Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
PKN1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PKN1 an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.13; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKN family
Chromosomal Location of Human Ortholog: 19p13.12
Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; midbody; nucleus; endosome; cleavage furrow
Molecular Function: protein kinase C activity; histone binding; histone deacetylase binding; Rac GTPase binding; protein kinase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; GTP-Rho binding; chromatin binding; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; hyperosmotic response; transcription, DNA-dependent; signal transduction; protein amino acid phosphorylation; activation of JNK activity
Reference #:  Q16512 (UniProtKB)
Alt. Names/Synonyms: DBK; MGC46204; PAK1; PKN; PKN-ALPHA; PKN1; PRK1; PRKCL1; Protein kinase C-like 1; Protein kinase C-like PKN; protein kinase C-related kinase 1; protein kinase N1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine-threonine kinase N; Serine-threonine protein kinase N; serine/threonine protein kinase N; Serine/threonine-protein kinase N1
Gene Symbols: PKN1
Molecular weight: 103,932 Da
Basal Isoelectric point: 5.99  Predict pI for various phosphorylation states
Select Structure to View Below

PKN1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  KinBase  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
cytoskeletal reorganization: T774‑p
enzymatic activity, induced: T774‑p
molecular association, regulation: T774‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S3-p _____MAsDAVQSEP
0 1 S8 MAsDAVQSEPRSWSL
0 13 T63-p AENLRRAttDLGRsL
1 8 T64-p ENLRRAttDLGRsLG
0 3 S69-p AttDLGRsLGPVELL
0 1 S80-p VELLLRGsSRRLDLL
0 2 S112-p ATHDGPQsPGAGGPT
0 4 S205-p APDDTQGsPDLGAVE
0 1 S240-p KNVLRLLsAAKAPDR
0 1 K263-ub KLTESNQkLGLLREA
0 1 A270 kLGLLREALERRLGE
0 1 S301-p AASSAAFsTRLAGPF
0 1 T315-p FPATHYStLCKPAPL
0 1 T339 GCRDLPETIPWNPTP
0 1 N343 LPETIPWNPTPSMGG
0 1 S347 IPWNPTPSMGGPGtP
0 1 T353-p PSMGGPGtPDSRPPF
0 1 R366-m1 PFLSRPArGLYSRsG
0 2 S372-p ArGLYSRsGsLSGRs
2 3 S374-p GLYSRsGsLSGRssL
0 1 S379-p sGsLSGRssLKAEAE
0 2 S380-p GsLSGRssLKAEAEN
0 1 K448-ac LCALKFLkLEDFLDN
0 5 S495-p RRQKKIFsKQQGKAF
1 0 A501 FsKQQGKAFQRARQM
