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Protein Page:
DNMT1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
DNMT1 an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described. Note: This description may include information from UniProtKB.
Protein type: Methyltransferase, DNA; Transcription regulation; EC 2.1.1.37; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; Methyltransferase
Cellular Component: centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; identical protein binding; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; transcription factor binding; DNA-methyltransferase activity
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; DNA methylation; gene silencing; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin modification; regulation of cell proliferation
Reference #:  P26358 (UniProtKB)
Alt. Names/Synonyms: AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5-)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA MTase HsaI; DNMT; DNMT1; FLJ16293; M.HsaI; MCMT; MGC104992
Gene Symbols: DNMT1
Molecular weight: 183,165 Da
Basal Isoelectric point: 7.99  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

DNMT1
Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: S154‑p
methylation: S143‑p
phosphorylation: K142‑m1
protein degradation: K142‑m1
protein stabilization: S143‑p, S154‑p
ubiquitination: K142‑m1

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 6 V15 ARVPTLAVPAISLPD
0 1 S19 TLAVPAISLPDDVRR
0 1 K70-m2 DLETKLRkEELSEEG
0 1 S84-p GYLAKVKsLLNkDLS
0 1 K88-u KVKsLLNkDLSLENG
0 11 S127-p VGMADANsPPKPLsk
0 2 P128 GMADANsPPKPLskP
0 1 P131 DANsPPKPLskPRtP
0 6 S133-p NsPPKPLskPRtPRR
0 1 K134-u sPPKPLskPRtPRRs
0 2 T137-p KPLskPRtPRRsksD
0 8 S141-p kPRtPRRsksDGEAk
1 0 K142 PRtPRRsKsDGEAkP
1 0 K142-m1 PRtPRRsksDGEAkP
1 14 S143-p RtPRRsksDGEAkPE
0 1 A147 RsksDGEAkPEPsPs
1 0 K148-s sksDGEAkPEPsPsP
1 1 - gap
0 3 - gap
0 1 - gap
0 7 S152-p GEAkPEPsPsPRITR
1 10 S154-p AkPEPsPsPRITRkS
0 1 P155 kPEPsPsPRITRkST
1 0 K160-a PsPRITRkSTRQttI
0 1 T165-p TRkSTRQttITSHFA
0 1 T166-p RkSTRQttITSHFAk
0 1 K173-a tITSHFAkGPAKRKP
0 1 S184 KRKPQEESERAksDE
1 0 K188-a QEESERAksDESIkE
0 2 S189-p EESERAksDESIkEE
0 1 S192 ERAksDESIkEEDKD
