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Protein Page:
RNF20 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
RNF20 Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Belongs to the BRE1 family. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: nucleolus; nucleus; ubiquitin ligase complex
Molecular Function: protein binding; zinc ion binding; histone binding; p53 binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; chromatin binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; histone monoubiquitination; positive regulation of histone methylation; protein polyubiquitination; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone H2B ubiquitination; negative regulation of cell migration
Reference #:  Q5VTR2 (UniProtKB)
Alt. Names/Synonyms: BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1-A; BRE1A; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; hBRE1; homolog of S. cerevisiae BRE1; KIAA2779; MGC129667; MGC129668; RING finger protein 20; RNF20
Gene Symbols: RNF20
Molecular weight: 113,662 Da
Basal Isoelectric point: 5.73  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

RNF20

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K21 GTSMPPEKKAAVEDS
0 1 T34-p DSGTTVEtIKLGGVs
0 1 S41-p tIKLGGVssTEELDI
0 1 S42-p IKLGGVssTEELDIR
0 1 T43 KLGGVssTEELDIRT
0 2 T122-p QGLGDLLtERKALVV
0 23 S136-p VPEPEPDsDsNQERK
0 46 S138-p EPEPDsDsNQERKDD
1 0 S172-p SSSEEMEsQLQERVE
0 1 K242-ub LTDLLQEkHRTMSQE
0 1 S262-p SKVETAEsRVsVLEs
0 1 S265-p ETAEsRVsVLEsMID
0 1 S269-p sRVsVLEsMIDDLQW
0 1 Y314-p YGAGSSLyGGTITIN
0 2 K348-ac NRLCELEkLRQDFEE
0 1 K376-ub SAVEQVVkETPEYRC
0 1 T407 AHLDEARTLLHGTRG
0 1 S515-p LNKTRLRsGsALLQs
0 1 S517-p KTRLRsGsALLQsQs
1 8 S522-p sGsALLQsQsstEDP
0 5 S524-p sALLQsQsstEDPKD
0 6 S525-p ALLQsQsstEDPKDE
0 2 T526-p LLQsQsstEDPKDEP
0 1 A534 EDPKDEPAELKPDsE
0 7 S540-p PAELKPDsEDLSsQS
1 2 S545-p PDsEDLSsQSsAsKA
0 1 S547 sEDLSsQSsAsKAsQ
0 3 S548-p EDLSsQSsAsKAsQE
0 1 S550-p LSsQSsAsKAsQEDA
1 1 S553-p QSsAsKAsQEDANEI
0 1 K610-m1 ERDSAKDkEKGkHDD
0 1 K612 DSAKDkEKGkHDDGR
0 1 K614-m1 AKDkEKGkHDDGRKK
0 2 Y650-p MKLLLDMyRSAPKEQ
0 1 K769-m1 KLMSERIkSNQIHKL
0 1 K808-ac VVRKLEEkEHLLQSN
0 1 K821-ub SNIGTGEkELGLRTQ
0 1 K857 QLELAQKKLHDFQDE
0 1 K874-ac ENSVTKEkDMFNFkR
0 1 K880-ac EkDMFNFkRAQEDIS
  mouse

 
K21-ub GTSMPPEkKTAVEDS
T34 DSGTTVETIKLGGVS
S41 TIKLGGVSStEELDI
S42 IKLGGVSStEELDIR
T43-p KLGGVSStEELDIRT
T122 QGLGDLLTERKALVV
S136-p VPEPEPDsDsNQERK
S138-p EPEPDsDsNQERKDD
S172 SSSEEMESQLQERVE
K242 LTDLLQEKHHTMSQE
S262 GKVETAESRVSVLES
S265 ETAESRVSVLESMID
S269 SRVSVLESMIDDLQW
Y314 YGAGSSLYGGTITIN
K348 NRHCELEKLRQDFEE
K376 SAVEEVVKETPEYRC
T407 AHLDEARTLLHGTRG
S515 LNKTRLRSGSALLQs
S517 KTRLRSGSALLQsQs
S522-p SGSALLQsQsstEDP
S524-p SALLQsQsstEDPKD
S525-p ALLQsQsstEDPKDE
T526-p LLQsQsstEDPKDEP
T534-p EDPKDEPtELKQDsE
S540-p PtELKQDsEDLATHS
T545 QDsEDLATHSSALKA
S547 sEDLATHSSALKASQ
S548 EDLATHSSALKASQE
L550 LATHSSALKASQEDE
S553 HSSALKASQEDEVKS
K608 ERDSVKDKEkGKHDD
K610-ub DSVKDKEkGKHDDGR
K612 VKDKEkGKHDDGRKK
Y648 MKLLLDMYRSAPKEQ
K767 KLMSERIKSNQIHKL
K806 VVRKLEEKEHLLQSN
K819 SNIGTGEKELGLRTQ
K855-ub QLELAQKkLHDFQDE
K872 ENSVTKEKDLFNFKR
K878 EKDLFNFKRAQEDIS
  rat

 
K21 GTSVPPEKKTAVEDS
T34 DSGTTVETIKLGGVS
S41 TIKLGGVSSTEELDI
S42 IKLGGVSSTEELDIR
T43 KLGGVSSTEELDIRT
T122 QGLGDLLTERKALVV
S136 VPEPEPDSDsNQERK
S138-p EPEPDSDsNQERKDD
S172 SSSEEMESQLQERVE
K242 LTDLLQEKHHTMSQE
S262 GKVETAESRVSVLES
S265 ETAESRVSVLESMID
S269 SRVSVLESMIDDLQW
Y314 YGAGSSLYGGTITIN
K348 NRHCELEKLRQDFEE
K376 SAVEEVVKETPEYRC
T407-p AHLDEARtLLHGTRG
S515 LNKTRLRSGSALLQs
S517 KTRLRSGSALLQsQs
S522-p SGSALLQsQssTEDA
S524-p SALLQsQssTEDAKD
S525-p ALLQsQssTEDAKDE
T526 LLQsQssTEDAKDEP
T534 EDAKDEPTELKQDsE
S540-p PTELKQDsEDLATHs
T545 QDsEDLATHssALKA
S547-p sEDLATHssALKASQ
S548-p EDLATHssALKASQE
L550 LATHssALKASQEDE
S553 HssALKASQEDEVKS
K608 ERDPVKDKDKGKHDD
K610 DPVKDKDKGKHDDGR
K612 VKDKDKGKHDDGRKK
Y648 MKLLLDMYRSAPKEQ
K767 KLMSERIKSNQIHKL
K806 VVRKLEEKEHLLQSN
K819 SNIGTGEKELGLRTQ
K855 QLELAQKKLHDFQDE
K872 ENSVTKEKDLFNFKR
K878 EKDLFNFKRAQEDIS
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