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PLK1 (human)

Overview PLK1 a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events. Note: This description may include information from UniProtKB. Protein type: EC 2.7.11.21; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family Cellular Component: kinetochore; nucleoplasm; spindle pole; centrosome; spindle microtubule; spindle; midbody; spindle midzone; nucleus; cytosol Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; protein kinase binding; ATP binding; protein kinase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic prophase; positive regulation of proteolysis; regulation of cell cycle; regulation of mitotic cell cycle; anaphase-promoting complex activation during mitotic cell cycle; protein ubiquitination; cytokinesis; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to antibiotic; M phase of mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; centrosome organization and biogenesis; positive regulation of ubiquitin-protein ligase activity; G2/M transition of mitotic cell cycle; mitotic prometaphase; mitosis; protein destabilization; negative regulation of cyclin-dependent protein kinase activity; cell proliferation; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; polar body extrusion after meiotic divisions; mitotic cell cycle; G2/M transition DNA damage checkpoint; mitotic metaphase/anaphase transition; microtubule bundle formation; negative regulation of apoptosis Reference #:  P53350 (UniProtKB) Alt. Names/Synonyms: cell cycle regulated protein kinase; PLK; PLK-1; PLK1; polo (Drosophia)-like kinase; polo like kinase; Polo-like kinase 1; polo-like kinase 1 (Drosophila); Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13 Gene Symbols: PLK1 Molecular weight: 68,255 Da Basal Isoelectric point: 9.09  Predict pI for various phosphorylation states CST Pathways:  Cell Cycle: G2/M DNA Damage Checkpoint Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PLK1 1Q4K - A/B/C=345-603 (human) 1Q4O - A/B=367-603 (human) 1UMW - A/B=367-603 (human) 2OGQ - A=365-603 (human) 2OJS - A=365-603 (human) 2OJX - A=365-603 (human) 2OU7 - A=13-345 (human) 2OWB - A=13-345 (human) 2RKU - A=37-330 (human) 2V5Q - A/B=33-345 (human) 2YAC - A=36-345 (human) 3BZI - A=365-603 (human) 3C5L - A=373-593 (human) 3FC2 - A=13-345 (human) 3FVH - A=371-603 (human) 3HIH - A/B=371-593 (human) 3HIK - A=367-603 (human) 3KB7 - A=36-345 (human) 3MHN - A/B=367-603 (human) 3MHQ - A/B=367-603 (human) 3MQ8 - A/B=367-603 (human) 3P2W - A=371-594 (human) 3P2Z - A=371-594 (human) 3P34 - A=371-594 (human) 3P35 - A/C/B=371-594 (human) 3P36 - A=371-594 (human) 3P37 - A/C/B=371-594 (human) 3Q1I - A=371-594 (human) 3RQ7 - A=371-603 (human) 3THB - A=13-345 (human) 4A4L - A=36-345 (human) 4A4O - A=36-345 (human) 4DFW - A=367-603 (human) 4E67 - A=371-594 (human) 4E9C - A=371-594 (human) 4E9D - A=371-594 (human) 4H5X - A/B=367-603 (human) 4H71 - A/B=367-603 (human) 4HAB - A/C/B=371-593 (human) 4HCO - A/B=367-603 (human) 4HY2 - A=371-595 (human) Substrate Sequence Logo

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1Q4K 1Q4O 1UMW 2OGQ 2OJS 2OJX 2OU7 2OWB 2RKU 2V5Q 2YAC 3BZI 3C5L 3FC2 3FVH 3HIH 3HIK 3KB7 3MHN 3MHQ 3MQ8 3P2W 3P2Z 3P34 3P35 3P36 3P37 3Q1I 3RQ7 3THB 4A4L 4A4O 4DFW 4E67 4E9C 4E9D 4H5X 4H71 4HAB 4HCO 4HY2  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  InnateDB  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
cell cycle regulation: S49‑p, S137‑p, T210‑p, S326‑p, S330‑p, S597‑p enzymatic activity, induced: S49‑p, S137‑p, T210‑p, S326‑p molecular association, regulation: T210‑p, S330‑p, S597‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	2		S2-p	______MsAAVtAGK
0	2		T6-p	__MsAAVtAGKLARA
1	1		A34	APGAPAAAPPAkEIP
1	1		P35	PGAPAAAPPAkEIPE
0	2		K38-u	PAAAPPAkEIPEVLV
1	0		S49-p	EVLVDPRsRRRYVRG
0	1		S103-p	EKMSMEIsIHRSLAH
4	1		S137-p	LELCRRRsLLELHKR
0	1		K146-u	LELHKRRkALTEPEA
0	1		K200-u	GDFGLATkVEYDGER
14	46		T210-p	YDGERKKtLCGtPNy
0	10		T214-p	RKKtLCGtPNyIAPE
0	150		Y217-p	tLCGtPNyIAPEVLs
0	1		S224-p	yIAPEVLsKKGHSFE
0	1		K272-u	KNEYSIPkHINPVAA
0	1		Y309-p	DEFFTSGyIPARLPI
1	0		S326-p	LTIPPRFsIAPsSLD
2	0		S330-p	PRFsIAPsSLDPsNR
0	1		S331	RFsIAPsSLDPsNRk
1	1		S335-p	APsSLDPsNRkPLtV
0	1		K338-u	SLDPsNRkPLtVLNK
0	1		T341-p	PsNRkPLtVLNKGLE
1	1		N349	VLNKGLENPLPERPR
0	1		S375-p	EVVDCHLsDMLQQLH
1	0		S383-p	DMLQQLHsVNAsKPS
0	1		S387-p	QLHsVNAsKPSERGL
0	1		S450-p	ILYNDGDsLQYIERD
0	1		S461-p	IERDGTEsYLtVsSH
0	1		T464-p	DGTEsYLtVsSHPNS
0	1		S466-p	TEsYLtVsSHPNSLM
0	1		K474-u	SHPNSLMkKITLLkY
0	1		K480-u	MkKITLLkYFRNYMS
0	2		K492-u	YMSEHLLkAGANItP
0	3		T498-p	LkAGANItPREGDEL
0	1		S565-p	DFRTYRLsLLEEYGC
0	1		S578-p	GCCKELAsRLRYART
0	1		K589-u	YARTMVDkLLSSRSA
2	0		S597-p	LLSSRSAsNRLKAS_

