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Protein Page:
SPT5 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SPT5 a transcription elongation factor. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Two alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: protein binding; enzyme binding; protein heterodimerization activity; chromatin binding
Biological Process: transcription from RNA polymerase II promoter; response to organic substance; chromatin remodeling; mRNA capping; viral reproduction; RNA elongation; positive regulation of viral transcription; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of RNA elongation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell cycle; positive regulation of macroautophagy
Reference #:  O00267 (UniProtKB)
Alt. Names/Synonyms: DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; FLJ34157; hSPT5; SPT5; SPT5H; suppressor of Ty 5 homolog (S. cerevisiae); SUPT5H; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; Transcription elongation factor SPT5
Gene Symbols: SUPT5H
Molecular weight: 121,000 Da
Basal Isoelectric point: 4.95  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SPT5

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: T768‑p, T775‑p, T784‑p, T791‑p, T799‑p, T806‑p, T814‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S2 ______MSDSEDSNF
0 1 S4 ____MSDSEDSNFSE
0 1 S7 _MSDSEDSNFSEEED
0 12 S32-p EVDEERRsAAGsEKE
0 11 S36-p ERRsAAGsEKEEEPE
0 1 Y86-p EADVDDEyEDEDQWE
0 2 S108-p EKEEIEAsNIDNVVL
0 1 S120-p VVLDEDRsGARRLQN
0 96 Y140-p REEELGEyyMkKYAK
0 5 Y141-p EEELGEyyMkKYAKS
0 1 K143-ub ELGEyyMkKYAKSSV
0 1 Y155-p SSVGETVyGGsDELS
0 1 S158-p GETVyGGsDELSDDI
0 1 K199-ub TAISLMRkFIAYQFT
0 1 T208-p IAYQFTDtPLQIkSV
0 2 K213-ub TDtPLQIkSVVAPEH
0 1 Y230-p GYIYVEAyKQTHVKQ
0 1 K258-ub NQQMVPIkEMTDVLK
0 3 Y295-p DDIAQVDyVEPSQNT
0 2 K317-ac RIDYDRIkARMSLkD
0 1 K323-ac IkARMSLkDWFAkRK
0 1 K323-ub IkARMSLkDWFAkRK
0 2 K328-ac SLkDWFAkRKKFKRP
0 1 S375-p RKGFLFKsFAMSAVI
0 6 K650-ub LVLAGGSkPRDVTNF
0 7 A663 NFTVGGFAPMsPRIS
3 59 S666-p VGGFAPMsPRISsPM
0 9 S671-p PMsPRISsPMHPSAG
0 6 R681-m1 HPSAGGQrGGFGsPG
1 1 R681-m2 HPSAGGQrGGFGsPG
0 1 S686-p GQrGGFGsPGGGSGG
0 18 R696-m1 GGSGGMSrGrGRRDN
1 0 R696-m2 GGSGGMSrGrGRRDN
1 0 R698-m2 SGGMSrGrGRRDNEL
0 1 T709-p DNELIGQtVRISQGP
0 1 Y717-p VRISQGPykGyIGVV
0 9 K718-ub RISQGPykGyIGVVK
0 1 Y720-p SQGPykGyIGVVKDA
0 1 K725 kGyIGVVKDATESTA
0 1 S744-p HSTCQTIsVDRQRLT
0 1 T751 sVDRQRLTTVGSRRP
0 6 T762-p SRRPGGMtStyGRtP
0 1 T764-p RPGGMtStyGRtPMy
