Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
NSF (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
NSF required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.4.6; Vesicle protein; Adaptor/scaffold; Autophagy; Protease
Cellular Component: Golgi stack; cytoplasm; plasma membrane; dendritic shaft; cytosol
Molecular Function: protein C-terminus binding; protein binding; syntaxin binding; metal ion binding; protein complex binding; ATPase activity, coupled; ATP binding; Rab GTPase binding; PDZ domain binding
Biological Process: regulation of exocytosis; synaptic transmission; protein transport; exocytosis; positive regulation of receptor recycling; potassium ion transport; plasma membrane fusion
Reference #:  P46459 (UniProtKB)
Alt. Names/Synonyms: N-ethylmaleimide-sensitive factor; N-ethylmaleimide-sensitive factor-like protein; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; NSF; SKD2; Vesicle-fusing ATPase; Vesicular-fusion protein NSF
Gene Symbols: NSF
Molecular weight: 82,594 Da
Basal Isoelectric point: 6.52  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NSF
Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  ENZYME  |  DISEASE  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
enzymatic activity, induced: Y83‑p
molecular association, regulation: Y83‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 K27 NCAVVNEKDFQSGQH
0 1 Y45-p RTSPNHRyTFTLKTH
1 1 Y83-p QEIEVSLyTFDKAKQ
0 1 T94 KAKQCIGTMTIEIDF
0 1 K105 EIDFLQKKSIDSNPY
0 1 S109 LQKKSIDSNPYDTDK
0 6 K161 AMDPSILKGEPATGK
0 2 K168 KGEPATGKRQKIEVG
0 1 K198 SSLNLIGKAKTKENR
0 9 S207-p KTKENRQsIINPDWN
0 7 K217 NPDWNFEKMGIGGLD
0 6 K225 MGIGGLDKEFSDIFR
0 1 S228 GGLDKEFSDIFRRAF
0 6 K254 QMGCKHVKGILLyGP
0 40 Y259-p HVKGILLyGPPGCGk
0 13 K266-u yGPPGCGkTLLARQI
0 1 K275 LLARQIGKMLNAREP
0 3 K283 MLNAREPKVVNGPEI
0 4 K293 NGPEILNKYVGEsEA
0 2 Y294 GPEILNKYVGEsEAN
0 1 S298-p LNKYVGEsEANIRKL
0 1 K304 EsEANIRKLFADAEE
0 3 K335 DEIDAICKQRGsMAG
0 2 S339-p AICKQRGsMAGSTGV
0 1 S343 QRGsMAGSTGVHDtV
0 8 T349-p GSTGVHDtVVNQLLs
0 1 S356-p tVVNQLLsKIDGVEQ
0 1 K392 RPGRLEVKMEIGLPD
0 7 K401 EIGLPDEKGRLQILH
0 1 S421 MRGHQLLSADVDIKE
0 2 K427 LSADVDIKELAVETk
0 2 K434-a KELAVETkNFSGAEL
