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Protein Page:
Symplekin (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Symplekin Heat-labile component of a multimolecular complex that function in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. May be required for pre-mRNA polyadenylation. Found in a heat-sensitive complex at least composed of several cleavage and polyadenylation specific and cleavage stimulation factors. Interacts with CPSF2, CPSF3 and CSTF2. Interacts with HSF1 in heat-stressed cells. In testis, expressed in polar epithelia and Sertoli cells but not in vascular endothelia. The protein is detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens-forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells but not in endothelium of vessels, epidermis, intercalated disks, Purkinje fiber cells of the heart and lymph node. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; RNA processing
Cellular Component: nucleoplasm; tight junction; cytoskeleton; cytoplasm; plasma membrane; nucleolus; nucleus
Molecular Function: protein binding
Biological Process: mRNA polyadenylation; cell adhesion; positive regulation of protein amino acid dephosphorylation
Reference #:  Q92797 (UniProtKB)
Alt. Names/Synonyms: FLJ27092; SPK; SYM; SYMPK; Symplekin
Gene Symbols: SYMPK
Molecular weight: 141,148 Da
Basal Isoelectric point: 5.82  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Symplekin

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S3 _____MASGsGDSVT
0 1 G4 ____MASGsGDSVTR
0 3 S5-p ___MASGsGDSVTRR
0 1 S8 MASGsGDSVTRRsVA
0 2 S13-p GDSVTRRsVAsQFFt
0 2 S16-p VTRRsVAsQFFtQEE
0 1 T20-p sVAsQFFtQEEGPGI
0 1 T132-p VVKKAILtMTQLyKV
0 1 Y137-p ILtMTQLyKVALQWM
0 4 S147-p ALQWMVKsRVISELQ
0 1 T193 FVEGLIVTLSPRMAD
0 1 S195 EGLIVTLSPRMADsE
0 1 S201-p LSPRMADsEIPRRQE
0 1 S353-p RKRPRDDsDSTLkKM
0 21 K358-ac DDsDSTLkKMkLEPN
1 1 K361-sm DSTLkKMkLEPNLGE
0 1 S386 PSGTSKASAQISGQS
0 1 S390 SKASAQISGQSDTDI
0 1 T395 QISGQSDTDITAEFL
1 0 K483-sm PKEEKVVktESVLIK
0 1 T484-p KEEKVVktESVLIKR
0 8 S494-p VLIKRRLsAQGQAIs
0 2 S501-p sAQGQAIsVVGsLsS
0 2 S505-p QAIsVVGsLsSMsPL
0 1 S507-p IsVVGsLsSMsPLEE
0 3 S510-p VGsLsSMsPLEEEAP
0 1 K520-ub EEEAPQAkRRPEPII
0 3 S547-p RKKIFRLsDVLkPLt
0 2 K551-ub FRLsDVLkPLtDAQV
0 1 T554-p sDVLkPLtDAQVEAM
0 1 K562 DAQVEAMKLGAVKRI
0 2 Y624-p DLAFAWLyQEyNAYL
