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Protein Page:
PAK1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PAK1 a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, STE; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKA subfamily
Cellular Component: nuclear membrane; protein complex; focal adhesion; dendrite; intercellular junction; cytosol; Z disc; filamentous actin; ruffle; growth cone; axon; cytoplasm; plasma membrane
Molecular Function: collagen binding; protein serine/threonine kinase activity; protein binding; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity
Biological Process: positive regulation of JNK activity; axon guidance; exocytosis; wound healing; apoptosis; protein amino acid autophosphorylation; positive regulation of estrogen receptor signaling pathway; MAPKKK cascade; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; receptor clustering; T cell receptor signaling pathway; cellular response to insulin stimulus; branching morphogenesis of a tube; axonogenesis; T cell costimulation; positive regulation of stress fiber formation; actin cytoskeleton reorganization; response to hypoxia; dendrite development; innate immune response; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; neuromuscular junction development; neurite morphogenesis
Reference #:  Q13153 (UniProtKB)
Alt. Names/Synonyms: Alpha-PAK; MGC130000; MGC130001; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; PAK-1; PAK1; PAKalpha; Serine/threonine-protein kinase PAK 1; STE20 homolog, yeast
Gene Symbols: PAK1
Molecular weight: 60,647 Da
Basal Isoelectric point: 5.55  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  ErbB/HER Signaling  |  Growth And Differentiation Control by MAPKs  |  Microtubule Dynamics  |  TGF-ß Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PAK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: Y153‑p, Y201‑p, Y285‑p
cell adhesion, altered: S199‑p, S204‑p
cell cycle regulation: S174‑p
cell motility, altered: Y153‑p, S199‑p, Y201‑p, S204‑p, Y285‑p
cell motility, induced: Y153‑p, Y201‑p, Y285‑p
cytoskeletal reorganization: S21‑p, S144‑p
enzymatic activity, induced: S144‑p, Y153‑p, S174‑p, S199‑p, Y201‑p, S204‑p, Y285‑p, T423‑p
enzymatic activity, inhibited: T109‑p
intracellular localization: S21‑p, S144‑p, S199‑p, S204‑p
molecular association, regulation: S174‑p, T423‑p
protein conformation: S199‑p, S204‑p, T423‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
3 10 S21-p APPMRNTsTMIGAGS
0 1 K29-a TMIGAGSkDAGTLNH
1 0 T84 LPSDFEHTIHVGFDA
1 1 T109-p QWARLLQtSNITKsE
0 3 S115-p QtSNITKsEQKKNPQ
0 7 Y131-p VLDVLEFyNsKKtsN
0 2 S133-p DVLEFyNsKKtsNsQ
0 2 T136-p EFyNsKKtsNsQKyM
0 2 S137-p FyNsKKtsNsQKyMs
0 4 S139-p NsKKtsNsQKyMsFt
0 13 Y142-p KtsNsQKyMsFtDKs
7 140 S144-p sNsQKyMsFtDKsAE
0 15 T146-p sQKyMsFtDKsAEDy
