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Protein Page:
PLK1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PLK1 a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.21; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family
Cellular Component: kinetochore; nucleoplasm; spindle pole; centrosome; spindle microtubule; cytoplasm; nucleolus; spindle; midbody; spindle midzone; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of proteolysis; regulation of cell cycle; regulation of mitotic cell cycle; mitotic nuclear envelope disassembly; protein ubiquitination; anaphase-promoting complex activation during mitotic cell cycle; cytokinesis; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to antibiotic; cytokinesis after mitosis; centrosome organization and biogenesis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity; mitotic cell cycle spindle assembly checkpoint; G2/M transition of mitotic cell cycle; mitosis; mitotic sister chromatid segregation; protein destabilization; regulation of mitotic metaphase/anaphase transition; negative regulation of cyclin-dependent protein kinase activity; cell proliferation; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; polar body extrusion after meiotic divisions; mitotic cell cycle; G2/M transition DNA damage checkpoint; microtubule bundle formation; mitotic metaphase/anaphase transition; negative regulation of apoptosis
Reference #:  P53350 (UniProtKB)
Alt. Names/Synonyms: cell cycle regulated protein kinase; PLK; PLK-1; PLK1; polo (Drosophia)-like kinase; polo like kinase; Polo-like kinase 1; polo-like kinase 1 (Drosophila); Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13
Gene Symbols: PLK1
Molecular weight: 68,255 Da
Basal Isoelectric point: 9.09  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PLK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell cycle regulation: S49‑p, S137‑p, T210‑p, S326‑p, S330‑p, S597‑p
enzymatic activity, induced: S49‑p, S137‑p, T210‑p, S326‑p
molecular association, regulation: T210‑p, S330‑p, S597‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S2-p ______MsAAVtAGK
0 2 T6-p __MsAAVtAGKLARA
1 1 A34 APGAPAAAPPAkEIP
1 1 P35 PGAPAAAPPAkEIPE
0 2 K38-u PAAAPPAkEIPEVLV
1 0 S49-p EVLVDPRsRRRYVRG
0 1 S103-p EKMSMEIsIHRSLAH
4 1 S137-p LELCRRRsLLELHKR
0 1 K200-u GDFGLATkVEYDGER
16 48 T210-p YDGERKKtLCGtPNy
0 10 T214-p RKKtLCGtPNyIAPE
0 150 Y217-p tLCGtPNyIAPEVLs
0 2 S224-p yIAPEVLsKKGHSFE
0 1 K272-u KNEYSIPkHINPVAA
1 0 S326-p LTIPPRFsIAPssLD
2 0 S330-p PRFsIAPssLDPsNR
0 2 S331-p RFsIAPssLDPsNRK
1 1 S335-p APssLDPsNRKPLtV
0 1 T341-p PsNRKPLtVLNKGLE
1 1 N349 VLNKGLENPLPERPR
0 1 S375-p EVVDCHLsDMLQQLH
1 0 S383-p DMLQQLHsVNAsKPS
0 1 S387-p QLHsVNAsKPSERGL
0 1 S450-p ILYNDGDsLQYIERD
0 1 S461-p IERDGTEsYLtVSSH
0 1 T464-p DGTEsYLtVSSHPNS
0 1 K474-u SHPNSLMkKITLLkY
0 1 K480-u MkKITLLkYFRNYMS
0 2 K492-u YMSEHLLkAGANItP
0 3 T498-p LkAGANItPREGDEL
0 1 K589-u YARTMVDkLLSSRSA
2 0 S597-p LLSSRSAsNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
9062 : Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb
  mouse

