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Protein Page:
MSH6 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MSH6 Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein
Chromosomal Location of Human Ortholog: 2p16
Cellular Component: Golgi apparatus; nuclear chromosome; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; plasma membrane; nucleolus; MutSalpha complex; nucleus
Molecular Function: DNA-dependent ATPase activity; protein homodimerization activity; single thymine insertion binding; ATPase activity; oxidized purine DNA binding; magnesium ion binding; ADP binding; methylated histone residue binding; mismatched DNA binding; protein binding; four-way junction DNA binding; single guanine insertion binding; guanine/thymine mispair binding; double-stranded DNA binding; MutLalpha complex binding; chromatin binding; ATP binding
Biological Process: positive regulation of helicase activity; ATP catabolic process; negative regulation of DNA recombination; mismatch repair; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; somatic hypermutation of immunoglobulin genes; isotype switching; somatic recombination of immunoglobulin gene segments; determination of adult life span; DNA repair; maintenance of DNA repeat elements; response to UV; meiotic mismatch repair
Reference #:  P52701 (UniProtKB)
Alt. Names/Synonyms: DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS homolog 6 (E. coli); MutS-alpha 160 kDa subunit; p160; sperm-associated protein
Gene Symbols: MSH6
Molecular weight: 152,786 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MSH6

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T6 __MSRQSTLysFFPK
0 2 Y8-p MSRQSTLysFFPKsP
0 1 S9-p SRQSTLysFFPKsPA
0 113 S14-p LysFFPKsPALSDAN
0 3 S41-p AAAAPGAsPsPGGDA
0 3 S43-p AAPGAsPsPGGDAAW
0 1 S63 GPRPLARSAsPPKAk
0 3 S65-p RPLARSAsPPKAkNL
0 8 P66 PLARSAsPPKAkNLN
0 1 K70-ac SAsPPKAkNLNGGLR
0 5 S79-p LNGGLRRsVAPAAPt
0 1 - gap
0 1 - gap
0 6 - gap
0 1 T86-p sVAPAAPtsCDFsPG
0 1 S87-p VAPAAPtsCDFsPGD
0 3 S91-p APtsCDFsPGDLVWA
0 25 S137-p HVQFFDDsPtRGWVS
0 5 T139-p QFFDDsPtRGWVSKR
0 1 K149 WVSKRLLKPyTGSkS
0 1 Y151-p SKRLLKPyTGSkSKE
0 2 K155-ac LKPyTGSkSKEAQKG
0 4 S200-p LAVCDEPsEPEEEEE
0 1 T213-p EEMEVGTtYVtDKsE
0 2 T216-p EVGTtYVtDKsEEDN
0 12 S219-p TtYVtDKsEEDNEIE
0 1 T235-p EEEVQPKtQGSRRSS
0 20 S227-p EEDNEIEsEEEVQPK
0 37 S252-p IKKRRVIsDsEsDIG
0 28 S254-p KRRVIsDsEsDIGGs
0 27 S256-p RVIsDsEsDIGGsDV
0 35 S261-p sEsDIGGsDVEFKPD
0 2 T269-p DVEFKPDtKEEGssD
0 5 S274-p PDtKEEGssDEIssG
0 5 S275-p DtKEEGssDEIssGV
0 4 S279-p EGssDEIssGVGDsE
0 4 S280-p GssDEIssGVGDsES
