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Protein Page:
LKB1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LKB1 a S/T protein kinase of the CAMKL family. A tumor suppressor that helps control cell structure, polarity, apoptosis and energy homeostasis. Phosphorylates AGS3 (activator of G-protein signaling 3) GPR domains, regulating the interaction of GPR-containing proteins with G-proteins. A cytosolic protein complex comprised of LKB1, the pseudokinase STRAD, and the MO25 scaffold protein. Activates AMPK and several related protein kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. The Lkb1/Strad/Mo25 complex can polarize single epithelial cells, leading to the formation of a brush border containing microvilli on the apical surface. Mutation in the corresponding gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Autophagy; Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; LKB subfamily
Cellular Component: membrane; mitochondrion; cytoplasm; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; magnesium ion binding; protein complex binding; protein kinase activator activity; LRR domain binding; ATP binding
Biological Process: Golgi localization; response to glucagon stimulus; protein heterooligomerization; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; glucose homeostasis; response to lipid; T cell receptor signaling pathway; protein amino acid phosphorylation; anoikis; negative regulation of cell proliferation; positive regulation of gluconeogenesis; regulation of fatty acid biosynthetic process; vasculature development; tissue homeostasis; regulation of cell growth; cell cycle arrest; positive thymic T cell selection; regulation of Wnt receptor signaling pathway; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; axonogenesis; establishment of cell polarity; insulin receptor signaling pathway; energy reserve metabolic process; activation of protein kinase activity; autophagy; positive regulation of axonogenesis; negative regulation of cell growth; response to ionizing radiation; response to DNA damage stimulus; spermatid development
Reference #:  Q15831 (UniProtKB)
Alt. Names/Synonyms: LKB1; PAR-4; PAR4; PJS; polarization-related protein LKB1; Renal carcinoma antigen NY-REN-19; serine/threonine kinase 11; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; STK11
Gene Symbols: STK11
Molecular weight: 48,636 Da
Basal Isoelectric point: 7.12  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

LKB1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S307‑p
apoptosis, inhibited: T189‑p
cell growth, altered: S307‑p, S428‑p
enzymatic activity, induced: T185‑p, T336‑p, T363‑p, T402‑p, S428‑p
intracellular localization: S307‑p, S428‑p
molecular association, regulation: S307‑p, S428‑p
phosphorylation: S428‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 292 S31-p TFIHRIDstEVIyQP
0 35 T32-p FIHRIDstEVIyQPR
1 11 Y36-p IDstEVIyQPRRKRA
1 0 K44-ac QPRRKRAkLIGkYLM
1 0 K48-ac KRAkLIGkYLMGDLL
1 0 Y60 DLLGEGSYGKVKEVL
2 0 K96-ac PNGEANVkkEIQLLR
2 0 K97-ac NGEANVkkEIQLLRR
1 0 Y156 PVCQAHGYFCQLIDG
1 0 Y166 QLIDGLEYLHSQGIV
1 0 T185-p KPGNLLLtTGGtLKI
2 0 T189-p LLLtTGGtLKISDLG
0 1 S240-p GFKVDIWsAGVTLYN
3 0 Y261 PFEGDNIYKLFENIG
1 0 K296-ac MLEYEPAkRFSIRQI
2 0 S307-p IRQIRQHsWFRkKHP
1 0 K311-ac RQHsWFRkKHPPAEA
1 0 S325 APVPIPPSPDTKDRW
1 0 S334-p DTKDRWRsMtVVPYL
2 0 T336-p KDRWRsMtVVPYLED
3 0 Y362 DIEDDIIYtQDFTVP
4 0 T363-p IEDDIIYtQDFTVPG
1 0 - gap
0 1 S401-p GTEAAQLstKSRAEG
1 0 T402-p TEAAQLstKSRAEGR
1 0 K416-ac RAPNPARkACSASSk
0 1 S421 ARkACSASSkIRRLs
1 0 K423-ac kACSASSkIRRLsAC
21 5 S428-p SSkIRRLsACkQQ__
1 0 K431-ac IRRLsACkQQ_____
3054 : Phospho-LKB1 (Thr189) Antibody
3055 : Phospho-LKB1 (Ser334) Antibody
3482 : Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb
  LKB1 iso2  
S31 TFIHRIDSTEVIYQP
T32 FIHRIDSTEVIYQPR
Y36 IDSTEVIYQPRRKRA
K44 QPRRKRAKLIGKYLM
K48 KRAKLIGKYLMGDLL
Y60 DLLGEGSYGKVKEVL
K96 PNGEANVKKEIQLLR
K97 NGEANVKKEIQLLRR
Y156 PVCQAHGYFCQLIDG
Y166 QLIDGLEYLHSQGIV
T185 KPGNLLLTTGGTLKI
T189 LLLTTGGTLKISDLG
S240 GFKVDIWSAGVTLYN
Y261 PFEGDNIYKLFENIG
K296 MLEYEPAKRFSIRQI
S307 IRQIRQHSWFRKKHP
K311 RQHSWFRKKHPPAEA
S325 APVPIPPSPDTKDRW
S334 DTKDRWRSMTVVPYL
T336 KDRWRSMTVVPYLED
Y362 DIEDDIIYTQDFTVP
T363 IEDDIIYTQDFTVPG
S399-p DLSGEEAsRPAPQ__
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
S31-p TFIHRIDstEVIyQP
T32-p FIHRIDstEVIyQPR
Y36-p IDstEVIyQPRRKRA
K44 QPRRKRAKLIGKYLM
K48 KRAKLIGKYLMGDLL
Y60-p DLLGEGSyGKVKEVL
K96 PNGEANVKKEIQLLR
K97 NGEANVKKEIQLLRR
Y156-p PVCQAHGyFRQLIDG
Y166-p QLIDGLEyLHSQGIV
T185 KPGNLLLTTNGTLKI
T189 LLLTTNGTLKISDLG
S240 GFKVDIWSAGVTLYN
Y261-p PFEGDNIyKLFENIG
K296 MLEYEPAKRFSIRQI
S307 IRQIRQHSWFRKKHP
K311 RQHSWFRKKHPLAEA
S325-p ALVPIPPsPDTKDRW
S334 DTKDRWRSMtVVPYL
T336-p KDRWRSMtVVPYLED
Y365-p DIEDGIIytQDFTVP
T366-p IEDGIIytQDFTVPG
- gap
S403 NGTEPQLSSKVKPEG
S404 GTEPQLSSKVKPEGR
K420 GTANPARKVCSsNKI
S424-p PARKVCSsNKIRRLs
K426 RKVCSsNKIRRLsAC
S431-p sNKIRRLsACKQQ__
K434 IRRLsACKQQ_____
3482 : Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb
  rat

