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Protein Page:
TPX2 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
TPX2 a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation
Cellular Component: microtubule cytoskeleton; spindle pole; microtubule; cytoplasm; nucleolus; spindle; nucleus
Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding
Biological Process: cell proliferation; mitosis; apoptosis; cell division; activation of protein kinase activity
Reference #:  Q9ULW0 (UniProtKB)
Alt. Names/Synonyms: C20orf1; C20orf2; Differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; Hepatocellular carcinoma-associated antigen 519; p100; preferentially expressed in colorectal cancer; Protein fls353; REPP86; restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for Xklp2; TPX2; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog (Xenopus laevis)
Gene Symbols: TPX2
Molecular weight: 85,653 Da
Basal Isoelectric point: 9.29  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TPX2
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 4 K44-u EKANLENkLLGkNGt
0 1 K48-u LENkLLGkNGtGGLF
1 0 - gap
0 1 T51-p kLLGkNGtGGLFQGK
0 1 T59-p GGLFQGKtPLRKANL
0 1 P60 GLFQGKtPLRKANLQ
1 31 T72-p NLQQAIVtPLkPVDN
0 1 K75-u QAIVtPLkPVDNTyy
0 1 Y81-p LkPVDNTyyKEAEKE
0 1 Y82-p kPVDNTyyKEAEKEN
0 2 S110-p LEVEAAIsRKtPAQP
0 10 T113-p EAAIsRKtPAQPQRR
1 42 S121-p PAQPQRRsLRLsAQK
1 27 S125-p QRRsLRLsAQKDLEQ
0 32 T147-p MKAKRCAtPVIIDEI
0 1 S157-p IIDEILPskKMKVSN
0 12 K158-a IDEILPskKMKVSNN
0 1 S185-p DTAEKNAssPEKAKG
0 2 S186-p TAEKNAssPEKAKGR
0 3 S218-p EEQELEKsMKMQQEV
0 1 S252-p QPVKKSVsQVTKsVD
0 2 S257-p SVsQVTKsVDFHFRT
0 2 Y278-p HPKNQEEyKEVNFTS
0 1 K289 NFTSELRKHPssPAR
0 4 S292-p SELRKHPssPARVTK
0 17 S293-p ELRKHPssPARVTKG
0 3 K305-a TKGCTIVkPFNLsQG
0 6 S310-p IVkPFNLsQGkkRtF
0 5 K313-a PFNLsQGkkRtFDET
0 2 K314-a FNLsQGkkRtFDETV
0 1 T316-p LsQGkkRtFDETVST
0 3 Y324-p FDETVSTyVPLAQQV
0 4 T338-p VEDFHKRtPNRYHLR
0 1 S356-p DDINLLPsKSsVTKI
0 1 S359-p NLLPsKSsVTKICRD
1 102 T369-p KICRDPQtPVLQtKH
0 2 T374-p PQtPVLQtKHRARAV
0 1 T382-p KHRARAVtCKStAEL
0 1 T386-p RAVtCKStAELEAEE
0 2 K420-u EGGPILPkkPPVKPP
0 2 K421-u GGPILPkkPPVKPPT
0 1 T482-p EKKVLPItVPKsPAF
0 120 S486-p LPItVPKsPAFALKN
0 109 T499-p KNRIRMPtkEDEEED
0 3 K500-a NRIRMPtkEDEEEDE
0 42 Y519-p KAQPVPHyGVPFkPQ
0 1 K524-u PHyGVPFkPQIPEAR
0 1 S542-p ICPFSFDsRDKERQL
0 1 K567-u KGEVPKFkALPLPHF
0 1 S634-p ARPNTVIsQEPFVPK
0 2 S646-p VPKKEKKsVAEGLsG
0 1 S652-p KsVAEGLsGsLVQEP
0 1 S654-p VAEGLsGsLVQEPFQ
0 2 Y721-p KANPIRKyQGLEIKs
0 1 S728-p yQGLEIKsSDQPLTV
1 38 S738-p QPLTVPVsPKFSTRF
  mouse

 
K44-u EKANLENkFLRQRGI
Q48 LENkFLRQRGIGEPF
- gap
I51 kFLRQRGIGEPFQGK
N59 GEPFQGKNsLRKAKL
S60-p EPFQGKNsLRKAKLQ
T72-p KLQQGFVtPLKAVDN
K75 QGFVtPLKAVDNTYH
Y81 LKAVDNTYHKETEKE
H82 KAVDNTYHKETEKEN
S110 LDAKRAVSGNtPVQP
T113-p KRAVSGNtPVQPQRR
S121-p PVQPQRRsIRLsAQK
S125-p QRRsIRLsAQKDLEQ
V149 EMKAKRCVAPATDCP
Q158 PATDCPPQKRMKVSH
K159 ATDCPPQKRMKVSHK
E187 TSRKNERETLEKAKG
T188 SRKNERETLEKAKGK
R220 EEQEIEKRLRMQQEV
S254 QPVKKSTSQVTKTVD
T259 STSQVTKTVDFHFLT
Y280 HPKNQEEYKEVNFMS
K291-a NFMSELRkHSSTPAR
S294 SELRkHSSTPARGTR
T295 ELRkHSSTPARGTRG
K307 TRGCTIIKPFNLSKG
S312 IIKPFNLSKGKKRTF
K315 PFNLSKGKKRTFDEA
K316 FNLSKGKKRTFDEAA
T318 LSKGKKRTFDEAAST
Y326 FDEAASTYVPIAQQV
T340 VEAFHKRTPNRYHLR
S357 KDESLLPSKSVNKIA
- gap
T369-p KIARDPQtPILQtKY
T374-p PQtPILQtKYRTRAV
T382 KYRTRAVTCKSTAEQ
T386 RAVTCKSTAEQEAEE
K420 ESGPILPKRAPVKPP
R421 SGPILPKRAPVKPPT
T482 EKKVIPATVPKsPVF
S486-p IPATVPKsPVFALKN
I499 KNRIRVPIKDEEEEK
K500 NRIRVPIKDEEEEKP
Y518 KAQPVPHYGVPYKPH
K523 PHYGVPYKPHIAEAR
T541 VCPFSFDTRDKERQL
K566 KGEVPKFKALPVPHF
F633 ARPNTVIFQEPFVPK
S645-p VPKKEKKsLAENPSG
S651 KsLAENPSGSLVQEP
S653 LAENPSGSLVQEPFQ
Y720 KANPIRKYAAVEVKS
S727 YAAVEVKSSELPLTV
S737-p LPLTVPVsPKFSTRF
  rat

