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Protein Page:
LEDGF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LEDGF Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN. Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. Belongs to the HDGF family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Transcription, coactivator/corepressor
Cellular Component: nucleoplasm; nucleolus; nuclear heterochromatin; nucleus; cytosol
Molecular Function: protein binding; transcription activator binding; chromatin binding
Biological Process: nuclear mRNA 5'-splice site recognition; viral reproduction; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to heat; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress
Reference #:  Q9UER6 (UniProtKB)
Alt. Names/Synonyms: CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; DFS70; LEDGF; Lens epithelium-derived growth factor; MGC74712; p52; p75; PAIP; PC4 and SFRS1 interacting protein 1; PC4 and SFRS1-interacting protein; PSIP1; PSIP2; transcriptional coactivator p52/p75; Transcriptional coactivator p75/p52
Gene Symbols: PSIP1
Molecular weight: 60,103 Da
Basal Isoelectric point: 9.15  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

LEDGF

Protein Structure Not Found.


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Sites Implicated In
phosphorylation: S206‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 K6-ub __MTRDFkPGDLIFA
0 1 K14-ub PGDLIFAkMkGYPHW
0 1 K16-ub DLIFAkMkGYPHWPA
0 2 K34-ub EVPDGAVkPPTNKLP
0 1 K56 ETAFLGPKDIFPYsE
0 2 S62-p PKDIFPYsENKEKYG
0 1 K65 IFPYsENKEKYGKPN
0 1 K65 IFPYsENKEKYGKPN
1 0 K75-sm YGKPNKRkGFNEGLW
0 1 K75-ub YGKPNKRkGFNEGLW
0 6 S94-p NPKVKFSsQQAATkQ
0 5 K100-ub SsQQAATkQsNAssD
0 9 S102-p QQAATkQsNAssDVE
0 9 S105-p ATkQsNAssDVEVEE
0 39 S106-p TkQsNAssDVEVEEK
0 6 T115-p VEVEEKEtsVsKEDt
0 1 S116-p EVEEKEtsVsKEDtD
0 10 S118-p EEKEtsVsKEDtDHE
0 35 T122-p tsVsKEDtDHEEKAs
0 30 S129-p tDHEEKAsNEDVTkA
0 3 T134 KAsNEDVTkAVDItt
0 2 K135-ub AsNEDVTkAVDIttP
0 2 T140-p VTkAVDIttPKAARR
0 20 T141-p TkAVDIttPKAARRG
0 2 T159-p KAEKQVEtEEAGVVt
0 3 T166-p tEEAGVVttAtAsVN
0 2 T167-p EEAGVVttAtAsVNL
0 5 T169-p AGVVttAtAsVNLKV
0 10 S171-p VVttAtAsVNLKVsP
0 52 S177-p AsVNLKVsPKRGRPA
0 4 T186-p KRGRPAAtEVKIPKP
1 5 S206-p MVKQPCPsESDIITE
0 3 K220-ac EEDKSKKkGQEEkQP
0 3 K225-ac KKkGQEEkQPkkQPk
0 2 K228-ac GQEEkQPkkQPkKDE
0 1 K229-ac QEEkQPkkQPkKDEE
0 1 K232-ac kQPkkQPkKDEEGQK
1 0 K250-sm KPRKEPDkKEGkKEV
1 0 K254-sm EPDkKEGkKEVESKR
0 2 T267-p KRKNLAKtGVtstsD
0 15 T270-p NLAKtGVtstsDsEE
