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RNF20 (human)

Overview RNF20 Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Belongs to the BRE1 family. Note: This description may include information from UniProtKB. Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.- Cellular Component: nucleolus; nucleus; ubiquitin ligase complex Molecular Function: protein binding; zinc ion binding; histone binding; p53 binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity Biological Process: ubiquitin-dependent protein catabolic process; histone monoubiquitination; protein polyubiquitination; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone H2B ubiquitination; negative regulation of cell migration Reference #:  Q5VTR2 (UniProtKB) Alt. Names/Synonyms: BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1-A; BRE1A; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; hBRE1; homolog of S. cerevisiae BRE1; KIAA2779; MGC129667; MGC129668; RING finger protein 20; RNF20 Gene Symbols: RNF20 Molecular weight: 113,662 Da Basal Isoelectric point: 5.73  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below RNF20

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	Modification Sites and Domains	Show Modification Legend
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	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		K21	GTSMPPEKKAAVEDS
0	1		T34-p	DSGTTVEtIKLGGVS
0	1		T43	KLGGVSSTEELDIRT
0	2		T122-p	QGLGDLLtERKALVV
0	12		S136-p	VPEPEPDsDsNQERK
0	28		S138-p	EPEPDsDsNQERKDD
1	0		S172-p	SSSEEMEsQLQERVE
0	1		K242-u	LTDLLQEkHRTMSQE
0	1		S262-p	SKVETAEsRVsVLEs
0	1		S265-p	ETAEsRVsVLEsMID
0	1		S269-p	sRVsVLEsMIDDLQW
0	1		Y314-p	YGAGSSLyGGTITIN
0	1		K348-a	NRLCELEkLRQDFEE
0	1		K376-u	SAVEQVVkETPEYRC
1	4		S522-p	SGSALLQsQsstEDP
0	2		S524-p	SALLQsQsstEDPKD
0	3		S525-p	ALLQsQsstEDPKDE
0	1		T526-p	LLQsQsstEDPKDEP
0	3		S540-p	PAELKPDsEDLSsQS
1	2		S545-p	PDsEDLSsQSsAsKA
0	1		S547	sEDLSsQSsAsKAsQ
0	2		S548-p	EDLSsQSsAsKAsQE
0	1		S550-p	LSsQSsAsKAsQEDA
1	1		S553-p	QSsAsKAsQEDANEI
0	1		K610-m1	ERDSAKDkEkGkHDD
0	1		K612-u	DSAKDkEkGkHDDGR
0	1		K614-m1	AKDkEkGkHDDGRKK
0	2		Y650-p	MKLLLDMyRSAPKEQ
0	1		K769-m1	KLMSERIkSNQIHKL
0	1		K821-u	SNIGTGEkELGLRTQ
0	1		K857	QLELAQKKLHDFQDE
0	1		K874-a	ENSVTKEkDMFNFkR
0	1		K880-a	EkDMFNFkRAQEDIS

	mouse
K21-u	GTSMPPEkKTAVEDS
T34	DSGTTVETIKLGGVS
T43-p	KLGGVSStEELDIRT
T122	QGLGDLLTERKALVV
S136-p	VPEPEPDsDsNQERK
S138-p	EPEPDsDsNQERKDD
S172	SSSEEMESQLQERVE
K242	LTDLLQEKHHTMSQE
S262	GKVETAESRVSVLES
S265	ETAESRVSVLESMID
S269	SRVSVLESMIDDLQW
Y314	YGAGSSLYGGTITIN
K348	NRHCELEKLRQDFEE
K376	SAVEEVVKETPEYRC
S522-p	SGSALLQsQsstEDP
S524-p	SALLQsQsstEDPKD
S525-p	ALLQsQsstEDPKDE
T526-p	LLQsQsstEDPKDEP
S540-p	PTELKQDsEDLATHS
T545	QDsEDLATHSSALKA
S547	sEDLATHSSALKASQ
S548	EDLATHSSALKASQE
L550	LATHSSALKASQEDE
S553	HSSALKASQEDEVKS
K608	ERDSVKDKEkGKHDD
K610-u	DSVKDKEkGKHDDGR
K612	VKDKEkGKHDDGRKK
Y648	MKLLLDMYRSAPKEQ
K767	KLMSERIKSNQIHKL
K819	SNIGTGEKELGLRTQ
K855-u	QLELAQKkLHDFQDE
K872	ENSVTKEKDLFNFKR
K878	EKDLFNFKRAQEDIS

	rat
K21	GTSVPPEKKTAVEDS
T34	DSGTTVETIKLGGVS
T43	KLGGVSSTEELDIRT
T122	QGLGDLLTERKALVV
S136	VPEPEPDSDsNQERK
S138-p	EPEPDSDsNQERKDD
S172	SSSEEMESQLQERVE
K242	LTDLLQEKHHTMSQE
S262	GKVETAESRVSVLES
S265	ETAESRVSVLESMID
S269	SRVSVLESMIDDLQW
Y314	YGAGSSLYGGTITIN
K348	NRHCELEKLRQDFEE
K376	SAVEEVVKETPEYRC
S522	SGSALLQSQSSTEDA
S524	SALLQSQSSTEDAKD
S525	ALLQSQSSTEDAKDE
T526	LLQSQSSTEDAKDEP
S540-p	PTELKQDsEDLATHs
T545	QDsEDLATHssALKA
S547-p	sEDLATHssALKASQ
S548-p	EDLATHssALKASQE
L550	LATHssALKASQEDE
S553	HssALKASQEDEVKS
K608	ERDPVKDKDKGKHDD
K610	DPVKDKDKGKHDDGR
K612	VKDKDKGKHDDGRKK
Y648	MKLLLDMYRSAPKEQ
K767	KLMSERIKSNQIHKL
K819	SNIGTGEKELGLRTQ
K855	QLELAQKKLHDFQDE
K872	ENSVTKEKDLFNFKR
K878	EKDLFNFKRAQEDIS

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