0 10 K434-u KELAVETkNFSGAEL
0 2 S437 AVETkNFSGAELEGL
0 1 S450 GLVRAAQSTAMNRHI
1 0 S460 MNRHIKASTKVEVDM
1 0 T461 NRHIKASTKVEVDME
0 3 K462 RHIKASTKVEVDMEk
0 11 K469-u KVEVDMEkAESLQVT
0 1 Y499-p FGTNQEDyASYIMNG
0 8 K529-u ELLVQQTkNSDRTPL
0 3 K549 EGPPHSGKTALAAkI
0 19 K555-u GKTALAAkIAEESNF
0 5 K566 ESNFPFIKICsPDKM
1 3 S569-p FPFIKICsPDKMIGF
0 3 K572 IKICsPDKMIGFSET
0 1 K581-a IGFSETAkCQAMKKI
0 2 K581 IGFSETAKCQAMKKI
0 5 K594 KIFDDAYKSQLSCVV
0 4 Y611 DIERLLDYVPIGPRF
0 1 K699-a TTIAQQVkGKKVWIG
0 8 K699 TTIAQQVKGKKVWIG
0 1 K702 AQQVkGKKVWIGIKK
0 1 K708 KKVWIGIKKLLMLIE
0 1 K709 KVWIGIKKLLMLIEM
0 5 S739-p LLREEGAsPLDFD__
  mouse

 
K27 NCAVVNEKDFQSGQH
Y45 RTSPNHKYIFTLRTH
Y83 QDIEVALYSFDKAKQ
T94-p KAKQCIGtMTIEIDF
K105-u EIDFLQKkNIDsNPY
S109-p LQKkNIDsNPYDTDK
K161-u AMDPSILkGEPASGK
K168 kGEPASGKRQKIEVG
K198-u SSLNLIGkAKTKENR
S207-p KTKENRQsIINPDWN
K217-u NPDWNFEkMGIGGLD
K225 MGIGGLDKEFsDIFR
S228-p GGLDKEFsDIFRRAF
K254 QMGCKHVKGILLyGP
Y259-p HVKGILLyGPPGCGk
K266-u yGPPGCGkTLLARQI
K275 LLARQIGKMLNAREP
K283 MLNAREPKVVNGPEI
K293-u NGPEILNkYVGESEA
Y294 GPEILNkYVGESEAN
S298 LNkYVGESEANIRkL
K304-u ESEANIRkLFADAEE
K335 DEIDAICKQRGsMAG
S339-p AICKQRGsMAGsTGV
S343-p QRGsMAGsTGVHDTV
T349 GsTGVHDTVVNQLLS
S356 TVVNQLLSKIDGVEQ
K392 RPGRLEVKMEIGLPD
K401-u EIGLPDEkGRLQILH
S421-p MRGHQLLsADVDIKE
K427 LsADVDIKELAVETk
K434 KELAVETKNFsGAEL
K434-u KELAVETkNFsGAEL
S437-p AVETkNFsGAELEGL
S450-p GLVRAAQsTAMNRHI
S460-p MNRHIKAstkVEVDM
T461-p NRHIKAstkVEVDME
K462-u RHIKAstkVEVDMEk
K469-u kVEVDMEkAESLQVT
Y499 FGTNQEDYASYIMNG
K529 ELLVQQTKNSDRTPL
K549 EGPPHSGKTALAAkI
K555-u GKTALAAkIAEESNF
K566 ESNFPFIKICsPDKM
S569-p FPFIKICsPDKMIGF
K572 IKICsPDKMIGFSET
K581 IGFSETAKCQAMKKI
K581 IGFSETAKCQAMKKI
K594 KIFDDAYKSQLSCVV
Y611 DIERLLDYVPIGPRF
K699 TTIAQQVKGKkVWIG
K699-u TTIAQQVkGKkVWIG
K702-u AQQVkGKkVWIGIkk
K708-m1 KkVWIGIkkLLMLIE
K709-m1 kVWIGIkkLLMLIEM
S739-p LMREEGAsPLDFD__
  rat

 
K27-u NCAVVNEkDYQSGQH
Y45 RTSPNHKYIFTLRTH
Y83 QDIEVALYSFDKAKQ
T94 KAKQCIGTMTIEIDF
K105 EIDFLQKKNIDSNPY
S109 LQKKNIDSNPYDTDK
K161-u AMDPSILkGEPASGk
K168-u kGEPASGkRQKIEVG
K198 SSLNLIGKAKTKENR
S207 KTKENRQSIINPDWN
K217-u NPDWNFEkMGIGGLD
K225-u MGIGGLDkEFSDIFR
S228 GGLDkEFSDIFRRAF
K254-u QMGCKHVkGILLyGP