 
N2 ______MNAAAKAGK
K6 __MNAAAKAGKLARA
A34 VPGAPVAAPLAKEIP
P35 PGAPVAAPLAKEIPE
K38 PVAAPLAKEIPEVLV
S49 EVLVDPRSRRQYVRG
S103 EKMSMEISIHRSLAH
S137 LELCRRRSLLELHKR
K200 GDFGLATKVEYEGER
T210-p YEGERKKtLCGtPNY
T214-p RKKtLCGtPNYIAPE
Y217 tLCGtPNYIAPEVLS
S224 YIAPEVLSKKGHSFE
K272 KNEYSIPKHINPVAA
S326 LTIPPRFSIAPSsLD
S330 PRFSIAPSsLDPSSR
S331-p RFSIAPSsLDPSSRK
S335 APSsLDPSSRKPLKV
K341 PSSRKPLKVLNKGVE
N349 VLNKGVENPLPDRPR
S375 EAIECHLSDLLQQLT
S383 DLLQQLTSVNASKPS
S387 QLTSVNASKPSERGL
S450 ILYNDGDSLQYIERD
S461 IERDGTESYLTVSSH
T464 DGTESYLTVSSHPNS
K474 SHPNSLMKKITLLNY
N480 MKKITLLNYFRNYMS
K492 YMSEHLLKAGANITP
T498 LKAGANITPREGDEL
K589 YARTMVDKLLSSRSA
S597 LLSSRSASNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
  rat

 
N2 ______MNAAAKAGK
K6 __MNAAAKAGKLARA
A34 APGAPGAAPLAKEIP
P35 PGAPGAAPLAKEIPE
K38 PGAAPLAKEIPEVLM
S49 EVLMDPRSRRQYVRG
S103 EKMSMEISIHRSLAH
S137 LELCRRRSLLELHKR
K200 GDFGLATKVEYEGER
T210-p YEGERKKtLCGTPNY
T214 RKKtLCGTPNYIAPE
Y217 tLCGTPNYIAPEVLS
S224 YIAPEVLSKKGHSFE
K272 KNEYSIPKHINPVAA
S326 LTIPPRFSIAPSSLD
S330 PRFSIAPSSLDPSNR
S331 RFSIAPSSLDPSNRK
S335 APSSLDPSNRKPLTV
T341 PSNRKPLTVLNKGVE
N349 VLNKGVENPLPDRPR
S375 EAIECHLSDLLQQLT
S383 DLLQQLTSVNASKPS
S387 QLTSVNASKPSERGL
S450 ILYNDGDSLQYIERD
S461 IERDGTESYLTVSSH
T464 DGTESYLTVSSHPNS
K474 SHPNSLMKKITLLNY
N480 MKKITLLNYFRNYMS
K492 YMSEHLLKAGANITP
T498 LKAGANITPREGDEL
K589 YARTMVDKLLSSRSA
C597 LLSSRSACNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
  frog

 
- gap
- gap
S25-p PPGIPGSssAVKEIP
S26-p PGIPGSssAVKEIPE
K29 PGSssAVKEIPEILV
T40 EILVDPRTRRRYLRG
A94 DKMTMEIAIQRSLDH
S128 LELCRRRSLLELHKR
K191 GDFGLATKVEYDGER
T201 YDGERKKTLCGTPNY
T205 RKKTLCGTPNYIAPE
Y208 TLCGTPNYIAPEVLG
G215 YIAPEVLGKKGHSFE
K263 KNEYSIPKHINPVAA
S317 LTVPPRFSIAPSTID
S321 PRFSIAPSTIDQsLR
T322 RFSIAPSTIDQsLRK
S326-p APSTIDQsLRKPLTA
T332 QsLRKPLTAINKGQD
S340-p AINKGQDsPLVEKQV
S369 EPADCYLSEMLQQLT
C377 EMLQQLTCLNAVKPS
V381 QLTCLNAVKPSERAL
S444 IMYNDGDSLQYIERN
S455 IERNNTESYLNVRSY
N458 NNTESYLNVRSYPTT
K468 SYPTTLTKKITLLKY
K474 TKKITLLKYFRNYMS
K486 YMSEHLLKAGANTTP
T492 LKAGANTTPREGDEL
K583 YARTMVEKLQSSKSA
V591 LQSSKSAVAHVKASA
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
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