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Protein Page:
NDRG1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NDRG1 a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D). Note: This description may include information from UniProtKB.
Protein type: Cell development/differentiation; Vesicle protein
Cellular Component: microtubule cytoskeleton; centrosome; microtubule; cell-cell adherens junction; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding
Biological Process: cell death; response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation
Reference #:  Q92597 (UniProtKB)
Alt. Names/Synonyms: CAP43; CMT4D; Differentiation-related gene 1 protein; DRG-1; DRG1; GC4; HMSNL; N-myc downstream regulated 1; N-myc downstream-regulated gene 1 protein; NDR1; NDRG1; Nickel-specific induction protein Cap43; NMSL; Protein NDRG1; protein regulated by oxygen-1; PROXY1; Reducing agents and tunicamycin-responsive protein; Rit42; RTP; TARG1; TDD5; tunicamycin-responsive protein
Gene Symbols: NDRG1
Molecular weight: 42,835 Da
Basal Isoelectric point: 5.49  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NDRG1

Protein Structure Not Found.


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Sites Implicated In
carcinogenesis, altered: S330‑p, T346‑p
carcinogenesis, inhibited: S330‑p, T346‑p
phosphorylation: S330‑p, T346‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 8 S2-p ______MsREMQDVD
0 4 K14-ub DVDLAEVkPLVEkGE
0 3 K19-ub EVkPLVEkGETITGL
0 6 K300-ub PQISQPAkLAEAFkY
0 1 K306-ub AkLAEAFkYFVQGMG
0 1 Y307 kLAEAFkYFVQGMGY
0 1 Y314 YFVQGMGYMPSAsMT
0 2 S317 QGMGYMPSAsMTRLM
0 3 S319-p MGYMPSAsMTRLMRs
0 47 S326-p sMTRLMRsRtAsGss
2 238 T328-p TRLMRsRtAsGssVt
6 488 S330-p LMRsRtAsGssVtsL
0 105 S332-p RsRtAsGssVtsLDG
0 257 S333-p sRtAsGssVtsLDGt
0 117 T335-p tAsGssVtsLDGtRs
0 261 S336-p AsGssVtsLDGtRsR
0 11 T340-p sVtsLDGtRsRsHts
1 129 S342-p tsLDGtRsRsHtsEG
0 71 S344-p LDGtRsRsHtsEGtR
12 242 T346-p GtRsRsHtsEGtRsR
0 51 S347-p tRsRsHtsEGtRsRs
0 30 T350-p RsHtsEGtRsRsHts
1 125 S352-p HtsEGtRsRsHtsEG
0 109 S354-p sEGtRsRsHtsEGtR
5 245 T356-p GtRsRsHtsEGtRsR
0 76 S357-p tRsRsHtsEGtRsRs
0 83 T360-p RsHtsEGtRsRsHts
1 320 S362-p HtsEGtRsRsHtsEG
0 201 S364-p sEGtRsRsHtsEGAH
6 530 T366-p GtRsRsHtsEGAHLD
0 183 S367-p tRsRsHtsEGAHLDI
0 122 T375-p EGAHLDItPNsGAAG
0 50 S378-p HLDItPNsGAAGNsA
0 1 A381 ItPNsGAAGNsAGPK
0 4 S384-p NsGAAGNsAGPKsME
0 11 S389-p GNsAGPKsMEVsC__
0 2 S393-p GPKsMEVsC______
3506 : Phospho-NDRG1 (Ser330) Antibody
11899 : Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb
3217 : Phospho-NDRG1 (Thr346) Antibody
5482 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb
6992 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
7497 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
8453 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8947 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (HRP Conjugate)
  mouse

