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Protein Page:
JAK2 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
JAK2 a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; JakA family
Cellular Component: nuclear matrix; cytoskeleton; cytoplasm; caveola; cytosol; nucleus; lipid raft
Molecular Function: protein C-terminus binding; histone binding; acetylcholine receptor binding; non-membrane spanning protein tyrosine kinase activity; protein kinase binding; interleukin-12 receptor binding; protein kinase activity; protein binding; growth hormone receptor binding; peptide hormone receptor binding; protein-tyrosine kinase activity; insulin receptor substrate binding; heme binding; phosphoinositide 3-kinase binding; SH2 domain binding; type 1 angiotensin receptor binding; ATP binding; receptor binding
Biological Process: positive regulation of nitric oxide biosynthetic process; activation of MAPKK activity; positive regulation of apoptosis; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; tyrosine phosphorylation of JAK2 protein; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; response to antibiotic; elevation of cytosolic calcium ion concentration; tumor necrosis factor-mediated signaling pathway; erythrocyte differentiation; mesoderm development; negative regulation of neuron apoptosis; positive regulation of cell activation; positive regulation of DNA binding; positive regulation of protein import into nucleus, translocation; axon regeneration; positive regulation of insulin secretion; positive regulation of tumor necrosis factor production; JAK-STAT cascade; tyrosine phosphorylation of Stat5 protein; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; positive regulation of peptidyl-tyrosine phosphorylation; response to hydroperoxide; mineralocorticoid receptor signaling pathway; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription factor activity; positive regulation of cell differentiation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; hormone-mediated signaling; negative regulation of heart contraction; apoptosis; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; signal transduction; host programmed cell death induced by symbiont; negative regulation of cell proliferation; actin filament polymerization; positive regulation of cell proliferation; cell differentiation; STAT protein nuclear translocation; caspase activation; cytokine and chemokine mediated signaling pathway; negative regulation of DNA binding; G-protein coupled receptor protein signaling pathway; positive regulation of interleukin-1 beta production; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of inflammatory response; negative regulation of cell-cell adhesion; cell motility; blood coagulation; induction of apoptosis by oxidative stress; positive regulation of cell migration; positive regulation of inflammatory response
Reference #:  O60674 (UniProtKB)
Alt. Names/Synonyms: JAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2
Gene Symbols: JAK2
Molecular weight: 130,674 Da
Basal Isoelectric point: 6.82  Predict pI for various phosphorylation states
CST Pathways:  ErbB/HER Signaling  |  IL6 Signaling  |  Inhibition of Apoptosis  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

JAK2

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
enzymatic activity, inhibited: Y119‑p
molecular association, regulation: Y813‑p, Y1007‑p
phosphorylation: Y813‑p
protein stabilization: Y119‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
2 0 Y119-p VLYRIRFyFPRWYCS
1 1 H134 GSNRAYRHGISRGAE
1 0 K167-s DFVHGWIkVPVTHET
3 1 Y201-p DQTPLAIyNSISYKT
2 1 Y206 AIyNSISYKTFLPKC
9 6 Y221-p IRAKIQDyHILTRKR
0 1 K244 IQQFSQCKATARNLK
1 1 Y317 TEQDLQLYCDFPNII
2 1 Y372 FVSLIDGYYRLTADA
1 0 Y373 VSLIDGYYRLTADAH
0 1 K415 FAISKLKKAGNQTGL
1 3 Y423-p AGNQTGLyVLRCSPK
1 4 Y435-p SPKDFNKyFLTFAVE
0 1 K468 EYNLSGTKKNFSSLK
4 11 S523-p GVSDVPTsPTLQRPT
8 828 Y570-p VRREVGDyGQLHETE
1 0 Y613 HKHLVLNYGVCVCGD
1 0 K630-s ILVQEFVkFGSLDTy
3 2 Y637-p kFGSLDTyLKKNKNC
1 1 T668 MHFLEENTLIHGNVC
0 2 Y790-p LINNCMDyEPDFRPS
7 4 Y813-p NSLFTPDyELLTEND
3 2 Y868 GSVEMCRYDPLQDNT
1 6 S904-p REIEILKsLQHDNIV
1 0 K912-s LQHDNIVkYkGVCYS
1 0 Y913 QHDNIVkYkGVCYSA
1 0 K914-s HDNIVkYkGVCYSAG
1 0 Y918 VkYkGVCYSAGRRNL
1 6 Y931-p NLKLIMEyLPYGSLR
1 0 Y934 LIMEyLPYGSLRDyL
1 1 Y940-p PYGSLRDyLQKHKER
0 3 Y956-p DHIKLLQyTSQICKG
3 6 Y966-p QICKGMEyLGTKRyI
3 1 Y972-p EyLGTKRyIHRDLAT
1 0 K991-s VENENRVkIGDFGLT
49 19 Y1007-p VLPQDKEyyKVkEPG
45 17 Y1008-p LPQDKEyyKVkEPGE
1 0 K1011-s DKEyyKVkEPGESPI
3774 : Phospho-Jak2 (Tyr221) Antibody
3771 : Phospho-Jak2 (Tyr1007/1008) Antibody
3776 : Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
4406 : Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb
3771 : Phospho-Jak2 (Tyr1007/1008) Antibody
3776 : Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
8082 : Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb
8149 : Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb (PE Conjugate)
  mouse

