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GRK2 (human)

Overview GRK2 a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function. Note: This description may include information from UniProtKB. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.15; Protein kinase, AGC; Kinase, protein; AGC group; GRK family; BARK subfamily Cellular Component: membrane; axon; basolateral plasma membrane; cytoplasm; apical plasma membrane; dendritic spine; plasma membrane; synapse; dendritic shaft; caveola; cytosol Molecular Function: G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; phospholipid binding; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity Biological Process: epidermal growth factor receptor signaling pathway; tachykinin signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; fibroblast growth factor receptor signaling pathway; acetylcholine receptor signaling, muscarinic pathway; follicle-stimulating hormone signaling pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; response to organic cyclic substance; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; response to hydrogen peroxide; phospholipase C activation; receptor internalization; positive regulation of catecholamine secretion; aging; cardiac muscle contraction Reference #:  P25098 (UniProtKB) Alt. Names/Synonyms: ADRBK1; adrenergic, beta, receptor kinase 1; ARBK1; BARK; BARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; BETA-ARK1; FLJ16718; G-protein coupled receptor kinase 2; GRK2 Gene Symbols: ADRBK1 Molecular weight: 79,574 Da Basal Isoelectric point: 6.89  Predict pI for various phosphorylation states CST Pathways:  GPCRs Signaling to MAPK/Erk Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below GRK2 1BAK - A=556-670 (human) 3CIK - A=1-689 (human) 3KRW - A=2-689 (human) 3KRX - A=2-689 (human) 3V5W - A=1-689 (human) 1OMW - A=1-689 (cow) 1YM7 - A/B/C/D=1-689 (cow) 2BCJ - A=1-689 (cow) 3PSC - A=1-689 (cow) 3PVU - A=1-689 (cow) 3PVW - A=1-689 (cow) 3UZS - A=1-689 (cow) 3UZT - A=1-689 (cow) Substrate Sequence Logo

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1BAK 3CIK 3KRW 3KRX 3V5W  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
cell cycle regulation: S670‑p cell growth, altered: S670‑p activation: S29‑p molecular association, regulation: Y13‑p, Y86‑p, Y92‑p, S670‑p, S685‑p protein degradation: Y13‑p, Y86‑p, Y92‑p, S670‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
5	0		Y13-p	AVLADVSyLMAMEKS
2	0		S29-p	ATPAARAsKKILLPE
0	1		T54-p	LEDRGEVtFEkIFSQ
0	1		K57-u	RGEVtFEkIFSQkLG
0	3		K62-u	FEkIFSQkLGYLLFR
5	0		Y86-p	ARPLVEFyEEIKKyE
5	0		Y92-p	FyEEIKKyEKLETEE
0	1		K170-u	QKFIESDkFTRFCQW
0	1		S247-p	RIMLSLVstGDCPFI
0	1		T248-p	IMLSLVstGDCPFIV
0	1		T263-p	CMSYAFHtPDKLSFI
0	4		K344-u	GLACDFSkkKPHASV
0	1		K345-u	LACDFSkkKPHASVG
0	70		Y356-p	ASVGTHGyMAPEVLQ
0	1		S416-p	MAVELPDsFSPELRS
0	3		S487	ADAFDIGSFDEEDTK
0	1		Y506-p	LDSDQELyRNFPLTI
0	1		K628-u	LLKIRGGkQFILQCD
0	1		K644-a	DPELVQWkKELRDAY
3	29		S670-p	KMKNKPRsPVVELSk
0	1		S676	RsPVVELSkVPLVQR
0	1		K677-u	sPVVELSkVPLVQRG
2	3		S685-p	VPLVQRGsANGL___

	mouse
Y13-p	AVLADVSyLMAMEKS
S29	ATPAARASKKILLPE
T54	LEDRGEVTFEKIFSQ
K57	RGEVTFEKIFSQKLG
K62	FEKIFSQKLGYLLFR
Y86-p	AKPLVEFyEEIKKyE
Y92-p	FyEEIKKyEKLETEE
K170	QKFIESDKFTRFCQW
S247	RIMLSLVSTGDCPFI
T248	IMLSLVSTGDCPFIV
T263	CMSYAFHTPDKLSFI
K344-u	GLACDFSkKRPHASV
K345	LACDFSkKRPHASVG
Y356-p	ASVGTHGyMAPEVLQ
S416	MAVELPDSFSPELRS
S487-p	ADAFDIGsFDEEDTK
Y506-p	LDSDQELyRNFPLTI
K628	LLKIRGGKQFVLQCD
K644	DPELVQWKKELRDAY
S670-p	KMKNKPRsPVVELsK
S676-p	RsPVVELsKVPLIQR
K677	sPVVELsKVPLIQRG
S685	VPLIQRGSANGL___

	rat
Y13	AVLADVSYLMAMEKS
S29-p	ATPAARAsKKILLPE
T54	LEDRGEVTFEKIFSQ
K57	RGEVTFEKIFSQKLG
K62	FEKIFSQKLGYLLFR
Y86	AKPLVEFYEEIEKYE
Y92	FYEEIEKYEKLETEE
K170	HKFIESDKFTRFCQW
S247	RIMLSLVSTGDCPFI
T248	IMLSLVSTGDCPFIV
T263	CMSYAFHTPDKLSFI
K344	GLACDFSKKKPHASV
K345	LACDFSKKKPHASVG
Y356	ASVGTHGYMAPEVLQ
S416	MAVELPDSFSPELRS
S487	ADAFDIGSFDEEDTK
Y506	LDSDQELYRNFPLTI
K628	LLKIRGGKQFVLQCD
K644	DPELVQWKKELRDAY
S670	KMKNKPRSPVVELSK
S676	RSPVVELSKVPLIQR
K677	SPVVELSKVPLIQRG
S685	VPLIQRGSANGL___

	cow
Y13-p	AVLADVSyLMAMEKS
S29	ATPAARASKKILLPE
T54	LEDRGEVTFEKIFSQ
K57	RGEVTFEKIFSQKLG
K62	FEKIFSQKLGYLLFR
Y86-p	AKPLVEFyEEIKKyE
Y92-p	FyEEIKKyEKLETEE
K170	QKFIESDKFTRFCQW
S247	RIMLSLVSTGDCPFI
T248	IMLSLVSTGDCPFIV
T263	CMSYAFHTPDKLSFI
K344	GLACDFSKKKPHASV
K345	LACDFSKKKPHASVG
Y356	ASVGTHGYMAPEVLQ
S416	MAVELPDSFSPELRS
S487	ADAFDIGSFDEEDTK
Y506	LDSDQELYRNFPLTI
K628	LLKIRGGKQFVLQCD
K644	DPELVQWKKELRDAY
S670-p	KMKNKPRsPVVELSK
S676	RsPVVELSKVPLIQR
K677	sPVVELSKVPLIQRG
S685-p	VPLIQRGsANGL___

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