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Chk1 (human)

Overview Chk1 a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01. Note: This description may include information from UniProtKB. Protein type: EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; CHK1 subfamily Cellular Component: nucleoplasm; centrosome; chromosome, telomeric region; condensed nuclear chromosome; replication fork; cytosol; chromatin; nucleus Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding Biological Process: DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis; DNA damage checkpoint; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; DNA replication; cell cycle checkpoint; response to DNA damage stimulus Reference #:  O14757 (UniProtKB) Alt. Names/Synonyms: Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1; CHK1 checkpoint homolog (S. pombe); Serine/threonine-protein kinase Chk1 Gene Symbols: CHEK1 Molecular weight: 54,434 Da Basal Isoelectric point: 8.5  Predict pI for various phosphorylation states CST Pathways:  Apoptosis  |  Cell Cycle: G1/S Checkpoint  |  Cell Cycle: G2/M DNA Damage Checkpoint Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Chk1 1IA8 - A=1-289 (human) 1NVQ - A=1-289 (human) 1NVR - A=1-289 (human) 1NVS - A=1-289 (human) 1ZLT - A=1-293 (human) 1ZYS - A=1-273 (human) 2AYP - A=1-269 (human) 2BR1 - A=1-289 (human) 2BRB - A=1-289 (human) 2BRG - A=1-289 (human) 2BRH - A=1-289 (human) 2BRM - A=1-289 (human) 2BRN - A=1-289 (human) 2BRO - A=1-289 (human) 2C3J - A=1-289 (human) 2C3K - A=1-289 (human) 2C3L - A=1-289 (human) 2CGU - A=1-289 (human) 2CGV - A=1-289 (human) 2CGW - A=1-289 (human) 2CGX - A=1-289 (human) 2E9N - A=2-270 (human) 2E9O - A=2-270 (human) 2E9P - A=2-270 (human) 2E9U - A=2-270 (human) 2E9V - A/B=2-269 (human) 2GDO - A=1-293 (human) 2GHG - A=2-270 (human) 2HOG - A=2-307 (human) 2HXL - A=2-307 (human) 2HXQ - A=2-307 (human) 2HY0 - A=2-307 (human) 2QHM - A=1-307 (human) 2QHN - A=1-307 (human) 2R0U - A=1-307 (human) 2WMQ - A=1-289 (human) 2WMR - A=1-289 (human) 2WMS - A=1-289 (human) 2WMT - A=1-289 (human) 2WMU - A=1-289 (human) 2WMV - A=1-289 (human) 2WMW - A=1-289 (human) 2WMX - A=1-289 (human) 2X8D - A=1-289 (human) 2X8E - A=1-276 (human) 2X8I - A=1-289 (human) 2XEY - A=1-289 (human) 2XEZ - A=1-289 (human) 2XF0 - A=1-289 (human) 2YDI - A=1-289 (human) 2YDJ - A/B=1-276 (human) 2YDK - A=1-276 (human) 2YER - A=1-276 (human) 2YEX - A=1-276 (human) 2YM3 - A=1-289 (human) 2YM4 - A=1-289 (human) 2YM5 - A=1-289 (human) 2YM6 - A=1-289 (human) 2YM7 - A=1-289 (human) 2YM8 - A=1-289 (human) 2YWP - A=2-270 (human) 3F9N - A=2-307 (human) 3JVR - A=2-272 (human) 3JVS - A=2-272 (human) 3NLB - A=1-289 (human) 3OT3 - A=2-274 (human) 3OT8 - A=2-274 (human) 3PA3 - A=2-274 (human) 3PA4 - A=2-274 (human) 3PA5 - A=2-274 (human) 3TKH - A=1-307 (human) 3TKI - A=1-307 (human) 3U9N - A=2-274 (human) 4FSM - A=2-280 (human) 4FSN - A=4-282 (human) 4FSQ - A=2-280 (human) 4FSR - A=2-280 (human) 4FST - A=2-270 (human) 4FSU - A=2-280 (human) 4FSW - A=2-280 (human) 4FSY - A=2-280 (human) 4FSZ - A=2-280 (human) 4FT0 - A=2-280 (human) 4FT3 - A=2-280 (human) 4FT5 - A=2-280 (human) 4FT7 - A=2-280 (human) 4FT9 - A=2-280 (human) 4FTA - A=2-280 (human) 4FTC - A=2-280 (human) 4FTI - A=2-280 (human) 4FTJ - A=2-280 (human) 4FTK - A=2-280 (human) 4FTL - A=2-280 (human) 4FTM - A=2-280 (human) 4FTN - A=2-280 (human) 4FTO - A=2-280 (human) 4FTQ - A=2-280 (human) 4FTR - A=2-280 (human) 4FTT - A=2-280 (human) 4FTU - A=2-280 (human) 4GH2 - A=2-280 (human) 4HYH - A=1-289 (human) 4HYI - A=1-289 (human) 4JIK - A=2-274 (human) Substrate Sequence Logo

