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Protein Page:
PKCG (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PKCG a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. Expressed in the brain and spinal cord where its localization is restricted to neurons. Several neuronal functions, including long term potentiation and depression (LTP&LTD) specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects have been associated with neurodegenerative disorder spinocerebellar ataxia-14. Plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GLUR4 and NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor MGLUR5 and phosphorylates NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43, resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53 and promotes p53-dependent apoptosis in response to DNA damage. Interacts with GRIA4. Interacts with CDCP1. Interacts with TP53INP1 and p53. Expressed in Purkinje cells of the cerebellar cortex. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; AGC group; PKC family; Alpha subfamily
Cellular Component: perinuclear region of cytoplasm; dendrite; plasma membrane; nucleus; cytosol; cell junction
Molecular Function: protein kinase C activity; zinc ion binding; protein serine/threonine/tyrosine kinase activity; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity
Biological Process: cell death; epidermal growth factor receptor signaling pathway; platelet activation; positive regulation of mismatch repair; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; response to morphine; response to pain; signal transduction; protein amino acid phosphorylation; synaptic transmission; learning and/or memory; phospholipase C activation; innate immune response; chemosensory behavior; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; blood coagulation; negative regulation of protein catabolic process; phosphorylation
Reference #:  P05129 (UniProtKB)
Alt. Names/Synonyms: KPCG; MGC57564; PKC-gamma; PKCC; PKCG; PRKCG; Protein kinase C gamma type; protein kinase C, gamma; SCA14
Gene Symbols: PRKCG
Molecular weight: 78,448 Da
Basal Isoelectric point: 7.27  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  Mitochondrial Control of Apoptosis  |  Phospholipase Signaling
Select Structure to View Below

PKCG

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S11 LGPGVGDSEGGPRPL
0 1 K44 KFTARFFKQPTFCSH
0 1 T47 ARFFKQPTFCSHCTD
0 2 S70 GLQCQVCSFVVHRRC
0 1 T82 RRCHEFVTFECPGAG
0 5 Y122 DHCGSLLYGLVHQGM
0 1 S145-p VHRRCVRsVPsLCGV
0 2 S148-p RCVRsVPsLCGVDHt
0 1 T155-p sLCGVDHtERRGRLQ
0 1 A170 LEIRAPTADEIHVTV
0 122 Y195-p PNGLSDPyVKLKLIP
