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Protein Page:
eEF2K (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
eEF2K an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle Note: This description may include information from UniProtKB.
Protein type: Protein kinase, ATYPICAL; Kinase, protein; EC 2.7.11.20; Protein kinase, Ser/Thr (non-receptor); Translation; ATYPICAL group; Alpha family; eEF2K subfamily
Cellular Component: cytoplasm; cytosol
Molecular Function: calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity
Biological Process: translational elongation; protein amino acid autophosphorylation; regulation of protein amino acid autophosphorylation; insulin receptor signaling pathway
Reference #:  O00418 (UniProtKB)
Alt. Names/Synonyms: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; eEF-2 kinase; eEF-2K; EEF2K; EF2K; elongation factor-2 kinase; Eukaroytic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; MGC45041
Gene Symbols: EEF2K
Molecular weight: 82,144 Da
Basal Isoelectric point: 5.16  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  MAPK/Erk in Growth and Differentiation  |  Signaling Pathways Activating p38 MAPK  |  Translational Control
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

eEF2K
Protein Structure Not Found.


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Sites Implicated In
cell growth, induced: S78‑p
enzymatic activity, induced: S78‑p
enzymatic activity, inhibited: S78‑p, S359‑p
molecular association, regulation: S78‑p, S441‑p, S445‑p
protein degradation: S441‑p, S445‑p
ubiquitination: S441‑p, S445‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 12 S18-p EGVDGGQsPRAGHDG
0 6 S27-p RAGHDGDsDGDsDDE
0 3 G29 GHDGDsDGDsDDEEG
0 5 S31-p DGDsDGDsDDEEGyF
0 3 Y37-p DsDDEEGyFICPITD
0 11 Y59-p VNSKVNKyysNLTks
0 31 Y60-p NSKVNKyysNLTksE
1 2 S61-p SKVNKyysNLTksER
0 1 K65-u KyysNLTksERYsSs
1 0 S66-p yysNLTksERYsSsG
0 1 S70-p LTksERYsSsGsPAN
0 4 S71 TksERYsSsGsPANs
0 3 S72-p ksERYsSsGsPANsF
0 8 S74-p ERYsSsGsPANsFHF
0 2 N77 sSsGsPANsFHFKEA
5 1 S78-p SsGsPANsFHFKEAW
0 1 S135-p EVLIKMAsQPFGRGA
0 1 K162-u FLHAQQWkGASNYVA
0 1 Y236-p EHYIEGKyIkYNSNS
0 1 K238-u YIEGKyIkYNSNSGF
0 1 T340-p RDAVNQNtkLLQSAk
0 19 K341-u DAVNQNtkLLQSAkt
0 15 K347-u tkLLQSAktILRGtE
2 8 T348-p kLLQSAktILRGtEE
2 0 T353-p AktILRGtEEKCGsP
5 7 S359-p GtEEKCGsPQVRtLs
0 2 T364-p CGsPQVRtLsGSRPP
10 25 S366-p sPQVRtLsGSRPPLL
0 2 S368 QVRtLsGSRPPLLRP
3 4 S377-p PPLLRPLsENsGDEN
0 5 S380-p LRPLsENsGDENMSD
0 2 S386 NsGDENMSDVTFDsL
0 13 S392-p MSDVTFDsLPssPss
0 3 S395-p VTFDsLPssPssAtP
3 10 S396-p TFDsLPssPssAtPH
2 3 S398-p DsLPssPssAtPHSQ
0 2 S399-p sLPssPssAtPHSQK
0 1 T401-p PssPssAtPHSQKLD
0 1 S404 PssAtPHSQKLDHLH
0 1 S416-p HLHWPVFsDLDNMAS
0 1 S441-p ESENSGDsGyPsEKR
0 2 Y443-p ENSGDsGyPsEKRGE
2 12 S445-p SGDsGyPsEKRGELD
0 23 S470-p YSNRKYEsDEDsLGs
2 17 S474-p KYEsDEDsLGsSGRV
0 3 S477-p sDEDsLGsSGRVCVE
0 19 K485-u SGRVCVEkWNLLNsS
1 0 S491-p EkWNLLNsSRLHLPR
2 3 S500-p RLHLPRAsAVALEVQ
0 4 K516-u LNALDLEkkIGKSIL
0 1 K517-u NALDLEkkIGKSILG
0 1 Y600-p NKTKGFDyLLkAAEA
0 16 K603-u KGFDyLLkAAEAGDR
0 1 S627-p FDSGQNLsPDRCQDW
0 1 K684-u TGGYGLEkDPQRSGD
3691 : Phospho-eEF2k (Ser366) Antibody
  mouse

