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Protein Page:
USP9X (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
USP9X Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Interacts with SMAD4, MARK4, NUAK1 and BIRC5/survivin. Widely expressed in embryonic and adult tissues. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin conjugating system; Ubiquitin-specific protease; Protease; EC 3.4.19.12; EC 3.1.2.15
Chromosomal Location of Human Ortholog: Xp11.4
Cellular Component: growth cone; membrane; apical part of cell; cytoplasm; cytosol
Molecular Function: ubiquitin thiolesterase activity; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity
Biological Process: ubiquitin-dependent protein catabolic process; mitosis; transcription initiation from RNA polymerase II promoter; protein deubiquitination; transcription, DNA-dependent; axon extension; neuron migration; female gamete generation; negative regulation of transcription from RNA polymerase II promoter; chromosome segregation; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; gene expression
Reference #:  Q93008 (UniProtKB)
Alt. Names/Synonyms: Deubiquitinating enzyme FAF-X; DFFRX; Drosophila fat facets related, X-linked; FAF; FAM; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; hFAM; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; ubiquitin specific peptidase 9, X-linked; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X; USP9; USP9X
Gene Symbols: USP9X
Molecular weight: 292,280 Da
Basal Isoelectric point: 5.52  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

USP9X

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S199-p PCESVSSsVQLPEDE
0 1 K218-ub SPDPRSPkGWLVDLL
0 1 K303-ac KEAKNEAkNDALSMI
0 1 K303 KEAKNEAKNDALSMI
0 1 K315-ac SMIIKSLkNLASRVP
0 2 K315-ub SMIIKSLkNLASRVP
0 1 K329-ub PGQEETVkNLEIFRL
0 4 Y367-p KVISSVSyYTHRHGN
0 1 N374 yYTHRHGNPEEEEWL
0 1 P375 YTHRHGNPEEEEWLT
0 1 K412 HQPQYVEKLEKILRF
0 14 S588-p GEAPQNLsQtQRsPH
0 21 T590-p APQNLsQtQRsPHVF
0 4 S593-p NLsQtQRsPHVFYRH
0 1 K745 KCFERFFKAVNCREG
0 1 K837 LCVLDGDKDSVNCAr
0 1 R844-m1 KDSVNCArQEAVRMV
0 1 Y860 VLTVLREYINECDSD
0 1 Y868-p INECDSDyHEERTIL
0 1 S887-p AFRGKHLsFVVRFPN
0 1 T930-p IKANVAHtKIELFVG
0 1 T984-p DSSSDSStGSPGNHG
