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Protein Page:
MEK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MEK1 a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.12.2; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); Kinase, protein; STE group; STE7 family
Cellular Component: dendrite cytoplasm; Golgi apparatus; focal adhesion; mitochondrion; early endosome; perikaryon; cell cortex; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; plasma membrane; microtubule organizing center; nucleus
Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity
Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; activation of MAPK activity; protein heterooligomerization; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; cell motility involved in cell locomotion; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; chemotaxis; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; negative regulation of cell proliferation; positive regulation of Ras GTPase activity; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; keratinocyte differentiation; regulation of stress-activated MAPK cascade; cell proliferation; Ras protein signal transduction; regulation of vascular smooth muscle contraction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; response to oxidative stress; positive regulation of Ras protein signal transduction; cell motility; positive regulation of cell differentiation; positive regulation of cell migration
Reference #:  Q02750 (UniProtKB)
Alt. Names/Synonyms: Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; mitogen-activated protein kinase kinase 1; MKK1; MP2K1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Symbols: MAP2K1
Molecular weight: 43,439 Da
Basal Isoelectric point: 6.18  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Angiogenesis  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  IL6 Signaling  |  Insulin Receptor Signaling  |  T Cell Receptor Signaling  |  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MEK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell cycle regulation: S218‑p, S222‑p
cell growth, altered: S218‑p, S222‑p, S298‑p
enzymatic activity, induced: S218‑p, S222‑p
enzymatic activity, inhibited: S212‑p, T286‑p, T292‑p
intracellular localization: T386‑p, T388‑p
molecular association, regulation: T292‑p, S298‑p, T386‑p
phosphorylation: T286‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 D16-ca IQLNPAPdGSAVNGT
1 0 S24 GSAVNGTSSAETNLE
0 1 S25 SAVNGTSSAETNLEA
0 1 K36-ub NLEALQKkLEELELD
0 1 K57-ub LEAFLTQkQKVGELk
0 1 K64-ub kQKVGELkDDDFEKI
1 0 S72 DDDFEKISELGAGNG
0 9 K97-ub SGLVMARkLIHLEIk
0 13 K104-ub kLIHLEIkPAIRNQI
0 7 K168-ub IPEQILGkVSIAVIk
0 6 K175-ub kVSIAVIkGLTYLRE
0 27 K192-ub KIMHRDVkPSNILVN
0 12 K205-ub VNSRGEIkLCDFGVs
3 4 S212-p kLCDFGVsGQLIDsM
92 77 S218-p VsGQLIDsMANsFVG
87 205 S222-p LIDsMANsFVGTRSY
0 3 S231-p VGTRSYMsPERLQGT
0 1 D282-ca FGCQVEGdAAEtPPR
6 19 T286-p VEGdAAEtPPRPRtP
10 2 T292-p EtPPRPRtPGRPLsS
17 0 S298-p RtPGRPLsSyGMDSR
1 1 Y300-p PGRPLsSyGMDSRPP
0 2 K353-ub PAERADLkQLMVHAF
0 8 S385-p TIGLNQPstPtHAAG
4 53 T386-p IGLNQPstPtHAAGV
1 10 T388-p LNQPstPtHAAGV__
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
2338 : Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
9127 : Phospho-MEK1 (Thr286) Antibody
9128 : Phospho-MEK1 (Ser298) Antibody
  mouse

 
D16 IQLNPAPDGSAVNGT
S24 GSAVNGTSsAETNLE
S25-p SAVNGTSsAETNLEA
K36 NLEALQKKLEELELD
K57 LEAFLTQKQKVGELK
K64 KQKVGELKDDDFEKI
S72 DDDFEKISELGAGNG
K97 SGLVMARKLIHLEIk
K104-ub KLIHLEIkPAIRNQI
K168-ub IPEQILGkVSIAVIk
K175-ub kVSIAVIkGLTYLRE
K192-ub KIMHRDVkPSNILVN
K205 VNSRGEIKLCDFGVS
S212 KLCDFGVSGQLIDsM
S218-p VSGQLIDsMANsFVG
S222-p LIDsMANsFVGTRSY
S231-p VGTRSYMsPERLQGT
D282 FGCHVEGDAAEtPPR
T286-p VEGDAAEtPPRPRtP
T292-p EtPPRPRtPGRPLsS
S298-p RtPGRPLsSYGMDSR
Y300 PGRPLsSYGMDSRPP
K353-ub PAERADLkQLMVHAF
S385-p TIGLNQPstPtHAAS
T386-p IGLNQPstPtHAASI
T388-p LNQPstPtHAASI__
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
2338 : Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
9127 : Phospho-MEK1 (Thr286) Antibody
9128 : Phospho-MEK1 (Ser298) Antibody
  rat

 
D16 IQLNPAPDGSAVNGT
S24 GSAVNGTSSAETNLE
S25 SAVNGTSSAETNLEA
K36 NLEALQKKLEELELD
K57 LEAFLTQKQKVGELK
K64 KQKVGELKDDDFEKI
S72 DDDFEKISELGAGNG
K97 SGLVMARKLIHLEIK
K104 KLIHLEIKPAIRNQI
K168 IPEQILGKVSIAVIK
K175 KVSIAVIKGLTYLRE
K192-ub KIMHRDVkPSNILVN
K205 VNSRGEIKLCDFGVs
S212-p KLCDFGVsGQLIDsM
S218-p VsGQLIDsMANsFVG
S222-p LIDsMANsFVGTRSY
S231 VGTRSYMSPERLQGT
D282 FGCQVEGDAAEtPPR
T286-p VEGDAAEtPPRPRtP
T292-p EtPPRPRtPGRPLsS
S298-p RtPGRPLsSYGMDSR
Y300 PGRPLsSYGMDSRPP
K353 PAERADLKQLMVHAF
S385 TIGLNQPStPTHAAS
T386-p IGLNQPStPTHAASI
T388 LNQPStPTHAASI__
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
2338 : Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
9127 : Phospho-MEK1 (Thr286) Antibody
9128 : Phospho-MEK1 (Ser298) Antibody
  rabbit

 
D16 IQLNPAPDGSAVNGT
S24-p GSAVNGTsSAETNLE
S25 SAVNGTsSAETNLEA
K36 NLEALQKKLEELELD
K57 LEAFLTQKQKVGELK
K64 KQKVGELKDDDFEKI
S72-p DDDFEKIsELGAGNG
K97 SGLVMARKLIHLEIK
K104 KLIHLEIKPAIRNQI
K168 IPEQILGKVSIAVIK
K175 KVSIAVIKGLTYLRE
K192 KIMHRDVKPSNILVN
K205 VNSRGEIKLCDFGVS
S212 KLCDFGVSGQLIDsM
S218-p VSGQLIDsMANsFVG
S222-p LIDsMANsFVGTRSY
S231 VGTRSYMSPERLQGT
D282 FGCQVEGDAAETPPR
T286 VEGDAAETPPRPRtP
T292-p ETPPRPRtPGRPLsS
S298-p RtPGRPLsSyGMDSR
Y300-p PGRPLsSyGMDSRPP
K353 PAERADLKQLMVHAF
S385 TIGLNQPSTPTHAAG
T386 IGLNQPSTPTHAAGV
T388 LNQPSTPTHAAGV__
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
2338 : Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
3958 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)
9121 : Phospho-MEK1/2 (Ser217/221) Antibody
9154 : Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
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