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Protein Page:
SPT5 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
SPT5 a transcription elongation factor. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Two alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription, coactivator/corepressor
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: protein binding; enzyme binding; protein heterodimerization activity; chromatin binding
Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of RNA elongation; negative regulation of RNA elongation; negative regulation of transcription from RNA polymerase II promoter; cell cycle; chromatin remodeling; response to organic substance; retroviral genome replication; mRNA capping; positive regulation of RNA elongation from RNA polymerase II promoter; RNA elongation; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macroautophagy
Reference #:  O00267 (UniProtKB)
Alt. Names/Synonyms: DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; FLJ34157; hSPT5; SPT5; SPT5H; suppressor of Ty 5 homolog (S. cerevisiae); SUPT5H; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; Transcription elongation factor SPT5
Gene Symbols: SUPT5H
Molecular weight: 121,000 Da
Basal Isoelectric point: 4.95  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SPT5
Protein Structure Not Found.


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Sites Implicated In
transcription, altered: T768‑p, T775‑p, T784‑p, T791‑p, T799‑p, T806‑p, T814‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 S2 ______MSDSEDSNF
0 1 S4 ____MSDSEDSNFSE
0 1 S7 _MSDSEDSNFSEEED
0 1 S19-p EEDSERSsDGEEAEV
0 9 S32-p EVDEERRsAAGsEKE
0 9 S36-p ERRsAAGsEKEEEPE
0 1 Y86-p EADVDDEyEDEDQWE
0 107 Y140-p REEELGEyyMkKYAK
0 3 Y141-p EEELGEyyMkKYAKS
0 1 K143-u ELGEyyMkKYAKSSV
0 1 K199-u TAISLMRkFIAYQFT
0 1 T208-p IAYQFTDtPLQIkSV
0 2 K213-u TDtPLQIkSVVAPEH
0 1 Y230-p GYIYVEAyKQTHVKQ
0 1 K258-u NQQMVPIkEMTDVLK
0 3 Y295-p DDIAQVDyVEPSQNT
0 2 K317-a RIDYDRIkARMSLkD
0 1 K323-a IkARMSLkDWFAkRK
0 1 K323-u IkARMSLkDWFAkRK
0 2 K328-a SLkDWFAkRKKFKRP
0 1 S375-p RKGFLFKsFAMSAVI
0 6 K650-u LVLAGGSkPRDVTNF
0 2 A663 NFTVGGFAPMsPRIS
3 41 S666-p VGGFAPMsPRISsPM
0 4 S671-p PMsPRISsPMHPSAG
1 1 R681-m2 HPSAGGQrGGFGSPG
0 6 R681-m1 HPSAGGQrGGFGSPG
0 17 R696-m1 GGSGGMSrGrGRRDN
1 0 R696-m2 GGSGGMSrGrGRRDN
1 0 R698-m2 SGGMSrGrGRRDNEL
0 9 K718-u RISQGPYkGYIGVVK
0 1 K725 kGYIGVVKDATESTA
0 6 T762-p SRRPGGMtStyGRtP
0 1 T764-p RPGGMtStyGRtPMy
0 25 Y765-p PGGMtStyGRtPMyG
1 13 T768-p MtStyGRtPMyGsQt
0 41 Y771-p tyGRtPMyGsQtPMy
0 6 S773-p GRtPMyGsQtPMyGs
2 38 T775-p tPMyGsQtPMyGsGs
0 42 Y778-p yGsQtPMyGsGsRtP
0 2 S780-p sQtPMyGsGsRtPMy
0 2 S782-p tPMyGsGsRtPMyGs
1 10 T784-p MyGsGsRtPMyGsQt
0 44 Y787-p sGsRtPMyGsQtPLQ
0 13 S789-p sRtPMyGsQtPLQDG
2 52 T791-p tPMyGsQtPLQDGsR
0 9 S797-p QtPLQDGsRtPHyGs
1 26 T799-p PLQDGsRtPHyGsQt
0 24 Y802-p DGsRtPHyGsQtPLH
0 16 S804-p sRtPHyGsQtPLHDG
1 39 T806-p tPHyGsQtPLHDGsR
0 2 S812-p QtPLHDGsRtPAQSG
1 2 T814-p PLHDGsRtPAQSGAW
0 44 T1034-p SEHLEPItPtkNNKV
0 26 T1036-p HLEPItPtkNNKVKV
0 2 K1037-u LEPItPtkNNKVKVI
  mouse