1 24 S271-p LAKtGVtstsDsEEE
0 25 T272-p AKtGVtstsDsEEEG
1 102 S273-p KtGVtstsDsEEEGD
1 120 S275-p GVtstsDsEEEGDDQ
0 1 K304 AHRRNMLKGQHEKEA
0 1 T324-p KQEEQMEtEQQNKDE
0 2 T346-p KVEKKREtsMDsRLQ
0 1 S347-p VEKKREtsMDsRLQR
0 1 S350-p KREtsMDsRLQRIHA
2 0 K364-sm AEIKNSLkIDNLDVN
0 3 K364-ub AEIKNSLkIDNLDVN
0 1 K407-ub LKKIRRFkVSQVIME
0 1 K415-ub VSQVIMEkSTMLYNk
0 1 K422-ub kSTMLYNkFkNMFLV
0 2 K424-ub TMLYNkFkNMFLVGE
0 4 S434-p FLVGEGDsVItQVLN
0 3 T437-p GEGDsVItQVLNksL
0 8 K442-ub VItQVLNksLAEQRQ
0 7 S443-p ItQVLNksLAEQRQH
0 3 K461-ac NKTKDQGkkGPNkKL
0 4 K462-ac KTKDQGkkGPNkKLE
0 1 K466-ac QGkkGPNkKLEKEQT
0 1 S496-p QPQHNGEsNEDSKDN
0 1 S513-p ASTKKKPssEEREtE
0 3 S514-p STKKKPssEEREtEI
0 3 T519-p PssEEREtEIsLkDs
0 6 S522-p EEREtEIsLkDstLD
0 6 K524-ub REtEIsLkDstLDN_
0 1 S526-p tEIsLkDstLDN___
0 5 T527-p EIsLkDstLDN____
  LEDGF iso2  
K6 __MTRDFKPGDLIFA
K14 PGDLIFAKMKGYPHW
K16 DLIFAKMKGYPHWPA
K34 EVPDGAVKPPTNKLP
K56 ETAFLGPKDIFPYSE
S62 PKDIFPYSENKEKYG
K65 IFPYSENKEKYGKPN
K65 IFPYSENKEKYGKPN
K75-sm YGKPNKRkGFNEGLW
K75 YGKPNKRKGFNEGLW
S94 NPKVKFSSQQAATKQ
K100 SSQQAATKQSNASSD
S102 QQAATKQSNASSDVE
S105 ATKQSNASSDVEVEE
S106 TKQSNASSDVEVEEK
T115 VEVEEKETSVSKEDT
S116 EVEEKETSVSKEDTD
S118 EEKETSVSKEDTDHE
T122 TSVSKEDTDHEEKAS
S129 TDHEEKASNEDVTKA
T134 KASNEDVTKAVDITT
K135 ASNEDVTKAVDITTP
T140 VTKAVDITTPKAARR
T141 TKAVDITTPKAARRG
T159 KAEKQVETEEAGVVT
T166 TEEAGVVTTATASVN
T167 EEAGVVTTATASVNL
T169 AGVVTTATASVNLKV
S171 VVTTATASVNLKVSP
S177 ASVNLKVSPKRGRPA
T186 KRGRPAATEVKIPKP
S206 MVKQPCPSESDIITE
K220 EEDKSKKKGQEEKQP
K225 KKKGQEEKQPKKQPK
K228 GQEEKQPKKQPKKDE
K229 QEEKQPKKQPKKDEE
K232 KQPKKQPKKDEEGQK
K250-sm KPRKEPDkKEGkKEV
K254-sm EPDkKEGkKEVESKR
T267 KRKNLAKTGVTSTSD
T270 NLAKTGVTSTSDSEE
S271 LAKTGVTSTSDSEEE
T272 AKTGVTSTSDSEEEG
S273 KTGVTSTSDSEEEGD
S275 GVTSTSDSEEEGDDQ
K304 AHRRNMLKGQHEKEA
T324 KQEEQMETEHQTTCN
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
K6 __MTRDFKPGDLIFA
K14 PGDLIFAKMKGYPHW
K16 DLIFAKMKGYPHWPA
K34 EVPDGAVKPPTNKLP
K56-ac ETAFLGPkDIFPYSE
S62 PkDIFPYSENkEKYG
K65-ac IFPYSENkEKYGKPN
K65-ub IFPYSENkEKYGKPN
K75 YGKPNKRKGFNEGLW
K75 YGKPNKRKGFNEGLW
S94-p NPKVKFSsQQASTKQ
K100 SsQQASTKQsNAssD
S102-p QQASTKQsNAssDVE
S105-p STKQsNAssDVEVEE
S106-p TKQsNAssDVEVEEK
T115-p VEVEEKEtNVsKEDt
N116 EVEEKEtNVsKEDtD
S118-p EEKEtNVsKEDtDQE
T122-p tNVsKEDtDQEEKAs
S129-p tDQEEKAsNEDVtKA
T134-p KAsNEDVtKAVDItt
K135 AsNEDVtKAVDIttP
T140-p VtKAVDIttPKAARR
T141-p tKAVDIttPKAARRG
T159-p KAEKQVDtEEAGMVt
T166-p tEEAGMVtAAtAsNV
A167 EEAGMVtAAtAsNVK
T169-p AGMVtAAtAsNVKAs
