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Protein Page:
Rad50 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Rad50 a component of the Mre11-Rad50-NBS1 (MRN) complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Three alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.-.-; Adaptor/scaffold; Hydrolase; DNA repair, damage
Cellular Component: nucleoplasm; Mre11 complex; nuclear chromosome, telomeric region; membrane; pronucleus; nucleolus; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein binding, bridging; protein binding; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; zinc ion binding; 3'-5' exonuclease activity; ATP binding
Biological Process: positive regulation of kinase activity; telomere maintenance via telomerase; regulation of mitotic recombination; DNA repair; DNA catabolic process, endonucleolytic; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; double-strand break repair via homologous recombination; DNA recombination; double-strand break repair; meiotic recombination; telomere maintenance; response to DNA damage stimulus
Reference #:  Q92878 (UniProtKB)
Alt. Names/Synonyms: DNA repair protein RAD50; hRAD50; NBSLD; RAD50; RAD50 homolog (S. cerevisiae); RAD50-2
Gene Symbols: RAD50
Molecular weight: 153,892 Da
Basal Isoelectric point: 6.47  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Rad50

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K20-m1 RSFGIEDkDKQIITF
0 1 T54 ECLKYICTGDFPPGT
0 1 T61 TGDFPPGTkGNTFVH
0 1 K62-ub GDFPPGTkGNTFVHD
0 1 T119 KTLEGVITRTKHGEK
0 1 S128-p TKHGEKVsLsSKCAE
0 1 S130-p HGEKVsLsSKCAEID
0 1 K175-ub EGKALKQkFDEIFSA
0 1 K187-ub FSATRYIkALETLRQ
0 1 T198-p TLRQVRQtQGQKVKE
0 1 K211-ub KEYQMELkYLKQYKE
0 1 K230-ub IRDQITSkEAQLTSS
0 1 K242-ub TSSKEIVkSYENELD
0 1 K264-ac EIEHNLSkIMKLDNE
0 1 K284 SRKKQMEKDNSELEE
0 1 K295-ub ELEEKMEkVFQGTDE
0 1 K331 DCHRELEKLNKESRL
0 1 K398-ac RQIKNFHkLVRERQE
0 1 K453-ac KSEILSKkQNELKNV
0 1 K461-ac QNELKNVkYELQQLE
0 1 K461-ub QNELKNVkYELQQLE
0 1 S560 RKIKSRHSDELTSLL
0 1 S565 RHSDELTSLLGYFPN
0 1 S603 KLNKELASSEQNKNH
0 17 S635-p KLFDVCGsQDFEsDL
0 1 S640-p CGsQDFEsDLDRLKE
0 34 T690-p VCQRVFQtEAELQEV
0 1 K704 VISDLQSKLRLAPDK
0 4 R797-m2 TDVTIMErFQMELKD
0 1 K845-ub KLDTVSSkIELNRKL
0 1 K950 KLNDIKEKVKNIHGY
0 3 K959-ac KNIHGYMkDIENYIQ
0 1 K1032-ac RKRNEELkEVEEERK
0 1 T1113-p EMMIVMRttELVNkD
0 1 T1114-p MMIVMRttELVNkDL
0 1 K1119-ub RttELVNkDLDIYyk
0 2 Y1125-p NkDLDIYykTLDQAI
0 1 K1126-ub kDLDIYykTLDQAIM
0 1 K1134-ub TLDQAIMkFHSMKME
0 2 K1190-ub NYRVVMLkGDTALDM
0 2 K1285-ac GRSEYVEkFYRIKKN
0 1 K1285-ub GRSEYVEkFYRIKKN
14223 : Phospho-Rad50 (Ser635) Antibody
  mouse

 
K20 RSFGIEDKDKQIISF
T54-p ECLKYICtGDFPPGt
T61-p tGDFPPGtKGNTFVH
K62 GDFPPGtKGNTFVHD
T119-p KTLEGVItRMKHGEK
S128 MKHGEKVSLSSKCAE
S130 HGEKVSLSSKCAEID
K175 EGKALKQKFDEIFSA
K187 FSATRYIKALDTLRQ
T198 TLRQVRQTQGQKVKE
K211 KECQTELKYLKQNKE
K230 IRDQITSKEAQLASS
R242 ASSQEIVRSYEDELE
K264 EIEHNLSKIMKLDNE
K284-ub SRKKQMEkDNSELEQ
K295 ELEQKMEKVFQGTDE
K331-ac DCQRELEkLNKEARL
E398 RQIKNFHELVKERQE
K453 KTEILTKKQSELRHV
R461 QSELRHVRSELQQLE
R461 QSELRHVRSELQQLE
S560-p RKIKSRHsDELTsLL
S565-p RHsDELTsLLGYFPN
S603-p KLNKELAsAEQNKNH
S635-p KLFDVCGsQDLESDL
S640 CGsQDLESDLGRLKE
T690-p VCQRVFQtEAELQEV
K704-ub VISDLQSkLRLAPDK
R797-m2 TDVTIMErFQMELKD
K845 RLDTVTSKIELNRKL
K950-ac KINDIKEkVKNIHGY
K959 KNIHGYMKDIENYIQ
K1032 RKRRDELKEVEEEPK
T1113 EMMIVMRTTELVNKD
T1114 MMIVMRTTELVNKDL
K1119 RTTELVNKDLDIYYK
Y1125 NKDLDIYYKTLDQAI
K1126 KDLDIYYKTLDQAIM
K1134 TLDQAIMKFHSMKME
K1190 NYRVVMLKGDTALDM
K1285 GRSEYVEKFYRVKKN
K1285 GRSEYVEKFYRVKKN
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