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NHERF-2 (human)

Overview NHERF-2 Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus. Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Found in a complex with EZR, PODXL and SLC9A3R2. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells. Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX and LPAR2. Interacts with MCC and PODXL. Interacts with SGK1 and KCNJ1/ROMK1. Widely expressed. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Adaptor/scaffold Cellular Component: cytoplasm; apical plasma membrane; plasma membrane; endomembrane system; nucleus Molecular Function: protein C-terminus binding; protein binding; channel regulator activity; beta-catenin binding; PDZ domain binding; receptor binding; phosphatase binding Biological Process: protein complex assembly; positive regulation of membrane potential; inner ear development; negative regulation of phosphoinositide 3-kinase cascade Reference #:  Q15599 (UniProtKB) Alt. Names/Synonyms: E3KARP; MGC104639; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 kinase A regulatory protein E3KARP; NHE3RF2; NHERF-2; NHERF2; NHRF2; OCTS2; SIP-1; SIP1; SLC9A3R2; Sodium-hydrogen exchanger regulatory factor 2; sodium/hydrogen exchanger; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2; Solute carrier family 9 isoform A3 regulatory factor 2; SRY-interacting protein 1; TKA-1; Tyrosine kinase activator protein 1 Gene Symbols: SLC9A3R2 Molecular weight: 37,414 Da Basal Isoelectric point: 7.8  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below NHERF-2 2HE4 - A=147-232 (human) 2OCS - A=9-91 (human) 2D11 - E/F/G/H=310-337 (mouse)

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		Q90	TRLLVVDQETDEELR
0	1		T102-p	ELRRRQLtCTEEMAQ
0	1		S130	PDWAHTGSHSSEAGK
0	1		S133	AHTGSHSSEAGKKDV
0	1		K169	GFNLHSDKSRPGQYI
0	1		S183-p	IRSVDPGsPAARSGL
0	1		K237	PETDEHFKRLRVTPT
0	3		S254-p	HVEGPLPsPVTNGTS
0	1		T260	PsPVTNGTSPAQLNG
0	1		S261	sPVTNGTSPAQLNGG
0	1		S269	PAQLNGGSACSSRsD
0	1		S273	NGGSACSSRsDLPGs
0	2		S275-p	GSACSSRsDLPGsDK
0	5		S280-p	SRsDLPGsDKDTEDG
0	1		K282	sDLPGsDKDTEDGSA
0	2		K291-u	TEDGSAWkQDPFQES
0	1		S303-p	QESGLHLsPTAAEAK

	mouse
K90-u	TQLLVVDkETDEELC
T102	ELCRRQLTCTEEMAH
S130-p	PDWACSGsLGsDTGQ
S133-p	ACSGsLGsDTGQKDV
K169-u	GFNLHSDkSRPGQYI
S183	IRSVDPGSPASHSGL
K237-u	PETDEHFkRLRVVPT
S254-p	HVEGPLPsPVTNGts
T260-p	PsPVTNGtsPAQLNG
S261-p	sPVTNGtsPAQLNGG
S269-p	PAQLNGGsVCSsRsD
S273-p	NGGsVCSsRsDLPGs
S275-p	GsVCSsRsDLPGsEk
S280-p	sRsDLPGsEkDNEDG
K282-u	sDLPGsEkDNEDGST
K291-u	NEDGSTWkRDPFQES
S303-p	QESGLHLsPTAAEAK

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