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Protein Page:
PKN1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PKN1 an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); AGC group; PKN family
Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; midbody; nucleus; endosome; cleavage furrow
Molecular Function: protein kinase C activity; histone binding; histone deacetylase binding; Rac GTPase binding; protein kinase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; ligand-dependent nuclear receptor transcription coactivator activity; androgen receptor binding; GTP-Rho binding; chromatin binding; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; hyperosmotic response; transcription, DNA-dependent; signal transduction; protein amino acid phosphorylation; activation of JNK activity
Reference #:  Q16512 (UniProtKB)
Alt. Names/Synonyms: DBK; MGC46204; PAK1; PKN; PKN-ALPHA; PKN1; PRK1; PRKCL1; Protein kinase C-like 1; Protein kinase C-like PKN; protein kinase C-related kinase 1; protein kinase N1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine-threonine kinase N; Serine-threonine protein kinase N; serine/threonine protein kinase N; Serine/threonine-protein kinase N1
Gene Symbols: PKN1
Molecular weight: 103,932 Da
Basal Isoelectric point: 5.99  Predict pI for various phosphorylation states
Select Structure to View Below

PKN1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cytoskeletal reorganization: T774‑p
enzymatic activity, induced: T774‑p
molecular association, regulation: T774‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S8 MASDAVQSEPRSWSL
0 13 T63-p AENLRRAttDLGRsL
1 8 T64-p ENLRRAttDLGRsLG
0 1 S69-p AttDLGRsLGPVELL
0 1 S80-p VELLLRGsSRRLDLL
0 2 S112-p ATHDGPQsPGAGGPT
0 2 S205-p APDDTQGsPDLGAVE
0 1 S240-p KNVLRLLsAAKAPDR
0 1 K263-u KLTESNQkLGLLREA
0 1 A270 kLGLLREALERRLGE
0 1 T353-p PSMGGPGtPDSRPPF
0 1 R366-m1 PFLSRPArGLYSRsG
0 2 S372-p ArGLYSRsGsLSGRs
2 3 S374-p GLYSRsGsLSGRssL
0 1 S379-p sGsLSGRssLKAEAE
0 1 S380-p GsLSGRssLKAEAEN
0 1 K448-a LCALKFLkLEDFLDN
1 0 A501 FSKQQGKAFQRARQM
0 2 T529-p LIPNATGtGTFsPGA
1 0 T531 PNATGtGTFsPGAsP
0 15 S533-p ATGtGTFsPGAsPGs
0 15 S537-p GTFsPGAsPGsEART
0 1 S540-p sPGAsPGsEARTTGD
0 1 T557-p VEKLNLGtDsDssPQ
0 5 S559-p KLNLGtDsDssPQKS
0 4 S561-p NLGtDsDssPQKSSR
0 15 S562-p LGtDsDssPQKSSRD
1 0 P574 SRDPPSSPSsLSSPI
1 1 S576-p DPPSSPSsLSSPIQE
1 1 S608-p LCSPLRKsPLtLEDF
0 1 T611-p PLRKsPLtLEDFKFL
0 9 Y768-p LCKEGMGyGDRtstF
0 86 T772-p GMGyGDRtstFCGtP
0 46 S773-p MGyGDRtstFCGtPE
6 306 T774-p GyGDRtstFCGtPEF
1 9 T778-p RtstFCGtPEFLAPE
0 1 S902-p LSGRTDVsNFDEEFT
0 4 T914-p EFTGEAPtLsPPRDA
0 95 S916-p TGEAPtLsPPRDARP
2611 : Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody
  mouse

 
S8-p MAGDAVQsEPRSWSL
T63-p AENLRRAttDLGRSL
T64-p ENLRRAttDLGRSLA
S69 AttDLGRSLAPVELL
S80 VELLLRGSARRLDLL
S112 AGSDATQSLAEGSPI
D208 APDEAQGDPELGAVE
S243 KNVLRLLSGAKAPDR
K266 KLTESNQKLGLLREs
S273-p KLGLLREsLERRLGE
T356 PSVGASGTPESRTPF
R369-m1 PFLSRPArGLYSRsG
S375-p ArGLYSRsGSLSGRS
S377 GLYSRsGSLSGRSsL
S382 sGSLSGRSsLRGEAE
S383-p GSLSGRSsLRGEAEN
K451 LCALKFLKLEDFLDN
A504 FSKQQGKAFQRARQM
T532-p LIPSAVAtGTFsPNA
T534 PSAVAtGTFsPNAsP
S536-p AVAtGTFsPNAsPGA
S540-p GTFsPNAsPGAEIRH
A543 sPNAsPGAEIRHTGD
A560 MEKLNLGADSDSSSQ
S562 KLNLGADSDSSSQKS
S564 NLGADSDSSSQKSPP
S565 LGADSDSSSQKSPPG
S577 PPGLPSTSCsLSSPT
S579-p GLPSTSCsLSSPTHE
S612 LCSPLRKSPLTLEDF
T615 PLRKSPLTLEDFKFL
Y772-p LCKEGMGyGDRtstF
T776-p GMGyGDRtstFCGtP
S777-p MGyGDRtstFCGtPE
T778-p GyGDRtstFCGtPEF
T782-p RtstFCGtPEFLAPE
S906 LSGRTDVSNFDEEFT
T918-p EFTGEAPtLsPPRDA
S920-p TGEAPtLsPPRDARP
  rat

 
S8 MAGDAVQSEPRSWSL
T63 AENLRRATtDLGRSL
T64-p ENLRRATtDLGRSLA
S69 ATtDLGRSLAPVELL
S80 VELLLRGSARRLDLL
S112 AGSDAPQSLAEGSPV
D208 APDEAHGDPDLGAVE
S243 KNVLRLLSAAKAPDR
K266 KLTESNQKLGLLRES
S273 KLGLLRESLERRLGE
T356 PSVGASGTPDSRTPF
R369 PFLSRPARGLYNRSG
S375 ARGLYNRSGsLSGRS
S377-p GLYNRSGsLSGRSSL
S382 SGsLSGRSSLKGEAE
S383 GsLSGRSSLKGEAEN
K451 LCALKFLKLEDFLDN
T504-p FSKQQGQtFQRARQM
T532 LIPNAVATGsFSPNA
S534-p PNAVATGsFSPNASP
S536 AVATGsFSPNASPGS
S540 GsFSPNASPGSEIRS
S543 SPNASPGSEIRSTGD
A560 MEKLNLGADSDSSSQ
S562 KLNLGADSDSSSQKS
S564 NLGADSDSSSQKSPA
S565 LGADSDSSSQKSPAG
S577-p PAGLPSTsCsLSSPT
S579-p GLPSTsCsLSSPTHE
S612-p LCSPLRKsPLTLEDF
T615 PLRKsPLTLEDFKFL
Y772-p LCKEGMGyGDRtStF
T776-p GMGyGDRtStFCGTP
S777 MGyGDRtStFCGTPE
T778-p GyGDRtStFCGTPEF
T782 RtStFCGTPEFLAPE
S906 LSGRTDVSNFDEEFT
T918 EFTGEAPTLsPPRDA
S920-p TGEAPTLsPPRDARP
2611 : Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody
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