Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
PLK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PLK1 a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.21; Kinase, protein; Other group; PLK family
Chromosomal Location of Human Ortholog: 16p12.2
Cellular Component: spindle pole; centrosome; cytosol; nucleoplasm; microtubule cytoskeleton; kinetochore; spindle microtubule; cytoplasm; nucleolus; spindle; spindle midzone; midbody; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of cell cycle; positive regulation of proteolysis; regulation of mitotic cell cycle; mitotic nuclear envelope disassembly; anaphase-promoting complex activation during mitotic cell cycle; protein ubiquitination; cytokinesis; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to antibiotic; establishment of protein localization; cytokinesis after mitosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; centrosome organization and biogenesis; positive regulation of ubiquitin-protein ligase activity; mitotic cell cycle spindle assembly checkpoint; G2/M transition of mitotic cell cycle; mitosis; mitotic sister chromatid segregation; protein destabilization; regulation of mitotic metaphase/anaphase transition; negative regulation of cyclin-dependent protein kinase activity; cell proliferation; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; polar body extrusion after meiotic divisions; mitotic cell cycle; G2/M transition DNA damage checkpoint; mitotic metaphase/anaphase transition; microtubule bundle formation; negative regulation of apoptosis
Reference #:  P53350 (UniProtKB)
Alt. Names/Synonyms: cell cycle regulated protein kinase; PLK; PLK-1; PLK1; polo (Drosophia)-like kinase; polo like kinase; Polo-like kinase 1; polo-like kinase 1 (Drosophila); Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13
Gene Symbols: PLK1
Molecular weight: 68,255 Da
Basal Isoelectric point: 9.09  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PLK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

STRING  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  KinBase  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  InnateDB


Sites Implicated In
cell cycle regulation: S49‑p, S137‑p, T210‑p, S326‑p, S330‑p, S597‑p
enzymatic activity, induced: S49‑p, S137‑p, T210‑p, S326‑p
molecular association, regulation: T210‑p, S330‑p, S597‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S2-p ______MsAAVtAGk
0 2 T6-p __MsAAVtAGkLARA
0 1 K9-ac sAAVtAGkLARAPAD
1 1 A34 APGAPAAAPPAkEIP
1 1 P35 PGAPAAAPPAkEIPE
0 2 K38-ub PAAAPPAkEIPEVLV
1 0 S49-p EVLVDPRsRRRYVRG
0 1 S103-p EKMSMEIsIHRSLAH
4 1 S137-p LELCRRRsLLELHKR
0 1 K146-ub LELHKRRkALTEPEA
0 1 K200-ub GDFGLATkVEYDGER
18 58 T210-p YDGERKKtLCGtPNy
0 15 T214-p RKKtLCGtPNyIAPE
0 148 Y217-p tLCGtPNyIAPEVLs
0 3 S224-p yIAPEVLsKKGHSFE
0 1 K272-ub KNEYSIPkHINPVAA
0 1 Y309-p DEFFTSGyIPARLPI
1 0 S326-p LTIPPRFsIAPssLD
2 0 S330-p PRFsIAPssLDPsNR
0 3 S331-p RFsIAPssLDPsNRk
1 2 S335-p APssLDPsNRkPLtV
0 1 K338-ub sLDPsNRkPLtVLNK
0 2 T341-p PsNRkPLtVLNKGLE
1 1 N349 VLNKGLENPLPERPR
0 1 T366-p EEPVVREtGEVVDCH
0 3 S375-p EVVDCHLsDMLQQLH
1 1 S383-p DMLQQLHsVNAsKPS
0 1 S387-p QLHsVNAsKPSERGL
0 2 S450-p ILYNDGDsLQYIERD
0 1 T459-p QYIERDGtEsyLtVs
0 2 S461-p IERDGtEsyLtVsSH
0 1 Y462-p ERDGtEsyLtVsSHP
0 1 T464-p DGtEsyLtVsSHPNS
0 1 S466-p tEsyLtVsSHPNSLM
0 1 K474-ub SHPNSLMkKITLLkY
0 1 K480-ub MkKITLLkYFRNYMS
0 2 K492-ub YMSEHLLkAGANItP
0 3 T498-p LkAGANItPREGDEL
0 1 S565-p DFRTYRLsLLEEYGC
0 1 S578-p GCCKELAsRLRYART
0 1 K589-ub YARTMVDkLLSSRSA
2 0 S597-p LLSSRSAsNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
9062 : Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb
  mouse