0 1 N525 LLRRLIPNAtGtGTF
0 1 T527-p RRLIPNAtGtGTFsP
0 3 T529-p LIPNAtGtGTFsPGA
1 0 T531 PNAtGtGTFsPGAsP
0 22 S533-p AtGtGTFsPGAsPGs
0 24 S537-p GTFsPGAsPGsEART
0 3 S540-p sPGAsPGsEARTTGD
0 2 S549-p ARTTGDIsVEKLNLG
0 2 T557-p VEKLNLGtDsDssPQ
0 9 S559-p KLNLGtDsDssPQKS
0 6 S561-p NLGtDsDssPQKSSR
0 22 S562-p LGtDsDssPQKSSRD
0 1 S573-p SSRDPPSsPssLSSP
1 0 P574 SRDPPSsPssLSSPI
0 1 S575-p RDPPSsPssLSSPIQ
1 2 S576-p DPPSsPssLSSPIQE
0 1 S579 SsPssLSSPIQESTA
0 1 S591 STAPELPSETQETPG
0 1 T593 APELPSETQETPGPA
0 1 S603-p TPGPALCsPLRKsPL
1 1 S608-p LCsPLRKsPLtLEDF
0 1 T611-p PLRKsPLtLEDFKFL
0 10 Y768-p LCKEGMGyGDRtstF
0 89 T772-p GMGyGDRtstFCGtP
1 47 S773-p MGyGDRtstFCGtPE
6 313 T774-p GyGDRtstFCGtPEF
1 12 T778-p RtstFCGtPEFLAPE
0 1 S902-p LSGRTDVsNFDEEFT
0 7 T914-p EFTGEAPtLsPPRDA
0 116 S916-p TGEAPtLsPPRDARP
0 1 T925-p PRDARPLtAAEQAAF
2611 : Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody
  PKN1 iso2  
- gap
S14 PSEQELESEPRSWSL
T69 AENLRRATTDLGRSL
T70 ENLRRATTDLGRSLG
S75 ATTDLGRSLGPVELL
S86 VELLLRGSSRRLDLL
S118 ATHDGPQSPGAGGPT
S211 APDDTQGSPDLGAVE
S246 KNVLRLLSAAKAPDR
K269 KLTESNQKLGLLREA
A276 KLGLLREALERRLGE
S307 AASSAAFSTRLAGPF
T321 FPATHYSTLCKPAPL
T345 GCRDLPETIPWNPTP
N349 LPETIPWNPTPSMGG
S353 IPWNPTPSMGGPGTP
T359 PSMGGPGTPDSRPPF
R372 PFLSRPARGLYSRSG
S378 ARGLYSRSGSLSGRS
S380 GLYSRSGSLSGRSSL
S385 SGSLSGRSSLKAEAE
S386 GSLSGRSSLKAEAEN
K454 LCALKFLKLEDFLDN
S501 RRQKKIFSKQQGKAF
A507 FSKQQGKAFQRARQM
N531 LLRRLIPNATGTGTF
T533 RRLIPNATGTGTFSP
T535 LIPNATGTGTFSPGA
T537 PNATGTGTFSPGASP
S539 ATGTGTFSPGASPGS
S543 GTFSPGASPGSEART
S546 SPGASPGSEARTTGD
S555 ARTTGDISVEKLNLG
T563 VEKLNLGTDSDSSPQ
S565 KLNLGTDSDSSPQKS
S567 NLGTDSDSSPQKSSR
S568 LGTDSDSSPQKSSRD
S579 SSRDPPSSPSSLSSP
P580 SRDPPSSPSSLSSPI
S581 RDPPSSPSSLSSPIQ
S582 DPPSSPSSLSSPIQE
S585 SSPSSLSSPIQESTA
S597 STAPELPSETQETPG
T599 APELPSETQETPGPA
S609 TPGPALCSPLRKSPL
S614 LCSPLRKSPLTLEDF
T617 PLRKSPLTLEDFKFL
Y774 LCKEGMGYGDRTSTF
T778 GMGYGDRTSTFCGTP
S779 MGYGDRTSTFCGTPE
T780 GYGDRTSTFCGTPEF
T784 RTSTFCGTPEFLAPE
S908 LSGRTDVSNFDEEFT
T920 EFTGEAPTLSPPRDA
S922 TGEAPTLSPPRDARP
T931 PRDARPLTAAEQAAF
  mouse

 
G3 _____MAGDAVQsEP
S8-p MAGDAVQsEPRSWSL
T63-p AENLRRAttDLGRSL
T64-p ENLRRAttDLGRSLA
S69 AttDLGRSLAPVELL
S80 VELLLRGSARRLDLL
S112 AGSDATQSLAEGSPI
D208 APDEAQGDPELGAVE
S243 KNVLRLLSGAKAPDR
K266 KLTESNQKLGLLREs
