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Protein Page:
GSK3B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GSK3B a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation Note: This description may include information from UniProtKB.
Protein type: Protein kinase, CMGC; EC 2.7.11.26; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; GSK family; GSK subfamily
Chromosomal Location of Human Ortholog: 3q13.3
Cellular Component: centrosome; dendritic spine; ribonucleoprotein complex; cytosol; beta-catenin destruction complex; lipid raft; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus
Molecular Function: integrin binding; NF-kappaB binding; protein serine/threonine kinase activity; ionotropic glutamate receptor binding; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; tau protein binding; protein kinase binding; ATP binding
Biological Process: fat cell differentiation; circadian rhythm; glycogen metabolic process; negative regulation of MAP kinase activity; axon guidance; genetic imprinting; re-entry into mitotic cell cycle; nerve growth factor receptor signaling pathway; positive regulation of protein binding; response to lithium ion; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; response to drug; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; organ morphogenesis; peptidyl-serine phosphorylation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of protein catabolic process; epithelial to mesenchymal transition; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Rac GTPase activity; negative regulation of protein complex assembly; regulation of neuronal synaptic plasticity; negative regulation of apoptosis
Reference #:  P49841 (UniProtKB)
Alt. Names/Synonyms: glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta isoform
Gene Symbols: GSK3B
Molecular weight: 46,744 Da
Basal Isoelectric point: 8.98  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Alzheimer's Disease  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  G1/S Checkpoint  |  Growth And Differentiation Control by MAPKs  |  Hedgehog Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Microtubule Dynamics  |  mTOR Signaling  |  NF-kB Signaling  |  PI3K/Akt Signaling  |  Translation: eIF2  |  Translation: eIF4E and p70S6K  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GSK3B

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S9‑p, Y216‑p
apoptosis, induced: Y216‑p
apoptosis, inhibited: S9‑p
cell adhesion, altered: S9‑p
cell differentiation, induced: S9‑p
cell growth, altered: S9‑p, Y216‑p
cell motility, altered: S9‑p
cytoskeletal reorganization: S9‑p
transcription, altered: S9‑p, Y216‑p
transcription, induced: Y216‑p
activity, inhibited: S9‑p
enzymatic activity, induced: Y216‑p
enzymatic activity, inhibited: S9‑p
intracellular localization: S9‑p, Y216‑p
molecular association, regulation: Y216‑p
phosphorylation: S9‑p
protein conformation: Y216‑p
protein degradation: S9‑p
protein stabilization: Y216‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 21 T7-p _MSGRPRttsFAEsC
0 22 T8-p MSGRPRttsFAEsCK
361 244 S9-p SGRPRttsFAEsCKP
0 5 S13-p RttsFAEsCKPVQQP
0 9 S21-p CKPVQQPsAFGsMKV
0 72 S25-p QQPsAFGsMKVSRDK
0 1 S35-p VSRDKDGsKVTTVVA
2 1 T43-p KVTTVVAtPGQGPDR
0 9 Y71-p NGSFGVVyQAKLCDS
1 0 K205 LCDFGSAKQLVRGEP
0 7 K205-ub LCDFGSAkQLVRGEP
0 1257 S215-p VRGEPNVsyICsRyy
65 4229 Y216-p RGEPNVsyICsRyyR
1 1118 S219-p PNVsyICsRyyRAPE
0 110 Y221-p VsyICsRyyRAPELI
0 7 Y222-p syICsRyyRAPELIF
0 3 K292-ub NPNYTEFkFPQIKAH
1 0 K292-sm NPNYTEFkFPQIKAH
2 8 S389-p ARIQAAAstPtNAtA
2 21 T390-p RIQAAAstPtNAtAA
0 1 T392-p QAAAstPtNAtAASD
0 5 T395-p AstPtNAtAASDANt
0 2 T402-p tAASDANtGDRGQTN
0 1 S417-p NAASASAsNST____
5558 : Phospho-GSK-3beta (Ser9) (D85E12) XP(TM) Rabbit mAb
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9322 : Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb
9323 : Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
9327 : Phospho-GSK-3alpha/beta (Ser21/9) (37F11) Rabbit mAb (GSK-3alpha Preferred)
9331 : Phospho-GSK-3alpha/beta (Ser21/9) Antibody
9336 : Phospho-GSK-3beta (Ser9) Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
3548 : Phospho GSK-3beta(Thr390) Antibody
  GSK3B iso2  
T7 _MSGRPRTTsFAESC
T8 MSGRPRTTsFAESCK
S9-p SGRPRTTsFAESCKP
S13 RTTsFAESCKPVQQP
S21 CKPVQQPSAFGSMKV
S25 QQPSAFGSMKVSRDK
S35 VSRDKDGSKVTTVVA
T43 KVTTVVATPGQGPDR
Y71 NGSFGVVYQAKLCDS
K205 LCDFGSAKQLVRGEP
K205 LCDFGSAKQLVRGEP
S215 VRGEPNVSyICSRYY
Y216-p RGEPNVSyICSRYYR
S219 PNVSyICSRYYRAPE
Y221 VSyICSRYYRAPELI
Y222 SyICSRYYRAPELIF
K292 NPNYTEFKFPQIKAH
K292 NPNYTEFKFPQIKAH
S402 ARIQAAASTPTNATA
T403 RIQAAASTPTNATAA
T405 QAAASTPTNATAASD
T408 ASTPTNATAASDANT
T415 TAASDANTGDRGQTN
S430 NAASASASNST____
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
  mouse

