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Protein Page:
MKK3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MKK3 a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. A deletion and two point mutants found in colon cancer cell lines. Three splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Kinase, protein; Protein kinase, STE; STE group; STE7 family
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: MAP kinase kinase activity; protein binding; protein-tyrosine kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of protein kinase activity; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; regulation of cytokine biosynthetic process; cardiac muscle contraction
Reference #:  P46734 (UniProtKB)
Alt. Names/Synonyms: Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; mitogen-activated protein kinase kinase 3; MKK3; MP2K3; PRKMK3
Gene Symbols: MAP2K3
Molecular weight: 39,318 Da
Basal Isoelectric point: 7.05  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  IL6 Signaling  |  Regulation of P38 MAPKs  |  TGF-├č Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MKK3

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: T222‑p
transcription, induced: S218‑p, T222‑p
activity, induced: T222‑p
enzymatic activity, induced: S218‑p, T222‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S3-p _____MEsPASSQPA
0 1 S15-p QPASMPQsKGKSKRK
0 1 S28 RKKDLRISCMSKPPA
0 1 S31 DLRISCMSKPPAPNP
0 7 T39-p KPPAPNPtPPRNLDS
0 6 K80-ub GAYGVVEkVRHAQSG
0 1 K192-ub SVIHRDVkPSNVLIN
0 1 K200 PSNVLINKEGHVKMC
0 2 S212-p KMCDFGIsGyLVDsV
0 2 Y214-p CDFGIsGyLVDsVAK
14 55 S218-p IsGyLVDsVAKtMDA
4 3 T222-p LVDsVAKtMDAGCKP
0 2 Y230-p MDAGCKPyMAPERIN
0 3 K243-ub INPELNQkGyNVKsD
0 1 Y245-p PELNQkGyNVKsDVW
0 2 S249-p QkGyNVKsDVWsLGI
0 1 S253-p NVKsDVWsLGITMIE
0 1 K279-ub GTPFQQLkQVVEEPS
0 1 K340-ub TDIAAFVkEILGEDS
9231 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody
9236 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb
12280 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb
  mouse

 
S3 _____MESPAASPPA
T15 PPASLPQTKGKSKRK
S28-p RKKDLRIsCVsKPPV
S31-p DLRIsCVsKPPVSNP
T39 KPPVSNPTPPRNLDS
K80-ub GAYGVVEkVRHAQSG
K192 SVIHRDVKPSNVLIN
K200-ac PSNVLINkEGHVKMC
S212 KMCDFGISGYLVDsV
Y214 CDFGISGYLVDsVAK
S218-p ISGYLVDsVAKTMDA
T222 LVDsVAKTMDAGCKP
Y230 MDAGCKPYMAPERIN
K243-ub INPELNQkGYNVKSD
Y245 PELNQkGYNVKSDVW
S249 QkGYNVKSDVWSLGI
S253 NVKSDVWSLGITMIE
K279 GTPFQQLKQVVEEPS
K340 TDIAAFVKEILGEDS
9231 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody
9236 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb
12280 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb
  rat

 
S75 ATVRSMESPAASPPA
T87 PPASLPQTKGKSKRK
S100 RKRDLRISCVSKPPV
S103 DLRISCVSKPPVSNP
T111 KPPVSNPTPPRNLDS
K152 GAYGVVEKVRHAQSG
K264 SVIHRDVKPSNVLIN
K272 PSNVLINKEGHVKMC
S284 KMCDFGISGYLVDsV
Y286 CDFGISGYLVDsVAK
S290-p ISGYLVDsVAKTMDA
T294 LVDsVAKTMDAGCKP
Y302 MDAGCKPYMAPERIN
K315 INPELNQKGYNVKSD
Y317 PELNQKGYNVKSDVW
S321 QKGYNVKSDVWSLGI
S325 NVKSDVWSLGITMIE
K351 GTPFQQLKQVVEEPS
K412 TDIAAFVKEILGEDS
9231 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody
9236 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb
12280 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb
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