 
C18 EGVDGGRCSRAGHNA
S27-p RAGHNADsDtDsDDD
T29-p GHNADsDtDsDDDEG
S31-p NADsDtDsDDDEGYF
Y37 DsDDDEGYFICPITD
Y59 VSSKVQSYYSNLTKT
Y60 SSKVQSYYSNLTKTE
S61 SKVQSYYSNLTKTEC
K65 SYYSNLTKTECGstG
T66 YYSNLTKTECGstGs
G69 NLTKTECGstGsPAs
S70-p LTKTECGstGsPAss
T71-p TKTECGstGsPAssF
S73-p TECGstGsPAssFHF
S76-p GstGsPAssFHFKEA
S77-p stGsPAssFHFKEAW
S134 EVLIKMASQPFGRGA
K161 FLHAQQWKGASNYVA
Y235 EHYIEGKYIKYNSNS
K237 YIEGKYIKYNSNSGF
T339 QDAVNQSTRLLQSAK
R340 DAVNQSTRLLQSAKt
K346 TRLLQSAKtILRGtE
T347-p RLLQSAKtILRGtEE
T352-p AKtILRGtEEKCGsP
S358-p GtEEKCGsPRIRTLs
T363 CGsPRIRTLsSsRPP
S365-p sPRIRTLsSsRPPLL
S367-p RIRTLsSsRPPLLLR
S376-p PPLLLRLsENsGDEN
S379-p LLRLsENsGDENMsD
S385-p NsGDENMsDVTFDsL
S391-p MsDVTFDsLPssPss
S394-p VTFDsLPssPssAtP
S395-p TFDsLPssPssAtPH
S397-p DsLPssPssAtPHsQ
S398-p sLPssPssAtPHsQK
T400-p PssPssAtPHsQKLD
S403-p PssAtPHsQKLDHLH
G415 HLHWPVFGDLDNMGP
S440 DSENSGDSGYPsEKR
Y442 ENSGDSGYPsEKRSD
S444-p SGDSGYPsEKRSDLD
S469-p NGNRRHEsDEDsLGs
S473-p RHEsDEDsLGsSGRV
S476-p sDEDsLGsSGRVCVE
T484 SGRVCVETWNLLNPS
P490 ETWNLLNPSRLHLPR
S499-p RLHLPRPsAVALEVQ
R515 LNALDLGRKIGKSVL
K516 NALDLGRKIGKSVLG
Y599 NKTKGFDYLLKAAEA
K602 KGFDYLLKAAEAGDR
S626-p FDTGLNLsPDRCQDW
K683 TGGFGLDKNPQRSGD
  rat

 
S18-p EGVDGGGsSGAGRHG
S27 GAGRHGDSDTDSDDD
T29 GRHGDSDTDSDDDEG
S31 HGDSDTDSDDDEGYF
Y37 DSDDDEGYFICPITD
Y59 VNSKGQGYYNNLLKT
Y60 NSKGQGYYNNLLKTE
N61 SKGQGYYNNLLKTEC
K65 GYYNNLLKTECGSTG
T66 YYNNLLKTECGSTGS
G69 NLLKTECGSTGSPAS
S70 LLKTECGSTGSPASS
T71 LKTECGSTGSPASSF
S73 TECGSTGSPASSFHF
S76 GSTGSPASSFHFKEA
S77 STGSPASSFHFKEAW
S134 EVLIKMASQPFGRGA
K161 FLHAQHWKGASNYVA
Y235 EHYIEGKYIKYNSNS
K237 YIEGKYIKYNSNSGF
T339 QDAVNQSTKLLQSAK
K340 DAVNQSTKLLQSAKT
K346 TKLLQSAKTILRGTE
T347 KLLQSAKTILRGTEE
T352 AKTILRGTEEKCGSP
S358 GTEEKCGSPRIRTLs
T363 CGSPRIRTLsGSRPP
S365-p SPRIRTLsGSRPPLL
S367 RIRTLsGSRPPLLLR
S376 PPLLLRLSENSGDEN
S379 LLRLSENSGDENMSD
S385 NSGDENMSDVTFDSL
S391 MSDVTFDSLPSSPSS
S394 VTFDSLPSSPSSATP
S395 TFDSLPSSPSSATPH
S397 DSLPSSPSSATPHSQ
S398 SLPSSPSSATPHSQK
T400 PSSPSSATPHSQKLD
S403 PSSATPHSQKLDHLH
G415 HLHWPVFGDLDNMGP
S440 DSENSGDSGYPsEKR
Y442 ENSGDSGYPsEKRSD
S444-p SGDSGYPsEKRSDLD
S469-p NGNRRPEsDEDSLGS
S473 RPEsDEDSLGSSGRV
S476 sDEDSLGSSGRVCVE
T484 SGRVCVETWNLLNPS
P490 ETWNLLNPSRLHLPR
S499-p RLHLPRPsAVALEVQ
R515 LNALDLGRKIGKSVL
K516 NALDLGRKIGKSVLG
Y599 NKTKGFDYLLKAAEA
K602 KGFDYLLKAAEAGDR
S626 FDTGLNLSPDRCQDW
K683 TGGFGLDKNPQRSGD
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