1 0 K194-s AksDESIkEEDKDQD
0 3 T208-p DEKRRRVtsRERVAR
0 10 S209-p EKRRRVtsRERVARP
0 1 S227-p EEPERAKsGTRTEKE
0 1 - gap
0 11 T249 EKRLRSQTKEPTPKQ
0 1 T253 RSQTKEPTPKQKLkE
0 4 L258 EPTPKQKLkEEPDRE
1 0 K259-a PTPKQKLkEEPDREA
0 3 S288 KDEKKHRSQPKDLAA
0 6 I311 PEKVNPQIsDEKDED
0 1 S312-p EKVNPQIsDEKDEDE
0 1 K336-u PKEPTEKkMARAKTV
0 1 S346-p RAKTVMNsKTHPPKC
0 2 Y359-p KCIQCGQyLDDPDLk
1 0 K366-a yLDDPDLkYGQHPPD
0 1 K385-u PQMLTNEkLSIFDAN
0 1 A391 EkLSIFDANEsGFEs
0 10 S394-p SIFDANEsGFEsyEA
0 5 S398-p ANEsGFEsyEALPQH
0 28 Y399-p NEsGFEsyEALPQHK
0 1 S492 EYILMDPSPEYAPIF
2 2 S509 MQEKIYISKIVVEFL
0 8 K586-u PCMRDLIkLAGVtLG
0 3 T591-p LIkLAGVtLGQRRAQ
0 1 T608 RQTIRHSTREKDRGP
0 1 R609 QTIRHSTREKDRGPT
0 2 K622 PTKATTTKLVYQIFD
0 15 K666-u CQQPECGkCkACKDM
0 5 K668-u QPECGkCkACKDMVk
0 3 K671 CGkCkACKDMVkFGG
0 4 K675-u kACKDMVkFGGSGRS
1 0 K675-s kACKDMVkFGGSGRS
0 1 P710 EEVDDNIPEMPsPKK
0 39 S714-p DNIPEMPsPKKMHQG
0 20 S732-p KQNKNRIsWVGEAVk
1 0 K739-s sWVGEAVkTDGKKSY
1 0 K749-a GKKSYYKkVCIDAET
0 1 T882-p RFESPPKtQPTEDNK
1 0 K891-a PTEDNKFkFCVSCAR
0 1 K928-u VLYYSATkNGILYRV
0 3 S954-p TFNIKLSsPVkRPRk
1 0 K957-a IKLSsPVkRPRkEPV
1 0 K957-s IKLSsPVkRPRkEPV
1 0 K961-a sPVkRPRkEPVDEDL
0 1 K961-u sPVkRPRkEPVDEDL
0 50 Y969-p EPVDEDLyPEHYRkY
1 0 K975-a LyPEHYRkYSDYIkG
0 3 K981-u RkYSDYIkGSNLDAP
0 1 K997-u PYRIGRIkEIFCPKK
1 0 K1054-a EEAVVDFkAVQGRCT
0 1 K1092-u FLEAYNAkSKSFEDP
0 4 S1105-p DPPNHARsPGNKGkG
1 2 K1111-a RsPGNKGkGkGkGkG
1 5 K1113-a PGNKGkGkGkGkGkP
1 13 K1115-a NKGkGkGkGkGkPkS
2 20 K1117-a GkGkGkGkGkPkSQA
1 18 K1119-a GkGkGkGkPkSQACE
1 18 K1121-a GkGkGkPkSQACEPS
1 0 K1135-s SEPEIEIkLPKLRTL
0 1 K1196-u EDCNILLkLVMAGET
0 1 T1204-p LVMAGETtNSRGQRL
0 1 S1246 TYSKFKNSLVVSFLS
0 1 S1250 FKNSLVVSFLSyCDY
0 1 Y1254-p LVVSFLSyCDYYRPR
0 6 K1323-u LAAAPGEkLPLFPEP
1 0 K1349-a SVVVDDKkFVSNITR
0 5 Y1405-p RQLRGAQyQPILRDH
1 0 K1415-a ILRDHICkDMSALVA
0 1 S1418 DHICkDMSALVAARM
0 1 N1493 DPAARQFNTLIPWCL
0 1 K1535-u TNPEPMGkQGRVLHP
0 1 K1572-u LFGNILDkHRQVGNA
0 1 K1586-u AVPPPLAkAIGLEIK
0 1 S1605-p AKARESAsAKIkEEE
1 0 K1609-s ESAsAKIkEEEAAKD
  DNMT1 iso2  
V15 ARVPTLAVPAISLPD
S19 TLAVPAISLPDDVRR
K70 DLETKLRKEELSEEG
S84 GYLAKVKSLLNKDLS
K88 KVKSLLNKDLSLENG
S127 VGMADANSPPKPLSK
P128 GMADANSPPKPLSKP
