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PACT (human)

Overview PACT a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB. Protein type: RNA processing; Activator protein; RNA binding protein Cellular Component: perinuclear region of cytoplasm; cytoplasm Molecular Function: protein binding; protein homodimerization activity; double-stranded RNA binding; enzyme activator activity Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; induction of apoptosis; response to stress; immune response; middle ear morphogenesis; protein amino acid phosphorylation; RNA interference, production of siRNA Reference #:  O75569 (UniProtKB) Alt. Names/Synonyms: DYT16; HSD14; Interferon-inducible double stranded RNA-dependent protein kinase activator A; PACT; PKR-associated protein X; PKR-associating protein X; PRKRA; Protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA dependent activator; Protein kinase, interferon-inducible double stranded RNA-dependent activator; RAX Gene Symbols: PRKRA Molecular weight: 34,404 Da Basal Isoelectric point: 8.68  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PACT 2DIX - A=32-104 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  Phospho3D  |  DISEASE 603424 612067  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
apoptosis, altered: S246‑p, S287‑p

	Modification Sites and Domains

	Modification Sites in Parent Protein, Orthologs, and Isoforms

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
3	136		S18-p	PPLEREDsGtFsLGK
0	13		T20-p	LEREDsGtFsLGKMI
0	5		S22-p	REDsGtFsLGKMITA
0	1		T71-p	PTFTFRVtVGDITCT
0	4		S130-p	NQLNPIGsLQELAIH
0	1		Y159-p	GPAHKREytTICRLE
0	2		T160-p	PAHKREytTICRLEs
0	87		S167-p	tTICRLEsFMETGKG
0	1		S217-p	SLGCTWHsLRNSPGE
0	1		S235-p	LLKRSLLsIPNTDYI
3	1		S246-p	TDYIQLLsEIAKEQG
0	1		Y270-p	ELSANGQyQCLAELS
3	0		S287-p	PITVCHGsGISCGNA

	mouse
S18-p	PPLQREDsGtFsLGK
T20-p	LQREDsGtFsLGKMI
S22-p	REDsGtFsLGKMITA
T71	PTFTFRVTVGDITCT
S130-p	NQLNPIGsLQELAIH
Y159	GPAHKREYTTICRLE
T160	PAHKREYTTICRLEs
S167-p	TTICRLEsFMETGKG
S217	SLGCTWHSLRNSPGE
S235	LLKRSLLSLPNTDYI
S246	TDYIQLLSEIASEQG
Y270	ELSANGQYQCLAELS
S287	PITVCHGSGISCGNA

	rat
S18-p	PPLQREDsGTFSLGK
T20	LQREDsGTFSLGKMI
S22	REDsGTFSLGKMITA
T71	PTFTFRVTVGDITCT
S130	NQLNPIGSLQELAIH
Y159	GPAHKREYTTICRLE
T160	PAHKREYTTICRLES
S167	TTICRLESFMETGKG
S217	SLGCTWHSLRNSPGE
S235	LLKRSLLSLPNTDYI
S246	TDYIQLLSEIAKEQG
Y270	ELSANGQYQCLAELS
S287	PITVCHGSGISCGNA

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