0 29 Y765-p PGGMtStyGRtPMyG
1 13 T768-p MtStyGRtPMyGsQt
0 33 Y771-p tyGRtPMyGsQtPMy
1 7 S773-p GRtPMyGsQtPMyGs
3 39 T775-p tPMyGsQtPMyGsGs
0 37 Y778-p yGsQtPMyGsGsRtP
0 4 S780-p sQtPMyGsGsRtPMy
1 3 S782-p tPMyGsGsRtPMyGs
2 11 T784-p MyGsGsRtPMyGsQt
0 44 Y787-p sGsRtPMyGsQtPLQ
0 18 S789-p sRtPMyGsQtPLQDG
2 81 T791-p tPMyGsQtPLQDGsR
0 13 S797-p QtPLQDGsRtPHyGs
1 40 T799-p PLQDGsRtPHyGsQt
0 28 Y802-p DGsRtPHyGsQtPLH
0 26 S804-p sRtPHyGsQtPLHDG
1 55 T806-p tPHyGsQtPLHDGsR
0 5 S812-p QtPLHDGsRtPAQSG
1 4 T814-p PLHDGsRtPAQSGAW
0 1 S917-gl SYHQVAPsPAGYQNT
0 49 T1034-p SEHLEPItPtkNNKV
0 27 T1036-p HLEPItPtkNNKVKV
0 2 K1037-ub LEPItPtkNNKVKVI
  SPT5 iso2  
S2 ______MSDSEDSNF
S4 ____MSDSEDSNFSE
S7 _MSDSEDSNFSEEED
S32 EVDEERRSAAGSEKE
S36 ERRSAAGSEKEEEPE
Y86 EADVDDEYEDEDQWE
S104-p EDILEKAsNIDNVVL
S116-p VVLDEDRsGARRLQN
Y136 REEELGEYYMKKYAK
Y137 EEELGEYYMKKYAKS
K139 ELGEYYMKKYAKSSV
Y151 SSVGETVYGGSDELS
S154 GETVYGGSDELSDDI
K195 TAISLMRKFIAYQFT
T204 IAYQFTDTPLQIKSV
K209 TDTPLQIKSVVAPEH
Y226 GYIYVEAYKQTHVKQ
K254 NQQMVPIKEMTDVLK
Y291 DDIAQVDYVEPSQNT
K313 RIDYDRIKARMSLKD
K319 IKARMSLKDWFAKRK
K319 IKARMSLKDWFAKRK
K324 SLKDWFAKRKKFKRP
S371 RKGFLFKSFAMSAVI
K646 LVLAGGSKPRDVTNF
A659 NFTVGGFAPMSPRIS
S662 VGGFAPMSPRISSPM
S667 PMSPRISSPMHPSAG
R677 HPSAGGQRGGFGSPG
R677 HPSAGGQRGGFGSPG
S682 GQRGGFGSPGGGSGG
R692 GGSGGMSRGRGRRDN
R692 GGSGGMSRGRGRRDN
R694 SGGMSRGRGRRDNEL
T705 DNELIGQTVRISQGP
Y713 VRISQGPYKGYIGVV
K714 RISQGPYKGYIGVVK
Y716 SQGPYKGYIGVVKDA
K721 KGYIGVVKDATESTA
S740 HSTCQTISVDRQRLT
T747 SVDRQRLTTVGSRRP
T758 SRRPGGMTSTYGRTP
T760 RPGGMTSTYGRTPMY
Y761 PGGMTSTYGRTPMYG
T764 MTSTYGRTPMYGSQT
Y767 TYGRTPMYGSQTPMY
S769 GRTPMYGSQTPMYGS
T771 TPMYGSQTPMYGSGS
Y774 YGSQTPMYGSGSRTP
S776 SQTPMYGSGSRTPMY
S778 TPMYGSGSRTPMYGS
T780 MYGSGSRTPMYGSQT
Y783 SGSRTPMYGSQTPLQ
S785 SRTPMYGSQTPLQDG
T787 TPMYGSQTPLQDGSR
S793 QTPLQDGSRTPHYGS
T795 PLQDGSRTPHYGSQT
Y798 DGSRTPHYGSQTPLH
S800 SRTPHYGSQTPLHDG
T802 TPHYGSQTPLHDGSR
S808 QTPLHDGSRTPAQSG
T810 PLHDGSRTPAQSGAW
S913 SYHQVAPSPAGYQNT
T1030 SEHLEPITPTKNNKV
T1032 HLEPITPTKNNKVKV
K1033 LEPITPTKNNKVKVI
  mouse

 
S2-p ______MsDsEDsNF
S4-p ____MsDsEDsNFSE
S7-p _MsDsEDsNFSEEED
S32-p EVEEDQRsAAGsEKE
S36-p DQRsAAGsEKEEEPE
Y84 EADVDDEYEDEDQWE
S106 EKEEIEASNIDNVVL
S118 VVLDEDRSGARRLQN
Y138 REEELGEYYMKKYAK
Y139 EEELGEYYMKKYAKS
K141 ELGEYYMKKYAKSSV
Y153 SSVGETVYGGSDELS
S156 GETVYGGSDELSDDI
K197 TAISLMRKFIAYQFT
T206 IAYQFTDTPLQIkSV
K211-ub TDTPLQIkSVVAPEH
Y228 GYIYVEAYKQTHVKQ
K256 NQQMVPIKEMTDVLK
Y293-p DDIAQVDyVEPSQNT
K315 RIDYDRIKARMSLKD