0 3 S285-p IssGVGDsESEGLNs
0 2 S292-p sESEGLNsPVKVARK
0 5 T305-p RKRKRMVtGNGsLKR
0 7 S309-p RMVtGNGsLKRKSSR
0 1 S314 NGsLKRKSSRKETPS
0 1 K324-ub KETPSATkQATSISS
0 1 K334-ub TSISSETkNTLRAFS
0 1 S346 AFSAPQNSEsQAHVS
0 3 S348-p SAPQNSEsQAHVSGG
0 1 - gap
0 1 S353 SEsQAHVSGGGDDSS
0 11 K476-ub YSDSLVQkGYkVARV
0 2 K479-ub SLVQkGYkVARVEQT
0 2 T488-p ARVEQTEtPEMMEAR
0 1 S503 CRKMAHISkYDRVVR
0 1 K504-ac RKMAHISkYDRVVRR
0 9 K519-ub EICRIITkGTQTYSV
0 5 K537-ub DPSENYSkYLLSLkE
0 1 K543-ub SkYLLSLkEKEEDSS
0 1 K598-ub PVQVLFEkGNLSKET
0 4 K632-ub SQFWDASkTLRTLLE
0 2 K646-ub EEEYFREkLSDGIGV
0 1 K660-ub VMLPQVLkGMTSESD
0 1 S702-p LIDQELLsMANFEEy
0 1 Y709-p sMANFEEyIPLDSDT
0 3 K728-ub RSGAIFTkAYQRMVL
0 1 T767-p ERVDTCHtPFGKRLL
0 1 K813-ub SEVVELLkKLPDLER
0 1 K824-ub DLERLLSkIHNVGsP
0 15 S830-p SkIHNVGsPLkSQNH
0 2 K833-ub HNVGsPLkSQNHPDS
0 1 K852-ub YEETTYSkKKIIDFL
0 1 I872 FKVMCKIIGIMEEVA
0 1 K883-ub EEVADGFkSKILKQV
0 2 K885 VADGFkSKILKQVIS
0 1 I886 ADGFkSKILKQVISL
0 2 T895-p KQVISLQtkNPEGRF
0 2 K896-ub QVISLQtkNPEGRFP
0 1 N897 VISLQtkNPEGRFPD
0 1 T914-p VELNRWDtAFDHEKA
0 1 T928-p ARKTGLItPkAGFDs
0 1 K930-ub KTGLItPkAGFDsDY
0 4 S935-p tPkAGFDsDYDQALA
0 1 T1010-p CKRYWTKtIEKKLAN
0 22 T1085-p VILLPEDtPPFLELK
0 1 K1101 SRHPCITKTFFGDDF
0 1 T1219 VDELGRGTATFDGTA
0 1 T1221 ELGRGTATFDGTAIA
0 1 K1233 AIANAVVKELAETIk
0 1 K1240-ub KELAETIkCRTLFST
0 4 K1315-ub LPEEVIQkGHRKARE
0 1 K1352-ub VDAEAVHkLLTLIKE
  mouse

 
T6-p __MSRQStLYSFFPK
Y8 MSRQStLYSFFPKsP
S9 SRQStLYSFFPKsPA
S14-p LYSFFPKsPALGDTK
S38 GAAASGASASRGGDA
S40 AASGASASRGGDAAW
S60-p GSRSAAVsAssPEAK
S62-p RSAAVsAssPEAKDL
S63-p SAAVsAssPEAKDLN
K67 sAssPEAKDLNGGLR
A76 LNGGLRRAsssAQAV
S77-p NGGLRRAsssAQAVP
S78-p GGLRRAsssAQAVPP
S79-p GLRRAsssAQAVPPS
S86 sAQAVPPSSCDFSPG
S87 AQAVPPSSCDFSPGD
S91 PPSSCDFSPGDLVWA
S137-p HVQFFDDsPtRGWVS
T139-p QFFDDsPtRGWVSKR
K149-ac WVSKRMLkPYTGSkS
Y151 SKRMLkPYTGSkSKE
K155-ac LkPYTGSkSKEAQKG
S200-p LAVCDEPsEPEEEEE
A213 EETEVHEAYLsDKsE
S216-p EVHEAYLsDKsEEDN
S219-p EAYLsDKsEEDNYNE
- gap
S227-p EEDNYNEsEEEAQPS
S252-p VKKRRVIsDsEsDIG
S254-p KRRVIsDsEsDIGGs
S256-p RVIsDsEsDIGGsDV
S261-p sEsDIGGsDVEFKPD
T269 DVEFKPDTKQEGSSD
S274 PDTKQEGSSDDASSG
S275 DTKQEGSSDDASSGV
S279 EGSSDDASSGVGDSD
S280 GSSDDASSGVGDSDS
S285 ASSGVGDSDSEDLGT
T292 SDSEDLGTFGKGAPK
V305 PKRKRAMVAQGGLRR
- gap
S314-p QGGLRRKsLKKETGS
K323 KKETGSAKRATPILS
K333 TPILSETKSTLSAFS
S345-p AFSAPQNsEsQtHVs
S347-p SAPQNsEsQtHVsGG
T349-p PQNsEsQtHVsGGGN
S352-p sEsQtHVsGGGNDSS
K475-ub FSDSLVQkGYKVARV
K478 SLVQkGYKVARVEQT
T487 ARVEQTETPEMMEAR
S502-p CRKMAHVsKFDRVVR
K503 RKMAHVsKFDRVVRR
K518 EICRIITKGTQTYSV
R536 DPSENYSRYLLSLKE
