Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
PAK4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PAK4 a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; PAKB subfamily
Cellular Component: Golgi apparatus
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: cell proliferation; cell migration; apoptosis; cytoskeleton organization and biogenesis; signal transduction; cell growth; cell cycle; cell motility; protein amino acid phosphorylation
Reference #:  O96013 (UniProtKB)
Alt. Names/Synonyms: KIAA1142; p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; PAK-4; PAK4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4
Gene Symbols: PAK4
Molecular weight: 64,072 Da
Basal Isoelectric point: 9.76  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PAK4

Protein Structure Not Found.


STRING  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  KinBase  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
cell growth, altered: S474‑p
enzymatic activity, induced: S474‑p
intracellular localization: S474‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K31-ub GFDQHEQkFTGLPRQ
0 8 S41-p GLPRQWQsLIEESAR
0 1 K51 EESARRPKPLVDPAC
0 1 K78-m1 VRGSKGAkDGALTLL
1 6 S99-p MSVTRSNsLRRDsPP
0 21 S104-p SNsLRRDsPPPPARA
0 3 S142-p RGRFAGHsEAGGGsG
0 9 S148-p HsEAGGGsGDRRRAG
0 4 S167-p PKSSREGsGGPQESS
0 29 S181-p SRDKRPLsGPDVGtP
0 10 T187-p LsGPDVGtPQPAGLA
0 2 S195-p PQPAGLAsGAKLAAG
0 48 T207-p AAGRPFNtyPRADTD
0 9 Y208-p AGRPFNtyPRADTDH
0 7 S258-p TRARGAPsPGVLGPH
0 10 S267-p GVLGPHAsEPQLAPP
0 2 T277-p QLAPPACtPAAPAVP
0 17 S291-p PGPPGPRsPQREPQR
0 1 S445-p HRDIKSDsILLTHDG
0 1 K455-ac LTHDGRVkLSDFGFC
0 1 K467-ac GFCAQVSkEVPRRKs
5 157 S474-p kEVPRRKsLVGtPYW
0 23 T478-p RRKsLVGtPYWMAPE
0 2 S488-p WMAPELIsRLPYGPE
0 1 K546 HKVSPSLKGFLDRLL
3241 : Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody
  mouse

 
K31 GFDQHEQKFTGLPRQ
S41 GLPRQWQSLIEESAR
K51-ub EESARRPkPLIDPAC
K78 VRGSKGAKDGALTLL
S99-p MSVTRSNsLRREsPP
S104-p SNsLRREsPPPPARA
S142 RARATGHSEAGSGSG
S148 HSEAGSGSGDRRRVG
P167 PKSSRDGPGGPQEAS
S181-p SRDKRPLsGPDVStP
T187-p LsGPDVStPQPGSLT
S195 PQPGSLTSGTKLAAG
T207-p AAGRPFNtYPRADTD
Y208 AGRPFNtYPRADTDH
S257-p TRARGAPsPGVLGPH
S266-p GVLGPHAsEPQLAPP
A276 QLAPPARALAAPAVP
S293-p PGPPGPRsPQREPQR
S447 HRDIKSDSILLTHDG
K457 LTHDGRVKLSDFGFC
K469 GFCAQVSKEVPRRKs
S476-p KEVPRRKsLVGtPYW
T480-p RRKsLVGtPYWMAPE
S490 WMAPELISRLPYGPE
K548-ub HKASPSLkGFLDRLL
  rat

 
K31 GFDQHEQKFTGLPRQ
S41 GLPRQWQSLIEESAR
K51 EESARRPKPLIDPAC
K78 VRGSKGAKDGALTLL
S99-p MSVTRSNsLRREsPP
S104-p SNsLRREsPPPPARA
S142 RGRATGHSEAGSGSG
S148 HSEAGSGSGDRRRVG
P167 PKSSREGPGGPQEVS
S181-p SRDKRPLsGPDVSTP
T187 LsGPDVSTPQPGSLA
S195 PQPGSLASETKLAAG
T207 AAGRPFNTYPRADTD
Y208 AGRPFNTYPRADTDH
S257 TRARGAPSPGVLGPH
S266 GVLGPHASEPQLAPP
A276 QLAPPARALAAPAVP
S293 PGPPGPRSPQREPQR
S447 HRDIKSDSILLTHDG
K457 LTHDGRVKLSDFGFC
K469 GFCAQVSKEVPRRKs
S476-p KEVPRRKsLVGTPYW
T480 RRKsLVGTPYWMAPE
S490 WMAPELISRLPYGPE
K548 HKASPSLKGFLDRLL
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.