K321 IKARMSLKDWFAKRK
K321 IKARMSLKDWFAKRK
K326 SLKDWFAKRKKFKRP
S373 RKGFLFKSFAMSAVI
K648-ub LVLAGGSkPRDVTNL
T661-p NLTVGGFtPMsPRIS
S664-p VGGFtPMsPRISsPM
S669-p PMsPRISsPMHPSAE
H679 HPSAEGQHGGFGSPG
H679 HPSAEGQHGGFGSPG
S684 GQHGGFGSPGGMSrG
R690-m1 GSPGGMSrGRGRRDN
R690 GSPGGMSRGRGRRDN
R692 PGGMSrGRGRRDNEL
T703 DNELIGQTVRISQGP
Y711 VRISQGPYkGYIGVV
K712-ub RISQGPYkGYIGVVk
Y714 SQGPYkGYIGVVkDA
K719-ub kGYIGVVkDATESTA
S738 HSTCQTISVDRQRLt
T745-p SVDRQRLtTVDSQRP
T756 SQRPGGMTSTYGRTP
T758 RPGGMTSTYGRTPMY
Y759 PGGMTSTYGRTPMYG
T762 MTSTYGRTPMYGsQt
Y765 TYGRTPMYGsQtPMy
S767-p GRTPMYGsQtPMyGs
T769-p TPMYGsQtPMyGsGs
Y772-p YGsQtPMyGsGsRTP
S774-p sQtPMyGsGsRTPMY
S776-p tPMyGsGsRTPMYGS
T778 MyGsGsRTPMYGSQT
Y781 sGsRTPMYGSQTPLQ
S783 sRTPMYGSQTPLQDG
T785 TPMYGSQTPLQDGSR
S791 QTPLQDGSRtPHyGs
T793-p PLQDGSRtPHyGsQt
Y796-p DGSRtPHyGsQtPLH
S798-p SRtPHyGsQtPLHDG
T800-p tPHyGsQtPLHDGSR
S806 QtPLHDGSRTPAQSG
T808 PLHDGSRTPAQSGAW
S911 SYHQVAPSPAGYQNT
T1028-p SEHLEPItPtkNNKV
T1030-p HLEPItPtkNNKVKV
K1031-ub LEPItPtkNNKVKVI
  rat

 
S2 ______MSDSEDSNF
S4 ____MSDSEDSNFSE
S7 _MSDSEDSNFSEEED
S32-p EVEEDRRsAAGsEKE
S36-p DRRsAAGsEKEEEPE
Y85 EADVDDEYEDEDQWE
S107 EKEEIEASNIDNVVL
S119 VVLDEDRSGARRLQN
Y139 REEELGEYYMKKYAK
Y140 EEELGEYYMKKYAKS
K142 ELGEYYMKKYAKSSV
Y154 SSVGETVYGGSDELS
S157 GETVYGGSDELSDDI
K198 TAISLMRKFIAYQFT
T207 IAYQFTDTPLQIKSV
K212 TDTPLQIKSVVAPEH
Y229 GYIYVEAYKQTHVKQ
K257 NQQMVPIKEMTDVLK
Y294 DDIAQVDYVEPSQNT
K316 RIDYDRIKARMSLKD
K322 IKARMSLKDWFAkRK
K322 IKARMSLKDWFAkRK
K327-ac SLKDWFAkRKKFKRP
S374 RKGFLFKSFAMSAVI
K649 LVLAGGSKPRDVTNL
T662-p NLTVGGFtPMsPRIS
S665-p VGGFtPMsPRISSPM
S670 PMsPRISSPMHPSAE
H680 HPSAEGQHGGFGSPG
H680 HPSAEGQHGGFGSPG
S685 GQHGGFGSPGGMSrG
R691-m1 GSPGGMSrGRGRRDN
R691 GSPGGMSRGRGRRDN
R693 PGGMSrGRGRRDNEL
T704 DNELIGQTVRISQGP
Y712 VRISQGPYKGYIGVV
K713 RISQGPYKGYIGVVK
Y715 SQGPYKGYIGVVKDA
K720 KGYIGVVKDATESTA
S739 HSTCQTISVDRQRLT
T746 SVDRQRLTTVDSQRP
T757 SQRPGGMTSTYGRTP
T759 RPGGMTSTYGRTPMy
Y760 PGGMTSTYGRTPMyG
T763 MTSTYGRTPMyGSQT
Y766-p TYGRTPMyGSQTPMy
S768 GRTPMyGSQTPMyGS
T770 TPMyGSQTPMyGSGS
Y773-p yGSQTPMyGSGSRTP
S775 SQTPMyGSGSRTPMY
S777 TPMyGSGSRTPMYGS
T779 MyGSGSRTPMYGSQT
Y782 SGSRTPMYGSQTPLQ
S784 SRTPMYGSQTPLQDG
T786 TPMYGSQTPLQDGSR
S792 QTPLQDGSRTPHYGS
T794 PLQDGSRTPHYGSQT
Y797 DGSRTPHYGSQTPLH
S799 SRTPHYGSQTPLHDG
T801 TPHYGSQTPLHDGSR
S807 QTPLHDGSRTPAQSG
T809 PLHDGSRTPAQSGAW
S912 SYHQVAPSPAGYQNT
T1029-p SEHLEPItPTKNNKV
T1031 HLEPItPTKNNKVKV
K1032 LEPItPTKNNKVKVI
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