0 2 Y627-p FAWLyQEyNAYLAAG
0 2 Y642-p ASGSLDKyEDCLIRL
0 1 K656-ub LLSGLQEkPDQKDGI
0 1 Y693-p CEDESRTyLGMstLR
0 2 S697-p SRTyLGMstLRDLIF
0 2 T698-p RTyLGMstLRDLIFK
0 1 K850 LLVENCPKGAETLVT
0 1 K866 CLHSLTDKVPPSPEL
0 1 K875 PPSPELVKRVRDLYH
0 1 K900 PVLNGLEKKEVIQAL
0 1 K901 VLNGLEKKEVIQALP
0 2 K909-ub EVIQALPkLIKLNPI
0 2 K919-ub KLNPIVVkEVFNRLL
0 1 K964 CDMKSIIKATNLCFA
0 1 S1021-p GFVMNILsRLIMKQV
0 30 Y1031-p IMKQVWKyPKVWEGF
0 1 K1040-ub KVWEGFIkCCQRTKP
0 1 S1168-p PGGVGAPssSsPsPs
0 1 S1169-p GGVGAPssSsPsPsP
0 3 S1171-p VGAPssSsPsPsPsA
0 1 S1173-p APssSsPsPsPsARP
0 7 S1175-p ssSsPsPsPsARPGP
0 1 S1177-p SsPsPsPsARPGPPP
0 1 S1185-p ARPGPPPsEEAMDFR
0 2 S1221-p TEAALLDssLEGPLP
0 2 S1222-p EAALLDssLEGPLPK
0 1 T1231-p EGPLPKEtAAGGLtL
0 1 T1237-p EtAAGGLtLkEERsP
0 1 K1239-ac AAGGLtLkEERsPQt
0 23 S1243-p LtLkEERsPQtLAPV
0 1 T1246-p kEERsPQtLAPVGED
0 2 A1248 ERsPQtLAPVGEDAM
0 1 A1254 LAPVGEDAMKtPsPA
0 17 T1257-p VGEDAMKtPsPAAED
0 20 S1259-p EDAMKtPsPAAEDAR
0 1 A1265 PsPAAEDAREPEAKG
0 1 S1274-p EPEAKGNs_______
  mouse

► Hide Isoforms
 
S3-p _____MAsssGDsVT
S4-p ____MAsssGDsVTR
S5-p ___MAsssGDsVTRR
S8-p MAsssGDsVTRRSVA
S13 GDsVTRRSVASQFFT
S16 VTRRSVASQFFTQEE
T20 SVASQFFTQEEGPSI
T132 VVKKAILTMTQLYKV
Y137 ILTMTQLYKVALQWM
S147 ALQWMVKSRVISDLQ
T193-p FVEGLIVtLsPRMAD
S195-p EGLIVtLsPRMADSE
S201 LsPRMADSEVPRRQE
T353 RKRPRDDTDSTLkKM
K358-ac DDTDSTLkKMKLEPN
K361 DSTLkKMKLEPNLGE
S386-p PSGTSKAsAQIsGQS
S390-p SKAsAQIsGQSDtDI
T395-p QIsGQSDtDITAEFL
K483 PKEEKVVKPESVLIK
P484 KEEKVVKPESVLIKR
S494-p VLIKRRLsVQGQAIS
S501 sVQGQAISVVGsQST
S505-p QAISVVGsQSTMSPL
S507 ISVVGsQSTMSPLEE
S510 VGsQSTMSPLEEEVP
K520 EEEVPQAKRRPEPII
S547 RKKIFRLSDVLKPLT
K551 FRLSDVLKPLTDAQV
T554 SDVLKPLTDAQVEAM
K562-ub DAQVEAMkLGAVKRI
Y624 DLAFAWLYQEYNAYL
Y627 FAWLYQEYNAYLAAG
Y642 TSGTLDKYEDCLICL
K656 LLSGLQEKPDQKDGI
Y693 CEDESRAYLGMSTLG
S697 SRAYLGMSTLGDLIF
T698 RAYLGMSTLGDLIFK
K850-ub LLVENCPkGAETLVT
K866-ub CLHSLTDkVPPSPEL
K875-ub PPSPELVkRVRDLYH
K900-ub PVLNGLEkkEVIQAL
K901-ub VLNGLEkkEVIQALP
K909-ub EVIQALPkLIKLNPI
K919-ub KLNPIVVkEVFNRLL
K964-ub CDMKSIIkATNLCFA
A1021 GFVMNILARLIMKQV
Y1031 IMKQVWKYPKVWEGF
K1040 KVWEGFIKCCQRTKP
A1178 PTGIGAPAACVSSTP
S1182 GAPAACVSSTPSVPA
T1184 PAACVSSTPSVPAAA
S1186 ACVSSTPSVPAAARA
P1188 VSSTPSVPAAARAGP
A1190 STPSVPAAARAGPTP
A1198 ARAGPTPAEEVMEYR
S1234 AETTLLDSSLEGPLP
S1235 ETTLLDSSLEGPLPK