P131 DANSPPKPLSKPRTP
S133 NSPPKPLSKPRTPRR
K134 SPPKPLSKPRTPRRS
T137 KPLSKPRTPRRSKSD
S141 KPRTPRRSKSDGEAK
K142 PRTPRRSKSDGEAKR
K142 PRTPRRSKSDGEAKR
S143 RTPRRSKSDGEAKRS
A147 RSKSDGEAKRSRDPP
K148 SKSDGEAKRSRDPPA
- gap
S158-p RDPPASAsQVtGIRA
T161-p PASAsQVtGIRAEPS
S168 tGIRAEPSPSPRITR
S170 IRAEPSPSPRITRKS
P171 RAEPSPSPRITRKST
K176 PSPRITRKSTRQTTI
T181 TRKSTRQTTITSHFA
T182 RKSTRQTTITSHFAK
K189 TITSHFAKGPAKRKP
S200 KRKPQEESERAKSDE
K204 QEESERAKSDESIKE
S205 EESERAKSDESIKEE
S208 ERAKSDESIKEEDKD
K210 AKSDESIKEEDKDQD
T224 DEKRRRVTSRERVAR
S225 EKRRRVTSRERVARP
S243 EEPERAKSGTRTEKE
- gap
T265 EKRLRSQTKEPTPKQ
T269 RSQTKEPTPKQKLKE
L274 EPTPKQKLKEEPDRE
K275 PTPKQKLKEEPDREA
S304 KDEKKHRSQPKDLAA
I327 PEKVNPQISDEKDED
S328 EKVNPQISDEKDEDE
K352 PKEPTEKKMARAKTV
S362 RAKTVMNSKTHPPKC
Y375 KCIQCGQYLDDPDLK
K382 YLDDPDLKYGQHPPD
K401 PQMLTNEKLSIFDAN
A407 EKLSIFDANESGFES
S410 SIFDANESGFESYEA
S414 ANESGFESYEALPQH
Y415 NESGFESYEALPQHK
S508 EYILMDPSPEYAPIF
S525 MQEKIYISKIVVEFL
K602 PCMRDLIKLAGVTLG
T607 LIKLAGVTLGQRRAQ
T624 RQTIRHSTREKDRGP
R625 QTIRHSTREKDRGPT
K638 PTKATTTKLVYQIFD
K682 CQQPECGKCKACKDM
K684 QPECGKCKACKDMVK
K687 CGKCKACKDMVKFGG
K691 KACKDMVKFGGSGRS
K691 KACKDMVKFGGSGRS
P726 EEVDDNIPEMPSPKK
S730 DNIPEMPSPKKMHQG
S748 KQNKNRISWVGEAVK
K755 SWVGEAVKTDGKKSY
K765 GKKSYYKKVCIDAET
T898 RFESPPKTQPTEDNK
K907 PTEDNKFKFCVSCAR
K944 VLYYSATKNGILYRV
S970 TFNIKLSSPVKRPRK
K973 IKLSSPVKRPRKEPV
K973 IKLSSPVKRPRKEPV
K977 SPVKRPRKEPVDEDL
K977 SPVKRPRKEPVDEDL
Y985 EPVDEDLYPEHYRKY
K991 LYPEHYRKYSDYIKG
K997 RKYSDYIKGSNLDAP
K1013 PYRIGRIKEIFCPKK
K1070 EEAVVDFKAVQGRCT
K1108 FLEAYNAKSKSFEDP
S1121 DPPNHARSPGNKGKG
K1127 RSPGNKGKGKGKGKG
K1129 PGNKGKGKGKGKGKP
K1131 NKGKGKGKGKGKPKS
K1133 GKGKGKGKGKPKSQA
K1135 GKGKGKGKPKSQACE
K1137 GKGKGKPKSQACEPS
K1151 SEPEIEIKLPKLRTL
K1212 EDCNILLKLVMAGET
T1220 LVMAGETTNSRGQRL
S1262 TYSKFKNSLVVSFLS
S1266 FKNSLVVSFLSYCDY
Y1270 LVVSFLSYCDYYRPR
K1339 LAAAPGEKLPLFPEP
K1365 SVVVDDKKFVSNITR
Y1421 RQLRGAQYQPILRDH
K1431 ILRDHICKDMSALVA
S1434 DHICKDMSALVAARM
N1509 DPAARQFNTLIPWCL
K1551 TNPEPMGKQGRVLHP
K1588 LFGNILDKHRQVGNA
K1602 AVPPPLAKAIGLEIK
S1621 AKARESASAKIKEEE
K1625 ESASAKIKEEEAAKD
  mouse

 
S15-p ARVPALAsPAGsLPD
S19-p ALAsPAGsLPDHVRR
K70 DLETKLHKEELSEEG
S84-p GYLAKVKsLLNKDLS