S273-p KLGLLREsLERRLGE
S304 AASSSAFSAILPGPF
T318 FPATHYSTLSKPAPL
T342-p GCKNLPEtIPWsPPP
S346-p LPEtIPWsPPPsVGA
S350-p IPWsPPPsVGASGTP
T356 PsVGASGTPESRTPF
R369-m1 PFLSRPArGLYSRsG
S375-p ArGLYSRsGSLSGRS
S377 GLYSRsGSLSGRSsL
S382 sGSLSGRSsLRGEAE
S383-p GSLSGRSsLRGEAEN
K451 LCALKFLKLEDFLDN
S498-p QRQKKIFsKQQGKAF
A504 FsKQQGKAFQRARQM
S528-p LLRRLIPsAVAtGTF
V530 RRLIPsAVAtGTFsP
T532-p LIPsAVAtGTFsPNA
T534 PsAVAtGTFsPNAsP
S536-p AVAtGTFsPNAsPGA
S540-p GTFsPNAsPGAEIRH
A543 sPNAsPGAEIRHTGD
S552 IRHTGDISMEKLNLG
A560 MEKLNLGADSDSSSQ
S562 KLNLGADSDSSSQKS
S564 NLGADSDSSSQKSPP
S565 LGADSDSSSQKSPPG
T576-p SPPGLPStSCsLSsP
S577 PPGLPStSCsLSsPT
C578 PGLPStSCsLSsPTH
S579-p GLPStSCsLSsPTHE
S582-p StSCsLSsPTHESTT
S595-p TTSPELPsEtQETPG
T597-p SPELPsEtQETPGPG
S607-p TPGPGLCsPLRKSPL
S612 LCsPLRKSPLTLEDF
T615 PLRKSPLTLEDFKFL
Y772-p LCKEGMGyGDRtstF
T776-p GMGyGDRtstFCGtP
S777-p MGyGDRtstFCGtPE
T778-p GyGDRtstFCGtPEF
T782-p RtstFCGtPEFLAPE
S906 LSGRTDVSNFDEEFT
T918-p EFTGEAPtLsPPRDA
S920-p TGEAPtLsPPRDARP
T929 PRDARPLTAAEQAAF
  rat

 
G3 _____MAGDAVQSEP
S8 MAGDAVQSEPRSWSL
T63 AENLRRATtDLGRSL
T64-p ENLRRATtDLGRSLA
S69 ATtDLGRSLAPVELL
S80 VELLLRGSARRLDLL
S112 AGSDAPQSLAEGSPV
D208 APDEAHGDPDLGAVE
S243 KNVLRLLSAAKAPDR
K266 KLTESNQKLGLLRES
S273 KLGLLRESLERRLGE
S304 AASSAAFSAILPGPF
T318 FPATHYSTLSKPAPL
T342 GCKNLPETIPWSPPP
S346 LPETIPWSPPPSVGA
S350 IPWSPPPSVGASGTP
T356 PSVGASGTPDSRTPF
R369 PFLSRPARGLYNRSG
S375 ARGLYNRSGsLSGRS
S377-p GLYNRSGsLSGRSSL
S382 SGsLSGRSSLKGEAE
S383 GsLSGRSSLKGEAEN
K451 LCALKFLKLEDFLDN
S498 QRQKKIFSKQQGQtF
T504-p FSKQQGQtFQRARQM
N528 LLRRLIPNAVATGsF
V530 RRLIPNAVATGsFSP
T532 LIPNAVATGsFSPNA
S534-p PNAVATGsFSPNASP
S536 AVATGsFSPNASPGS
S540 GsFSPNASPGSEIRS
S543 SPNASPGSEIRSTGD
S552 IRSTGDISMEKLNLG
A560 MEKLNLGADSDSSSQ
S562 KLNLGADSDSSSQKS
S564 NLGADSDSSSQKSPA
S565 LGADSDSSSQKSPAG
T576 SPAGLPSTsCsLSSP
S577-p PAGLPSTsCsLSSPT
C578 AGLPSTsCsLSSPTH
S579-p GLPSTsCsLSSPTHE
S582 STsCsLSSPTHESTT
S595 TTSPELPSETQETPG
T597 SPELPSETQETPGPG
S607 TPGPGLCSPLRKsPL
S612-p LCSPLRKsPLTLEDF
T615 PLRKsPLTLEDFKFL
Y772 LCKEGMGYGDRtStF
T776-p GMGYGDRtStFCGTP
S777 MGYGDRtStFCGTPE
T778-p GYGDRtStFCGTPEF
T782 RtStFCGTPEFLAPE
S906 LSGRTDVSNFDEEFT
T918 EFTGEAPTLsPPRDA
S920-p TGEAPTLsPPRDARP
T929 PRDARPLTAAEQAAF
2611 : Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.