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Protein Page:
NR1D1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NR1D1 a widely expressed member of the orphan nuclear receptor family of proteins. Expressed at relatively high levels in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and its ectopic expression in 3T3-L1 cells potentiates adipocyte differentiation. A metabolic regulator whose expression is increased in HER2-positive breast cancer cells. Regulates malate dehydrogenase 1 (MDH1) and malic enzyme 1 (ME1), enzymes that link glycolysis and fatty acid synthesis. May contribute to the abnormal cellular energy metabolism observed in HER2-positive breast cancers. Its expression oscillates with circadian rhythm in liver cells, regulating the expression of BMAL1, ApoA1 and ApoC3, all key regulators of circadian rhythm. Represses the basal activity of the mouse Bmal1 gene promoter. Phosphorylation by GSK3b at S55/59 stabilizes NRD1 protein levels. Regulates inflammation by targeting the NF-??B responsive genes IL-6 and COX-2. NR1D1 lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor N-CoR1/HDAC3 complex to target genes to repress transcription. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; Nuclear receptor
Cellular Component: nucleoplasm; dendrite; cytoplasm; nuclear chromatin; dendritic spine; nucleus
Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; heme binding; steroid hormone receptor activity; transcription corepressor activity
Biological Process: proteasomal protein catabolic process; circadian thermoregulation; transcription initiation from RNA polymerase II promoter; glycogen biosynthetic process; intracellular receptor-mediated signaling pathway; regulation of lipid metabolic process; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; negative regulation of toll-like receptor 4 signaling pathway; steroid hormone mediated signaling; gene expression; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; cell differentiation
Reference #:  P20393 (UniProtKB)
Alt. Names/Synonyms: EAR-1; EAR1; HREV; NR1D1; Nuclear receptor subfamily 1 group D member 1; nuclear receptor subfamily 1, group D, member 1; Rev-erbA-alpha; Rev-ErbAalpha; THRA1; THRAL; thyroid hormone receptor, alpha-like; V-erbA-related protein 1
Gene Symbols: NR1D1
Molecular weight: 66,805 Da
Basal Isoelectric point: 8.83  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NR1D1

Protein Structure Not Found.


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Sites Implicated In
protein stabilization: S55‑p, S59‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S22 TYIGSSGSSPSRTSP
0 1 S25 GSSGSSPSRTSPESL
1 2 S55-p CPTYFPPsPTGsLTQ
1 0 S59-p FPPsPTGsLTQDPAR
0 1 S76 GSIPPSLSDDGSPSS
0 2 S86-p GSPSSSSsSSSSSSS
0 1 S88 PSSSSsSSSSSSSFY
0 1 S89 SSSSsSSSSSSSFYN
0 1 S93 sSSSSSSSFYNGSPP
0 1 S222 RMLAEMQSAMNLANN
0 1 S320-p NFNANHAsGSPPATT
0 1 S322 NANHAsGSPPATTPH
0 1 Y394-p RLCPTHVyAAPEGKA
0 1 K412 SPRQGNSKNVLLACP
0 2 K455-ub REVVEFAkHIPGFRD
0 1 S486-p VLMVRFAsLFNVKDQ
0 1 K576-ub ALRALVLkNRPLETS
0 2 K591-ub RFTKLLLkLPDLRTL
0 1 K605 LNNMHSEKLLSFRVD
2129 : Phospho-Rev-erbalpha (Ser55/59) Antibody
2129 : Phospho-Rev-erbalpha (Ser55/59) Antibody
  mouse

 
S22-p TYIGSSGsSPsRTSP
S25-p GSSGsSPsRTSPESL
S55-p CPTYFPPsPTGSLTQ
S59 FPPsPTGSLTQDPAR
S76-p GSAPPSLsDDSSPSS
S87-p SPSSASSsSssSSSs
S89-p SSASSsSssSSSsFY
S90-p SASSsSssSSSsFYN
S94-p sSssSSSsFYNGSPP
S223-p RMLAEMQsAMNLANN
S321 NFNANHASGsPSATT
S323-p NANHASGsPSATTPH
Y395 RLCPTHVYSAPEGEA
K413-ub SLRQGNTkNVLLACP
K456-ub REVVEFAkHIPGFRD
S487 VLMVRFASLFNVKDQ
K577 ALRALVLKNRPSETS
K592-ub RFTKLLLkLPDLRTL
K606-ub LNNMHSEkLLSFRVD
2129 : Phospho-Rev-erbalpha (Ser55/59) Antibody
2129 : Phospho-Rev-erbalpha (Ser55/59) Antibody
  rat

 
S22 TYIGSSGSSPSRTSP
S25 GSSGSSPSRTSPESL
S55-p CPTYFPPsPTGSLTQ
S59 FPPsPTGSLTQDPAR
S76 GTVPPSLSDDSSPSS
S87 SPSSASSSSSSSSSS
S89 SSASSSSSSSSSSFY
S90 SASSSSSSSSSSFYN
S94 SSSSSSSSFYNGSPP
N223 RMLAEMQNAMNLANN
S321 NFNANHASGSPPATT
S323 NANHASGSPPATTPQ
Y395 RLCPTHVYSAPEGKA
K413 GLRQGNTKNVLLACP
K456 REVVEFAKHIPGFRD
S487 VLMVRFASLFNVKDQ
K577 ALRALVLKNRPSETS
K592 RFTKLLLKLPDLRTL
K606 LNNMHSEKLLSFRVD
2129 : Phospho-Rev-erbalpha (Ser55/59) Antibody
2129 : Phospho-Rev-erbalpha (Ser55/59) Antibody
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