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Protein Page:
CHIP (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CHIP E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Homodimer. Interacts with BAG2, and with the E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Interacts with the C-terminal domains of HSPA8 and HSPA1A. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1. Interacts with MKKS. Interacts with DYX1C1 and POLB. Interacts (via TPR repeats) with HSP90AA1. Interacts (when monoubiquitinated) with ATXN3. Interacts with UBE2W. Interacts (via the U-box domain) with the UBE2V2- UBE2N heterodimer; the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with DNAJB6. Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase; Adaptor/scaffold; EC 6.3.2.19
Cellular Component: intermediate filament cytoskeleton; ubiquitin conjugating enzyme complex; cytoplasm; plasma membrane; nucleolus; cytosol; nucleus; ubiquitin ligase complex; nuclear inclusion body
Molecular Function: protein binding, bridging; protein binding; enzyme binding; protein homodimerization activity; TPR domain binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; Hsp70 protein binding; misfolded protein binding; Hsp90 protein binding; SMAD binding; kinase binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; positive regulation of protein ubiquitination; protein maturation; transforming growth factor beta receptor signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; misfolded or incompletely synthesized protein catabolic process; regulation of glucocorticoid metabolic process; DNA repair; ubiquitin-dependent SMAD protein catabolic process; negative regulation of transforming growth factor beta receptor signaling pathway
Reference #:  Q9UNE7 (UniProtKB)
Alt. Names/Synonyms: Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U box-containing protein 1; STIP1 homology and U-box containing protein 1; STUB1; UBOX1
Gene Symbols: STUB1
Molecular weight: 34,856 Da
Basal Isoelectric point: 5.61  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CHIP

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 6 A15 EGGARLGAGGGsPEk
0 59 S19-p RLGAGGGsPEksPsA
0 4 K22-ub AGGGsPEksPsAQEL
0 40 S23-p GGGsPEksPsAQELK
0 7 S25-p GsPEksPsAQELKEQ
0 2 Y49-p YPEAAACyGRAITRN
0 2 S149-p AKKKRWNsIEERRIH
0 1 Y207-p IEAKHDKyMADMDEL
0 1 T271-p VGHFDPVtRsPLtQE
0 7 S273-p HFDPVtRsPLtQEQL
0 4 T276-p PVtRsPLtQEQLIPN
  mouse

 
T15-p EGGARLGtGGGGsPD
S20-p LGtGGGGsPDksPsA
K23-ub GGGGsPDksPsAQEL
S24-p GGGsPDksPsAQELK
S26-p GsPDksPsAQELKEQ
Y50 YPEAAACYGRAITRN
S150-p AKKKRWNsIEERRIH
Y208 IEAKHDKYMADMDEL
T272 VGHFDPVTRsPLtQE
S274-p HFDPVTRsPLtQEQL
T277-p PVTRsPLtQEQLIPN
  rat

 
T15-p EGGARLGtGGGGsPD
S20-p LGtGGGGsPDKsPSA
K23 GGGGsPDKsPSAQEL
S24-p GGGsPDKsPSAQELK
S26 GsPDKsPSAQELKEQ
Y50 YPEAAACYGRAITRN
S150 AKKKRWNSIEERRIH
Y208 IEAKHDKYMADMNEL
T272 VGHFDPVTRSPLTQE
S274 HFDPVTRSPLTQEQL
T277 PVTRSPLTQEQLIPN
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