A1244 EGPLPKEAAAVGSSS
S1250 EAAAVGSSSKDERsP
K1252 AAVGSSSKDERsPQN
S1256-p SSSKDERsPQNLSHA
N1259 KDERsPQNLSHAVEE
S1261 ERsPQNLSHAVEEAL
A1267 LSHAVEEALKtSsPE
T1270-p AVEEALKtSsPEtRE
S1272-p EEALKtSsPEtREPE
T1275-p LKtSsPEtREPESKG
S1284 EPESKGNS_______
  Symplekin iso3  
S3 _____MASSSGDSVT
S4 ____MASSSGDSVTR
S5 ___MASSSGDSVTRR
S8 MASSSGDSVTRRSVA
S13 GDSVTRRSVASQFFT
S16 VTRRSVASQFFTQEE
T20 SVASQFFTQEEGPSI
T132 VVKKAILTMTQLYKV
Y137 ILTMTQLYKVALQWM
S147 ALQWMVKSRVISDLQ
T193 FVEGLIVTLSPRMAD
S195 EGLIVTLSPRMADSE
S201 LSPRMADSEVPRRQE
T353 RKRPRDDTDSTLKKM
K358 DDTDSTLKKMKLEPN
K361 DSTLKKMKLEPNLGE
S386 PSGTSKASAQISGQS
S390 SKASAQISGQSDTDI
T395 QISGQSDTDITAEFL
K483 PKEEKVVKPESVLIK
P484 KEEKVVKPESVLIKR
S494 VLIKRRLSVQGQAIS
S501 SVQGQAISVVGSQST
S505 QAISVVGSQSTMSPL
S507 ISVVGSQSTMSPLEE
S510 VGSQSTMSPLEEEVP
K520 EEEVPQAKRRPEPII
S547 RKKIFRLSDVLKPLT
K551 FRLSDVLKPLTDAQV
T554 SDVLKPLTDAQVEAM
K562 DAQVEAMKLGAVKRI
Y624 DLAFAWLYQEYNAYL
Y627 FAWLYQEYNAYLAAG
Y642 TSGTLDKYEDCLICL
K656 LLSGLQEKPDQKDGI
Y693 CEDESRAYLGMSTLG
S697 SRAYLGMSTLGDLIF
T698 RAYLGMSTLGDLIFK
K850 LLVENCPKGAETLVT
K866 CLHSLTDKVPPSPEL
K875 PPSPELVKRVRDLYH
K900 PVLNGLEKKEVIQAL
K901 VLNGLEKKEVIQALP
K909 EVIQALPKLIKLNPI
K919 KLNPIVVKEVFNRLL
K964 CDMKSIIKATNLCFA
A1021 GFVMNILARLIMKQV
Y1031 IMKQVWKYPKVWEGF
K1040 KVWEGFIKCCQRTKP
A1176 PTGIGAPAACVSSTP
S1180 GAPAACVSSTPSVPA
T1182 PAACVSSTPSVPAAA
S1184 ACVSSTPSVPAAARA
P1186 VSSTPSVPAAARAGP
A1188 STPSVPAAARAGPTP
A1196 ARAGPTPAEEVMEYR
S1232 AETTLLDSSLEGPLP
S1233 ETTLLDSSLEGPLPK
A1242 EGPLPKEAAAVGSSS
S1248 EAAAVGSSSKDERsP
K1250 AAVGSSSKDERsPQN
S1254-p SSSKDERsPQNLsHA
N1257 KDERsPQNLsHAVEE
S1259-p ERsPQNLsHAVEEtL
T1265-p LsHAVEEtLKTSSPG
T1268 AVEEtLKTSSPGTRE
S1270 EEtLKTSSPGTREPE
T1273 LKTSSPGTREPESKG
S1282 EPESKGNS_______
  rat

 
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A46 GFVMNILARLIMKQV
Y56 IMKQVWKYPKVWEGF
K65 KVWEGFIKCCQRTKP
T199 PTGVGAPTSSVSSTP
S203 GAPTSSVSSTPLVGP
T205 PTSSVSSTPLVGPAA
L207 SSVSSTPLVGPAARA
G209 VSSTPLVGPAARAGP
A211 STPLVGPAARAGPTP
A219 ARAGPTPAEEVMEYR
S255 AETTLLDSSLEGPLP
S256 ETTLLDSSLEGPLPK
A265 EGPLPKEAAAVGPSS
S271 EAAAVGPSSKDERsP
K273 AAVGPSSKDERsPQN
S277-p PSSKDERsPQNLSHA
N280 KDERsPQNLSHAVEE
S282 ERsPQNLSHAVEEAL
A288 LSHAVEEALKTSSPE
T291 AVEEALKTSSPEARE
S293 EEALKTSSPEAREPE
A296 LKTSSPEAREPESKG
S305 EPESKGNS_______
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