 
S2-p ______MsDsEDsNF
S4-p ____MsDsEDsNFSE
S7-p _MsDsEDsNFSEEED
S19 EEDSERSSEAEEAEV
S32-p EVEEDQRsAAGsEKE
S36-p DQRsAAGsEKEEEPE
Y84 EADVDDEYEDEDQWE
Y138 REEELGEYYMKKYAK
Y139 EEELGEYYMKKYAKS
K141 ELGEYYMKKYAKSSV
K197 TAISLMRKFIAYQFT
T206 IAYQFTDTPLQIkSV
K211-u TDTPLQIkSVVAPEH
Y228 GYIYVEAYKQTHVKQ
K256 NQQMVPIKEMTDVLK
Y293-p DDIAQVDyVEPSQNT
K315 RIDYDRIKARMSLKD
K321 IKARMSLKDWFAKRK
K321 IKARMSLKDWFAKRK
K326 SLKDWFAKRKKFKRP
S373 RKGFLFKSFAMSAVI
K648-u LVLAGGSkPRDVTNL
T661-p NLTVGGFtPMsPRIS
S664-p VGGFtPMsPRISsPM
S669-p PMsPRISsPMHPSAE
H679 HPSAEGQHGGFGSPG
H679 HPSAEGQHGGFGSPG
R690-m1 GSPGGMSrGRGRRDN
R690 GSPGGMSRGRGRRDN
R692 PGGMSrGRGRRDNEL
K712-u RISQGPYkGYIGVVk
K719-u kGYIGVVkDATESTA
T756 SQRPGGMTSTYGRTP
T758 RPGGMTSTYGRTPMY
Y759 PGGMTSTYGRTPMYG
T762 MTSTYGRTPMYGSQT
Y765 TYGRTPMYGSQTPMY
S767 GRTPMYGSQTPMYGS
T769 TPMYGSQTPMYGSGS
Y772 YGSQTPMYGSGSRTP
S774 SQTPMYGSGSRTPMY
S776 TPMYGSGSRTPMYGS
T778 MYGSGSRTPMYGSQT
Y781 SGSRTPMYGSQTPLQ
S783 SRTPMYGSQTPLQDG
T785 TPMYGSQTPLQDGSR
S791 QTPLQDGSRtPHyGs
T793-p PLQDGSRtPHyGsQt
Y796-p DGSRtPHyGsQtPLH
S798-p SRtPHyGsQtPLHDG
T800-p tPHyGsQtPLHDGSR
S806 QtPLHDGSRTPAQSG
T808 PLHDGSRTPAQSGAW
T1028-p SEHLEPItPtkNNKV
T1030-p HLEPItPtkNNKVKV
K1031-u LEPItPtkNNKVKVI
  rat

 
S2 ______MSDSEDSNF
S4 ____MSDSEDSNFSE
S7 _MSDSEDSNFSEEED
S19 EEDSERSSEAEEAEV
S32 EVEEDRRSAAGSEKE
S36 DRRSAAGSEKEEEPE
Y85 EADVDDEYEDEDQWE
Y139 REEELGEYYMKKYAK
Y140 EEELGEYYMKKYAKS
K142 ELGEYYMKKYAKSSV
K198 TAISLMRKFIAYQFT
T207 IAYQFTDTPLQIKSV
K212 TDTPLQIKSVVAPEH
Y229 GYIYVEAYKQTHVKQ
K257 NQQMVPIKEMTDVLK
Y294 DDIAQVDYVEPSQNT
K316 RIDYDRIKARMSLKD
K322 IKARMSLKDWFAKRK
K322 IKARMSLKDWFAKRK
K327 SLKDWFAKRKKFKRP
S374 RKGFLFKSFAMSAVI
K649 LVLAGGSKPRDVTNL
T662 NLTVGGFTPMSPRIS
S665 VGGFTPMSPRISSPM
S670 PMSPRISSPMHPSAE
H680 HPSAEGQHGGFGSPG
H680 HPSAEGQHGGFGSPG
R691-m1 GSPGGMSrGRGRRDN
R691 GSPGGMSRGRGRRDN
R693 PGGMSrGRGRRDNEL
K713 RISQGPYKGYIGVVK
K720 KGYIGVVKDATESTA
T757 SQRPGGMTSTYGRTP
T759 RPGGMTSTYGRTPMy
Y760 PGGMTSTYGRTPMyG
T763 MTSTYGRTPMyGSQT
Y766-p TYGRTPMyGSQTPMy
S768 GRTPMyGSQTPMyGS
T770 TPMyGSQTPMyGSGS
Y773-p yGSQTPMyGSGSRTP
S775 SQTPMyGSGSRTPMY
S777 TPMyGSGSRTPMYGS
T779 MyGSGSRTPMYGSQT
Y782 SGSRTPMYGSQTPLQ
S784 SRTPMYGSQTPLQDG
T786 TPMYGSQTPLQDGSR
S792 QTPLQDGSRTPHYGS
T794 PLQDGSRTPHYGSQT
Y797 DGSRTPHYGSQTPLH
S799 SRTPHYGSQTPLHDG
T801 TPHYGSQTPLHDGSR
S807 QTPLHDGSRTPAQSG
T809 PLHDGSRTPAQSGAW
T1029 SEHLEPITPTKNNKV
T1031 HLEPITPTKNNKVKV
K1032 LEPITPTKNNKVKVI
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