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Protein Page:
Rad50 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Rad50 a component of the Mre11-Rad50-NBS1 (MRN) complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Three alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.-.-; Adaptor/scaffold; DNA repair, damage; Hydrolase
Cellular Component: nucleoplasm; Mre11 complex; nuclear chromosome, telomeric region; pronucleus; nucleolus; nucleus
Molecular Function: protein binding, bridging; ATP-dependent DNA helicase activity; protein binding; DNA binding; zinc ion binding; single-stranded DNA specific endodeoxyribonuclease activity; 3'-5' exonuclease activity; ATP binding
Biological Process: positive regulation of kinase activity; telomere maintenance via telomerase; DNA repair; regulation of mitotic recombination; DNA catabolic process, endonucleolytic; positive regulation of protein amino acid autophosphorylation; double-strand break repair via homologous recombination; DNA duplex unwinding; DNA recombination; double-strand break repair; meiotic recombination; response to DNA damage stimulus; telomere maintenance
Reference #:  Q92878 (UniProtKB)
Alt. Names/Synonyms: DNA repair protein RAD50; hRAD50; NBSLD; RAD50; RAD50 homolog (S. cerevisiae); RAD50-2
Gene Symbols: RAD50
Molecular weight: 153,892 Da
Basal Isoelectric point: 6.47  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Rad50

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K20-m1 RSFGIEDkDKQIITF
0 1 K62-u GDFPPGTkGNTFVHD
0 1 K175-u EGKALKQkFDEIFSA
0 1 K187-u FSATRYIkALETLRQ
0 1 K211-u KEYQMELkYLKQYKE
0 1 K230-u IRDQITSkEAQLTSS
0 1 K242-u TSSKEIVkSYENELD
0 1 K264-a EIEHNLSkIMKLDNE
0 1 K295-u ELEEKMEkVFQGTDE
0 1 K461-u QNELKNVkYELQQLE
0 1 S560 RKIKSRHSDELTSLL
0 1 S565 RHSDELTSLLGYFPN
0 1 S603 KLNKELASSEQNKNH
0 15 S635-p KLFDVCGsQDFEsDL
0 1 S640-p CGsQDFEsDLDRLKE
0 36 T690-p VCQRVFQtEAELQEV
0 4 R797-m2 TDVTIMErFQMELKD
0 1 K845-u KLDTVSSkIELNRKL
0 2 K959-a KNIHGYMkDIENYIQ
0 1 T1113-p EMMIVMRttELVNkD
0 1 T1114-p MMIVMRttELVNkDL
0 1 K1119-u RttELVNkDLDIYyk
0 2 Y1125-p NkDLDIYykTLDQAI
0 1 K1126-u kDLDIYykTLDQAIM
0 1 K1134-u TLDQAIMkFHSMKME
0 2 K1190-u NYRVVMLkGDTALDM
0 1 K1285-a GRSEYVEkFYRIKKN
0 1 K1285-u GRSEYVEkFYRIKKN
  mouse

 
K20 RSFGIEDKDKQIISF
K62 GDFPPGTKGNTFVHD
K175 EGKALKQKFDEIFSA
K187 FSATRYIKALDTLRQ
K211 KECQTELKYLKQNKE
K230 IRDQITSKEAQLASS
R242 ASSQEIVRSYEDELE
K264 EIEHNLSKIMKLDNE
K295 ELEQKMEKVFQGTDE
R461 QSELRHVRSELQQLE
S560-p RKIKSRHsDELTsLL
S565-p RHsDELTsLLGYFPN
S603-p KLNKELAsAEQNKNH
S635-p KLFDVCGsQDLESDL
S640 CGsQDLESDLGRLKE
T690-p VCQRVFQtEAELQEV
R797-m2 TDVTIMErFQMELKD
K845 RLDTVTSKIELNRKL
K959 KNIHGYMKDIENYIQ
T1113 EMMIVMRTTELVNKD
T1114 MMIVMRTTELVNKDL
K1119 RTTELVNKDLDIYYK
Y1125 NKDLDIYYKTLDQAI
K1126 KDLDIYYKTLDQAIM
K1134 TLDQAIMKFHSMKME
K1190 NYRVVMLKGDTALDM
K1285 GRSEYVEKFYRVKKN
K1285 GRSEYVEKFYRVKKN
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