIFsKQQGKTF
0 3 T516-p QMNINIAtWGRLVRR
0 2 S531-p AIPTVNHsGtFsPQA
0 1 T533-p PTVNHsGtFsPQAPV
1 9 S535-p VNHsGtFsPQAPVPT
0 1 A538 sGtFsPQAPVPTTVP
0 1 S559-p PQLAPPAsDsTVTKL
0 1 S561-p LAPPAsDsTVTKLDF
0 10 S582-p PPAPPRAssLGEIDE
0 36 S583-p PAPPRAssLGEIDES
0 2 S615-p DIQNDRNsILPKSQS
0 3 S620 RNsILPKSQSEyKPD
0 3 S622 sILPKSQSEyKPDtP
0 7 Y624-p LPKSQSEyKPDtPQs
0 8 T628-p QSEyKPDtPQsGLEy
0 9 S631-p yKPDtPQsGLEysGI
0 99 Y635-p tPQsGLEysGIQELE
0 8 S636-p PQsGLEysGIQELED
0 3 S646-p QELEDRRsQQRFQFN
0 4 Y676-p GKVLLAEyKNTNEMF
0 1 K686-ac TNEMFAIkALKKGDI
0 1 S701-p VARDEVDsLMCEKRI
0 1 K784-ub KIVYRDLkLDNLLLD
0 3 Y810-p LCKEGMGyGDRtstF
1 74 T814-p GMGyGDRtstFCGtP
1 39 S815-p MGyGDRtstFCGtPE
3 287 T816-p GyGDRtstFCGtPEF
0 4 T820-p RtstFCGtPEFLAPE
0 1 K930 ALMDKKVKPPFIPTI
0 1 S944 IRGREDVSNFDDEFT
0 1 S952-p NFDDEFTsEAPILtP
2 45 T958-p TsEAPILtPPREPRI
2611 : Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody
  PKN2 iso2  
S3 _____MASNPERGEI
S21 ELQGDSRSLPFSENV
S29 LPFSENVSAVQKLDF
K58 RIKREIRKELKIKEG
K76 LRKVTTDKKSLAYVD
K87 AYVDNILKKSNKKLE
S110 LNAHIVVSDPEDITD
T121 DITDCPRTPDTPNND
T124 DCPRTPDTPNNDPRC
S163 ENMIQMYSNGSSKDR
S255 VTDRKALSEAQARFN
S264 AQARFNESSQKLDLL
K272 SQKLDLLKYSLEQRL
Y273 QKLDLLKYSLEQRLN
S274 KLDLLKYSLEQRLNE
S289 VPKNHPKSRIIIEEL
S302 ELSLVAASPTLSPRQ
T304 SLVAASPTLSPRQSM
S306 VAASPTLSPRQSMIS
Y318 MISTQNQYSTLSKPA
S349 LENVPGRSKATSVAL
S360-p SVALPGWsPSETRSS
S362 ALPGWsPSETRSSFM
S367 sPSETRSSFMSRTSK
S370 ETRSSFMSRTSKNDV
- gap
S392 NTVVGQTSWKPISNQ
S397 QTSWKPISNQSWDQK
S400 WKPISNQSWDQKFTL
S481 QRQKKIFSKQQGKTF
T500 QMNINIATWGRLVRR
S515 AIPTVNHSGTFSPQA
T517 PTVNHSGTFSPQAPV
S519 VNHSGTFSPQAPVPT
A522 SGTFSPQAPVPTTVP
S543 PQLAPPASDSTVTKL
S545 LAPPASDSTVTKLDF
S566 PPAPPRASSLGEIDE
S567 PAPPRASSLGEIDES
S599 DIQNDRNSILPKSQS
S604 RNSILPKSQSEYKPD
S606 SILPKSQSEYKPDTP
Y608 LPKSQSEYKPDTPQS
T612 QSEYKPDTPQSGLEY
S615 YKPDTPQSGLEYSGI
Y619 TPQSGLEYSGIQELE
S620 PQSGLEYSGIQELED
S630 QELEDRRSQQRFQFN
Y660 GKVLLAEYKNTNEMF
K670 TNEMFAIKALKKGDI
S685 VARDEVDSLMCEKRI
K768 KIVYRDLKLDNLLLD
Y794 LCKEGMGYGDRTSTF
T798 GMGYGDRTSTFCGTP
S799 MGYGDRTSTFCGTPE
T800 GYGDRTSTFCGTPEF
T804 RTSTFCGTPEFLAPE
K914 ALMDKKVKPPFIPTI
S928 IRGREDVSNFDDEFT
S936 NFDDEFTSEAPILTP
T942 TSEAPILTPPREPRI
  mouse

 
S3-p _____MAsNPDRGEI
T21 ELQGDSRTLPFSENV
S29 LPFSENVSAVQKLDF
K58 RIKREIRKELKIKEG
K76-ub LRKVTTDkKNLAYVD
K87-ub AYVDNILkKSNKKLE
S110-p LNAHIVVsDPEDSTD
T121 DSTDCPRTPDTPNSD
T124 DCPRTPDTPNSDSRS
S163 ENMIQMYSNGSSKDR
S255 VTDRKALSEAQARFN
S264 AQARFNESSQKLDLL
