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Protein Page:
MSH6 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
MSH6 Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein
Cellular Component: Golgi apparatus; nuclear chromosome; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; nucleolus; plasma membrane; MutSalpha complex; nucleus
Molecular Function: DNA-dependent ATPase activity; protein homodimerization activity; single thymine insertion binding; ATPase activity; oxidized purine DNA binding; magnesium ion binding; ADP binding; methylated histone residue binding; mismatched DNA binding; protein binding; four-way junction DNA binding; single guanine insertion binding; double-stranded DNA binding; guanine/thymine mispair binding; MutLalpha complex binding; chromatin binding; ATP binding
Biological Process: ATP catabolic process; negative regulation of DNA recombination; mismatch repair; somatic hypermutation of immunoglobulin genes; isotype switching; determination of adult life span; DNA repair; maintenance of DNA repeat elements; meiotic mismatch repair; positive regulation of helicase activity; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; somatic recombination of immunoglobulin gene segments; response to UV
Reference #:  P52701 (UniProtKB)
Alt. Names/Synonyms: DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS homolog 6 (E. coli); MutS-alpha 160 kDa subunit; p160; sperm-associated protein
Gene Symbols: MSH6
Molecular weight: 152,786 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MSH6

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 108 S14-p LYSFFPKsPALSDAN
0 2 S41-p AAAAPGAsPsPGGDA
0 3 S43-p AAPGAsPsPGGDAAW
0 2 S65-p RPLARSAsPPKAkNL
0 7 P66 PLARSAsPPKAkNLN
0 1 K70-a SAsPPKAkNLNGGLR
0 3 S79-p LNGGLRRsVAPAAPT
0 5 - gap
0 1 S91-p APTSCDFsPGDLVWA
0 20 S137-p HVQFFDDsPtRGWVS
0 3 T139-p QFFDDsPtRGWVSKR
0 2 K155-a LKPYTGSkSKEAQKG
0 3 S200-p LAVCDEPsEPEEEEE
0 1 T213-p EEMEVGTtYVtDKsE
0 1 T216-p EVGTtYVtDKsEEDN
0 9 S219-p TtYVtDKsEEDNEIE
0 16 S227-p EEDNEIEsEEEVQPK
0 31 S252-p IKKRRVIsDsEsDIG
0 22 S254-p KRRVIsDsEsDIGGs
0 21 S256-p RVIsDsEsDIGGsDV
0 30 S261-p sEsDIGGsDVEFKPD
0 1 T269-p DVEFKPDtKEEGssD
0 3 S274-p PDtKEEGssDEIssG
0 3 S275-p DtKEEGssDEIssGV
0 3 S279-p EGssDEIssGVGDsE
0 3 S280-p GssDEIssGVGDsES
0 1 S285-p IssGVGDsESEGLNS
0 4 T305-p RKRKRMVtGNGsLKR
0 5 S309-p RMVtGNGsLKRKSSR
0 1 K324-u KETPSATkQATSISS
0 1 K334-u TSISSETkNTLRAFS
0 1 S346 AFSAPQNSEsQAHVS
0 3 S348-p SAPQNSEsQAHVSGG
0 1 - gap
0 1 S353 SEsQAHVSGGGDDSS
0 11 K476-u YSDSLVQkGYkVARV
0 2 K479-u SLVQkGYkVARVEQT
0 2 T488-p ARVEQTEtPEMMEAR
0 1 K504-a RKMAHISkYDRVVRR
0 9 K519-u EICRIITkGTQTYSV
0 5 K537-u DPSENYSkYLLSLKE
0 1 K598-u PVQVLFEkGNLSKET
0 4 K632-u SQFWDASkTLRTLLE
0 2 K646-u EEEYFREkLSDGIGV
0 1 K660-u VMLPQVLkGMTSESD
0 3 K728-u RSGAIFTkAYQRMVL
0 1 K813-u SEVVELLkKLPDLER
0 1 K824-u DLERLLSkIHNVGsP
0 10 S830-p SkIHNVGsPLkSQNH
0 2 K833-u HNVGsPLkSQNHPDS
0 1 K852-u YEETTYSkKKIIDFL
0 1 I872 FKVMCKIIGIMEEVA
0 1 K883-u EEVADGFkSKILKQV
0 2 K885 VADGFkSKILKQVIS
0 1 I886 ADGFkSKILKQVISL
0 2 T895-p KQVISLQtkNPEGRF
0 2 K896-u QVISLQtkNPEGRFP
0 1 N897 VISLQtkNPEGRFPD
0 1 T928-p ARKTGLItPkAGFDs
0 1 K930-u KTGLItPkAGFDsDY
0 3 S935-p tPkAGFDsDYDQALA
0 1 T1010-p CKRYWTKtIEKKLAN
0 22 T1085-p VILLPEDtPPFLELK
0 1 T1219 VDELGRGTATFDGTA
0 1 T1221 ELGRGTATFDGTAIA
0 4 K1315-u LPEEVIQkGHRKARE
0 1 K1352-u VDAEAVHkLLTLIKE
  mouse

