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Protein Page:
ENPP1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ENPP1 Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Note: This description may include information from UniProtKB.
Protein type: Phosphodiesterase; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.1.4.1; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Carbohydrate Metabolism - starch and sucrose; Nucleotide Metabolism - purine; EC 3.6.1.9; Cofactor and Vitamin Metabolism - riboflavin; Phosphatase (non-protein)
Cellular Component: extracellular space; cell surface; lysosomal membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane; integral to membrane
Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; protein homodimerization activity; 3'-phosphoadenosine 5'-phosphosulfate binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; insulin receptor binding; polysaccharide binding; protein binding; nucleic acid binding; scavenger receptor activity; ATP binding
Biological Process: ATP catabolic process; receptor-mediated endocytosis; generation of precursor metabolites and energy; sequestering of triacylglycerol; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; negative regulation of protein amino acid autophosphorylation; immune response; negative regulation of cell growth; regulation of bone mineralization; inorganic diphosphate transport; water-soluble vitamin metabolic process; negative regulation of glycogen biosynthetic process
Reference #:  P22413 (UniProtKB)
Alt. Names/Synonyms: alkaline phosphodiesterase 1; Alkaline phosphodiesterase I; ARHR2; E-NPP 1; ectonucleotide pyrophosphatase/phosphodiesterase 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ENPP1; Ly-41 antigen; M6S1; Membrane component chromosome 6 surface marker 1; membrane component, chromosome 6, surface marker 1; NPP1; NPPase; NPPS; Nucleotide pyrophosphatase; PC-1; PC1; PCA1; PDNP1; Phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; Plasma-cell membrane glycoprotein PC-1
Gene Symbols: ENPP1
Molecular weight: 104,924 Da
Basal Isoelectric point: 6.76  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ENPP1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 3 P43 AAEAPGDPQAAASLL
0 1 K62-ac VGEEPLEkAArARTA
0 1 R65-m1 EPLEkAArARTAKDP
0 1 T74 RTAKDPNTYKVLSLV
0 1 Y75 TAKDPNTYKVLSLVL
0 1 T88 VLSVCVLTTILGCIF
0 1 T89 LSVCVLTTILGCIFG
1 4 T256-p RPVYPTKtFPNHYSI
0 2 Y280-p GIIDNKMyDPKMNAS
0 1 Y440-p KYIYLNKyLGDVKNI
  mouse

 
S25-p GNGRELEsPAAASLL
K44 LGEEPLEKAERARPA
R47 EPLEKAERARPAKDP
T56 RPAKDPNTYKVLSLV
Y57 PAKDPNTYKVLSLVL
T70 VLSVCVLTTILGCIF
T71 LSVCVLTTILGCIFG
T238-p RPMYPTKtFPNHYSI
Y262 GIIDNKMYDPKMNAS
Y422 KYVYLNKYLGDVNNV
  rat

 
S25-p GNGRELEsPAAASLL
K44 LGEEPLEKAERARTA
R47 EPLEKAERARTAKDP
T56-p RTAKDPNtyKVLSLV
Y57-p TAKDPNtyKVLSLVL
T70-p VLSVCVLttILGCIF
T71-p LSVCVLttILGCIFG
T238-p RPVYPTKtFPNHYSI
Y262 GIIDNKMYDPKMNAS
Y422 KYVYLNKYLGDVNNV
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