K542 SRYLLSLKEKEEETS
K597 PVQILFEKGNLSTET
K631 SQFWDATKTLRTLLE
- gap
K657 TVLPLVLKGMTSESD
S699 LIDQELLSMANFEEY
Y706 SMANFEEYFPLDSDT
K725-ub KPGAVFTkASQRMVL
T764 ERLDTCHTPFGKRLL
K810 TEVADLLKKLPDLER
K821 DLERLLSKIHNVGsP
S827-p SKIHNVGsPLKSQNH
K830 HNVGsPLKSQNHPDS
K849 YEETTYSKKKIIDFL
S869-p FKVMCKVsGLLEEVA
T880 EEVAGGFTSktLKQV
K882-ub VAGGFTSktLKQVVT
T883-p AGGFTSktLKQVVTL
S892-p KQVVTLQsKsPKGRF
K893 QVVTLQsKsPKGRFP
S894-p VVTLQsKsPKGRFPD
T911 AELQRWDTAFDHEKA
T925 ARKTGLITPKAGFDs
K927 KTGLITPKAGFDsDY
S932-p TPKAGFDsDYDQALA
T1007 CKRYWTKTIEKKLAN
T1083 IVLPGEDTHPFLEFK
K1099-ub SRHPCITkTFFGDDF
T1217-p VDELGRGtAtFDGTA
T1219-p ELGRGtAtFDGTAIA
K1231-ub AIANAVVkELAETIK
K1238 kELAETIKCRTLFST
K1313 LPEEVIQKGHRKARE
R1350 INGEAIHRLLALING
  rat

 
T6 __MSRQSTLYSFFPK
Y8 MSRQSTLYSFFPKSP
S9 SRQSTLYSFFPKSPA
S14 LYSFFPKSPALGDTK
S38 GAAASGASASRGRDA
S40 AASGASASRGRDAAW
S60 GSRPAALSTSLPEAK
S62 RPAALSTSLPEAKDL
L63 PAALSTSLPEAKDLN
K67 STSLPEAKDLNGGLR
S76 LNGGLRRSAAPAVPA
- gap
- gap
- gap
S84 AAPAVPASSCDFSPG
S85 APAVPASSCDFSPGD
S89 PASSCDFSPGDLVWA
S135-p HVQFFDDsPTRGWVS
T137 QFFDDsPTRGWVSKR
K147 WVSKRMLKPYTGSKS
Y149 SKRMLKPYTGSKSKE
K153 LKPYTGSKSKEAQKG
S198 LAVCDEPSEPEEEEE
A216 EETQVHGAYLSDKSE
S219 QVHGAYLSDKSEEEN
S222 GAYLSDKSEEENQDE
- gap
S230 EEENQDESEEETQPN
S255-p VKKRRVIsDsEsDIG
S257-p KRRVIsDsEsDIGGs
S259-p RVIsDsEsDIGGsDV
S264-p sEsDIGGsDVEFKPD
S272 DVEFKPDSKQEGssD
S277-p PDSKQEGssDEVSSG
S278-p DSKQEGssDEVSSGV
S282 EGssDEVSSGVGDSD
S283 GssDEVSSGVGDSDS
S288 VSSGVGDSDSEGLGS
S295 SDSEGLGSFGKGAPK
A308 PKRKRTVAAHGGLKR
- gap
S317 HGGLKRKSLKKETGS
K326 KKETGSAKQATRILS
K336 TRILSETKSTLSAFC
S348 AFCAPQNSESQTHVC
S350 CAPQNSESQTHVCGG
T352 PQNSESQTHVCGGGN
C355 SESQTHVCGGGNDSS
K478 FSDSLVQKGYKVARV
K481 SLVQKGYKVARVEQT
T490 ARVEQTETPEMMEAR
S505 CRKMAHVSKFDRVVR
K506 RKMAHVSKFDRVVRR
K521 EICRIITKGTQTYSV
R539 DPSENYSRYLLSLKE
K545 SRYLLSLKEKEEDSS
K600 PVQILFEKGNLSTET
K634 SQFWDATKTLRTLLE
- gap
K660 AELPPVLKAMTSESD
S702 LIDQELLSMANFEEY
Y709 SMANFEEYFPLDSDM
K728 KPGAVFTKASQRMVL
T767 ERLDTCHTPFGKRLL
K813 AEVADLLKKLPDLER
K824 DLERLLSKIHNVGsP
S830-p SKIHNVGsPLKSQNH
K833 HNVGsPLKSQNHPDT
K852 YEETTYSKKKIIDFL
T872 FKVMCKITRLMEDVA
K883 EDVADGFKSKTLKQV
K885 VADGFKSKTLKQVVT
T886 ADGFKSKTLKQVVTL
T895 KQVVTLQTKSPKGRF
K896 QVVTLQTKSPKGRFP
S897 VVTLQTKSPKGRFPD
T914 AELNRWDTAFDHEKA
T928 ARKTGLITPKAGFDs
K930 KTGLITPKAGFDsDY
S935-p TPKAGFDsDYDQALA
V1010 CKRYWTKVIEKKLSN
T1086 LLLPGEDTHPFLELK
K1102 SRHPCITKTFFGDDF
T1220 VDELGRGTATFDGTA
T1222 ELGRGTATFDGTAIA
K1234 AIASAVVKELAETIK
K1241 KELAETIKCRTLFST
K1316 LPEEVIQKGHRKARE
R1353 VNSEAIHRLLALVNQ
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