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1IA8 1NVQ 1NVR 1NVS 1ZLT 1ZYS 2AYP 2BR1 2BRB 2BRG 2BRH 2BRM 2BRN 2BRO 2C3J 2C3K 2C3L 2CGU 2CGV 2CGW 2CGX 2E9N 2E9O 2E9P 2E9U 2E9V 2GDO 2GHG 2HOG 2HXL 2HXQ 2HY0 2QHM 2QHN 2R0U 2WMQ 2WMR 2WMS 2WMT 2WMU 2WMV 2WMW 2WMX 2X8D 2X8E 2X8I 2XEY 2XEZ 2XF0 2YDI 2YDJ 2YDK 2YER 2YEX 2YM3 2YM4 2YM5 2YM6 2YM7 2YM8 2YWP 3F9N 3JVR 3JVS 3NLB 3OT3 3OT8 3PA3 3PA4 3PA5 3TKH 3TKI 3U9N 4FSM 4FSN 4FSQ 4FSR 4FST 4FSU 4FSW 4FSY 4FSZ 4FT0 4FT3 4FT5 4FT7 4FT9 4FTA 4FTC 4FTI 4FTJ 4FTK 4FTL 4FTM 4FTN 4FTO 4FTQ 4FTR 4FTT 4FTU 4GH2 4HYH 4HYI 4JIK  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
apoptosis, altered: S345‑p cell cycle regulation: S280‑p, S286‑p, S296‑p, S301‑p, S317‑p, S345‑p cell growth, altered: S296‑p enzymatic activity, induced: S296‑p, S317‑p, S345‑p enzymatic activity, inhibited: S280‑p intracellular localization: S280‑p, S286‑p, S296‑p, S301‑p, S345‑p molecular association, regulation: S280‑p, S296‑p, S317‑p, S345‑p protein degradation: S280‑p, S345‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		K145-u	LDERDNLkISDFGLA
0	12		T279-p	GAKRPRVtsGGVsEs
6	139		S280-p	AKRPRVtsGGVsEsP
0	10		S284-p	RVtsGGVsEsPsGFs
5	16		S286-p	tsGGVsEsPsGFskH
0	6		S288-p	GGVsEsPsGFskHIQ
0	2		S291-p	sEsPsGFskHIQsNL
0	1		K292-u	EsPsGFskHIQsNLD
9	9		S296-p	GFskHIQsNLDFsPV
5	6		S301-p	IQsNLDFsPVNsASs
0	4		S305-p	LDFsPVNsASsEENV
0	4		S308-p	sPVNsASsEENVkYs
0	1		K313-u	ASsEENVkYsssQPE
0	1		S315-p	sEENVkYsssQPEPR
0	1		S316-p	EENVkYsssQPEPRT
51	10		S317-p	ENVkYsssQPEPRTG
0	1		T330-p	TGLSLWDtsPsYIDK
0	2		S331-p	GLSLWDtsPsYIDKL
0	2		S333-p	SLWDtsPsYIDKLVQ
0	3		S343-p	DKLVQGIsFsQPtCP
93	11		S345-p	LVQGIsFsQPtCPDH
0	5		T348-p	GIsFsQPtCPDHMLL
0	7		S357-p	PDHMLLNsQLLGtPG
0	13		T362-p	LNsQLLGtPGssQNP
0	2		S365-p	QLLGtPGssQNPWQR
0	1		S366-p	LLGtPGssQNPWQRL
0	1		K383-u	RMTRFFTkLDADKsY
0	1		S389-p	TkLDADKsYQCLKET
0	1		K399-u	CLKETCEkLGYQWKK
2	1		K436-u	NLLEMDDkILVDFRL
0	2		K452-a	KGDGLEFkRHFLKIK
0	3		I463	LKIKGKLIDIVssQK
0	1		S467-p	GKLIDIVssQKIWLP
0	2		S468-p	KLIDIVssQKIWLPA