0 2 K197 GLSDPyVKLKLIPDP
0 1 K199 SDPyVKLKLIPDPRN
0 1 K199 SDPyVKLKLIPDPRN
0 1 T208 IPDPRNLTKQKTRTV
0 1 T212 RNLTKQKTRTVKATL
0 2 K216 KQKTRTVKATLNPVW
0 6 K232 ETFVFNLKPGDVERR
0 1 S241 GDVERRLSVEVWDWD
0 6 Y275 KAPVDGWYKLLNQEE
0 11 Y285 LNQEEGEYYNVPVAD
0 10 Y286 NQEEGEYYNVPVADA
0 4 S297 VADADNCSLLQKFEA
0 5 Y307 QKFEACNYPLELYER
0 9 Y312 CNYPLELYERVRMGP
0 2 S320-p ERVRMGPsSSPIPSP
0 1 S321 RVRMGPsSSPIPSPS
0 5 S322 VRMGPsSSPIPSPSP
0 4 S326 PsSSPIPSPSPSPTD
0 1 S326 PsSSPIPSPSPSPTD
0 4 S328 SSPIPSPSPSPTDPK
0 1 S328 SSPIPSPSPSPTDPK
0 5 S330 PIPSPSPSPTDPKRC
0 1 S330 PIPSPSPSPTDPKRC
0 2 T332 PSPSPSPTDPKRCFF
0 4 K335 SPSPTDPKRCFFGAs
0 3 S342-p KRCFFGAsPGRLHIS
0 1 K359-ac SFLMVLGkGSFGKVM
0 1 K364 LGkGSFGKVMLAERR
0 4 S373 MLAERRGSDELYAIK
0 5 Y377 RRGSDELYAIKILKK
0 1 K380 SDELYAIKILKKDVI
0 1 T396 QDDDVDCTLVEKRVL
0 1 K400 VDCTLVEKRVLALGG
0 2 R479 HNQGIIYRDLKLDNV
0 1 K482 GIIYRDLKLDNVMLD
0 1 K503-ac ITDFGMCkENVFPGt
0 7 K503 ITDFGMCKENVFPGt
0 3 T510-p kENVFPGttTRtFCG
0 3 T511-p ENVFPGttTRtFCGt
0 1 T512 NVFPGttTRtFCGtP
3 53 T514-p FPGttTRtFCGtPDy
0 15 T518-p tTRtFCGtPDyIAPE
0 15 Y521-p tFCGtPDyIAPEIIA
0 10 Y529-p IAPEIIAyQPyGKSV
0 5 Y532-p EIIAyQPyGKSVDWW
0 1 K587 REAVAICKGFLTKHP
0 4 K587 REAVAICKGFLTKHP
0 1 T591 AICKGFLTKHPGKRL
0 6 K592 ICKGFLTKHPGKRLG
0 2 K596 FLTKHPGKRLGSGPD
0 1 S639 RPRPCGRSGENFDKF
0 1 S639 RPRPCGRSGENFDKF
0 4 K645 RSGENFDKFFTRAAP
2 14 T655-p TRAAPALtPPDRLVL
3 3 T674-p QADFQGFtYVNPDFV
0 1 Y675 ADFQGFtYVNPDFVH
0 6 S687 FVHPDARSPTSPVPV
0 2 T689 HPDARSPTSPVPVPV
0 1 T689 HPDARSPTSPVPVPV
0 3 S690 PDARSPTSPVPVPVM
0 1 S690 PDARSPTSPVPVPVM
2060 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb
5016 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb (Biotinylated)
9379 : Phospho-PKC (pan) (gamma Thr514) Antibody
  mouse

 
S11-p LGPGGGDsEGGPRPL
K44 KFTARFFKQPtFCSH
T47-p ARFFKQPtFCSHCTD
S70-p GLQCQVCsFVVHRRC
T82 RRCHEFVTFECPGAG
Y122 DHCGSLLYGLVHQGM
S145 VHRRCVRSVPsLCGV
S148-p RCVRSVPsLCGVDHT
T155 sLCGVDHTERRGRLQ
S170-p LEIRAPTsDEIHITV
Y195-p PNGLSDPyVkLkLIP
K197-ub GLSDPyVkLkLIPDP
K199 SDPyVkLKLIPDPRN
K199-ub SDPyVkLkLIPDPRN
T208 IPDPRNLTKQKTKTV
T212 RNLTKQKTKTVKATL
K216 KQKTKTVKATLNPVW
K232 ETFVFNLKPGDVERR
S241 GDVERRLSVEVWDWD
Y275 KAPVDGWYKLLNQEE
Y285 LNQEEGEYYNVPVAD
Y286 NQEEGEYYNVPVADA
S297-p VADADNCsLLQKFEA
Y307 QKFEACNYPLELyER
Y312-p CNYPLELyERVRMGP
S320-p ERVRMGPsSsPIPsP
S321 RVRMGPsSsPIPsPs
S322-p VRMGPsSsPIPsPsP
S326-p PsSsPIPsPsPsPtD
S326 PsSsPIPSPsPsPtD
S328-p SsPIPsPsPsPtDSk
S328 SsPIPsPSPsPtDSk
S330-p PIPsPsPsPtDSkRC
S330 PIPsPsPSPtDSkRC
T332-p PsPsPsPtDSkRCFF
K335-ub sPsPtDSkRCFFGAs
S342-p kRCFFGAsPGRLHIS
K359 SFLMVLGKGSFGkVM
K364-ub LGKGSFGkVMLAERR
