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Protein Page:
GRK2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GRK2 a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.15; Kinase, protein; Protein kinase, AGC; AGC group; GRK family; BARK subfamily
Cellular Component: membrane; cytoplasm; plasma membrane; cytosol
Molecular Function: G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity
Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; acetylcholine receptor signaling, muscarinic pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; innate immune response; positive regulation of catecholamine secretion; cardiac muscle contraction
Reference #:  P25098 (UniProtKB)
Alt. Names/Synonyms: ADRBK1; adrenergic, beta, receptor kinase 1; ARBK1; BARK; BARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; BETA-ARK1; FLJ16718; G-protein coupled receptor kinase 2; GRK2
Gene Symbols: ADRBK1
Molecular weight: 79,574 Da
Basal Isoelectric point: 6.89  Predict pI for various phosphorylation states
CST Pathways:  GPCR Signaling to MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GRK2

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell cycle regulation: S670‑p
cell growth, altered: S670‑p
activity, induced: S29‑p
molecular association, regulation: Y13‑p, Y86‑p, Y92‑p, S670‑p, S685‑p
protein degradation: Y13‑p, Y86‑p, Y92‑p, S670‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
5 0 Y13-p AVLADVSyLMAMEKS
2 0 S29-p ATPAARAsKKILLPE
0 1 T54-p LEDRGEVtFEkIFSQ
0 1 K57-ub RGEVtFEkIFSQkLG
0 3 K62-ub FEkIFSQkLGYLLFR
5 0 Y86-p ARPLVEFyEEIKKyE
5 0 Y92-p FyEEIKKyEKLETEE
0 1 K170-ub QKFIESDkFTRFCQW
0 1 S247-p RIMLSLVstGDCPFI
0 1 T248-p IMLSLVstGDCPFIV
0 1 T263-p CMSYAFHtPDKLSFI
0 4 K344-ub GLACDFSkkKPHASV
0 1 K345-ub LACDFSkkKPHASVG
0 71 Y356-p ASVGTHGyMAPEVLQ
0 1 S416-p MAVELPDsFSPELRS
0 3 S487 ADAFDIGSFDEEDTK
0 1 Y506-p LDSDQELyRNFPLTI
0 1 K628-ub LLKIRGGkQFILQCD
0 1 K644-ac DPELVQWkKELRDAY
3 36 S670-p KMKNKPRsPVVELSk
0 1 S676 RsPVVELSkVPLVQR
0 1 K677-ub sPVVELSkVPLVQRG
2 5 S685-p VPLVQRGsANGL___
  mouse

 
Y13-p AVLADVSyLMAMEKS
S29 ATPAARASKKILLPE
T54 LEDRGEVTFEKIFSQ
K57 RGEVTFEKIFSQKLG
K62 FEKIFSQKLGYLLFR
Y86-p AKPLVEFyEEIKKyE
Y92-p FyEEIKKyEKLETEE
K170 QKFIESDKFTRFCQW
S247 RIMLSLVSTGDCPFI
T248 IMLSLVSTGDCPFIV
T263 CMSYAFHTPDKLSFI
K344-ub GLACDFSkKRPHASV
K345 LACDFSkKRPHASVG
Y356-p ASVGTHGyMAPEVLQ
S416 MAVELPDSFSPELRS
S487-p ADAFDIGsFDEEDTK
Y506-p LDSDQELyRNFPLTI
K628 LLKIRGGKQFVLQCD
K644 DPELVQWKKELRDAY
S670-p KMKNKPRsPVVELsK
S676-p RsPVVELsKVPLIQR
K677 sPVVELsKVPLIQRG
S685 VPLIQRGSANGL___
  rat

 
Y13 AVLADVSYLMAMEKS
S29-p ATPAARAsKKILLPE
T54 LEDRGEVTFEKIFSQ
K57 RGEVTFEKIFSQKLG
K62 FEKIFSQKLGYLLFR
Y86 AKPLVEFYEEIEKYE
Y92 FYEEIEKYEKLETEE
K170 HKFIESDKFTRFCQW
S247 RIMLSLVSTGDCPFI
T248 IMLSLVSTGDCPFIV
T263 CMSYAFHTPDKLSFI
K344 GLACDFSKKKPHASV
K345 LACDFSKKKPHASVG
Y356 ASVGTHGYMAPEVLQ
S416 MAVELPDSFSPELRS
S487 ADAFDIGSFDEEDTK
Y506 LDSDQELYRNFPLTI
K628 LLKIRGGKQFVLQCD
K644 DPELVQWKKELRDAY
S670 KMKNKPRSPVVELSK
S676 RSPVVELSKVPLIQR
K677 SPVVELSKVPLIQRG
S685 VPLIQRGSANGL___
  cow

 
Y13-p AVLADVSyLMAMEKS
S29 ATPAARASKKILLPE
T54 LEDRGEVTFEKIFSQ
K57 RGEVTFEKIFSQKLG
K62 FEKIFSQKLGYLLFR
Y86-p AKPLVEFyEEIKKyE
Y92-p FyEEIKKyEKLETEE
K170 QKFIESDKFTRFCQW
S247 RIMLSLVSTGDCPFI
T248 IMLSLVSTGDCPFIV
T263 CMSYAFHTPDKLSFI
K344 GLACDFSKKKPHASV
K345 LACDFSKKKPHASVG
Y356 ASVGTHGYMAPEVLQ
S416 MAVELPDSFSPELRS
S487 ADAFDIGSFDEEDTK
Y506 LDSDQELYRNFPLTI
K628 LLKIRGGKQFVLQCD
K644 DPELVQWKKELRDAY
S670-p KMKNKPRsPVVELSK
S676 RsPVVELSKVPLIQR
K677 sPVVELSKVPLIQRG
S685-p VPLIQRGsANGL___
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