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Protein Page:
Chk1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Chk1 a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; CHK1 subfamily
Cellular Component: nucleoplasm; centrosome; extracellular space; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; nucleolus; replication fork; nucleus; cytosol; chromatin
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding
Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; negative regulation of mitosis; DNA damage checkpoint; peptidyl-threonine phosphorylation; DNA repair; DNA replication; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; response to DNA damage stimulus; regulation of cell proliferation
Reference #:  O14757 (UniProtKB)
Alt. Names/Synonyms: Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1; CHK1 checkpoint homolog (S. pombe); Serine/threonine-protein kinase Chk1
Gene Symbols: CHEK1
Molecular weight: 54,434 Da
Basal Isoelectric point: 8.5  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Chk1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S345‑p
cell cycle regulation: S280‑p, S286‑p, S296‑p, S301‑p, S317‑p, S345‑p
cell growth, altered: S296‑p
enzymatic activity, induced: S296‑p, S317‑p, S345‑p
enzymatic activity, inhibited: S280‑p
intracellular localization: S280‑p, S286‑p, S296‑p, S301‑p, S345‑p
molecular association, regulation: S280‑p, S296‑p, S317‑p, S345‑p
protein degradation: S280‑p, S345‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 K132-ub GITHRDIkPENLLLD
0 1 K145-ub LDERDNLkISDFGLA
0 12 T279-p GAKRPRVtsGGVsEs
6 142 S280-p AKRPRVtsGGVsEsP
0 11 S284-p RVtsGGVsEsPsGFs
5 17 S286-p tsGGVsEsPsGFskH
0 6 S288-p GGVsEsPsGFskHIQ
1 3 S291-p sEsPsGFskHIQsNL
0 1 K292-ub EsPsGFskHIQsNLD
10 11 S296-p GFskHIQsNLDFsPV
5 10 S301-p IQsNLDFsPVNsAss
0 4 S305-p LDFsPVNsAssEENV
0 1 S307-p FsPVNsAssEENVkY
0 4 S308-p sPVNsAssEENVkYs
0 1 K313-ub AssEENVkYsssQPE
0 1 S315-p sEENVkYsssQPEPR
1 2 S316-p EENVkYsssQPEPRT
60 10 S317-p ENVkYsssQPEPRTG
0 1 T330-p TGLSLWDtsPsYIDK
0 3 S331-p GLSLWDtsPsYIDKL
0 3 S333-p SLWDtsPsYIDKLVQ
0 3 S343-p DKLVQGIsFsQPtCP
107 11 S345-p LVQGIsFsQPtCPDH
0 5 T348-p GIsFsQPtCPDHMLL
0 7 S357-p PDHMLLNsQLLGtPG
0 13 T362-p LNsQLLGtPGssQNP
0 2 S365-p QLLGtPGssQNPWQR
0 1 S366-p LLGtPGssQNPWQRL
0 1 K383-ub RMTRFFTkLDADKsY
0 1 S389-p TkLDADKsYQCLKET
0 1 K399-ub CLKETCEkLGYQWKK
2 1 K436-ub NLLEMDDkILVDFRL
0 2 K452-ac KGDGLEFkRHFLKIK
0 3 I463 LKIKGKLIDIVssQK
0 2 S467-p GKLIDIVssQKIWLP
0 3 S468-p KLIDIVssQKIWLPA
2347 : Phospho-Chk1(Ser280) Antibody
2349 : Phospho-Chk1 (Ser296) Antibody
2344 : Phospho-Chk1 (Ser317) Antibody
8191 : Phospho-Chk1 (Ser317) (D7H2) Rabbit mAb
12302 : Phospho-Chk1 (Ser317) (D12H3) XP(R) Rabbit mAb
13959 : Phospho-Chk1 (Ser317) (D12H3) XP(R) Rabbit mAb (PE Conjugate)
2341 : Phospho-Chk1 (Ser345) Antibody
2348 : Phospho-Chk1 (Ser345) (133D3) Rabbit mAb
12268 : Phospho-Chk1 (Ser345) (133D3) Rabbit mAb (PE Conjugate)
  mouse