K88 KVKsLLNKDLSLENG
R124 AEMADSNRsPRsRPK
S125-p EMADSNRsPRsRPKP
S128-p DSNRsPRsRPKPRGP
P130 NRsPRsRPKPRGPRR
K131 RsPRsRPKPRGPRRs
G134 RsRPKPRGPRRsksD
S138-p KPRGPRRsksDSDtL
K139-m PRGPRRsksDSDtLs
K139 PRGPRRsKsDSDtLs
S140-p RGPRRsksDSDtLsV
T144-p RsksDSDtLsVETsP
- gap
S146-p ksDSDtLsVETsPss
- gap
- gap
S150-p DtLsVETsPssVATR
S152-p LsVETsPssVATRRT
S153-p sVETsPssVATRRTT
R158 PssVATRRTTRQTTI
T163 TRRTTRQTTITAHFT
T164 RRTTRQTTITAHFTK
K171 TITAHFTKGPTKRKP
S182-p KRKPKEEsEEGNsAE
N186 KEEsEEGNsAEsAAE
S187-p EEsEEGNsAEsAAEE
S190-p EEGNsAEsAAEERDQ
A192 GNsAEsAAEERDQDK
T206 KKRRVVDTESGAAAA
- gap
A222 EKLEEVTAGTQLGPE
S240-p EQEDDNRsLRRHtRE
T245-p NRsLRRHtRELsLRR
S249-p RRHtRELsLRRKsKE
S254-p ELsLRRKsKEDPDRE
K255 LsLRRKsKEDPDREA
S285-p KRSSRPRsQPRDPAA
T308-p PEQVAPEtPEDRDED
- gap
K343 VQSRSERKAAQSKSV
- gap
H365 KCPECGQHLDDPNLK
K372 HLDDPNLKYQQHPED
K391 PQMLTSEKLSIYDsT
S397-p EKLSIYDsTSTWFDT
T400 SIYDsTSTWFDTYED
T404 sTSTWFDTYEDSPMH
Y405 TSTWFDTYEDSPMHR
S498-p EYILMEPsKEYEPIF
S515-p MQEKIYIsKIVVEFL
H592 PCMRALIHLAGVsLG
S597-p LIHLAGVsLGQRRAT
T611-p TRRVMGAtkEKDKAP
K612-u RRVMGAtkEKDKAPT
K625-u PTKATTTkLVYQIFD
K669-u CQQPECGkCkACkDM
K671-u QPECGkCkACkDMVk
K674-u CGkCkACkDMVkFGG
K678-u kACkDMVkFGGTGRS
K678 kACkDMVKFGGTGRS
S713-p EEADDDVsEMPsPKK
S717-p DDVsEMPsPKKLHQG
S735 KQNKDRISWLGQPMK
K742 SWLGQPMKIEENRTY
K752 ENRTYYQKVSIDEEM
T886 RFESPPKTQPTEDNK
K895 PTEDNKHKFCLSCIR
K932 VYCSSITKNGVVYRL
S958-p TFNIKVAsPVKRPKK
K961 IKVAsPVKRPKKDPV
K961 IKVAsPVKRPKKDPV
K965 sPVKRPKKDPVNETL
K965 sPVKRPKKDPVNETL
Y973 DPVNETLYPEHYRKY
K979 LYPEHYRKYSDYIkG
K985-u RKYSDYIkGSNLDAP
K1001 PYRIGRIKEIHCGKK
S1057 EEAVVNFSDVQGRCT
K1095 FLEAYNSKTKNFEDP
S1108-p DPPNHARsPGNKGKG
K1114 RsPGNKGKGKGKGKG
K1116 PGNKGKGKGKGKGKG
K1118 NKGKGKGKGKGKGKH
K1120 GKGKGKGKGKGKHQV
K1122 GKGKGKGKGKHQVSE
K1124 GKGKGKGKHQVSEPK
K1138 KEPEAAIKLPKLRTL
K1199 EDCNVLLKLVMAGEV
T1207 LVMAGEVTNSLGQRL
S1249-p TYSKFKNsLVVsFLS
S1253-p FKNsLVVsFLSYCDY
Y1257 LVVsFLSYCDYYRPR
K1326-u LAAAPGEkLPLFPEP
K1352 SVVVDDKKFVSNITR
Y1408 RQLRGSHYQPILRDH
K1418 ILRDHICKDMsPLVA
S1421-p DHICKDMsPLVAARM
S1495-p DPESRQFsTLIPWCL
K1537 TNPEPMGKQGRVLHP
R1574 FFGNILDRHRQVGNA
K1588 AVPPPLAKAIGLEIK
S1607 SSARESASAAVKAKE
K1611 ESASAAVKAKEEAAT
  rat

 