0 2 K1045-ub DGARVLMkLMPPDST
0 9 K1066-ub AICLDHAkLGESSLS
0 1 S1226 VRLAQQISDEASRYM
0 2 K1370-ub STARERAkHSGDYFT
0 1 K1435 EGHLGVTKELLAFQT
0 1 K1453 KFHIGCEKGGANLIK
0 2 T1591-p GILAIEGtGsDVDDD
0 9 S1593-p LAIEGtGsDVDDDMs
0 27 S1600-p sDVDDDMsGDEKQDN
0 9 S1609-p DEKQDNEsNVDPRDD
0 1 Y1620-p PRDDVFGyPQQFEDk
0 43 K1627-ub yPQQFEDkPALSkTE
0 6 K1632-ub EDkPALSkTEDRkEY
0 2 K1637-ub LSkTEDRkEYNIGVL
0 2 K1669-ub YVPRGFWkQFRLWGE
0 2 K1712-ub GHPAMLSkVLGGSFA
0 2 K1798-ub PVLAIQLkRFDYDWE
0 2 K1811-ub WERECAIkFNDYFEF
0 2 Y1815 CAIkFNDYFEFPREL
0 1 K1862-ub SETAGSTkYRLVGVL
0 1 K1899 GERNRWYKFDDGDVT
0 1 S2109 EYLLECPSAEVRGAF
0 1 Y2185 YFNLFVMYANLGVAE
0 1 T2194 NLGVAEKTQLLKLSV
0 4 K2219-ub EGPGPPIkYQYAELG
0 1 S2242 LIRCCNVSSRMQSSI
0 1 S2243 IRCCNVSSRMQSSIN
0 1 S2247 NVSSRMQSSINGNPP
0 1 S2248 VSSRMQSSINGNPPL
0 1 P2254 SSINGNPPLPNPFGD
0 1 S2265 PFGDPNLSQPIMPIQ
0 1 K2288 VRTSYVKKIIEDCSN
0 1 K2301-ub SNSEETVkLLRFCCW
0 2 K2360-ub HRIHNALkGIPDDRD
0 1 Y2431-p DELERRPyTGNPQyt
0 1 T2432 ELERRPyTGNPQyty
0 1 Y2437-p PyTGNPQytyNNWsP
0 2 T2438-p yTGNPQytyNNWsPP
0 5 Y2439-p TGNPQytyNNWsPPV
0 22 S2443-p QytyNNWsPPVQsNE
0 2 S2448-p NWsPPVQsNETSNGy
0 2 Y2455-p sNETSNGyFLERSHS
0 140 Y2556-p GQRAQENyEGsEEVs
0 1 S2559-p AQENyEGsEEVsPPQ
0 14 S2563-p yEGsEEVsPPQtKDQ
0 1 T2567-p EEVsPPQtKDQ____
  mouse

 
S199 PCESVSSSVQLPEDE
K218 SPDPRSPKGWLVDLL
K303 KEAKNEAKNDALSMI
K303-ub KEAKNEAkNDALSMI
K315 SMIIKSLKNLASRVP
K315-ub SMIIKSLkNLASRVP
K329 PGQEETVKNLEIFRL
Y367-p KVISSVSyYTHRHGs
S374-p yYTHRHGssEDEEWL
S375-p YTHRHGssEDEEWLT
K412-ub HQPQYVEkLEKILRF
S588-p GEAPQNLsQsQRsPH
S590-p APQNLsQsQRsPHVF
S593-p NLsQsQRsPHVFYRH
K745-ub KCFERFFkAVNCREG
K837-ub LCVLDGDkDSINCAR
R844 kDSINCARQEAVRMV
Y860-p VLTVLREyINECDSD
Y868 INECDSDYHEERTIL
S887-p AFRGKHLsFIVRFPN
T930 IKANVAHTKIELFVG
T984 DSSSDSSTGSPGNHG
K1045-ub DGARVLMkLMPPDST
K1066-ub AICLDHAkLGESSLS
S1226-p VRLAQQIsDEASRYM
K1370-ub STARERAkHSGDYFT
K1435-ub EGHLGVTkELLAFQT
K1453-ub KFHIGCEkGGANLIK
T1591-p GILAIEGtGsDVDDD
S1593-p LAIEGtGsDVDDDMs
S1600-p sDVDDDMsGDEKQDN
S1609-p DEKQDNEsNVDPRDD
Y1620 PRDDVFGYPQQFEDk
K1627-ub YPQQFEDkPPLSkTE
K1632-ub EDkPPLSkTEDRkEY
K1637-ub LSkTEDRkEYNIGVL
K1669-ub YVPRGFWkQFRLWGE
K1712-ub GHPAMLSkVLGGSFA
K1798-ub PVLAIQLkRFDYDWE
K1811-ub WERECAIkFNDyFEF
