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Protein Page:
NHERF-2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NHERF-2 Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus. Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Found in a complex with EZR, PODXL and SLC9A3R2. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells. Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX and LPAR2. Interacts with MCC and PODXL. Interacts with SGK1 and KCNJ1/ROMK1. Widely expressed. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; endomembrane system; nucleus
Molecular Function: protein C-terminus binding; protein binding; beta-catenin binding; phosphatase binding; receptor binding
Biological Process: protein complex assembly; negative regulation of phosphoinositide 3-kinase cascade
Reference #:  Q15599 (UniProtKB)
Alt. Names/Synonyms: E3KARP; MGC104639; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 kinase A regulatory protein E3KARP; NHE3RF2; NHERF-2; NHERF2; NHRF2; OCTS2; SIP-1; SIP1; SLC9A3R2; Sodium-hydrogen exchanger regulatory factor 2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2; Solute carrier family 9 isoform A3 regulatory factor 2; SRY-interacting protein 1; TKA-1; Tyrosine kinase activator protein 1
Gene Symbols: SLC9A3R2
Molecular weight: 37,414 Da
Basal Isoelectric point: 7.8  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NHERF-2

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 3 S43-p IRRVEPGsPAEAAAL
0 1 Q90 TRLLVVDQETDEELR
0 1 T102-p ELRRRQLtCTEEMAQ
0 1 S130 PDWAHTGSHsSEAGK
0 1 S132-p WAHTGSHsSEAGKKD
0 1 S133 AHTGSHsSEAGKKDV
0 1 - gap
0 1 S167-p GYGFNLHsDKSRPGQ
0 1 K169 GFNLHsDKSRPGQYI
0 1 S178-p RPGQYIRsVDPGsPA
0 2 S183-p IRsVDPGsPAARSGL
0 1 A186 VDPGsPAARSGLRAQ
0 1 K237 PETDEHFKRLRVtPT
0 1 T242-p HFKRLRVtPTEEHVE
0 6 S254-p HVEGPLPsPVtNGts
0 1 T257-p GPLPsPVtNGtsPAQ
0 2 T260-p PsPVtNGtsPAQLNG
0 3 S261-p sPVtNGtsPAQLNGG
0 1 S269 PAQLNGGSACSSRsD
0 1 S273 NGGSACSSRsDLPGs
0 2 S275-p GSACSSRsDLPGsDK
0 9 S280-p SRsDLPGsDKDTEDG
0 1 K282 sDLPGsDKDTEDGSA
0 2 K291-ub TEDGSAWkQDPFQEs
0 1 S298-p kQDPFQEsGLHLsPT
0 4 S303-p QEsGLHLsPTAAEAK
  NHERF-2 iso2  
S43 IRRVEPGSPAEAAAL
Q90 TRLLVVDQETDEELR
T102 ELRRRQLTCTEEMAQ
S130 PDWAHTGSHSSEAGK
S132 WAHTGSHSSEAGKKD
S133 AHTGSHSSEAGKKDV
- gap
S167 GYGFNLHSDKSRPGQ
K169 GFNLHSDKSRPGQYI
S178 RPGQYIRSVDPGSPA
S183 IRSVDPGSPAARSGL
A186 VDPGSPAARSGLRAQ
K237 PETDEHFKRLRVTPT
T242 HFKRLRVTPTEEHVE
S254 HVEGPLPSPVTNGTS
T257 GPLPSPVTNGTSPAQ
T260 PSPVTNGTSPAQLNG
S261 SPVTNGTSPAQLNGG
S269 PAQLNGGSACSSRSD
S273 NGGSACSSRSDLPGs
S275 GSACSSRSDLPGsDK
S280-p SRSDLPGsDKDTEES
K282 SDLPGsDKDTEESGL
- gap
S287 sDKDTEESGLHLsPT
S292-p EESGLHLsPTAAEAK
  NHERF-2 iso3  
- gap
- gap
- gap
- gap
- gap
- gap
S20-p APGAPPRsPPQRLVQ
S56 GYGFNLHSDKSRPGQ
K58 GFNLHSDKSRPGQYI
S67 RPGQYIRSVDPGSPA
S72 IRSVDPGSPAARSGL
A75 VDPGSPAARSGLRAQ
K126 PETDEHFKRLRVTPT
T131 HFKRLRVTPTEEHVE
S143 HVEGPLPSPVTNGTS
T146 GPLPSPVTNGTSPAQ
T149 PSPVTNGTSPAQLNG
S150 SPVTNGTSPAQLNGG
S158 PAQLNGGSACSSRSD
S162 NGGSACSSRSDLPGS
S164 GSACSSRSDLPGSDK
S169 SRSDLPGSDKDTEDG
K171 SDLPGSDKDTEDGSA
- gap
S187 KQDPFQESGLHLSPT
S192 QESGLHLSPTAAEAK
  mouse

 
S43 IRRVEPGSPAEAAAL
K90-ub TQLLVVDkETDEELC
T102 ELCRRQLTCTEEMAH
S130-p PDWACSGsLGsDTGQ
G132 WACSGsLGsDTGQKD
S133-p ACSGsLGsDTGQKDV
- gap
S167 GYGFNLHSDkSRPGQ
K169-ub GFNLHSDkSRPGQYI
S178 RPGQYIRSVDPGSPA
S183 IRSVDPGSPASHSGL
S186 VDPGSPASHSGLRAQ
K237-ub PETDEHFkRLRVVPT
V242 HFkRLRVVPTEEHVE
S254-p HVEGPLPsPVTNGts
T257 GPLPsPVTNGtsPAQ
T260-p PsPVTNGtsPAQLNG
S261-p sPVTNGtsPAQLNGG
S269-p PAQLNGGsVCSsRsD
S273-p NGGsVCSsRsDLPGs
S275-p GsVCSsRsDLPGsEk
S280-p sRsDLPGsEkDNEDG
K282-ub sDLPGsEkDNEDGST
K291-ub NEDGSTWkRDPFQES
S298 kRDPFQESGLHLsPT
S303-p QESGLHLsPTAAEAK
  rat

 
S43 IRRVEPGSPAEAAAL
K90 TQLLVVDKETDEELC
T102 ELCRRQLTCTEEMAH
S130 PDWACSGSLGSDTGH
G132 WACSGSLGSDTGHKD
S133 ACSGSLGSDTGHKDV
- gap
S167 GYGFNLHSDKSRPGQ
K169 GFNLHSDKSRPGQYI
S178 RPGQYIRSVDPGSPA
S183 IRSVDPGSPAsLSGL
S186-p VDPGSPAsLSGLRAQ
K237 PETDEHFKRLRVVPT
V242 HFKRLRVVPTEDHVE
S254-p HVEGPLPsPVTNGTS
T257 GPLPsPVTNGTSLAQ
T260 PsPVTNGTSLAQLNG
S261 sPVTNGTSLAQLNGG
S269 LAQLNGGSVCSSRSD
S273 NGGSVCSSRSDLPGs
S275 GSVCSSRSDLPGsEK
S280-p SRSDLPGsEKDNEDG
K282 SDLPGsEKDNEDGSA
K291 NEDGSAWKRDPFQES
S298 KRDPFQESGLHLsPT
S303-p QESGLHLsPTAAEAK
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