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DLAT (human)

Overview DLAT the E2 component of the pyruvate dehydrogenase complex (PDHC) that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. Contains two lipoyl-binding domains. The PDHC plays a major role in controlling the balance between lipid and glucose oxidation depending on substrate availability. The activity of the PDHC is tightly regulated by phosphorylation of the E1 components by the PDHKs. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homo-dimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to K174 of the inner lipoyl domain of the E2 component. Note: This description may include information from UniProtKB. Protein type: Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - citrate (TCA) cycle; Transferase; EC 2.3.1.12; Mitochondrial Cellular Component: mitochondrial matrix; mitochondrial pyruvate dehydrogenase complex Molecular Function: dihydrolipoyllysine-residue acetyltransferase activity; protein binding Biological Process: acetyl-CoA biosynthetic process from pyruvate; glycolysis; acetyl-CoA biosynthetic process; regulation of acetyl-CoA biosynthetic process from pyruvate; sleep; pyruvate metabolic process Reference #:  P10515 (UniProtKB) Alt. Names/Synonyms: 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoamide S-acetyltransferase; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; DLAT; DLTA; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; ODP2; PBC; PDC-E2; PDCE2; Pyruvate dehydrogenase complex component E2 Gene Symbols: DLAT Molecular weight: 68,997 Da Basal Isoelectric point: 7.96  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below DLAT 1FYC - A=179-282 (human) 2DNE - A=59-153 (human) 3B8K - A=376-614 (human)

STRING  |  Scansite  |  Phospho.ELM  |  Pfam  |  RCSB PDB 1FYC 2DNE 3B8K  |  DISEASE 245348 608770  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	2		T35-p	VPGTPRVtSRSGPAP
0	1		Y52	RNSVTTGYGGVRALC
0	1		-	under review
0	1		A67	GWTPSSGATPRNRLL
0	2		S80-p	LLLQLLGsPGRRYYS
0	1		K93-a	YSLPPHQkVPLPSLs
0	1		S100-p	kVPLPSLsPtMQAGT
0	1		T102-p	PLPSLsPtMQAGTIA
0	1		T154-p	KILVAEGtRDVPIGA
0	1		Y271-p	FEVQEEGyLAKILVP
0	1		T281-p	KILVPEGtRDVPLGT
0	1		T312-p	DYRPTEVtDLKPQVP
0	2		S358-p	PKGRVFVsPLAkkLA
0	4		K362-a	VFVsPLAkkLAVEKG
0	1		K362	VFVsPLAKkLAVEKG
0	1		K363	FVsPLAkKLAVEKGI
0	1		K363-u	FVsPLAkkLAVEKGI
0	1		K368	AkkLAVEKGIDLTQV
0	2		K376-a	GIDLTQVkGTGPDGR
0	1		K376-u	GIDLTQVkGTGPDGR
0	1		S391-p	ITKKDIDsFVPSKVA
0	17		K462-a	GEVLLVRkELNkILE
0	19		K466-a	LVRkELNkILEGRsK
0	1		S472-p	NkILEGRsKIsVNDF
0	1		K473	kILEGRsKIsVNDFI
0	4		S475-p	LEGRsKIsVNDFIIK
0	2		K547	DVVSLATKAREGKLQ
0	10		R636	AQWLAEFRkYLEKPI
0	2		K637-a	QWLAEFRkYLEKPIT
0	1		K641	EFRkYLEKPITMLL_

	mouse
S35-p	GPGAPCGsPRIGPAA
Y52-p	CGSGIPRyGVRsLCG
S56-p	IPRyGVRsLCGWSSG
T66-p	GWSSGSGtVPRNRLL
S79	LLRQLLGSPSRRSYS
K92	YSLPPHQKVPLPSLS
S99	KVPLPSLSPTMQAGT
T101	PLPSLSPTMQAGTIA
T153	KILVPEGTRDVPVGS
Y270	FEVQEEGYLAKILVP
T280	KILVPEGTRDVPLGA
T311	DYRPTEVTSLKPQAA
S353	PKGRVFVSPLAkkLA
K357	VFVSPLAKkLAAEkG
K357-m1	VFVSPLAkkLAAEkG
K358-m1	FVSPLAkkLAAEkGI
K358	FVSPLAkKLAAEkGI
K363-m1	AkkLAAEkGIDLTQV
K371-a	GIDLTQVkGTGPEGR
K371	GIDLTQVKGTGPEGR
S386	IIKKDIDSFVPSKAA
K457	GEVLLVRKELNkMLE
K461-a	LVRKELNkMLEGKGk
G467	NkMLEGKGkIsVNDF
K468-u	kMLEGKGkIsVNDFI
S470-p	LEGKGkIsVNDFIIK
K542	DVVSLASKAREGKLQ
K631-a	AQWLAEFkkYLEkPI
K632-a	QWLAEFkkYLEkPIT
K636-u	EFkkYLEkPITMLL_

	rat
-	under review
Y44	CGSGIPSYGVRSLCG
S48	IPSYGVRSLCGWSYG
T58	GWSYGSATVPRNRIL
S71	ILQQLLGSPSRRSYS
K84	YSLPPHQKVPLPSLS
S91	KVPLPSLSPTMQAGT
T93	PLPSLSPTMQAGTIA
T145	KILVPEGTRDVPVGS
Y261	FEVQEEGYLAKILVP
T271	KILVPEGTRDVPLGT
T302	DYRPTEVTSLKPQAP
S344	PKGRVFVSPLAKKLA
K348	VFVSPLAKKLAAEKG
K348	VFVSPLAKKLAAEKG
K349	FVSPLAKKLAAEKGI
K349	FVSPLAKKLAAEKGI
K354	AKKLAAEKGIDLTQV
K362	GIDLTQVKGTGPEGR
K362	GIDLTQVKGTGPEGR
S377	IIKKDIDSFVPTKAA
K447	GEVLLVRKELNKMLE
K451	LVRKELNKMLEGKGK
G457	NKMLEGKGKISVNDF
K458	KMLEGKGKISVNDFI
S460	LEGKGKISVNDFIIK
K532-a	DVVSLASkAREGKLQ
K621-a	AQWLAEFkKYLEKPV
K622	QWLAEFkKYLEKPVT
K626	EFkKYLEKPVTMLL_

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