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Protein Page:
SMC3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SMC3 Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation; DNA repair, damage
Cellular Component: cohesin complex; spindle pole; nuclear matrix; chromosome; cytosol; nucleoplasm; lateral element; cytoplasm; nucleolus; basement membrane; chromatin; nucleus; chromosome, pericentric region
Molecular Function: dynein binding; protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding
Biological Process: meiosis; mitosis; mitotic spindle organization and biogenesis; stem cell maintenance; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle; DNA repair; signal transduction; regulation of DNA replication
Reference #:  Q9UQE7 (UniProtKB)
Alt. Names/Synonyms: BAM; Bamacan; Basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; Chondroitin sulfate proteoglycan 6; chondroitin sulfate proteoglycan 6 (bamacan); Chromosome-associated polypeptide; CSPG6; hCAP; SMC protein 3; SMC-3; SMC3; SMC3L1; structural maintenance of chromosomes 3; Structural maintenance of chromosomes protein 3
Gene Symbols: SMC3
Molecular weight: 141,542 Da
Basal Isoelectric point: 6.77  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SMC3

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: S1067‑p, S1083‑p
phosphorylation: S1067‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 K26-ub IVDPFSSkHNVIVGR
0 2 T69-p LALLHEGtGPrVISA
0 1 R72-m1 LHEGtGPrVISAFVE
0 1 S85-p VEIIFDNsDNRLPID
2 169 K105-ac LRRVIGAkkDQYFLD
2 164 K106-ac RRVIGAkkDQYFLDK
0 1 K118-ub LDKKMVTkNDVMNLL
0 1 K140-ac SNPYYIVkQGkINQM
0 1 K140-ub SNPYYIVkQGkINQM
0 1 K143-ub YYIVkQGkINQMATA
0 1 R155-m1 ATAPDSQrLKLLREV
0 1 S175-p YDERKEEsIsLMkET
0 1 S177-p ERKEEsIsLMkETEG
0 1 K180-ub EEsIsLMkETEGKRE
0 1 K188-ub ETEGKREkINELLkY
0 9 K194-ub EkINELLkYIEERLH
0 5 Y213-p EKEELAQyQkWDKMR
0 1 K215-ac EELAQyQkWDKMRRA
0 1 K215-ub EELAQyQkWDKMRRA
0 6 Y225-p KMRRALEyTIYNQEL
0 1 R236-m1 NQELNETrAKLDELs
0 1 S243-p rAKLDELsAkRETSG
0 1 K245-m1 KLDELsAkRETSGEK
0 1 K252 kRETSGEKSRQLRDA
0 1 R272-m1 DKMEDIErQVRELKT
0 1 R323-m1 LAGNSEQrKRLLKER
0 1 K336-ac ERQKLLEkIEEKQKE
0 1 K409-m1 LDQAINDkKRQIAAI
0 1 K427-m1 LEDTEANkEkNLEQY
0 1 K429-m1 DTEANkEkNLEQYNK
0 1 K445-m1 DQDLNEVkARVEELD
0 1 K459 DRKYYEVKNKKDELQ
0 2 K461 KYYEVKNKKDELQSE
0 1 K462 YYEVKNKKDELQSER
0 2 K462 YYEVKNKKDELQSER
0 1 K486-m1 EQQALAAkREDLEKK
0 1 K486-ub EQQALAAkREDLEKK
0 50 Y560-p TAGNRLFyHIVDSDE
0 1 K592-ub VTFLPLNkLDVRDTA
0 4 Y600-p LDVRDTAyPETNDAI
