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Protein Page:
IRE1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
IRE1 a ser/thr protein kinase that possess endonuclease activity. Important in altering gene expression as a response to endoplasmic reticulum based stress signals. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain, which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ubiquitously expressed. High levels observed in pancreatic tissue. Functionally connected with insulin biosynthesis in pancreatic beta cells. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of IRE1. Note: This description may include information from UniProtKB.
Protein type: Apoptosis; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Membrane protein, integral; Ribonuclease; Kinase, protein; EC 2.7.11.1; Endoplasmic reticulum; Chaperone; Other group; IRE family
Chromosomal Location of Human Ortholog: 17q24.2
Cellular Component: endoplasmic reticulum membrane; mitochondrion; integral to endoplasmic reticulum membrane; nuclear inner membrane
Molecular Function: endoribonuclease activity; identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; magnesium ion binding; ATP binding
Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; transcription, DNA-dependent; unfolded protein response; protein amino acid autophosphorylation; cell cycle arrest; protein amino acid phosphorylation
Reference #:  O75460 (UniProtKB)
Alt. Names/Synonyms: endoplasmic reticulum to nucleus signaling 1; endoplasmic reticulum to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; Inositol-requiring protein 1; IRE1; Ire1-alpha; IRE1a; IRE1P; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase; Serine/threonine-protein kinase/endoribonuclease IRE1
Gene Symbols: ERN1
Molecular weight: 109,735 Da
Basal Isoelectric point: 5.98  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

IRE1

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: S724‑p
enzymatic activity, induced: S724‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T49-p LHAVSKRtGSIKWTL
0 1 T132-p WYVIDLLtGEKQQTL
0 1 Y161-p LYLGRTEyTITMyDT
0 2 Y166-p TEyTITMyDTKTREL
0 1 Y179-p ELRWNATyFDYAASL
0 1 T283-p AKSKLTPtLYVGKyS
0 1 Y289-p PtLYVGKySTSLYAS
0 1 K374-ac TPLSASTkMLERFPN
0 1 S544-p LCSGSSAsKAGsSPs
0 1 S548-p SSAsKAGsSPsLEQD
0 2 S551-p sKAGsSPsLEQDDGD
0 1 K633-ub IRYFCTEkDRQFQYI
0 1 K706 PNAHGKIKAMISDFG
4 0 S724-p KLAVGRHsFsRRSGV
0 1 S726-p AVGRHsFsRRSGVPG
0 1 K811 IARELIEKMIAMDPQ
0 1 K871 RGGRAVVKMDWRENI
0 5 T973-p PEPQPPVtPDAL___
  mouse

 
T51 LHAVSKRTGSIKWTL
T134 WYVIDLLTGEKQQTL
Y163 LYLGRTEYTITMYDT
Y168 TEYTITMYDTKTREL
Y181 ELRWNATYFDYAASL
T285 AKSKLTPTLYVGKYS
Y291 PTLYVGKYSTSLYAS
K376 TPLSASTKMLERFPN
S544 LHPSSSASRAGTSPs
T548 SSASRAGTSPsLEQD
S551-p SRAGTSPsLEQDDED
K633 IRYFCTEKDRQFQYI
K706-ub PNAHGRIkAMISDFG
S724-p KLAVGRHsFSRRSGV
S726 AVGRHsFSRRSGVPG
K811-ub IARELIEkMIAMDPQ
K871-ub RGGRAVVkMDWRENI
I973 TEPQPPVIPYAL___
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