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DAPK1 (human)

Overview DAPK1 a CAMK protein kinase of the Death-associated protein kinase (DAPK) family. Mediates autophagy and is a positive mediator of ER stress-induced and gamma-interferon induced programmed cell death. Catalytic activity is increased by ER stress. Inhibited by autophosphorylation of S308. DAPK -/- cells are protected from ER stress-induced cell death, and both apoptosis and autophagy are decreased. Colocalizes with the actin filament system. It is a tumor suppressor candidate, activating p53-dependent apoptosis. Expression is reduced in some cancers, and the promoter is frequently hypermethylated in invasive cancers. Increased expression and mislocalization is seen in epilepsy, where it may regulate neuronal death. A structurally unique serine-threonine kinase that carries eight ankyrin repeats and two putative P-loop consensus sites. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Autophagy; Protein kinase, CAMK; EC 2.7.11.1; Kinase, protein; Apoptosis; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family Cellular Component: cytoplasm; actin cytoskeleton Molecular Function: calmodulin binding; protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity Biological Process: regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; induction of apoptosis; protein kinase cascade; regulation of autophagy; protein amino acid phosphorylation Reference #:  P53355 (UniProtKB) Alt. Names/Synonyms: DAP kinase 1; DAPK; DAPK1; Death-associated protein kinase 1; DKFZp781I035 Gene Symbols: DAPK1 Molecular weight: 160,046 Da Basal Isoelectric point: 6.37  Predict pI for various phosphorylation states CST Pathways:  Death Receptor Signaling  |  MAPK/Erk in Growth and Differentiation Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below DAPK1 1IG1 - A=2-285 (human) 1JKK - A=2-285 (human) 1JKL - A=2-285 (human) 1JKS - A=2-285 (human) 1JKT - A/B=2-285 (human) 1P4F - A=2-285 (human) 1WVW - A=1-278 (human) 1WVX - A=1-278 (human) 1WVY - A=1-278 (human) 1YR5 - B=302-320 (human) 2W4J - A=1-277 (human) 2W4K - A=1-302 (human) 2X0G - A=1-334 (human) 2XUU - A=1-334 (human) 2XZS - A/B=1-312 (human) 2Y0A - A=2-304 (human) 2Y4P - A/D/C/B=1-285 (human) 2Y4V - B=302-320 (human) 2YAK - A=1-285 (human) 3DFC - B=1-295 (human) 3DGK - A=1-295 (human) 3EH9 - A=2-285 (human) 3EHA - A=2-285 (human) 3F5G - A=2-285 (human) 3F5U - A=1-285 (human) 3GU4 - A=1-285 (human) 3GU5 - A=1-285 (human) 3GU6 - A=1-285 (human) 3GU7 - A=1-285 (human) 3GU8 - A=1-285 (human) 3GUB - A=1-285 (human) 3ZXT - A/D/C/B=1-285 (human)

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1IG1 1JKK 1JKL 1JKS 1JKT 1P4F 1WVW 1WVX 1WVY 1YR5 2W4J 2W4K 2X0G 2XUU 2XZS 2Y0A 2Y4P 2Y4V 2YAK 3DFC 3DGK 3EH9 3EHA 3F5G 3F5U 3GU4 3GU5 3GU6 3GU7 3GU8 3GUB 3ZXT  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
apoptosis, altered: S289‑p, S308‑p, S734‑p cell adhesion, altered: S308‑p cell growth, altered: S308‑p enzymatic activity, induced: S734‑p enzymatic activity, inhibited: S308‑p inhibition: S289‑p molecular association, regulation: S308‑p protein degradation: S308‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		Y12-p	RQENVDDyyDTGEEL
0	1		Y13-p	QENVDDyyDTGEELG
0	1		Y39-p	EKSTGLQyAAKFIKK
0	1		S57-p	KSSRRGVsREDIERE
1	25		S289-p	QALSRKAsAVNMEkF
0	3		K295-a	AsAVNMEkFkKFAAR
0	3		K297-a	AVNMEkFkKFAARKK
7	0		S308-p	ARKKWKQsVRLISLC
0	1		S319	ISLCQRLSRSFLSRs
0	2		S326-p	SRSFLSRsNMSVARs
0	4		S333-p	sNMSVARsDDTLDEE
0	1		T336	SVARsDDTLDEEDSF
1	0		Y490-p	HCAAWHGyySVAKAL
1	0		Y491-p	CAAWHGyySVAKALC
0	1		S606-p	ANCNLDIsNKYGRTP
0	1		K708	TTLVESLKCGLLRSF
0	1		S724-p	RRRRPRLssTNsSRF
0	1		S725-p	RRRPRLssTNsSRFP
0	1		S728-p	PRLssTNsSRFPPsP
1	6		S734-p	NsSRFPPsPLASKPT
0	1		K875	EPIAFGGKLKNPLQV
0	1		K912	DKDTSLLKEIRNRFG
0	1		K939	DAGASGSKDMKVLRN
0	1		K942	ASGSKDMKVLRNHLQ
0	1		Y1060-p	TPRALHHyRGRYTVE
0	1		K1108	VDVPALIKTDNLHRS
0	2		S1115	KTDNLHRSWADEEDE
0	1		T1266-p	RAQTLKEtsLtNTMG
0	1		S1267-p	AQTLKEtsLtNTMGG
0	1		T1269-p	TLKEtsLtNTMGGYK
0	2		-	gap