S373-p MLAERRGsDELYAIk
Y377 RRGsDELYAIkILKK
K380-ac sDELYAIkILKKDVI
T396 QDDDVDCTLVEKRVL
K400 VDCTLVEKRVLALGG
R479-m1 HNQGIIYrDLKLDNV
K482 GIIYrDLKLDNVMLD
K503 ITDFGMCKENVFPGS
K503-ub ITDFGMCkENVFPGS
S510 kENVFPGSttRtFCG
T511-p ENVFPGSttRtFCGt
T512-p NVFPGSttRtFCGtP
T514-p FPGSttRtFCGtPDy
T518-p ttRtFCGtPDyIAPE
Y521-p tFCGtPDyIAPEIIA
Y529 IAPEIIAYQPYGKSV
Y532 EIIAYQPYGKSVDWW
K587 REAVAICKGFLTkHP
K587-ub REAVAICkGFLTkHP
T591 AICkGFLTkHPGKRL
K592-ub ICkGFLTkHPGKRLG
K596 FLTkHPGKRLGSGPD
S639-p RPRPCGRsGENFDkF
S639 RPRPCGRSGENFDkF
K645-ub RsGENFDkFFTRAAP
T655-p TRAAPALtPPDRLVL
T674-p QADFQGFtyVNPDFV
Y675-p ADFQGFtyVNPDFVH
S687-p FVHPDARsPtsPVPV
T689-p HPDARsPtsPVPVPV
T689 HPDARsPTsPVPVPV
S690-p PDARsPtsPVPVPVM
S690 PDARsPtSPVPVPVM
2060 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb
5016 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb (Biotinylated)
9379 : Phospho-PKC (pan) (gamma Thr514) Antibody
  rat

 
S11 LGPGGGDSEGGPRPL
K44-ub KFTARFFkQPTFCSH
T47 ARFFkQPTFCSHCTD
S70 GLQCQVCSFVVHRRC
T82-gl RRCHEFVtFECPGAG
Y122-p DHCGSLLyGLVHQGM
S145 VHRRCVRSVPSLCGV
S148 RCVRSVPSLCGVDHT
T155 SLCGVDHTERRGRLQ
S170 LEIRAPTSDEIHITV
Y195-p PNGLSDPyVkLkLIP
K197-ub GLSDPyVkLkLIPDP
K199-ac SDPyVkLkLIPDPRN
K199 SDPyVkLKLIPDPRN
T208-p IPDPRNLtKQKtKTV
T212-p RNLtKQKtKTVkATL
K216-ub KQKtKTVkATLNPVW
K232-ub ETFVFNLkPGDVERR
S241-p GDVERRLsVEVWDWD
Y275-p KAPVDGWyKLLNQEE
Y285-p LNQEEGEyyNVPVAD
Y286-p NQEEGEyyNVPVADA
S297-p VADADNCsLLQKFEA
Y307-p QKFEACNyPLELyER
Y312-p CNyPLELyERVRMGP
S320 ERVRMGPSsSPIPsP
S321-gl RVRMGPSsSPIPsPs
S322 VRMGPSsSPIPsPsP
S326 PSsSPIPSPsPsPTD
S326-gl PSsSPIPsPsPsPTD
S328 sSPIPsPSPsPTDSk
S328-gl sSPIPsPsPsPTDSk
S330 PIPsPsPSPTDSkRC
S330-gl PIPsPsPsPTDSkRC
T332 PsPsPsPTDSkRCFF
K335-ub sPsPTDSkRCFFGAS
S342 kRCFFGASPGRLHIS
K359 SFLMVLGKGSFGKVM
K364 LGKGSFGKVMLAERR
S373-p MLAERRGsDELyAIK
Y377-p RRGsDELyAIKILKK
K380 sDELyAIKILKKDVI
T396-gl QDDDVDCtLVEkRVL
K400-ub VDCtLVEkRVLALGG
R479 HNQGIIYRDLkLDNV
K482-ub GIIYRDLkLDNVMLD
K503 ITDFGMCKENVFPGS
K503-ub ITDFGMCkENVFPGS
S510 kENVFPGSTTRtFCG
T511 ENVFPGSTTRtFCGt
T512 NVFPGSTTRtFCGtP
T514-p FPGSTTRtFCGtPDy
T518-p TTRtFCGtPDyIAPE
Y521-p tFCGtPDyIAPEIIA
Y529-p IAPEIIAyQPyGKSV
Y532-p EIIAyQPyGKSVDWW
K587-ac REAVAICkGFLtkHP
K587-ub REAVAICkGFLtkHP
T591-gl AICkGFLtkHPGkRL
K592-ub ICkGFLtkHPGkRLG
K596-ub FLtkHPGkRLGSGPD
S639 RPRPCGRSGENFDkF
S639-gl RPRPCGRsGENFDkF
K645-ub RsGENFDkFFTRAAP
T655-p TRAAPALtPPDRLVL
T674-p QADFQGFtYVNPDFV
Y675 ADFQGFtYVNPDFVH
S687 FVHPDARSPtsPVPV
T689 HPDARSPTsPVPVPV
T689-gl HPDARSPtsPVPVPV
S690 PDARSPtSPVPVPVM
S690-gl PDARSPtsPVPVPVM
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