1 25 S149-p yMsFtDKsAEDyNsS
4 23 Y153-p tDKsAEDyNsSNALN
0 4 S155-p KsAEDyNsSNALNVK
1 11 S174-p TPAVPPVsEDEDDDD
0 6 T185-p DDDDDDAtPPPVIAP
12 0 S199-p PRPEHTKsVyTRsVI
4 0 Y201-p PEHTKsVyTRsVIEP
10 6 S204-p TKsVyTRsVIEPLPV
9 30 T212-p VIEPLPVtPtRDVAt
0 4 T214-p EPLPVtPtRDVAtsP
0 2 T219-p tPtRDVAtsPIsPtE
0 9 S220-p PtRDVAtsPIsPtEN
1 18 S223-p DVAtsPIsPtENNtt
0 10 T225-p AtsPIsPtENNttPP
0 8 T229-p IsPtENNttPPDALt
0 31 T230-p sPtENNttPPDALtR
0 2 T236-p ttPPDALtRNTEKQK
0 1 S249-p QKKKPKMsDEEILEk
0 1 K256-a sDEEILEkLRsIVsV
0 1 S259-p EILEkLRsIVsVGDP
0 2 S262-p EkLRsIVsVGDPKKK
4 2 Y285-p QGASGTVyTAMDVAT
0 10 K420-u QITPEQSkRstMVGt
0 3 S422-p TPEQSkRstMVGtPY
22 10 T423-p PEQSkRstMVGtPYW
0 3 T427-p kRstMVGtPYWMAPE
0 9 Y474-p ENPLRALyLIATNGT
2606 : Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2601 : Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
  mouse

 
S21-p APPMRNTsTMIGAGS
K29 TMIGAGSKDTGTLNH
T84 LPSDFEHTIHVGFDA
T109-p QWARLLQtSNITKsE
S115-p QtSNITKsEQKKNPQ
Y131 VLDVLEFYNSKKTSN
S133 DVLEFYNSKKTSNSK
T136 EFYNSKKTSNSKKYM
S137 FYNSKKTSNSKKYMs
S139 NSKKTSNSKKYMsFT
Y142 KTSNSKKYMsFTDKS
S144-p SNSKKYMsFTDKSAE
T146 SKKYMsFTDKSAEDY
S149 YMsFTDKSAEDYNSS
Y153 TDKSAEDYNSSNTLN
S155 KSAEDYNSSNTLNVK
S174-p TPAVPPVsEDDEDDD
T185-p EDDDDDAtPPPVIAP
S199-p PRPEHTKsVYTRsVI
Y201 PEHTKsVYTRsVIEP
S204-p TKsVYTRsVIEPLPV
T212-p VIEPLPVtPtRDVAt
T214-p EPLPVtPtRDVAtsP
T219-p tPtRDVAtsPIsPtE
S220-p PtRDVAtsPIsPtEN
S223-p DVAtsPIsPtENNtt
T225-p AtsPIsPtENNttPP
T229-p IsPtENNttPPDALT
T230-p sPtENNttPPDALTR
T236 ttPPDALTRNTEKQK
S249-p QKKKPKMsDEEILEK
K256 sDEEILEKLRsIVsV
S259-p EILEKLRsIVsVGDP
S262-p EKLRsIVsVGDPKKK
Y285 QGASGTVYTAMDVAT
K420 QITPEQSKRstMVGt
S422-p TPEQSKRstMVGtPY
T423-p PEQSKRstMVGtPYW
T427-p KRstMVGtPYWMAPE
Y474 ENPLRALYLIATNGT
2606 : Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2601 : Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
  rat

 
S21-p APPMRNTsTMIGAGS
K29 TMIGAGSKDPGTLNH
T84-p LPSDFEHtIHVGFDA
T109 QWARLLQTSNITKSE
S115 QTSNITKSEQKKNPQ
Y131 VLDVLEFYNSKKTSN
S133 DVLEFYNSKKTSNSQ
T136 EFYNSKKTSNSQKYM
S137 FYNSKKTSNSQKYMs
S139 NSKKTSNSQKYMsFT
Y142 KTSNSQKYMsFTDKs
S144-p SNSQKYMsFTDKsAE
T146 SQKYMsFTDKsAEDY
S149-p YMsFTDKsAEDYNSS
Y153 TDKsAEDYNSSNTLN
S155 KsAEDYNSSNTLNVK
S174-p TPAVPPVsEDEDDDD
T184 EDDDDDATPPPVIAP
S198-p PRPEHTKsVYTRsVI
Y200 PEHTKsVYTRsVIEP
S203-p TKsVYTRsVIEPLPV
T211-p VIEPLPVtPTRDVAT
T213 EPLPVtPTRDVATSP
T218 tPTRDVATSPISPTE
S219 PTRDVATSPISPTEN
S222 DVATSPISPTENNTT
T224 ATSPISPTENNTTPP
T228 ISPTENNTTPPDALT
T229 SPTENNTTPPDALTR
T235 TTPPDALTRNTEKQK
S248 QKKKPKMSDEEILEK
K255 SDEEILEKLRSIVSV
S258 EILEKLRSIVSVGDP
S261 EKLRSIVSVGDPKKK
Y284 QGASGTVYTAMDVAT
K419 QITPEQSKRstMVGT
S421-p TPEQSKRstMVGTPY
T422-p PEQSKRstMVGTPYW
T426 KRstMVGTPYWMAPE
Y473 ENPLRALYLIATNGT
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