	mouse
K145	LDERDNLKISDFGLA
T279	GAKRPRATsGGMSEs
S280-p	AKRPRATsGGMSEsS
S284	RATsGGMSEsSSGFS
S286-p	TsGGMSEsSSGFSKH
S288	GGMSEsSSGFSKHIH
S291	SEsSSGFSKHIHsNL
K292	EsSSGFSKHIHsNLD
S296-p	GFSKHIHsNLDFsPV
S301-p	IHsNLDFsPVNNGSs
N305	LDFsPVNNGSsEETV
S308-p	sPVNNGSsEETVKFs
K313	GSsEETVKFsSsQPE
S315-p	sEETVKFsSsQPEPR
S316	EETVKFsSsQPEPRT
S317-p	ETVKFsSsQPEPRTG
T330	TGLSLWDTGPSNVDK
G331	GLSLWDTGPSNVDKL
S333	SLWDTGPSNVDKLVQ
S343	DKLVQGISFsQPTCP
S345-p	LVQGISFsQPTCPEH
T348	GISFsQPTCPEHMLV
S357	PEHMLVNSQLLGTPG
T362	VNSQLLGTPGSSQNP
S365	QLLGTPGSSQNPWQR
S366	LLGTPGSSQNPWQRL
K383	RMTRFFTKLDADKSY
S389	TKLDADKSYQCLKET
K399	CLKETFEKLGYQWKK
K436	NLVEMDEKILVDFRL
K452	KGDGLEFKRHFLKIK
S463-p	LKIKGKLsDVVSSQK
S467	GKLsDVVSSQKVWFP
S468	KLsDVVSSQKVWFPV

	rat
K145	LDERDNLKISDFGLA
T279	GAKRPRATSGGMSES
S280	AKRPRATSGGMSESS
S284	RATSGGMSESSSGFS
S286	TSGGMSESSSGFSKH
S288	GGMSESSSGFSKHIH
S291	SESSSGFSKHIHSNL
K292	ESSSGFSKHIHSNLD
S296	GFSKHIHSNLDFSPI
S301	IHSNLDFSPINSGSS
S305	LDFSPINSGSSEENV
S308	SPINSGSSEENVKFS
K313	GSSEENVKFSSSQPE
S315	SEENVKFSSSQPEPR
S316	EENVKFSSSQPEPRT
S317	ENVKFSSSQPEPRTG
T330	TGLSLWDTGPSNVDK
G331	GLSLWDTGPSNVDKL
S333	SLWDTGPSNVDKLVQ
S343	DKLVQGISFSQPTCP
S345	LVQGISFSQPTCPDH
T348	GISFSQPTCPDHMLV
S357	PDHMLVNSQLLGTPG
T362	VNSQLLGTPGSSQNP
S365	QLLGTPGSSQNPWQR
S366	LLGTPGSSQNPWQRL
K383	RMTRFFTKLDADKSY
S389	TKLDADKSYQCLKET
K399	CLKETFEKLGYQWKK
K436	NLVEMDEKILVDFRL
K452	KGDGLEFKRHFLKIK
S463	LKIKGKLSDIVSSQK
S467	GKLSDIVSSQKVWFP
S468	KLSDIVSSQKVWFPV

	chicken
K145	LDERDNLKISDFGLA
S279	GTKRGRVSSGGVTEs
S280	TKRGRVSSGGVTEsP
T284	RVSSGGVTEsPGALP
S286-p	SSGGVTEsPGALPKH
G288	GGVTEsPGALPKHIR
P291	TEsPGALPKHIRSDT
K292	EsPGALPKHIRSDTD
S296	ALPKHIRSDTDFsPV
S301-p	IRSDTDFsPVKSALG
S305	TDFsPVKSALGEDKA
G308	sPVKSALGEDKASYS
S313	ALGEDKASYSTSQPE
S315	GEDKASYSTSQPEPG
T316	EDKASYSTSQPEPGT
S317	DKASYSTSQPEPGTG
S330	TGGALWDSSTGSIDR
S331	GGALWDSSTGSIDRL
G333	ALWDSSTGSIDRLVQ
S343	DRLVQGISFsQPACP
S345-p	LVQGISFsQPACPEH
A348	GISFsQPACPEHMLL
S357	PEHMLLNSQLLGTPG
T362	LNSQLLGTPGSSQSP
S365	QLLGTPGSSQSPWQR
S366	LLGTPGSSQSPWQRL
K383	RMTRFFTKLDADGSY
S389	TKLDADGSYRSLRDV
K399	SLRDVCEKMGYGWKQ
R436	NLLEMESRILVDFRL
K452	KGDGLEFKRHFLKIK
S463	LKIKGKLSDVVSTQK
S467	GKLSDVVSTQKVWLP
T468	KLSDVVSTQKVWLPP

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