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Protein Page:
PKR (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.10.2; Translation; Kinase, protein; EC 2.7.11.1; Other group; PEK family
Chromosomal Location of Human Ortholog: 2p22-p21
Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity
Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; activation of MAPKK activity; translation; unfolded protein response; response to virus; protein amino acid autophosphorylation; viral infectious cycle; protein amino acid phosphorylation; positive regulation of chemokine production; positive regulation of stress-activated MAPK cascade; evasion by virus of host immune response; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; modification by virus of host cellular process; negative regulation of viral genome replication; negative regulation of translation; virus-host interaction; innate immune response; negative regulation of osteoblast proliferation; defense response to virus
Reference #:  P19525 (UniProtKB)
Alt. Names/Synonyms: double stranded RNA activated protein kinase; E2AK2; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; P1/eIF-2A protein kinase; p68 kinase; PKR; PRKR; Protein kinase RNA-activated; protein kinase, interferon-inducible double stranded RNA dependent
Gene Symbols: EIF2AK2
Molecular weight: 62,094 Da
Basal Isoelectric point: 8.58  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PKR

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell cycle regulation: T446‑p
cell growth, altered: Y101‑p, Y162‑p, Y293‑p, T446‑p
translation, altered: Y101‑p, Y162‑p, Y293‑p
enzymatic activity, induced: S83‑p, T88‑p, T89‑p, T90‑p, Y101‑p, Y162‑p, S242‑p, T255‑p, T258‑p, Y293‑p, T446‑p, T451‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S6-p __MAGDLsAGFFMEE
0 2 N32 LKYQELPNsGPPHDR
0 1 S33-p KYQELPNsGPPHDRR
0 1 K61-m1 EGEGRSKkEAKNAAA
0 1 K69 EAKNAAAKLAVEILN
0 1 K69-m1 EAKNAAAkLAVEILN
1 8 S83-p NKEKKAVsPLLLttt
1 0 T88-p AVsPLLLtttNsSEG
1 1 T89-p VsPLLLtttNsSEGL
1 0 T90-p sPLLLtttNsSEGLS
0 2 S92-p LLLtttNsSEGLSMG
1 0 Y101-p EGLSMGNyIGLINRI
0 2 T115 IAQKKRLTVNYEQCA
0 1 Y142-p CKMGQKEySIGTGST
1 0 Y162-p QLAAKLAyLQILSEE
0 6 - gap
0 3 S179-p VKSDYLSsGsFATTC
0 3 S181-p SDYLSsGsFATTCES
2 1 S242-p NQRKAKRsLAPRFDL
0 4 - gap
2 0 T255-p DLPDMKEtKYtVDKR
2 0 T258-p DMKEtKYtVDKRFGM
0 4 K268-ub KRFGMDFkEIELIGS
1 0 Y293-p HRIDGKTyVIKRVkY
0 1 K299-ub TyVIKRVkYNNEkAE
0 4 K304-ub RVkYNNEkAEREVKA
0 1 K385-ub IEKRRGEkLDkVLAL
0 1 K388-ub RRGEkLDkVLALELF
0 8 K400-ub ELFEQITkGVDYIHS
0 4 K408-ub GVDYIHSkKLIHRDL
0 3 K416-ub KLIHRDLkPSNIFLV
0 2 K426-ub NIFLVDTkQVkIGDF
0 4 K429-ub LVDTkQVkIGDFGLV
0 2 K440-ub FGLVTSLkNDGKRtR
7 1 T446-p LkNDGKRtRSKGtLR
4 1 T451-p KRtRSKGtLRyMsPE
0 1 Y454-p RSKGtLRyMsPEQIS
0 3 S456-p KGtLRyMsPEQISSQ
0 1 K509-ub IISDIFDkKEKTLLQ
0 2 K517-ub KEKTLLQkLLSkKPE
0 2 K521-ub LLQkLLSkKPEDRPN
0 1 T535-p NTSEILRtLTVWKKs
0 4 S542-p tLTVWKKsPEKNERH
3071 : Phospho-PKR (Thr446/451) Antibody
3071 : Phospho-PKR (Thr446/451) Antibody
  mouse

 
T5 ___MASDTPGFYMDK
T31-p ITYKELStSGPPHDR
S32 TYKELStSGPPHDRR
Q60 EAKGRSKQEARNAAA
K68-ac EARNAAAkLAVDILD
K68 EARNAAAKLAVDILD
C82 DNENKVDCHTSASEQ
H83 NENKVDCHTSASEQG
T84 ENKVDCHTSASEQGL
S85 NKVDCHTSASEQGLF
S87 VDCHTSASEQGLFVG
Y96 QGLFVGNYIGLVNSF
S110-p FAQKKKLsVNYEQCE
Y137 CKIGQTMYGTGSGVT
Y157 QLAAKEAYQKLLKsP
S163-p AYQKLLKsPPKTAGT
- gap
- gap
- gap
S223-p RKSGVKVsPDDVQRN
N230 sPDDVQRNKYTLDAR
T233 DVQRNKYTLDARFNS
E243 ARFNSDFEDIEEIGL
Y268 HRIDGKRYAIKRVKY
K274 RYAIKRVKYNTEKAE
K279 RVKYNTEKAEHEVQA
K347 MRNRNQSKVDKALIL
K350 RNQSKVDKALILDLY
T362 DLYEQIVTGVEYIHS
K370 GVEYIHSKGLIHRDL
K378-ub GLIHRDLkPGNIFLV
R388 NIFLVDERHIKIGDF
K391 LVDERHIKIGDFGLA
E402 FGLATALENDGKSRt
T409-p ENDGKSRtRRTGTLQ
T414 SRtRRTGTLQYMSPE
Y417 RRTGTLQYMSPEQLF
S419 TGTLQYMSPEQLFLK
N473 FSNDIFDNKEKSLLK
K481 KEKSLLKKLLSEKPK
E485 LLKKLLSEKPKDRPE
T499 ETSEILKTLAEWRNI
S507 LAEWRNISEKKKRNT
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