Y1815-p CAIkFNDyFEFPREL
K1862 SEKAGSTKYRLVGVL
K1899-ub GEKNRWYkFDDGDVT
S2109-p EYLLECPsAEVRGAF
Y2185-p YFNLFVMyANLGVAE
T2194-p NLGVAEKtQLLKLSV
K2219-ub EGPGPPIkYQYAELG
S2242-p LIRCCNVssRMQssI
S2243-p IRCCNVssRMQssIN
S2247-p NVssRMQssINGNPs
S2248-p VssRMQssINGNPsL
S2254-p ssINGNPsLPNPFGD
S2265-p PFGDPNLsQPIMPIQ
K2288-ub VRTSYVKkIIEDCSN
K2301 SNSDETVKLLRFCCW
K2360-ub HRIHNALkGIPDDRD
Y2431-p DELERRPytGNPQyt
T2432-p ELERRPytGNPQyty
Y2437-p PytGNPQytyNNWsP
T2438-p ytGNPQytyNNWsPP
Y2439-p tGNPQytyNNWsPPV
S2443-p QytyNNWsPPVQsNE
S2448-p NWsPPVQsNETSNGy
Y2455-p sNETSNGyFLERSHS
Y2540 GQRAQENYEGGEEVs
G2543 AQENYEGGEEVsPPQ
S2547-p YEGGEEVsPPQTKGS
T2551 EEVsPPQTKGSVKCT
  rat

 
S199 PCESVSSSVQLPEDE
K218 SPDPRSPKGWLVDLL
K303 KEAKNEAKNDALSMI
K303 KEAKNEAKNDALSMI
K315 SMIIKSLKSLASRVP
K315 SMIIKSLKSLASRVP
K329 PGQEETVKNLEIFRL
Y367 KVISSVSYYTHRHGs
S374-p YYTHRHGssEEEEWL
S375-p YTHRHGssEEEEWLT
K412 HQPQYVEKLEKILRF
S588 GEAPQNLSQTQRSPH
T590 APQNLSQTQRSPHVF
S593 NLSQTQRSPHVFYRH
K745 KCFERFFKAVNCREG
K837 LCVLDGDKDSINCAR
R844 KDSINCARQEAVRMV
Y860 VLTVLREYINECDSD
Y868 INECDSDYHEERTIL
S887 AFRGKHLSFIVRFPN
T930 IKANVAHTKIELFVG
T984 DSSSDSSTGSPGNHG
K1045 DGARVLMKLMPPDST
K1066 AICLDHAKLGESSLS
S1226 VRLAQQISDEASRYM
K1370 STARERAKHSGDYFT
K1435 EGHLGVTKELLAFQT
K1453 KFHIGCEKGGANLIK
T1591 GILAIEGTGsDVDDD
S1593-p LAIEGTGsDVDDDMs
S1600-p sDVDDDMsGDEKQDN
S1609 DEKQDNESNVDPRDD
Y1620 PRDDVFGYPQQFEDk
K1627-ub YPQQFEDkPPLSKTE
K1632 EDkPPLSKTEDRKEY
K1637 LSKTEDRKEYNIGVL
K1669 YVPRGFWKQFRLWGE
K1712 GHPAMLSKVLGGSFA
K1798 PVLAIQLKRFDYDWE
K1811 WERECAIKFNDYFEF
Y1815 CAIKFNDYFEFPREL
K1862 SEKAGSTKYRLVGVL
K1899 GEKNRWYKFDDGDVT
S2109 EYLLECPSAEVRGAF
Y2185 YFNLFVMYANLGVAE
T2194 NLGVAEKTQLLKLSV
K2219 EGPGPPIKYQYAELG
S2242 LIRCCNVSSRMQSSI
S2243 IRCCNVSSRMQSSIN
S2247 NVSSRMQSSINGNPS
S2248 VSSRMQSSINGNPSL
S2254 SSINGNPSLPNPFGD
S2265 PFGDPNLSQPIMPIQ
K2288 VRTSYVKKIIEDCSN
K2301 SNSDETVKLLRFCCW
K2360 HRIHNALKGIPDDRD
Y2431 DELERRPYTGNPQYT
T2432 ELERRPYTGNPQYTY
Y2437 PYTGNPQYTYNNWsP
T2438 YTGNPQYTYNNWsPP
Y2439 TGNPQYTYNNWsPPV
S2443-p QYTYNNWsPPVQSNE
S2448 NWsPPVQSNETSNGY
Y2455 SNETSNGYFLERSHS
Y2533 GQRAQENYEGSEEVS
S2536 AQENYEGSEEVSPPQ
S2540 YEGSEEVSPPQTKDQ
T2544 EEVSPPQTKDQ____
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