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Protein Page:
p70S6K (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
p70S6K an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.-; Translation; EC 2.7.11.1; AGC group; RSK family; p70 subfamily
Cellular Component: mitochondrial outer membrane; neuron projection; cell surface; mitochondrion; perinuclear region of cytoplasm; cytoplasm; synapse; cell junction; nucleus; cytosol
Molecular Function: protein binding; ribosomal protein S6 kinase activity; peptide binding; ATP binding; protein kinase activity
Biological Process: skeletal muscle contraction; TOR signaling pathway; response to glucagon stimulus; positive regulation of translation; apoptosis; positive regulation of smooth muscle cell proliferation; response to toxin; response to glucocorticoid stimulus; negative regulation of insulin receptor signaling pathway; response to lipopolysaccharide; positive regulation of smooth muscle cell migration; signal transduction; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; germ cell development; positive regulation of translational initiation; skeletal muscle atrophy; response to glucose stimulus; protein kinase B signaling cascade; response to wounding; response to nutrient; aging; response to drug; cell migration; phosphoinositide-mediated signaling; response to testosterone stimulus; response to electrical stimulus involved in regulation of muscle adaptation; response to ethanol; response to leucine; long-term memory; response to heat; response to mechanical stimulus; insulin receptor signaling pathway; regulation of glucose import; positive regulation of skeletal muscle growth; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Reference #:  P23443 (UniProtKB)
Alt. Names/Synonyms: 70 kDa ribosomal protein S6 kinase 1; KS6B1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; p70 S6K-alpha; p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; p70-S6K 1; P70S6K1; PS6K; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K-beta-1; S6K1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A
Gene Symbols: RPS6KB1
Molecular weight: 59,140 Da
Basal Isoelectric point: 6.21  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Angiogenesis  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Mitochondrial Control of Apoptosis  |  mTOR Signaling  |  PI3K/Akt Signaling  |  Protein Kinase C Signaling  |  TGF-├č Signaling  |  Translation: eIF4E and p70S6K
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

p70S6K

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell adhesion, altered: T412‑p, T444‑p, S447‑p
cell cycle regulation: T412‑p, T444‑p, S447‑p
translation, altered: T252‑p, T412‑p
activity, induced: S394‑p
enzymatic activity, induced: T252‑p, S394‑p, T412‑p, S427‑p, S434‑p, S441‑p, T444‑p, S447‑p
intracellular localization: T412‑p
molecular association, regulation: T444‑p, S447‑p
phosphorylation: T252‑p, T412‑p
protein conformation: T252‑p, S394‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
2 0 S40 DQPEDAGSEDELEEG
1 0 S53-p EGGQLNEsMDHGGVG
1 0 Y62 DHGGVGPYELGMEHC
0 1 T140 NAKDTAHTKAERNIL
0 2 R217 HQKGIIYRDLKPENI
0 2 S243-p DFGLCKEsIHDGtVt
0 2 T248-p KEsIHDGtVtHtFCG
0 5 T250-p sIHDGtVtHtFCGtI
26 10 T252-p HDGtVtHtFCGtIEY
0 6 T256-p VtHtFCGtIEYMAPE
0 1 K304-a NRKKTIDkILKCKLN
2 11 T390-p DSKFTRQtPVDsPDD
11 9 S394-p TRQtPVDsPDDSTLS
1 1 S403-p DDSTLSEsANQVFLG
298 3 T412-p NQVFLGFtyVAPSVL
0 2 Y413-p QVFLGFtyVAPSVLE
5 2 S427-p ESVKEKFsFEPKIRs
25 1 S434-p sFEPKIRsPRRFIGs
8 80 S441-p sPRRFIGsPRtPVsP
93 99 T444-p RFIGsPRtPVsPVKF
89 231 S447-p GsPRtPVsPVKFsPG
1 59 S452-p PVsPVKFsPGDFWGR
1 0 T470-p ASTANPQtPVEYPME
1 0 K516-a QAFPMISkRPEHLRM
9208 : Phospho-p70 S6 Kinase (Ser371) Antibody
9205 : Phospho-p70 S6 Kinase (Thr389) Antibody
9206 : Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb
9209 : Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody
9234 : Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
  p70S6K iso2  
S17 DQPEDAGSEDELEEG
S30 EGGQLNESMDHGGVG
Y39 DHGGVGPYELGMEHC
T117 NAKDTAHTKAERNIL
R194 HQKGIIYRDLKPENI
S220 DFGLCKESIHDGTVT
T225 KESIHDGTVTHtFCG
T227 SIHDGTVTHtFCGTI
T229-p HDGTVTHtFCGTIEY
T233 VTHtFCGTIEYMAPE
K281 NRKKTIDKILKCKLN
T367 DSKFTRQTPVDSPDD
S371 TRQTPVDSPDDSTLS
S380 DDSTLSESANQVFLG
T389-p NQVFLGFtYVAPSVL
Y390 QVFLGFtYVAPSVLE
S404 ESVKEKFSFEPKIRS
S411 SFEPKIRSPRRFIGS
S418 SPRRFIGSPRtPVsP
T421-p RFIGSPRtPVsPVKF
S424-p GSPRtPVsPVKFSPG
S429 PVsPVKFSPGDFWGR
T447 ASTANPQTPVEYPME
K493 QAFPMISKRPEHLRM
9209 : Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody
  mouse