	mouse
N2	______MNAAAKAGK
K6	__MNAAAKAGKLARA
A34	VPGAPVAAPLAKEIP
P35	PGAPVAAPLAKEIPE
K38	PVAAPLAKEIPEVLV
S49	EVLVDPRSRRQYVRG
S103	EKMSMEISIHRSLAH
S137	LELCRRRSLLELHKR
K146	LELHKRRKALTEPEA
K200	GDFGLATKVEYEGER
T210-p	YEGERKKtLCGtPNY
T214-p	RKKtLCGtPNYIAPE
Y217	tLCGtPNYIAPEVLS
S224	YIAPEVLSKKGHSFE
K272	KNEYSIPKHINPVAA
Y309	DEFFTSGYIPARLPI
S326	LTIPPRFSIAPSsLD
S330	PRFSIAPSsLDPSSR
S331-p	RFSIAPSsLDPSSRK
S335	APSsLDPSSRKPLKV
K338	sLDPSSRKPLKVLNK
K341	PSSRKPLKVLNKGVE
N349	VLNKGVENPLPDRPR
S375	EAIECHLSDLLQQLT
S383	DLLQQLTSVNASKPS
S387	QLTSVNASKPSERGL
S450	ILYNDGDSLQYIERD
S461	IERDGTESYLTVSSH
T464	DGTESYLTVSSHPNS
S466	TESYLTVSSHPNSLM
K474	SHPNSLMKKITLLNY
N480	MKKITLLNYFRNYMS
K492	YMSEHLLKAGANITP
T498	LKAGANITPREGDEL
S565	DFQTYRLSLLEEYGC
S578	GCCKELASRLRYART
K589	YARTMVDKLLSSRSA
S597	LLSSRSASNRLKAS_

	rat
N2	______MNAAAKAGK
K6	__MNAAAKAGKLARA
A34	APGAPGAAPLAKEIP
P35	PGAPGAAPLAKEIPE
K38	PGAAPLAKEIPEVLM
S49	EVLMDPRSRRQYVRG
S103	EKMSMEISIHRSLAH
S137	LELCRRRSLLELHKR
K146	LELHKRRKALTEPEA
K200	GDFGLATKVEYEGER
T210-p	YEGERKKtLCGTPNY
T214	RKKtLCGTPNYIAPE
Y217	tLCGTPNYIAPEVLS
S224	YIAPEVLSKKGHSFE
K272	KNEYSIPKHINPVAA
Y309	DEFFTSGYIPARLPI
S326	LTIPPRFSIAPSSLD
S330	PRFSIAPSSLDPSNR
S331	RFSIAPSSLDPSNRK
S335	APSSLDPSNRKPLTV
K338	SLDPSNRKPLTVLNK
T341	PSNRKPLTVLNKGVE
N349	VLNKGVENPLPDRPR
S375	EAIECHLSDLLQQLT
S383	DLLQQLTSVNASKPS
S387	QLTSVNASKPSERGL
S450	ILYNDGDSLQYIERD
S461	IERDGTESYLTVSSH
T464	DGTESYLTVSSHPNS
S466	TESYLTVSSHPNSLM
K474	SHPNSLMKKITLLNY
N480	MKKITLLNYFRNYMS
K492	YMSEHLLKAGANITP
T498	LKAGANITPREGDEL
S565	DFRTYRLSLLEEYGC
S578	GCCKELASRLRYART
K589	YARTMVDKLLSSRSA
C597	LLSSRSACNRLKAS_

	frog
-	gap
-	gap
S25-p	PPGIPGSssAVKEIP
S26-p	PGIPGSssAVKEIPE
K29	PGSssAVKEIPEILV
T40	EILVDPRTRRRYLRG
A94	DKMTMEIAIQRSLDH
S128	LELCRRRSLLELHKR
K137	LELHKRRKAVTEPEA
K191	GDFGLATKVEYDGER
T201	YDGERKKTLCGTPNY
T205	RKKTLCGTPNYIAPE
Y208	TLCGTPNYIAPEVLG
G215	YIAPEVLGKKGHSFE
K263	KNEYSIPKHINPVAA
Y300	DEFFTSGYIPSRLPT
S317	LTVPPRFSIAPSTID
S321	PRFSIAPSTIDQsLR
T322	RFSIAPSTIDQsLRK
S326-p	APSTIDQsLRKPLTA
K329	TIDQsLRKPLTAINK
T332	QsLRKPLTAINKGQD
S340-p	AINKGQDsPLVEKQV
S369	EPADCYLSEMLQQLT
C377	EMLQQLTCLNAVKPS
V381	QLTCLNAVKPSERAL
S444	IMYNDGDSLQYIERN
S455	IERNNTESYLNVRSY
N458	NNTESYLNVRSYPTT
R460	TESYLNVRSYPTTLT
K468	SYPTTLTKKITLLKY
K474	TKKITLLKYFRNYMS
K486	YMSEHLLKAGANTTP
T492	LKAGANTTPREGDEL
S559	EFRTYKLSLIQEFGC
S572	GCCKELASRLRYART
K583	YARTMVEKLQSSKSA
V591	LQSSKSAVAHVKASA

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