K272 SQKLDLLKYSLEQRL
Y273 QKLDLLKYSLEQRLN
S274 KLDLLKYSLEQRLNE
S289 LPRNHPKSSVVIEEL
S301 EELSLVASPTLSPRQ
T303 LSLVASPTLSPRQSM
S305 LVASPTLSPRQSMLS
Y317 MLSTQNQYSTLSKPA
S348 LENVPGRSKATSVAL
S359-p SVALPGWsPSDNRSS
S361 ALPGWsPSDNRSSFM
S366 sPSDNRSSFMsRTSK
S369-p DNRSSFMsRTSKSKS
S378 TSKSKSGSSRNLLKT
S407 NTVVGQTSWKPISNQ
S412 QTSWKPISNQSWDQK
S415 WKPISNQSWDQKFTL
S496-p QRQKKIFsKQQGKTF
T515 QMNINIATWGRLVRR
S530 AIPTVNHSGTFsPQt
T532 PTVNHSGTFsPQtPV
S534-p VNHSGTFsPQtPVPA
T537-p SGTFsPQtPVPATVP
S558 PDLAPPASDSTVTKL
S560 LAPPASDSTVTKLDF
S581-p PPAPPRAssLGETDE
S582-p PAPPRAssLGETDES
N614 NIENDRNNLRPKsKs
S619-p RNNLRPKsKsEYELS
S621-p NLRPKsKsEYELSIP
Y623 RPKsKsEYELSIPDS
I627 KsEYELSIPDSGRSC
S630 YELSIPDSGRSCWGV
C634 IPDSGRSCWGVGELD
W635 PDSGRSCWGVGELDD
A645 GELDDKRAQQRFQFS
Y675 GKVLLAEYKHTNEMF
K685 TNEMFAIKALKKGDI
S700 VARDEVDSLMCEKRI
K783 KIVYRDLKLDNLLLD
Y809-p LCKEGMGyGDRtstF
T813-p GMGyGDRtstFCGtP
S814-p MGyGDRtstFCGtPE
T815-p GyGDRtstFCGtPEF
T819-p RtstFCGtPEFLAPE
K929-ub ALMDKKVkPPFVPTI
S943-p IRGREDVsNFDDEFT
S951 NFDDEFTSEAPILtP
T957-p TSEAPILtPPREPRI
  rat

 
S3 _____MASNPDRGEI
P21 ELQVDSRPLPFSENV
S29 LPFSENVSAVQKLDF
K58 RIKREIRKELKIKEG
K76 LRKVTTDKKNLAYVD
K87 AYVDNILKKSNKKLE
S110 LNAHIVVSDPEDYTD
T121 DYTDCPRTPDTPNSD
T124 DCPRTPDTPNSDSRS
S164 ENMIQMYSNGPSKDR
S257 VTDRKALSEAQARFN
S266 AQARFNESSQKLDLL
K274 SQKLDLLKYSLEQRL
Y275 QKLDLLKYSLEQRLN
S276 KLDLLKYSLEQRLNE
S291 LPKNHPKSSVVIEEL
S303 EELSLVASPTLSPRQ
T305 LSLVASPTLSPRQSM
S307 LVASPTLSPRQSMLS
Y319 MLSTQNQYSTLSKPA
S350 WENVPGRSKATSVAL
S361 SVALPGWSPSENRSS
S363 ALPGWSPSENRSSFM
S368 SPSENRSSFMSRTSK
S371 ENRSSFMSRTSKSKS
S380 TSKSKSGSSRNLLKT
I409 NTVVGQTIWKPISNQ
S414 QTIWKPISNQSWDQK
S417 WKPISNQSWDQKFTL
S498 QRQKKIFSKQQGKTF
T517 QMNINIATWGRLVRR
S532 AIPTVNHSGTFSPQT
T534 PTVNHSGTFSPQTPV
S536 VNHSGTFSPQTPVPA
T539 SGTFSPQTPVPATVP
S560 PELAPPASDSTVTKL
S562 LAPPASDSTVTKLDF
S583-p PPAPPRAssLGEIDD
S584-p PAPPRAssLGEIDDS
N616 DIENDRNNMRPKSKS
S621 RNNMRPKSKSEYELN
S623 NMRPKSKSEYELNIP
Y625 RPKSKSEYELNIPDS
I629 KSEYELNIPDSSRSC
S632 YELNIPDSSRSCWSV
C636 IPDSSRSCWSVGELE
W637 PDSSRSCWSVGELED
S647 GELEDKRSQQRFQFN
Y677 GKVLLAEYKHTNEMF
K687 TNEMFAIKALKKGDI
S702 VARDEVDSLMCEKRI
K785 KIVYRDLKLDNLLLD
Y811 LCKEGMGYGDRtStF
T815-p GMGYGDRtStFCGTP
S816 MGYGDRtStFCGTPE
T817-p GYGDRtStFCGTPEF
T821 RtStFCGTPEFLAPE
K931 ALLDKKVKPPFVPTI
S945 IRGREDVSNFDDEFT
S953 NFDDEFTSEAPILtP
T959-p TSEAPILtPPREPRI
2611 : Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody
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