 
Y119-p ILYRIRFyFPHWYCS
Y134-p GSSRTYRyGVSRGAE
K167 DFVHGWIKVPVTHET
Y201-p DQTPLAVyNSVSyKT
Y206-p AVyNSVSyKTFLPKC
Y221-p VRAKIQDyHILTRKR
K244-u IQQFSQCkATARNLK
Y317-p TEQDVQLyCDFPDII
Y372-p FVSLIDGyyRLTADA
Y373-p VSLIDGyyRLTADAH
K415-u FAISKLKkAGNQTGL
Y423-p AGNQTGLyVLRCSPK
Y435-p SPKDFNKyFLTFAVE
K468-u EYNLSGTkRNFSNLK
S523-p GISDVQIsPTLQRHN
Y570-p VRREVGDyGQLHKTE
Y613-p HKHLVLNyGVCVCGE
K630 ILVQEFVKFGSLDTy
Y637-p KFGSLDTyLKKNKNS
S668-p MHFLEEKsLIHGNVC
Y790 LINNCMDYEPDFRPA
Y813-p NSLFTPDyELLTEND
Y868-p GSVEMCRyDPLQDNT
S904-p REIEILKsLQHDNIV
K912 LQHDNIVKyKGVCyS
Y913-p QHDNIVKyKGVCySA
K914 HDNIVKyKGVCySAG
Y918-p VKyKGVCySAGRRNL
Y931-p NLRLIMEyLPyGSLR
Y934-p LIMEyLPyGSLRDyL
Y940-p PyGSLRDyLQKHKER
Y956 DHKKLLQYTSQICKG
Y966-p QICKGMEyLGTKRyI
Y972-p EyLGTKRyIHRDLAT
K991 VENENRVKIGDFGLT
Y1007-p VLPQDKEyyKVKEPG
Y1008-p LPQDKEyyKVKEPGE
K1011 DKEyyKVKEPGESPI
3774 : Phospho-Jak2 (Tyr221) Antibody
3771 : Phospho-Jak2 (Tyr1007/1008) Antibody
3776 : Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
4406 : Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb
3771 : Phospho-Jak2 (Tyr1007/1008) Antibody
3776 : Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
8082 : Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb
8149 : Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb (PE Conjugate)
  rat

 
Y119 ILYRIRFYFPHWYCS
Y134 GSNRTYRYGVSRGAE
K167 DFVHGWIKVPVTHET
Y201 DQTPLAVYNSISYKT
Y206 AVYNSISYKTFLPKC
Y221 VRAKIQDYHILTRKR
K244 IQQFSQCKATARNLK
Y317 TEQDLQLYCDFPDII
Y372 FVSLIDGYYRLTADA
Y373 VSLIDGYYRLTADAH
K415 FAISKLKKAGNQTGL
Y423 AGNQTGLYVLRCSPK
Y435 SPKDFNKYFLTFAVE
K468 EYNLSGTKRNFSSLK
S523 GVSDVQLSPTLQRHN
Y570-p VRREVGDyGQLHETE
Y613 HKHLVLNYGVCVCGE
K630 ILVQEFVKFGSLDTY
Y637 KFGSLDTYLKKNKNS
S668 MHFLEEKSLIHGNVC
Y790 LINTCMDYEPDFRPA
Y813 NSLFTPDYELLTEND
Y868 GSVEMCRYDPLQDNT
S904 REIEILKSLQHDNIV
K912 LQHDNIVKYKGVCYS
Y913 QHDNIVKYKGVCYSA
K914 HDNIVKYKGVCYSAG
Y918 VKYKGVCYSAGRRNL
Y931 NLRLIMEYLPYGSLR
Y934 LIMEYLPYGSLRDYL
Y940 PYGSLRDYLQKHKER
Y956 DHKKLLQYTSQICKG
Y966 QICKGMEYLGTKRYI
Y972 EYLGTKRYIHRDLAT
K991 VENENRVKIGDFGLT
Y1007-p VLPQDKEyyKVKEPG
Y1008-p LPQDKEyyKVKEPGE
K1011 DKEyyKVKEPGESPI
3774 : Phospho-Jak2 (Tyr221) Antibody
3771 : Phospho-Jak2 (Tyr1007/1008) Antibody
3776 : Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
4406 : Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb
3771 : Phospho-Jak2 (Tyr1007/1008) Antibody
3776 : Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
8082 : Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb
8149 : Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb (PE Conjugate)
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