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Protein Page:
Kit (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Kit a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; PDGFR family
Cellular Component: extracellular space; internal side of plasma membrane; mast cell granule; acrosome; plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; protein homodimerization activity; protease binding; protein-tyrosine kinase activity; cytokine binding; metal ion binding; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; ATP binding
Biological Process: nerve growth factor receptor signaling pathway; somatic stem cell maintenance; activation of MAPK activity; germ cell programmed cell death; positive regulation of JAK-STAT cascade; lymphoid progenitor cell differentiation; positive regulation of long-term neuronal synaptic plasticity; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell shape; epithelial cell proliferation; germ cell migration; erythrocyte differentiation; somatic stem cell division; T cell differentiation; fibroblast growth factor receptor signaling pathway; mast cell chemotaxis; embryonic hemopoiesis; stem cell differentiation; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of tyrosine phosphorylation of Stat1 protein; immature B cell differentiation; glycosphingolipid metabolic process; positive regulation of transcription factor activity; regulation of pigmentation during development; mast cell cytokine production; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; signal transduction; ovarian follicle development; positive regulation of MAPKKK cascade; myeloid progenitor cell differentiation; melanocyte differentiation; positive regulation of cell proliferation; negative regulation of programmed cell death; visual learning; hemopoiesis; inflammatory response; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; phosphoinositide-mediated signaling; dendritic cell cytokine production; cytokine and chemokine mediated signaling pathway; stem cell maintenance; male gonad development; positive regulation of phosphoinositide 3-kinase activity; mast cell degranulation; regulation of cell proliferation; positive regulation of pseudopodium formation; pigmentation; positive regulation of tyrosine phosphorylation of Stat5 protein; gut development; actin cytoskeleton reorganization; innate immune response; spermatogenesis; spermatid development; positive regulation of cell migration
Reference #:  P10721 (UniProtKB)
Alt. Names/Synonyms: C-Kit; CD117; KIT; Mast/stem cell growth factor receptor; PBT; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Gene Symbols: KIT
Molecular weight: 109,865 Da
Basal Isoelectric point: 6.54  Predict pI for various phosphorylation states
CST Pathways:  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Kit

Protein Structure Not Found.


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Sites Implicated In
cell adhesion, altered: Y721‑p
cell growth, altered: Y568‑p, Y570‑p
activity, induced: Y568‑p, Y570‑p, Y721‑p
enzymatic activity, induced: Y547‑p, Y553‑p, Y568‑p, Y570‑p
enzymatic activity, inhibited: S741‑p, S746‑p
molecular association, regulation: Y568‑p, Y570‑p, Y900‑p, Y936‑p
phosphorylation: Y568‑p, S741‑p, S746‑p
protein degradation: Y568‑p, Y936‑p
protein stabilization: Y900‑p
receptor internalization, altered: Y568‑p, Y936‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S215-p FKAVPVVsVsKASYL
0 1 S217-p AVPVVsVsKASYLLR
0 1 T232-p EGEEFTVtCTIKDVS
0 1 S240-p CTIKDVSssVYSTWK
0 1 S241-p TIKDVSssVYSTWKR
0 1 T274 FNYERQATLTISSAR
0 1 S279 QATLTISSARVNDSG
0 1 T354-p QWIYMNRtFTDKWED
0 1 Y362-p FTDKWEDyPKSENES
1 0 Y547-p VMILTYKyLQKPMyE
1 0 Y553-p KyLQKPMyEVQWKVV
12 14 Y568-p EEINGNNyVyIDPTQ
8 10 Y570-p INGNNyVyIDPTQLP
0 2 Y578-p IDPTQLPyDHKWEFP
0 1 K593 RNRLSFGKTLGAGAF
0 1 K602 LGAGAFGKVVEATAy
0 8 Y609-p KVVEATAyGLIKSDA
0 4 K693 RDSFICSKQEDHAEA
5 24 Y703-p DHAEAALyKNLLHSK
0 1 S713 LLHSKESSCSDStNE
0 3 T718-p ESSCSDStNEyMDMK
14 52 Y721-p CSDStNEyMDMKPGV
0 3 S729-p MDMKPGVsyVVPTkA
2 27 Y730-p DMKPGVsyVVPTkAD
0 1 K735-ub VsyVVPTkADkRRsV
0 1 K738-ub VVPTkADkRRsVRIG
1 0 S741-p TkADkRRsVRIGsyI
2 2 S746-p RRsVRIGsyIERDVT
0 12 Y747-p RsVRIGsyIERDVTP
0 2 T801-p AARNILLtHGRITKI
1 1 S821-p ARDIKNDsNyVVKGN
3 9 Y823-p DIKNDsNyVVKGNAR
0 1 K884 SKFYKMIKEGFRMLs
1 0 S891-p KEGFRMLsPEHAPAE
1 2 Y900-p EHAPAEMyDIMKTCW
0 1 K904 AEMyDIMKTCWDADP
0 1 K926 QIVQLIEKQISEsTN
0 2 S931-p IEKQISEsTNHIysN
4 102 Y936-p SEsTNHIysNLANCs
0 3 S937-p EsTNHIysNLANCsP
0 13 S943-p ysNLANCsPNRQKPV
1 1 S959-p DHSVRINsVGSTASS
3073 : Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb
3391 : Phospho-c-Kit (Tyr719) Antibody
  mouse

 
S216 IKAIPVVSVPETSHL
P218 AIPVVSVPETSHLLK
V233 KGDTFTVVCTIKDVS
T241 CTIKDVSTSVNSMWL
S242 TIKDVSTSVNSMWLK
T277-p FNYERQEtLTISsAR
S282-p QEtLTISsARVDDSG
T357 QWIYMNRTSANKGKD
Y365 SANKGKDYVKSDNKS
Y550 VMVLTYKYLQKPMYE
Y556 KYLQKPMYEVQWKVV
Y571-p EEINGNNyVyIDPTQ
Y573-p INGNNyVyIDPTQLP
Y581-p IDPTQLPyDHKWEFP
K596-ub RNRLSFGkTLGAGAF
K605-ub LGAGAFGkVVEATAy
Y612-p kVVEATAyGLIKSDA
K696-ub RDSFIFSkQEEQAEA
Y706-p EQAEAALyKNLLHST
S716-p LLHSTEPsCDSsNEy
S720-p TEPsCDSsNEyMDMK
Y723-p sCDSsNEyMDMKPGV
S731-p MDMKPGVsyVVPTKT
Y732-p DMKPGVsyVVPTKTD
K737 VsyVVPTKTDKRRSA
K740 VVPTKTDKRRSARID
S743 TKTDKRRSARIDsyI
S748-p RRSARIDsyIERDVT
Y749-p RSARIDsyIERDVTP
T803 AARNILLTHGRITKI
S823 ARDIRNDSNyVVKGN
Y825-p DIRNDSNyVVKGNAR
K886-ac SKFYKMIkEGFRMVS
S893 kEGFRMVSPEHAPAE
Y902-p EHAPAEMyDVMkTCW
K906-ac AEMyDVMkTCWDADP
K928-ub QVVQLIEkQISDSTK
S933 IEkQISDSTKHIysN
Y938-p SDSTKHIysNLANCN
S939-p DSTKHIysNLANCNP
N945 ysNLANCNPNPENPV
S962 DHSVRVNSVGSSASS
3391 : Phospho-c-Kit (Tyr719) Antibody
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