0 1 K612-ub DAIPMISkLRyNPRF
0 4 Y615-p PMISkLRyNPRFDKA
0 1 R644-m1 EVSTQLArAFTMDCI
0 55 Y668-p RGALTGGyyDTRKsR
0 9 Y669-p GALTGGyyDTRKsRL
0 1 S674-p GyyDTRKsRLELQKD
0 1 S731-p QQRKFKAsRDSILsE
0 1 S737-p AsRDSILsEMKMLKE
0 1 T773-p SLHAMEStRESLKAE
0 1 T783-p SLKAELGtDLLsQLs
0 9 S787-p ELGtDLLsQLsLEDQ
0 1 S790-p tDLLsQLsLEDQkRV
0 4 K795-ac QLsLEDQkRVDALND
0 1 K795-ub QLsLEDQkRVDALND
0 1 Y831-p IITRVETyLNENLRK
0 3 S886-p RSEDLDNsIDKTEAG
0 1 T926-p TKELEKMtNRQGMLL
0 1 K956-ac LPQEAFEkYQTLSLk
0 1 K956-ub LPQEAFEkYQTLSLk
0 4 K963-ub kYQTLSLkQLFRKLE
0 1 Y979-p CNTELKKySHVNKkA
0 1 K985-ub KySHVNKkALDQFVN
0 1 K997-m1 FVNFSEQkEKLIKRQ
0 1 K997-ub FVNFSEQkEKLIKRQ
0 2 Y1011 QEELDRGYksIMELM
0 2 K1012-ub EELDRGYksIMELMN
0 2 S1013-p ELDRGYksIMELMNV
0 2 K1025-ub MNVLELRkYEAIQLT
0 14 K1034-ub EAIQLTFkQVSkNFS
0 1 K1038-ub LTFkQVSkNFSEVFQ
0 1 K1046-ub NFSEVFQkLVPGGKA
0 25 K1059-ub KATLVMKkGDVEGsQ
0 19 S1065-p KkGDVEGsQsQDEGE
1 62 S1067-p GDVEGsQsQDEGEGs
0 9 S1074-p sQDEGEGsGEsERGs
0 2 S1077-p EGEGsGEsERGsGsQ
0 4 S1081-p sGEsERGsGsQssVP
3 11 S1083-p EsERGsGsQssVPSV
0 2 S1085-p ERGsGsQssVPSVDQ
0 1 S1086-p RGsGsQssVPSVDQF
0 1 K1105-ub IRVSFTGkQGEMREM
0 1 S1116-p MREMQQLsGGQKSLV
0 1 K1190-ac ADKFYGVkFRNkVSH
0 1 K1190-ub ADKFYGVkFRNkVSH
0 1 K1194-ub YGVkFRNkVSHIDVI
  mouse

 
K26 IVDPFSSKHNVIVGR
T69 LALLHEGTGPRVISA
R72 LHEGTGPRVISAFVE
S85 VEIIFDNSDNRLPID
K105-ac LRRVIGAkkDQYFLD
K106-ac RRVIGAkkDQYFLDK
K118 LDKKMVTKNDVMNLL
K140 SNPYYIVKQGKINQM
K140 SNPYYIVKQGKINQM
K143 YYIVKQGKINQMATA
R155 ATAPDSQRLKLLREV
S175 YDERKEESISLMKET
S177 ERKEESISLMKETEG
K180 EESISLMKETEGKRE
K188 ETEGKREKINELLkY
K194-ub EKINELLkYIEERLH
Y213-p EKEELAQyQKWDKMR
K215 EELAQyQKWDKMRRA
K215 EELAQyQKWDKMRRA
Y225-p KMRRALEyTIYNQEL
R236 NQELNETRAKLDELS
S243 RAKLDELSAKRETSG
K245 KLDELSAKRETSGEk
K252-ac KRETSGEkSRQLRDA
R272 DKMEDIERQVRELKT
R323 LAGNSEQRKRLLKER
K336 ERQKLLEKIEEKQKE
K409 LDQAINDKKRQIAAI
K427 LEDTEANKEKNLEQY
K429 DTEANKEKNLEQYNK
K445 DQDLNEVKARVEELD
K459-ub DRKYYEVkNkkDELQ
K461-ac KYYEVkNkkDELQSE
K462-ub YYEVkNkkDELQSER
K462-ac YYEVkNkkDELQSER
K486 EQQALAAKREDLEKK
K486 EQQALAAKREDLEKK
Y560-p TAGNRLFyHIVDSDE
K592 VTFLPLNKLDVRDTA
Y600 LDVRDTAYPETNDAI
K612 DAIPMISKLRYNPRF
Y615 PMISKLRYNPRFDKA
R644 EVSTQLARAFTMDCI
Y668-p RGALTGGyYDTRKSR
Y669 GALTGGyYDTRKSRL
S674 GyYDTRKSRLELQKD
S731 QQRKFKASRDSILSE
S737 ASRDSILSEMKMLKE
T773 SLHAMESTRESLKAE
T783 SLKAELGTDLLsQLS
S787-p ELGTDLLsQLSLEDQ
S790 TDLLsQLSLEDQKRV
K795 QLSLEDQKRVDALND
K795 QLSLEDQKRVDALND
Y831 IITRVETYLNENLRK
S886 RSEDLDNSIDKTEAG
T926 TKELEKMTNRQGMLL
