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Protein Page:
JNK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
JNK1 a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily
Cellular Component: nucleoplasm; mitochondrion; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; histone deacetylase regulator activity; histone deacetylase binding; JUN kinase activity; ATP binding
Biological Process: ossification; regulation of histone deacetylation; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; peptidyl-threonine phosphorylation; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; peptidyl-serine phosphorylation; regulation of protein localization; response to cadmium ion; regulation of transcription factor activity; toll-like receptor signaling pathway; innate immune response; response to stress; JNK cascade; toll-like receptor 9 signaling pathway; negative regulation of protein binding; toll-like receptor 4 signaling pathway; response to UV; negative regulation of apoptosis
Reference #:  P45983 (UniProtKB)
Alt. Names/Synonyms: c-Jun N-terminal kinase 1; JNK; JNK-46; JNK1; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1A2; JNK21B1/2; JUN N-terminal kinase; MAP kinase 8; MAPK 8; MAPK8; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; MK08; PRKM8; protein kinase JNK1; SAPK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1
Gene Symbols: MAPK8
Molecular weight: 48,296 Da
Basal Isoelectric point: 6.43  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  ErbB/HER Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Mitochondrial Control of Apoptosis  |  NF-kB Signaling  |  Parkinson's Disease  |  SAPK/JNK Signaling Cascades  |  T Cell Receptor Signaling  |  TGF-├č Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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JNK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: T183‑p, Y185‑p
apoptosis, induced: T183‑p, Y185‑p
cell adhesion, altered: T183‑p, Y185‑p
transcription, altered: T183‑p, Y185‑p
enzymatic activity, induced: T183‑p, Y185‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 S129 ELDHERMSYLLYQML
0 3 K166-ub VKSDCTLkILDFGLA
1 2 T178-p GLARTAGtsFMMtPy
0 8 S179-p LARTAGtsFMMtPyV
205 389 T183-p AGtsFMMtPyVVtRY
204 1234 Y185-p tsFMMtPyVVtRYYR
0 24 T188-p MMtPyVVtRYYRAPE
1 0 R228 HKILFPGRDYIDQWN
1 0 S284 DVLFPADSEHNKLKA
0 1 K300-ub QARDLLSkMLVIDAS
0 20 K308-ac MLVIDASkRISVDEA
1 10 S377 GVIRGQPSPLGAAVI
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
4668 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4671 : Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
9257 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor(R) 647 Conjugate)
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
4668 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4671 : Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
9257 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor(R) 647 Conjugate)
  JNK1 iso2  
S129 ELDHERMSYLLYQML
K166 VKSDCTLKILDFGLA
T178 GLARTAGTSFMMtPy
S179 LARTAGTSFMMtPyV
T183-p AGTSFMMtPyVVTRY
Y185-p TSFMMtPyVVTRYYR
T188 MMtPyVVTRYYRAPE
R228 HKILFPGRDYIDQWN
S284 DVLFPADSEHNKLKA
K300 QARDLLSKMLVIDAS
K308-ac MLVIDASkRISVDEA
S377-p GVIRGQPsPLAQVQQ
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
  JNK1 iso3  
S129 ELDHERMSYLLYQML
K166 VKSDCTLKILDFGLA
T178 GLARTAGTSFMMtPy
S179 LARTAGTSFMMtPyV
T183-p AGTSFMMtPyVVTRY
Y185-p TSFMMtPyVVTRYYR
T188 MMtPyVVTRYYRAPE
T228-p GGVLFPGtDHIDQWN
S284-p DVLFPADsEHNKLKA
K300 QARDLLSKMLVIDAS
K308 MLVIDASKRISVDEA
S377-p GVIRGQPsPLAQVQQ
  mouse

 
S129-p ELDHERMsYLLYQML
K166-ub VKSDCTLkILDFGLA
T178-p GLARTAGtsFMMtPy
S179-p LARTAGtsFMMtPyV
T183-p AGtsFMMtPyVVtRY
Y185-p tsFMMtPyVVtRYYR
T188-p MMtPyVVtRYYRAPE
R228 HKILFPGRDYIDQWN
S284 DVLFPADSEHNKLKA
K300 QARDLLSKMLVIDAS
K308-ac MLVIDASkRISVDEA
S377-p GVIRGQPsPLAQVQQ
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
4668 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4671 : Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
9257 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor(R) 647 Conjugate)
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
4668 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4671 : Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
9257 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor(R) 647 Conjugate)
  rat

 
S129 ELDHERMSYLLYQML
K166 VKSDCTLKILDFGLA
T178 GLARTAGTsFMMtPy
S179-p LARTAGTsFMMtPyV
T183-p AGTsFMMtPyVVtRY
Y185-p TsFMMtPyVVtRYYR
T188-p MMtPyVVtRYYRAPE
R228 LKILFPGRDYIDQWN
S284 DVLFPADSEHNKLKA
K300 QARDLLSKMLVIDAS
K308 MLVIDASKRISVDEA
S377 GVIRGQPSPLGAAVI
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
4668 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4671 : Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
9257 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor(R) 647 Conjugate)
4306 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)
4668 : Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4671 : Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
5136 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated)
5755 : Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)
9251 : Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9255 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
9257 : Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor(R) 647 Conjugate)
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