 
S14-p LYSFFPKsPALGDTK
S38 GAAASGASASRGGDA
S40 AASGASASRGGDAAW
S62-p RSAAVSAssPEAKDL
S63-p SAAVSAssPEAKDLN
K67 SAssPEAKDLNGGLR
A76 LNGGLRRASSsAQAV
S79-p GLRRASSsAQAVPPS
S91 PPSSCDFSPGDLVWA
S137-p HVQFFDDsPtRGWVS
T139-p QFFDDsPtRGWVSKR
K155-a LKPYTGSkSKEAQKG
S200-p LAVCDEPsEPEEEEE
A213 EETEVHEAYLsDKsE
S216-p EVHEAYLsDKsEEDN
S219-p EAYLsDKsEEDNYNE
S227-p EEDNYNEsEEEAQPS
S252-p VKKRRVIsDsEsDIG
S254-p KRRVIsDsEsDIGGs
S256-p RVIsDsEsDIGGsDV
S261-p sEsDIGGsDVEFKPD
T269 DVEFKPDTKQEGSSD
S274 PDTKQEGSSDDASSG
S275 DTKQEGSSDDASSGV
S279 EGSSDDASSGVGDSD
S280 GSSDDASSGVGDSDS
S285 ASSGVGDSDSEDLGT
- gap
- gap
K323 KKETGSAKRATPILS
K333 TPILSETKSTLSAFS
S345-p AFSAPQNsEsQtHVs
S347-p SAPQNsEsQtHVsGG
T349-p PQNsEsQtHVsGGGN
S352-p sEsQtHVsGGGNDSS
K475-u FSDSLVQkGYKVARV
K478 SLVQkGYKVARVEQT
T487 ARVEQTETPEMMEAR
K503 RKMAHVSKFDRVVRR
K518 EICRIITKGTQTYSV
R536 DPSENYSRYLLSLKE
K597 PVQILFEKGNLSTET
K631 SQFWDATKTLRTLLE
- gap
K657 TVLPLVLKGMTSESD
K725-u KPGAVFTkASQRMVL
K810 TEVADLLKKLPDLER
K821 DLERLLSKIHNVGsP
S827-p SKIHNVGsPLKSQNH
K830 HNVGsPLKSQNHPDS
K849 YEETTYSKKKIIDFL
S869-p FKVMCKVsGLLEEVA
T880 EEVAGGFTSktLKQV
K882-u VAGGFTSktLKQVVT
T883-p AGGFTSktLKQVVTL
S892-p KQVVTLQsKsPKGRF
K893 QVVTLQsKsPKGRFP
S894-p VVTLQsKsPKGRFPD
T925 ARKTGLITPKAGFDs
K927 KTGLITPKAGFDsDY
S932-p TPKAGFDsDYDQALA
T1007 CKRYWTKTIEKKLAN
T1083 IVLPGEDTHPFLEFK
T1217-p VDELGRGtAtFDGTA
T1219-p ELGRGtAtFDGTAIA
K1313 LPEEVIQKGHRKARE
R1350 INGEAIHRLLALING
  rat

 
S14 LYSFFPKSPALGDTK
S38 GAAASGASASRGRDA
S40 AASGASASRGRDAAW
S62 RPAALSTSLPEAKDL
L63 PAALSTSLPEAKDLN
K67 STSLPEAKDLNGGLR
S76 LNGGLRRSAAPAVPA
- gap
S89 PASSCDFSPGDLVWA
S135 HVQFFDDSPTRGWVS
T137 QFFDDSPTRGWVSKR
K153 LKPYTGSKSKEAQKG
S198 LAVCDEPSEPEEEEE
A216 EETQVHGAYLSDKSE
S219 QVHGAYLSDKSEEEN
S222 GAYLSDKSEEENQDE
S230 EEENQDESEEETQPN
S255-p VKKRRVIsDSESDIG
S257 KRRVIsDSESDIGGs
S259 RVIsDSESDIGGsDV
S264-p SESDIGGsDVEFKPD
S272 DVEFKPDSKQEGSSD
S277 PDSKQEGSSDEVSSG
S278 DSKQEGSSDEVSSGV
S282 EGSSDEVSSGVGDSD
S283 GSSDEVSSGVGDSDS
S288 VSSGVGDSDSEGLGS
- gap
- gap
K326 KKETGSAKQATRILS
K336 TRILSETKSTLSAFC
S348 AFCAPQNSESQTHVC
S350 CAPQNSESQTHVCGG
T352 PQNSESQTHVCGGGN
C355 SESQTHVCGGGNDSS
K478 FSDSLVQKGYKVARV
K481 SLVQKGYKVARVEQT
T490 ARVEQTETPEMMEAR
K506 RKMAHVSKFDRVVRR
K521 EICRIITKGTQTYSV
R539 DPSENYSRYLLSLKE
K600 PVQILFEKGNLSTET
K634 SQFWDATKTLRTLLE
- gap
K660 AELPPVLKAMTSESD
K728 KPGAVFTKASQRMVL
K813 AEVADLLKKLPDLER
K824 DLERLLSKIHNVGSP
S830 SKIHNVGSPLKSQNH
K833 HNVGSPLKSQNHPDT
K852 YEETTYSKKKIIDFL
T872 FKVMCKITRLMEDVA
K883 EDVADGFKSKTLKQV
K885 VADGFKSKTLKQVVT
T886 ADGFKSKTLKQVVTL
T895 KQVVTLQTKSPKGRF
K896 QVVTLQTKSPKGRFP
S897 VVTLQTKSPKGRFPD
T928 ARKTGLITPKAGFDS
K930 KTGLITPKAGFDSDY
S935 TPKAGFDSDYDQALA
V1010 CKRYWTKVIEKKLSN
T1086 LLLPGEDTHPFLELK
T1220 VDELGRGTATFDGTA
T1222 ELGRGTATFDGTAIA
K1316 LPEEVIQKGHRKARE
R1353 VNSEAIHRLLALVNQ
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