 
S40 DQPEDAGSEDELEEG
S53 EGGQLNESMDHGGVG
Y62 DHGGVGPYELGMEHC
T140-p NAKDTAHtKAERNIL
R217-m1 HQKGIIYrDLKPENI
S243-p DFGLCKEsIHDGtVt
T248-p KEsIHDGtVtHtFCG
T250-p sIHDGtVtHtFCGtI
T252-p HDGtVtHtFCGtIEY
T256-p VtHtFCGtIEYMAPE
K304 NRKKTIDKILKCKLN
T390-p DSKFTRQtPVDsPDD
S394-p TRQtPVDsPDDSTLS
S403 DDSTLSESANQVFLG
T412-p NQVFLGFtYVAPSVL
Y413 QVFLGFtYVAPSVLE
S427-p ESVKEKFsFEPKIRs
S434-p sFEPKIRsPRRFIGs
S441-p sPRRFIGsPRtPVsP
T444-p RFIGsPRtPVsPVKF
S447-p GsPRtPVsPVKFsPG
S452-p PVsPVKFsPGDFWGR
T470 ASTANPQTPVEYPME
K516 QAFPMISKRPEHLRM
9208 : Phospho-p70 S6 Kinase (Ser371) Antibody
9205 : Phospho-p70 S6 Kinase (Thr389) Antibody
9206 : Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb
9234 : Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
  rat

► Hide Isoforms
 
S40-p DQPEDAGsEDELEEG
S53 EGGQLNESMDHGGVG
Y62-p DHGGVGPyELGMEHC
T140 NAKDTAHTKAERNIL
R217 HQKGIIYRDLKPENI
S243 DFGLCKESIHDGTVT
T248 KESIHDGTVTHtFCG
T250 SIHDGTVTHtFCGTI
T252-p HDGTVTHtFCGTIEY
T256 VTHtFCGTIEYMAPE
K304 NRKKTIDKILKCKLN
T390 DSKFTRQTPVDsPDD
S394-p TRQTPVDsPDDSTLS
S403 DDSTLSESANQVFLG
T412-p NQVFLGFtYVAPSVL
Y413 QVFLGFtYVAPSVLE
S427 ESVKEKFSFEPKIRs
S434-p SFEPKIRsPRRFIGs
S441-p sPRRFIGsPRtPVsP
T444-p RFIGsPRtPVsPVKF
S447-p GsPRtPVsPVKFsPG
S452-p PVsPVKFsPGDFWGR
T470 ASTANPQTPVEYPME
K516 QAFPMISKRPEHLRM
9208 : Phospho-p70 S6 Kinase (Ser371) Antibody
9205 : Phospho-p70 S6 Kinase (Thr389) Antibody
9206 : Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb
9234 : Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
  p70S6K iso2  
S17-p DQPEDAGsEDELEEG
S30 EGGQLNESMDHGGVG
Y39 DHGGVGPYELGMEHC
T117 NAKDTAHTKAERNIL
R194 HQKGIIYRDLKPENI
S220 DFGLCKESIHDGTVT
T225 KESIHDGTVTHTFCG
T227 SIHDGTVTHTFCGTI
T229 HDGTVTHTFCGTIEY
T233 VTHTFCGTIEYMAPE
K281 NRKKTIDKILKCKLN
T367 DSKFTRQTPVDSPDD
S371 TRQTPVDSPDDSTLS
S380 DDSTLSESANQVFLG
T389-p NQVFLGFtYVAPSVL
Y390 QVFLGFtYVAPSVLE
S404 ESVKEKFSFEPKIRS
S411 SFEPKIRSPRRFIGS
S418 SPRRFIGSPRtPVsP
T421-p RFIGSPRtPVsPVKF
S424-p GSPRtPVsPVKFSPG
S429 PVsPVKFSPGDFWGR
T447 ASTANPQTPVEYPME
K493 QAFPMISKRPEHLRM
  fruit fly

 
- gap
- gap
- gap
T126 NQKDTAHTRAERNIL
R203 HKLGIIYRDLKPENI
H229 DFGLCKEHIQEGIVT
I234 KEHIQEGIVTHTFCG
T236 HIQEGIVTHTFCGTI
T238 QEGIVTHTFCGTIEY
T242 VTHTFCGTIEYMAPE
T290 NRKKTIETILKAKLN
I376 DTRFTRQIPVDSPDD
S380 TRQIPVDSPDDTTLS
S389 DDTTLSESANLIFQG
T398 NLIFQGFTYVAPSIL
Y399 LIFQGFTYVAPSILE
- gap
S418 ANRMPARSPRRTPRQ
- gap
- gap
- gap
- gap
- gap
- gap
9209 : Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody
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