S171-p MVtAAtAsNVKAsPK
S176-p tAsNVKAsPKRGRPA
T185 KRGRPAATEVKIPKP
S205-p VVKQPCPsDGDMVID
K219 DEDKSKKKGPEEKQP
K224 KKKGPEEKQPKKQLK
K227 GPEEKQPKKQLKKEE
K228 PEEKQPKKQLKKEEE
K231 KQPKKQLKKEEEGQK
K249 KPRKEPDKKEGKKEV
K253 EPDKKEGKKEVESKR
P266 KRKNLAKPGVtstsD
T269-p NLAKPGVtstsDsED
S270-p LAKPGVtstsDsEDE
T271-p AKPGVtstsDsEDED
S272-p KPGVtstsDsEDEDD
S274-p GVtstsDsEDEDDQE
K302-ub AHRRNMLkGQHEKEA
T322 KQEEQMETEQQNKDE
T344-p KVEKKREtSMDSRLQ
S345 VEKKREtSMDSRLQR
S348 KREtSMDSRLQRIHA
K362 AEIKNSLKIDNLDVN
K362-ub AEIKNSLkIDNLDVN
K405 LKKIRRFKVSQVIME
K413 VSQVIMEKSTMLYNK
K420 KSTMLYNKFkNMFLV
K422-ub TMLYNKFkNMFLVGE
S432 FLVGEGDSVItQVLN
T435-p GEGDSVItQVLNksL
K440-ub VItQVLNksLAEQRQ
S441-p ItQVLNksLAEQRQH
K459 NKTKDQGKKGPNKKL
K460 KTKDQGKKGPNKKLE
K464 QGKKGPNKKLEKEPT
S494 HPQHNGDSNEDGKDS
P511 ASSKTKPPGEEREAE
G512 SSKTKPPGEEREAEI
A517 PPGEEREAEIsLkES
S520-p EEREAEIsLkESTLD
K522-ub REAEIsLkESTLDN_
S524 AEIsLkESTLDN___
T525 EIsLkESTLDN____
  rat

 
K6 __MTRDFKPGDLIFA
K14 PGDLIFAKMKGYPHW
K16 DLIFAKMKGYPHWPA
K34 EVPDGAVKPPTNKLP
K56 ETAFLGPKDIFPYSE
S62 PKDIFPYSENKEKYG
K65 IFPYSENKEKYGKPN
K65 IFPYSENKEKYGKPN
K75 YGKPNKRKGFNEGLW
K75 YGKPNKRKGFNEGLW
S94 NPKVKFSSQQVSTKQ
K100 SSQQVSTKQsNAssD
S102-p QQVSTKQsNAssDVE
S105-p STKQsNAssDVEAEE
S106-p TKQsNAssDVEAEEK
T115 VEAEEKETSVSKEDt
S116 EAEEKETSVSKEDtD
S118 EEKETSVSKEDtDQE
T122-p TSVSKEDtDQEEKAS
S129 tDQEEKASNEDVTKA
T134 KASNEDVTKAADITt
K135 ASNEDVTKAADITtP
T140 VTKAADITtPKAARR
T141-p TKAADITtPKAARRG
T159-p KAEKQVDtEEAGVVT
T166 tEEAGVVTAATASNV
A167 EEAGVVTAATASNVK
T169 AGVVTAATASNVKAs
S171 VVTAATASNVKAsPK
S176-p TASNVKAsPKRGRPA
T185 KRGRPAATEVKIPKP
S205 VVKQPCPSESDMVID
K219 DEDKSKKKGPEEKPP
K224 KKKGPEEKPPKKQLK
K227 GPEEKPPKKQLKKEE
K228 PEEKPPKKQLKKEEE
K231 KPPKKQLKKEEEGQK
K249 KPRKEPDKKEGKKEV
K253 EPDKKEGKKEVESKR
P266 KRKNLAKPGVTSTsD
T269 NLAKPGVTSTsDsEE
S270 LAKPGVTSTsDsEED
T271 AKPGVTSTsDsEEDD
S272-p KPGVTSTsDsEEDDD
S274-p GVTSTsDsEEDDDQE
K302 AHRRNMLKGQHEKEA
T322 KQEEQMETEQQTKDE
T344 KVEKKRETSMDSRLQ
S345 VEKKRETSMDSRLQR
S348 KRETSMDSRLQRIHA
K362 AEIKNSLKIDNLDVN
K362 AEIKNSLKIDNLDVN
K405 LKKIRRFKVSQVIME
K413 VSQVIMEKSTMLYNK
K420 KSTMLYNKFKNMFLV
K422 TMLYNKFKNMFLVGE
S432 FLVGEGDSVITQVLN
T435 GEGDSVITQVLNKSL
K440 VITQVLNKSLAEQRQ
S441 ITQVLNKSLAEQRQH
K459 NKTKDQGKKGPNKKL
K460 KTKDQGKKGPNKKLE
K464 QGKKGPNKKLEKEQT
S494 HPQHNGDSAEESKDS
P511 AGSKTKTPGEEREAE
G512 GSKTKTPGEEREAEV
A517 TPGEEREAEVSLKES
S520 EEREAEVSLKESTLD
K522 REAEVSLKESTLDN_
S524 AEVSLKESTLDN___
T525 EVSLKESTLDN____
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