 
N2 ______MNAAAKAGK
K6 __MNAAAKAGKLARA
K9 NAAAKAGKLARAPAD
A34 VPGAPVAAPLAKEIP
P35 PGAPVAAPLAKEIPE
K38 PVAAPLAKEIPEVLV
S49 EVLVDPRSRRQYVRG
S103 EKMSMEISIHRSLAH
S137 LELCRRRSLLELHKR
K146 LELHKRRKALTEPEA
K200 GDFGLATKVEYEGER
T210-p YEGERKKtLCGtPNY
T214-p RKKtLCGtPNYIAPE
Y217 tLCGtPNYIAPEVLS
S224 YIAPEVLSKKGHSFE
K272 KNEYSIPKHINPVAA
Y309 DEFFTSGYIPARLPI
S326 LTIPPRFSIAPSsLD
S330 PRFSIAPSsLDPSSR
S331-p RFSIAPSsLDPSSRK
S335 APSsLDPSSRKPLKV
K338 sLDPSSRKPLKVLNK
K341 PSSRKPLKVLNKGVE
N349 VLNKGVENPLPDRPR
T366 EEPVVRETNEAIECH
S375 EAIECHLSDLLQQLT
S383 DLLQQLTSVNASKPS
S387 QLTSVNASKPSERGL
S450 ILYNDGDSLQYIERD
T459 QYIERDGTESYLTVS
S461 IERDGTESYLTVSSH
Y462 ERDGTESYLTVSSHP
T464 DGTESYLTVSSHPNS
S466 TESYLTVSSHPNSLM
K474 SHPNSLMKKITLLNY
N480 MKKITLLNYFRNYMS
K492 YMSEHLLKAGANITP
T498 LKAGANITPREGDEL
S565 DFQTYRLSLLEEYGC
S578 GCCKELASRLRYART
K589 YARTMVDKLLSSRSA
S597 LLSSRSASNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
  rat

 
N2 ______MNAAAKAGK
K6 __MNAAAKAGKLARA
K9 NAAAKAGKLARAPAD
A34 APGAPGAAPLAKEIP
P35 PGAPGAAPLAKEIPE
K38 PGAAPLAKEIPEVLM
S49 EVLMDPRSRRQYVRG
S103 EKMSMEISIHRSLAH
S137 LELCRRRSLLELHKR
K146 LELHKRRKALTEPEA
K200 GDFGLATKVEYEGER
T210-p YEGERKKtLCGTPNY
T214 RKKtLCGTPNYIAPE
Y217 tLCGTPNYIAPEVLS
S224 YIAPEVLSKKGHSFE
K272 KNEYSIPKHINPVAA
Y309 DEFFTSGYIPARLPI
S326 LTIPPRFSIAPSSLD
S330 PRFSIAPSSLDPSNR
S331 RFSIAPSSLDPSNRK
S335 APSSLDPSNRKPLTV
K338 SLDPSNRKPLTVLNK
T341 PSNRKPLTVLNKGVE
N349 VLNKGVENPLPDRPR
T366 EEPVVRETNEAIECH
S375 EAIECHLSDLLQQLT
S383 DLLQQLTSVNASKPS
S387 QLTSVNASKPSERGL
S450 ILYNDGDSLQYIERD
T459 QYIERDGTESYLTVS
S461 IERDGTESYLTVSSH
Y462 ERDGTESYLTVSSHP
T464 DGTESYLTVSSHPNS
S466 TESYLTVSSHPNSLM
K474 SHPNSLMKKITLLNY
N480 MKKITLLNYFRNYMS
K492 YMSEHLLKAGANITP
T498 LKAGANITPREGDEL
S565 DFRTYRLSLLEEYGC
S578 GCCKELASRLRYART
K589 YARTMVDKLLSSRSA
C597 LLSSRSACNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
  frog

 
- gap
- gap
K7 _MAQVAGKKLTVAPE
S25-p PPGIPGSssAVKEIP
S26-p PGIPGSssAVKEIPE
K29 PGSssAVKEIPEILV
T40 EILVDPRTRRRYLRG
A94 DKMTMEIAIQRSLDH
S128 LELCRRRSLLELHKR
K137 LELHKRRKAVTEPEA
K191 GDFGLATKVEYDGER
T201 YDGERKKTLCGTPNY
T205 RKKTLCGTPNYIAPE
Y208 TLCGTPNYIAPEVLG
G215 YIAPEVLGKKGHSFE
K263 KNEYSIPKHINPVAA
Y300 DEFFTSGYIPSRLPT
S317 LTVPPRFSIAPSTID
S321 PRFSIAPSTIDQsLR
T322 RFSIAPSTIDQsLRK
S326-p APSTIDQsLRKPLTA
K329 TIDQsLRKPLTAINK
T332 QsLRKPLTAINKGQD
S340-p AINKGQDsPLVEKQV
T361 EMQQPEFTEPADCYL
S369 EPADCYLSEMLQQLT
C377 EMLQQLTCLNAVKPS
V381 QLTCLNAVKPSERAL
S444 IMYNDGDSLQYIERN
T453 QYIERNNTESYLNVR
S455 IERNNTESYLNVRSY
Y456 ERNNTESYLNVRSYP
N458 NNTESYLNVRSYPTT
R460 TESYLNVRSYPTTLT
K468 SYPTTLTKKITLLKY
K474 TKKITLLKYFRNYMS
K486 YMSEHLLKAGANTTP
T492 LKAGANTTPREGDEL
S559 EFRTYKLSLIQEFGC
S572 GCCKELASRLRYART
K583 YARTMVEKLQSSKSA
V591 LQSSKSAVAHVKASA
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.