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Protein Page:
DAPK1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
DAPK1 a CAMK protein kinase of the Death-associated protein kinase (DAPK) family. Mediates autophagy and is a positive mediator of ER stress-induced and gamma-interferon induced programmed cell death. Catalytic activity is increased by ER stress. Inhibited by autophosphorylation of S308. DAPK -/- cells are protected from ER stress-induced cell death, and both apoptosis and autophagy are decreased. Colocalizes with the actin filament system. It is a tumor suppressor candidate, activating p53-dependent apoptosis. Expression is reduced in some cancers, and the promoter is frequently hypermethylated in invasive cancers. Increased expression and mislocalization is seen in epilepsy, where it may regulate neuronal death. A structurally unique serine-threonine kinase that carries eight ankyrin repeats and two putative P-loop consensus sites. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Autophagy; Protein kinase, CAMK; Apoptosis; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family
Cellular Component: cytoplasm; actin cytoskeleton
Molecular Function: calmodulin binding; protein serine/threonine kinase activity; identical protein binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding; protein kinase activity
Biological Process: regulation of apoptosis; induction of apoptosis via death domain receptors; positive regulation of apoptosis; apoptosis; negative regulation of translation; protein amino acid autophosphorylation; regulation of autophagy; protein amino acid phosphorylation; negative regulation of apoptosis
Reference #:  P53355 (UniProtKB)
Alt. Names/Synonyms: DAP kinase 1; DAPK; DAPK1; Death-associated protein kinase 1; DKFZp781I035
Gene Symbols: DAPK1
Molecular weight: 160,046 Da
Basal Isoelectric point: 6.37  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling  |  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

DAPK1

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S289‑p, S308‑p, S734‑p
cell adhesion, altered: S308‑p
cell growth, altered: S308‑p
activity, inhibited: S289‑p
enzymatic activity, induced: S734‑p
enzymatic activity, inhibited: S308‑p
molecular association, regulation: S308‑p
protein degradation: S308‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y12-p RQENVDDyyDTGEEL
0 1 Y13-p QENVDDyyDTGEELG
1 25 S289-p QALSRKAsAVNMEkF
0 3 K295-a AsAVNMEkFkKFAAR
0 3 K297-a AVNMEkFkKFAARKK
7 0 S308-p ARKKWKQsVRLISLC
0 2 S319-p ISLCQRLsRSFLSRs
0 2 S326-p sRSFLSRsNMSVARs
0 5 S333-p sNMSVARsDDTLDEE
0 1 T336 SVARsDDTLDEEDSF
1 0 Y490-p HCAAWHGyySVAKAL
1 0 Y491-p CAAWHGyySVAKALC
0 1 K708 TTLVESLKCGLLRSF
1 6 S734-p NSSRFPPsPLASKPT
0 1 K875 EPIAFGGKLKNPLQV
0 1 K912 DKDTSLLKEIRNRFG
0 1 K939 DAGASGSKDMKVLRN
0 1 K942 ASGSKDMKVLRNHLQ
0 1 K1108 VDVPALIKTDNLHRS
0 2 S1115 KTDNLHRSWADEEDE
0 2 - gap
  mouse

 
Y12 RQENVDDYYDTGEEL
Y13 QENVDDYYDTGEELG
S289-p QALSRKAsAVNMEKF
K295 AsAVNMEKFKKFAAR
K297 AVNMEKFKKFAARKK
S308-p ARKKWKQsVRLISLC
S319-p ISLCQRLsRSFLSRs
S326-p sRSFLSRsNMSVARs
S333-p sNMSVARsDDtLDEE
T336-p SVARsDDtLDEEDSF
Y490 HCAAWHGYYSVAKAL
Y491 CAAWHGYYSVAKALC
K708-u STLVESLkCGLLRSF
S734-p NSTRFPPsPLAAKPT
K875-u EPIAFGGkLKNPLRV
K912-u DKDTSLLkEIRNRFG
K939-u DAGASGSkDIkVLRN
K942-u ASGSkDIkVLRNHLQ
K1108-u VDIPALIkTDSLQRs
S1115-p kTDSLQRsWADEEDE
S1433-p SVVSRRDsHAWTPLY
  rat

 
Y12 RQENVDDYYDTGEEL
Y13 QENVDDYYDTGEELG
S289 QALSRKASAVNMEKF
K295 ASAVNMEKFKKFAAR
K297 AVNMEKFKKFAARKK
S308 ARKKWKQSVRLISLC
S319 ISLCQRLSRSFLSRS
S326 SRSFLSRSNMSVARS
S333 SNMSVARSDDTLDEE
T336 SVARSDDTLDEEDSF
Y490 HCAAWHGYYSVARAL
Y491 CAAWHGYYSVARALC
K708 STLVESLKCGLLRSF
S734-p NSTRFPPsPLATKPT
K875 EPIAFGGKLKNPLRV
K912 DKDTSLLKEIRNRFG
K939 DAGASGSKDIKVLRN
K942 ASGSKDIKVLRNHLQ
K1108 VDIPALIKTDSLQRS
S1115 KTDSLQRSWADEEDE
- gap
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