S15 ARVPALASPAGSLPD
S19 ALASPAGSLPDHVRR
K70 DLETKLHKEELSEEG
T84 GYLAKVKTLLNKDLC
K88 KVKTLLNKDLCLENG
R124 AEMADSNRSPRSRPK
S125 EMADSNRSPRSRPKP
S128 DSNRSPRSRPKPRGP
P130 NRSPRSRPKPRGPRR
K131 RSPRSRPKPRGPRRS
G134 RSRPKPRGPRRSKSD
S138 KPRGPRRSKSDSETM
K139 PRGPRRSKSDSETMI
K139 PRGPRRSKSDSETMI
S140 RGPRRSKSDSETMIE
T144 RSKSDSETMIEASSS
- gap
- gap
- gap
- gap
S149 SETMIEASSSSVATR
S151 TMIEASSSSVATRRT
S152 MIEASSSSVATRRTT
R157 SSSVATRRTTRQTTI
T162 TRRTTRQTTITSHFK
T163 RRTTRQTTITSHFKG
K169 TTITSHFKGPAKRKP
S180 KRKPKEDSEKGNANE
N184 KEDSEKGNANESAAE
A185 EDSEKGNANESAAEE
S188 EKGNANESAAEERDQ
A190 GNANESAAEERDQDK
T204 KKRRVAGTESRASRA
- gap
P223 EKPERVRPGTQLCQE
- gap
T245 DRRPRRQTRELASRR
A249 RRQTRELASRRKsRE
S254-p ELASRRKsREDPDRE
R255 LASRRKsREDPDREA
S285 KRSSRPRSQPRDLAT
P307 VEQITPEPPEGKDED
- gap
K342 VQSRVERKASQGKAS
- gap
Y365 QCPECGQYLDDPDLK
K372 YLDDPDLKYQQHPVD
A391 PQMLTNEALSVFDSN
S397 EALSVFDSNSSWFET
S400 SVFDSNSSWFETYDS
T404 SNSSWFETYDSSPMH
Y405 NSSWFETYDSSPMHK
S498 EYFLMEPSPEYAPIF
S515 MQEKIYISKIVVEFL
H592 PCMRSLIHLAGVSLG
S597 LIHLAGVSLGQRRAT
A612 RRTVINSAKVKRKGP
K613 RTVINSAKVKRKGPT
K626 PTKATTTKLVYQIFD
K670 CQQPECGKCKACKDM
K672 QPECGKCKACKDMVK
K675 CGKCKACKDMVKFGG
K679 KACKDMVKFGGTGRS
K679 KACKDMVKFGGTGRS
P714 EEADDDIPELPSPKK
S718 DDIPELPSPKKLHQG
S736 KQNKDRISWLGEPVK
K743 SWLGEPVKIEENRTY
K753 ENRTYYWKVSIDEET
T886 RFESPPKTQPAEDNK
K895 PAEDNKHKFCLSCIR
K932 VYCSSITKNGVVYRL
S958 TFNIKMASPMKRSKR
K961 IKMASPMKRSKRDPV
K961 IKMASPMKRSKRDPV
R965 SPMKRSKRDPVNENP
R965 SPMKRSKRDPVNENP
V973 DPVNENPVPRDTYRK
K980 VPRDTYRKYSDYIKG
K986 RKYSDYIKGSNLDAP
K1002 PYRIGRIKEIYCGKK
S1059 EEAVVDFSDVQGRCT
K1097 FLEAYNSKTKSFEDP
S1110 DPPNHARSPGNKGKG
K1116 RSPGNKGKGKGKGKG
K1118 PGNKGKGKGKGKGKG
K1120 NKGKGKGKGKGKGKP
K1122 GKGKGKGKGKGKPQV
K1124 GKGKGKGKGKPQVSE
K1126 GKGKGKGKPQVSEPK
K1140 KEPEAAIKLPKLRTL
K1201 EDCNVLLKLVMAGEV
T1209 LVMAGEVTNSLGQRL
S1251 TYSKFKNSLVVSFLS
S1255 FKNSLVVSFLSYCDY
Y1259 LVVSFLSYCDYYRPR
K1328 LAAAPGEKLPLFPEP
K1354 SVVVDDKKFVSNITR
L1410 EAAARVALPAHPQGP
Q1420 HPQGPYPQVHERAGG
E1423 GPYPQVHERAGGCRM
N1497 DPASRQFNTLIPWCL
K1539 TNPEPMGKQGRVLHP
R1576 LFGNILDRHRQVGNA
K1590 AVPPPLAKAIGLEIK
S1609 ASAQESASAAVKGKE
K1613 ESASAAVKGKEETTT
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