K956 LPQEAFEKYQTLSLK
K956 LPQEAFEKYQTLSLK
K963 KYQTLSLKQLFRKLE
Y979 CNTELKKYSHVNKKA
K985 KYSHVNKKALDQFVN
K997 FVNFSEQKEKLIKRQ
K997 FVNFSEQKEKLIKRQ
Y1011 QEELDRGYKsIMELM
K1012 EELDRGYKsIMELMN
S1013-p ELDRGYKsIMELMNV
K1025 MNVLELRKYEAIQLT
K1034 EAIQLTFKQVSKNFS
K1038 LTFKQVSKNFSEVFQ
K1046 NFSEVFQKLVPGGKA
K1059 KATLVMKKGDVEGsQ
S1065-p KKGDVEGsQsQDEGE
S1067-p GDVEGsQsQDEGEGs
S1074-p sQDEGEGsGEsERGs
S1077-p EGEGsGEsERGsGsQ
S1081-p sGEsERGsGsQsSVP
S1083-p EsERGsGsQsSVPSV
S1085-p ERGsGsQsSVPSVDQ
S1086 RGsGsQsSVPSVDQF
K1105 IRVSFTGKQGEMREM
S1116 MREMQQLSGGQKSLV
K1190 ADKFYGVKFRNKVSH
K1190 ADKFYGVKFRNKVSH
K1194 YGVKFRNKVSHIDVI
  rat

 
K26 IVDPFSSKHNVIVGR
T69 LALLHEGTGPRVISA
R72 LHEGTGPRVISAFVE
S85 VEIIFDNSDNRLPID
K105-ac LRRVIGAkkDQYFLD
K106-ac RRVIGAkkDQYFLDK
K118 LDKKMVTKNDVMNLL
K140 SNPYYIVKQGKINQM
K140 SNPYYIVKQGKINQM
K143 YYIVKQGKINQMATA
R155 ATAPDSQRLKLLREV
S175 YDERKEESISLMKET
S177 ERKEESISLMKETEG
K180 EESISLMKETEGKRE
K188 ETEGKREKINELLKY
K194 EKINELLKYIEERLH
Y213 EKEELAQYQKWDKMR
K215 EELAQYQKWDKMRRA
K215 EELAQYQKWDKMRRA
Y225 KMRRALEYTIYNQEL
R236 NQELNETRAKLDELS
S243 RAKLDELSAKRETSG
K245 KLDELSAKRETSGEK
K252 KRETSGEKSRQLRDA
R272 DKMEDIERQVRELKT
R323 LAGNSEQRKRLLKER
K336 ERQKLLEKIEEKQKE
K409 LDQAINDKKRQIAAI
K427 LEDTEANKEKNLEQY
K429 DTEANKEKNLEQYNK
K445 DQDLNEVKARVEELD
K459 DRKYYEVKNKKDELQ
K461 KYYEVKNKKDELQSE
K462 YYEVKNKKDELQSER
K462 YYEVKNKKDELQSER
K486 EQQALAAKREDLEKK
K486 EQQALAAKREDLEKK
Y560 TAGNRLFYHIVDSDE
K592 VTFLPLNKLDVRDTA
Y600 LDVRDTAYPETNDAI
K612 DAIPMISKLRYNPRF
Y615 PMISKLRYNPRFDKA
R644 EVSTQLARAFTMDCI
Y668 RGALTGGYYDTRKSR
Y669 GALTGGYYDTRKSRL
S674 GYYDTRKSRLELQKD
S731 QQRKFKASRDSTLSE
S737 ASRDSTLSEMKMLKE
T773 SLHAMESTRESLKAE
T783 SLKAELGTDLPSQLS
S787 ELGTDLPSQLSLEDQ
S790 TDLPSQLSLEDQKRV
K795 QLSLEDQKRVDALND
K795 QLSLEDQKRVDALND
Y831 IITRVETYLNENLRK
S886 RSEDLDNSIDKTEAG
T926 TKELEKMTNRQGMLL
K956 LPQEAFEKYQTLSLK
K956 LPQEAFEKYQTLSLK
K963 KYQTLSLKQLFRKLE
Y979 CNTELKKYSHVNKKA
K985 KYSHVNKKALDQFVN
K997 FVNFSEQKEKLIKRQ
K997 FVNFSEQKEKLIKRQ
Y1011-p QEELDRGyKSIMELM
K1012 EELDRGyKSIMELMN
S1013 ELDRGyKSIMELMNV
K1025 MNVLELRKYEAIQLT
K1034 EAIQLTFKQVSKNFS
K1038 LTFKQVSKNFSEVFQ
K1046 NFSEVFQKLVPGAKA
K1059 KATLVMKKGDVEGsQ
S1065-p KKGDVEGsQsQDEGE
S1067-p GDVEGsQsQDEGEGS
S1074 sQDEGEGSGESERGS
S1077 EGEGSGESERGSGSQ
S1081 SGESERGSGSQSSVP
S1083 ESERGSGSQSSVPSV
S1085 ERGSGSQSSVPSVDQ
S1086 RGSGSQSSVPSVDQF
K1105 IRVSFTGKQGEMREM
S1116 MREMQQLSGGQKSLV
K1189 SADKSSGKSE_____
K1189 SADKSSGKSE_____
- gap
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