	mouse
Y12	RQENVDDYYDTGEEL
Y13	QENVDDYYDTGEELG
Y39	EKSTGLQYAAKFIKK
S57	KSSRRGVSREDIERE
S289-p	QALSRKAsAVNMEKF
K295	AsAVNMEKFKKFAAR
K297	AVNMEKFKKFAARKK
S308-p	ARKKWKQsVRLISLC
S319-p	ISLCQRLsRSFLSRs
S326-p	sRSFLSRsNMSVARs
S333-p	sNMSVARsDDtLDEE
T336-p	SVARsDDtLDEEDSF
Y490	HCAAWHGYYSVAKAL
Y491	CAAWHGYYSVAKALC
S606	ASCNLDISNKYGRTP
K708-u	STLVESLkCGLLRSF
S724	RRRRPRLSSTNSTRF
S725	RRRPRLSSTNSTRFP
S728	PRLSSTNSTRFPPsP
S734-p	NSTRFPPsPLAAKPT
K875-u	EPIAFGGkLKNPLRV
K912-u	DKDTSLLkEIRNRFG
K939-u	DAGASGSkDIkVLRN
K942-u	ASGSkDIkVLRNHLQ
Y1060	TPRALHHYRGRYTME
K1108-u	VDIPALIkTDSLQRs
S1115-p	kTDSLQRsWADEEDE
S1266	RAQTLKESSLTNTMG
S1267	AQTLKESSLTNTMGG
T1269	TLKESSLTNTMGGYK
S1433-p	SVVSRRDsHAWTPLY

	rat
Y12	RQENVDDYYDTGEEL
Y13	QENVDDYYDTGEELG
Y39	EKSTGLQYAAKFIKK
S57	KSSRRGVSREDIERE
S289	QALSRKASAVNMEKF
K295	ASAVNMEKFKKFAAR
K297	AVNMEKFKKFAARKK
S308	ARKKWKQSVRLISLC
S319	ISLCQRLSRSFLSRS
S326	SRSFLSRSNMSVARS
S333	SNMSVARSDDTLDEE
T336	SVARSDDTLDEEDSF
Y490	HCAAWHGYYSVARAL
Y491	CAAWHGYYSVARALC
S606	ASCNLDISNKYGRTP
K708	STLVESLKCGLLRSF
S724	RRRRPRLSSTNSTRF
S725	RRRPRLSSTNSTRFP
S728	PRLSSTNSTRFPPsP
S734-p	NSTRFPPsPLATKPT
K875	EPIAFGGKLKNPLRV
K912	DKDTSLLKEIRNRFG
K939	DAGASGSKDIKVLRN
K942	ASGSKDIKVLRNHLQ
Y1060	TPRALHHYRGRYTME
K1108	VDIPALIKTDSLQRS
S1115	KTDSLQRSWADEEDE
S1266	RAQTLKESSLTNTMG
S1267	AQTLKESSLTNTMGG
T1269	TLKESSLTNTMGGYK
-	gap

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