 
K132 GITHRDIKPENLLLD
K145 LDERDNLKISDFGLA
T279 GAKRPRATsGGMSEs
S280-p AKRPRATsGGMSEsS
S284 RATsGGMSEsSSGFS
S286-p TsGGMSEsSSGFSKH
S288 GGMSEsSSGFSKHIH
S291 SEsSSGFSKHIHsNL
K292 EsSSGFSKHIHsNLD
S296-p GFSKHIHsNLDFsPV
S301-p IHsNLDFsPVNNGSs
N305 LDFsPVNNGSsEETV
S307 FsPVNNGSsEETVKF
S308-p sPVNNGSsEETVKFs
K313 GSsEETVKFsSsQPE
S315-p sEETVKFsSsQPEPR
S316 EETVKFsSsQPEPRT
S317-p ETVKFsSsQPEPRTG
T330 TGLSLWDTGPSNVDK
G331 GLSLWDTGPSNVDKL
S333 SLWDTGPSNVDKLVQ
S343 DKLVQGISFsQPTCP
S345-p LVQGISFsQPTCPEH
T348 GISFsQPTCPEHMLV
S357 PEHMLVNSQLLGTPG
T362 VNSQLLGTPGSSQNP
S365 QLLGTPGSSQNPWQR
S366 LLGTPGSSQNPWQRL
K383 RMTRFFTKLDADKSY
S389 TKLDADKSYQCLKET
K399 CLKETFEKLGYQWKK
K436 NLVEMDEKILVDFRL
K452 KGDGLEFKRHFLKIK
S463-p LKIKGKLsDVVSSQK
S467 GKLsDVVSSQKVWFP
S468 KLsDVVSSQKVWFPV
2349 : Phospho-Chk1 (Ser296) Antibody
12302 : Phospho-Chk1 (Ser317) (D12H3) XP(R) Rabbit mAb
13959 : Phospho-Chk1 (Ser317) (D12H3) XP(R) Rabbit mAb (PE Conjugate)
2341 : Phospho-Chk1 (Ser345) Antibody
2348 : Phospho-Chk1 (Ser345) (133D3) Rabbit mAb
12268 : Phospho-Chk1 (Ser345) (133D3) Rabbit mAb (PE Conjugate)
  rat

 
K132 GITHRDIKPENLLLD
K145 LDERDNLKISDFGLA
T279 GAKRPRATSGGMSES
S280 AKRPRATSGGMSESS
S284 RATSGGMSESSSGFS
S286 TSGGMSESSSGFSKH
S288 GGMSESSSGFSKHIH
S291 SESSSGFSKHIHSNL
K292 ESSSGFSKHIHSNLD
S296 GFSKHIHSNLDFSPI
S301 IHSNLDFSPINSGSS
S305 LDFSPINSGSSEENV
S307 FSPINSGSSEENVKF
S308 SPINSGSSEENVKFS
K313 GSSEENVKFSSSQPE
S315 SEENVKFSSSQPEPR
S316 EENVKFSSSQPEPRT
S317 ENVKFSSSQPEPRTG
T330 TGLSLWDTGPSNVDK
G331 GLSLWDTGPSNVDKL
S333 SLWDTGPSNVDKLVQ
S343 DKLVQGISFSQPTCP
S345 LVQGISFSQPTCPDH
T348 GISFSQPTCPDHMLV
S357 PDHMLVNSQLLGTPG
T362 VNSQLLGTPGSSQNP
S365 QLLGTPGSSQNPWQR
S366 LLGTPGSSQNPWQRL
K383 RMTRFFTKLDADKSY
S389 TKLDADKSYQCLKET
K399 CLKETFEKLGYQWKK
K436 NLVEMDEKILVDFRL
K452 KGDGLEFKRHFLKIK
S463 LKIKGKLSDIVSSQK
S467 GKLSDIVSSQKVWFP
S468 KLSDIVSSQKVWFPV
2349 : Phospho-Chk1 (Ser296) Antibody
2341 : Phospho-Chk1 (Ser345) Antibody
2348 : Phospho-Chk1 (Ser345) (133D3) Rabbit mAb
12268 : Phospho-Chk1 (Ser345) (133D3) Rabbit mAb (PE Conjugate)
  chicken

 
K132 GITHRDLKPENLLLD
K145 LDERDNLKISDFGLA
S279 GTKRGRVSSGGVTEs
S280 TKRGRVSSGGVTEsP
T284 RVSSGGVTEsPGALP
S286-p SSGGVTEsPGALPKH
- gap
- gap
K292 EsPGALPKHIRSDTD
S296 ALPKHIRSDTDFsPV
S301-p IRSDTDFsPVKSALG
S305 TDFsPVKSALGEDKA
- gap
- gap
S313 ALGEDKASYSTSQPE
S315 GEDKASYSTSQPEPG
T316 EDKASYSTSQPEPGT
S317 DKASYSTSQPEPGTG
S330 TGGALWDSSTGSIDR
S331 GGALWDSSTGSIDRL
G333 ALWDSSTGSIDRLVQ
S343 DRLVQGISFsQPACP
S345-p LVQGISFsQPACPEH
A348 GISFsQPACPEHMLL
S357 PEHMLLNSQLLGTPG
T362 LNSQLLGTPGSSQSP
S365 QLLGTPGSSQSPWQR
S366 LLGTPGSSQSPWQRL
K383 RMTRFFTKLDADGSY
S389 TKLDADGSYRSLRDV
K399 SLRDVCEKMGYGWKQ
R436 NLLEMESRILVDFRL
K452 KGDGLEFKRHFLKIK
S463 LKIKGKLSDVVSTQK
S467 GKLSDVVSTQKVWLP
T468 KLSDVVSTQKVWLPP
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