 
S2-p ______MsRELHDVD
K14-ub DVDLAEVkPLVEKGE
K19 EVkPLVEKGESITGL
K300-ub PQISQPAkLAEAFKy
K306 AkLAEAFKyFVQGMG
Y307-p kLAEAFKyFVQGMGy
Y314-p yFVQGMGyMPsAsMT
S317-p QGMGyMPsAsMTRLM
S319-p MGyMPsAsMTRLMRs
S326-p sMTRLMRsRtAsGss
T328-p TRLMRsRtAsGssVt
S330-p LMRsRtAsGssVtsL
S332-p RsRtAsGssVtsLEG
S333-p sRtAsGssVtsLEGt
T335-p tAsGssVtsLEGtRs
S336-p AsGssVtsLEGtRsR
T340-p sVtsLEGtRsRsHts
S342-p tsLEGtRsRsHtsEG
S344-p LEGtRsRsHtsEGPR
T346-p GtRsRsHtsEGPRsR
S347-p tRsRsHtsEGPRsRs
P350 RsHtsEGPRsRsHts
S352-p HtsEGPRsRsHtsEG
S354-p sEGPRsRsHtsEGsR
T356-p GPRsRsHtsEGsRsR
S357-p PRsRsHtsEGsRsRs
S360-p RsHtsEGsRsRsHts
S362-p HtsEGsRsRsHtsED
S364-p sEGsRsRsHtsEDAR
T366-p GsRsRsHtsEDARLN
S367-p sRsRsHtsEDARLNI
T375-p EDARLNItPNsGAtG
S378-p RLNItPNsGAtGNNA
T381-p ItPNsGAtGNNAGPK
N384 NsGAtGNNAGPKSME
S389 GNNAGPKSMEVSC__
S393 GPKSMEVSC______
3506 : Phospho-NDRG1 (Ser330) Antibody
11899 : Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb
3217 : Phospho-NDRG1 (Thr346) Antibody
5482 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb
6992 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
7497 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
8453 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8947 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (HRP Conjugate)
  rat

 
S2 ______MSRELHDVD
K14 DVDLAEVKPLVEKGE
K19 EVKPLVEKGESITGL
K300 PQISQPAKLAEAFKY
K306 AKLAEAFKYFVQGMG
Y307 KLAEAFKYFVQGMGY
Y314 YFVQGMGYMPSASMT
S317 QGMGYMPSASMTRLM
S319 MGYMPSASMTRLMRS
S326 SMTRLMRSRtAsGSs
T328-p TRLMRSRtAsGSsVt
S330-p LMRSRtAsGSsVtsL
S332 RSRtAsGSsVtsLEG
S333-p SRtAsGSsVtsLEGT
T335-p tAsGSsVtsLEGTRs
S336-p AsGSsVtsLEGTRsR
T340 sVtsLEGTRsRSHts
S342-p tsLEGTRsRSHtsEG
S344 LEGTRsRSHtsEGPR
T346-p GTRsRSHtsEGPRsR
S347-p TRsRSHtsEGPRsRS
P350 RSHtsEGPRsRSHtS
S352-p HtsEGPRsRSHtSEG
S354 sEGPRsRSHtSEGsR
T356-p GPRsRSHtSEGsRsR
S357 PRsRSHtSEGsRsRs
S360-p RSHtSEGsRsRsHts
S362-p HtSEGsRsRsHtsED
S364-p SEGsRsRsHtsEDAR
T366-p GsRsRsHtsEDARLN
S367-p sRsRsHtsEDARLNI
T375 EDARLNITPSSGATG
S378 RLNITPSSGATGNNA
T381 ITPSSGATGNNAGPK
N384 SSGATGNNAGPKSME
S389 GNNAGPKSMEVSC__
S393 GPKSMEVSC______
3506 : Phospho-NDRG1 (Ser330) Antibody
3217 : Phospho-NDRG1 (Thr346) Antibody
5482 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb
6992 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
7497 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
8453 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate)
8947 : Phospho-NDRG1 (Thr346) (D98G11) XP(R) Rabbit mAb (HRP Conjugate)
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