 
K44 EKANLENKFLRQSGI
Q48 LENKFLRQSGIGEPF
- gap
I51 KFLRQSGIGEPFQGK
T59 GEPFQGKTSLRKAKL
S60 EPFQGKTSLRKAKLQ
T72 KLQQGCVTPLKAVDN
K75 QGCVTPLKAVDNTYY
Y81 LKAVDNTYYKETEKE
Y82 KAVDNTYYKETEKEN
T110 LNAKRAVTGTTPIQP
T113 KRAVTGTTPIQPQRR
S121 PIQPQRRSVRLSAQK
S125 QRRSVRLSAQKDLEQ
T149 EMKAKRCTAPTTDYP
N158 PTTDYPPNKKMKVSH
K159 TTDYPPNKKMKVSHK
E185 PATSRKDETLERAKG
T186 ATSRKDETLERAKGK
S218 EEQELEKSLRMQQEV
S252 QPVKKSTSQVTKTVD
T257 STSQVTKTVDFHFLT
Y278 HPKSQEEYKEVNFMS
K289 NFMSELRKHSSTPAR
S292 SELRKHSSTPARGTR
T293 ELRKHSSTPARGTRG
K305 TRGCTIIKPFNLSKG
S310 IIKPFNLSKGKKRTF
K313 PFNLSKGKKRTFDEA
K314 FNLSKGKKRTFDEAA
T316 LSKGKKRTFDEAAST
Y324 FDEAASTYVPIAQQV
T338 VEAFHKRTPNRYHLR
S355 KDESLLPSKPVNKIV
- gap
T367 KIVRDPQTPILQTKY
T372 PQTPILQTKYRARAV
T380 KYRARAVTCKSAAEK
A384 RAVTCKSAAEKEAEE
K418 ESGPILPKRPPVKPP
R419 SGPILPKRPPVKPPT
T480 EKKVIPATVPKSPVF
S484 IPATVPKSPVFALKN
V497 KNRIRVPVKEDEEEE
K498 NRIRVPVKEDEEEEK
Y517 KAQPVPHYGVPYKPP
K522 PHYGVPYKPPIPETR
T540 VCPFSFDTRDKERQL
K565 KGEVPKFKALPVPHF
F632 ARPNTVIFQEPFVPK
S644 VPKKEKKSAAENPSG
S650 KSAAENPSGSLVQEP
S652 AAENPSGSLVQEPFQ
Y719-p KANPIRKySAVEVKS
S726 ySAVEVKSSELPLTV
S736 LPLTVPVSPKFSTRF
  frog

 
- gap
Q44 AENIPPDQRRLsETS
S48-p PPDQRRLsETSVNTE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S84 THVCDVKSQSKRSsR
S90-p KSQSKRSsRRMsKKH
S94-p KRSsRRMsKKHRQKL
T114 DTHLEKETAPPEYPP
C122 APPEYPPCKKLKGSS
K123 PPEYPPCKKLKGSSS
M149 STSSHHSMTSPKPKA
T150 TSSHHSMTSPKPKAQ
M187 EEQELEKMQELQKEM
- gap
P225 TFIPVTKPVDFHFKT
Y248 NQPEGDGYKAVDFAS
K259 DFASELRKHPPSPVQ
P262 SELRKHPPSPVQVTK
S263 ELRKHPPSPVQVTKG
K276 KGGHTVPKPFNLSKG
S281 VPKPFNLSKGKRKHE
K284 PFNLSKGKRKHEEAS
R285 FNLSKGKRKHEEASD
K286 NLSKGKRKHEEASDY
Y293 KHEEASDYVSTAEQV
T307 VIAFYKRTPARYHLR
S324 QREMEGPSPVKMIKT
I329 GPSPVKMIKTKLTNP
T338 TKLTNPKTPLLQTKG
T343 PKTPLLQTKGRHRPV
T351 KGRHRPVTCKSAAEL
A355 RPVTCKSAAELEAEE
K389 EGGPVLLKKPLVKEP
K390 GGPVLLKKPLVKEPT
T451 LKKLLPVTVPQSPAF
S455 LPVTVPQSPAFALKN
A468 KNRVRIPAQEEKQEE
Q469 NRVRIPAQEEKQEEM
Y488 KATRMPHYGVPFKPK
K493 PHYGVPFKPKLVEQR
D511 VCPFSFCDRDKERQL
K536 KDEVPKFKAQPLPQF
H603 ARPNTVVHQEPFVPK
S615 VPKKENRSLTESLSG
S621 RSLTESLSGSIVQEG
S623 LTESLSGSIVQEGFE
Y690 KAKPIRKYRAVEVKA
A697 YRAVEVKASDVPLTV
S707 VPLTVPRSPNFSDRF
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