 
T7-p _MSGRPRttsFAEsC
T8-p MSGRPRttsFAEsCK
S9-p SGRPRttsFAEsCKP
S13-p RttsFAEsCKPVQQP
S21-p CKPVQQPsAFGsMKV
S25-p QQPsAFGsMKVSRDK
S35 VSRDKDGSKVTTVVA
T43 KVTTVVATPGQGPDR
Y71 NGSFGVVYQAKLCDS
K205 LCDFGSAKQLVRGEP
K205-ub LCDFGSAkQLVRGEP
S215-p VRGEPNVsyICsRYY
Y216-p RGEPNVsyICsRYYR
S219-p PNVsyICsRYYRAPE
Y221 VsyICsRYYRAPELI
Y222 syICsRYYRAPELIF
K292-ub NPNYTEFkFPQIKAH
K292 NPNYTEFKFPQIKAH
S389-p ARIQAAAsPPANAtA
P390 RIQAAAsPPANAtAA
A392 QAAAsPPANAtAASD
T395-p AsPPANAtAASDTNA
A402 tAASDTNAGDRGQTN
S417 NAASASASNST____
5558 : Phospho-GSK-3beta (Ser9) (D85E12) XP(TM) Rabbit mAb
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9322 : Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb
9323 : Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
9327 : Phospho-GSK-3alpha/beta (Ser21/9) (37F11) Rabbit mAb (GSK-3alpha Preferred)
9331 : Phospho-GSK-3alpha/beta (Ser21/9) Antibody
9336 : Phospho-GSK-3beta (Ser9) Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
  rat

 
T7 _MSGRPRTTsFAESC
T8 MSGRPRTTsFAESCK
S9-p SGRPRTTsFAESCKP
S13 RTTsFAESCKPVQQP
S21 CKPVQQPSAFGSMKV
S25 QQPSAFGSMKVSRDK
S35 VSRDKDGSKVTTVVA
T43 KVTTVVATPGQGPDR
Y71-p NGSFGVVyQAKLCDS
K205-ac LCDFGSAkQLVRGEP
K205 LCDFGSAKQLVRGEP
S215-p VRGEPNVsyICsRyY
Y216-p RGEPNVsyICsRyYR
S219-p PNVsyICsRyYRAPE
Y221-p VsyICsRyYRAPELI
Y222 syICsRyYRAPELIF
K292 NPNYTEFKFPQIKAH
K292 NPNYTEFKFPQIKAH
S389-p ARIQAAAsPPANATA
P390 RIQAAAsPPANATAA
A392 QAAAsPPANATAASD
T395 AsPPANATAASDTNA
A402 TAASDTNAGDRGQTN
S417 NAASASASNST____
5558 : Phospho-GSK-3beta (Ser9) (D85E12) XP(TM) Rabbit mAb
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9322 : Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb
9323 : Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
9327 : Phospho-GSK-3alpha/beta (Ser21/9) (37F11) Rabbit mAb (GSK-3alpha Preferred)
9331 : Phospho-GSK-3alpha/beta (Ser21/9) Antibody
9336 : Phospho-GSK-3beta (Ser9) Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
  rabbit

 
- gap
T2 ______XTSFAESXK
S3 _____XTSFAESXKP
S7 _XTSFAESXKPVQQP
S15 XKPVQQPSAFGS___
S19 QQPSAFGS_______
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
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