Y259-p HVkGILLyGPPGCGk
K266-u yGPPGCGkTLLARQI
K275-u LLARQIGkMLNAREP
K283-u MLNAREPkVVNGPEI
K293-u NGPEILNkyVGESEA
Y294-p GPEILNkyVGESEAN
S298 LNkyVGESEANIRKL
K304 ESEANIRKLFADAEE
K335-u DEIDAICkQRGSMAG
S339 AICkQRGSMAGSTGV
S343 QRGSMAGSTGVHDTV
T349 GSTGVHDTVVNQLLS
S356 TVVNQLLSKIDGVEQ
K392-u RPGRLEVkMEIGLPD
K401-u EIGLPDEkGRLQILH
S421 MRGHQLLSADVDIkE
K427-u LSADVDIkELAVETk
K434-a kELAVETkNFSGAEL
K434-u kELAVETkNFSGAEL
S437 AVETkNFSGAELEGL
S450 GLVRAAQSTAMNRHI
S460 MNRHIKASTkVEVDM
T461 NRHIKASTkVEVDME
K462-u RHIKASTkVEVDMEk
K469-u kVEVDMEkAESLQVT
Y499-p FGTNQEDyASYIMNG
K529-u ELLVQQTkNSDRTPL
K549-u EGPPHSGkTALAAkI
K555-u GkTALAAkIAEESNF
K566-u ESNFPFIkICsPDkM
S569-p FPFIkICsPDkMIGF
K572-u IkICsPDkMIGFSET
K581-a IGFSETAkCQAMKKI
K581-u IGFSETAkCQAMKKI
K594-u KIFDDAYkSQLSCVV
Y611-p DIERLLDyVPIGPRF
K699-a TTIAQQVkGKKVWIG
K699-u TTIAQQVkGKKVWIG
K702 AQQVkGKKVWIGIKK
K708 KKVWIGIKKLLMLIE
K709 KVWIGIKKLLMLIEM
S739 LMREEGASPLDFD__
  hamster

 
K27 NCAVVSEKDYQSGQH
Y45 RTSPNHKYIFTLRTH
Y83 QEIEVALYSFDKAKQ
T94 KAKQCIGTMTIEIDF
K105 EIDFLQKKNIDSNPY
S109 LQKKNIDSNPYDTDK
K161 AMDPSILKGEPASGK
K168 KGEPASGKRQKIEVG
K198 SSLNLIGKAKTKENR
S207 KTKENRQSIINPDWN
K217 NPDWNFEKMGIGGLD
K225 MGIGGLDKEFSDIFR
S228 GGLDKEFSDIFRRAF
K254 QMGCKHVKGILLYGP
Y259 HVKGILLYGPPGCGK
K266 YGPPGCGKTLLARQI
K275 LLARQIGKMLNAREP
K283 MLNAREPKVVNGPEI
K293 NGPEILNKYVGESEA
Y294 GPEILNKYVGESEAN
S298 LNKYVGESEANIRKL
K304 ESEANIRKLFADAEE
K335 DEIDAICKQRGSMAG
S339 AICKQRGSMAGSTGV
S343 QRGSMAGSTGVHDTV
T349 GSTGVHDTVVNQLLS
S356 TVVNQLLSKIDGVEQ
K392 RPGRLEVKMEIGLPD
K401 EIGLPDEKGRLQILH
S421 MRGHQLLSADVDIKE
K427 LSADVDIKELAVETK
K434 KELAVETKNFSGAEL
K434 KELAVETKNFSGAEL
S437 AVETKNFSGAELEGL
S450 GLVRAAQSTAMNRHI
S460-p MNRHIKAstKVEVDM
T461-p NRHIKAstKVEVDME
K462 RHIKAstKVEVDMEK
K469 KVEVDMEKAESLQVT
Y499 FGTNQEDYASYIMNG
K529 ELLVQQTKNSDRTPL
K549 EGPPHSGKTALAAKI
K555 GKTALAAKIAEESNF
K566 ESNFPFIKICSPDKM
S569 FPFIKICSPDKMIGF
K572 IKICSPDKMIGFSET
K581 IGFSETAKCQAMKKI
K581 IGFSETAKCQAMKKI
K594 KIFDDAYKSQLSCVV
Y611 DIERLLDYVPIGPRF
K699 TTIAQQVKGKKVWIG
K699 TTIAQQVKGKKVWIG
K702 AQQVKGKKVWIGIKK
K708 KKVWIGIKKLLMLIE
K709 KVWIGIKKLLMLIEM
S739 LLREEGASPLDFD__
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.