 
S31-p TFIHRIDsTEVIYQP
T32 FIHRIDsTEVIYQPR
Y36 IDsTEVIYQPRRKRA
K44 QPRRKRAKLIGKYLM
K48 KRAKLIGKYLMGDLL
Y60 DLLGEGSYGKVKEVL
K96 PNGEANVKKEIQLLR
K97 NGEANVKKEIQLLRR
Y156 PVCQAHGYFRQLIDG
Y166 QLIDGLEYLHSQGIV
T185 KPGNLLLTTNGTLKI
T189 LLLTTNGTLKISDLG
S240 GFKVDIWSAGVTLYN
Y261 PFEGDNIYKLFENIG
K296 MLEYEPAKRFSIRQI
S307 IRQIRQHSWFRKKHP
K311 RQHSWFRKKHPLAEA
S325 ALVPIPPSPDTKDRW
S334 DTKDRWRSMTVVPYL
T336 KDRWRSMTVVPYLED
Y365 DIEDGIIYTQDFTVP
T366 IEDGIIYTQDFTVPG
- gap
S403 NGTEPQLSSKVKPEG
S404 GTEPQLSSKVKPEGR
K420 GAANPARKVCSSNKI
S424 PARKVCSSNKIRRLs
K426 RKVCSSNKIRRLsAC
S431-p SNKIRRLsACKQQ__
K434 IRRLsACKQQ_____
  pig

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y62 PVCQAHGYFCQLVDG
Y72 QLVDGLEYLHSQGIV
T91 KPGNLLLTTGGTLKI
T95 LLLTTGGTLKISDLG
S146 GFKVDIWSAGVTLYN
Y167 PFEGDNIYKLFENIG
K202 MLEYEPAKRFSIQQI
S213 IQQIRQHSWFRKKHP
K217 RQHSWFRKKHPPAEE
S231 EPVPIPPSTDCKDRW
G240 DCKDRWRGMTVVPYL
T242 KDRWRGMTVVPYLED
Y270 DIEDDIIYTQDFTVP
T271 IEDDIIYTQDFTVPG
- gap
S309 GTESVQLSAKSRAER
A310 TESVQLSAKSRAERR
K327 ATSNPTRKACSASSK
S332 TRKACSASSKIRRLs
K334 KACSASSKIRRLsAC
S339-p SSKIRRLsACKQQ__
K342 IRRLsACKQQ_____
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