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Protein Page:
TCF8 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TCF8 Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; DNA binding protein; Transcription, coactivator/corepressor; C2H2-type zinc finger protein
Cellular Component: transcription factor complex; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor activity; transcription factor binding; transcription corepressor activity
Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of mesenchymal cell proliferation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent
Reference #:  P37275 (UniProtKB)
Alt. Names/Synonyms: AREB6; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; MGC133261; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; TCF-8; TCF8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein
Gene Symbols: ZEB1
Molecular weight: 124,074 Da
Basal Isoelectric point: 4.87  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/├č-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TCF8

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 3 S31-p NTVVETNsDsDDEDK
0 4 S33-p VVETNsDsDDEDKLH
0 1 T151-p EEDQRQGtPEAsGHD
0 1 S155-p RQGtPEAsGHDENGt
0 2 T162-p sGHDENGtPDAFSQL
0 4 S263-p KEHLRIHsGEKPYEC
0 3 S309-p PRTGLKTsQCSsPsL
0 6 S313-p LKTsQCSsPsLsAsP
0 6 S315-p TsQCSsPsLsAsPGs
0 1 S317-p QCSsPsLsAsPGsPt
0 4 S319-p SsPsLsAsPGsPtRP
0 22 S322-p sLsAsPGsPtRPQIR
0 4 T324-p sAsPGsPtRPQIRQK
0 1 S342-p KPLQEQLsVNQIKTE
0 1 T443-p LASKEQEtINAsPIQ
0 2 S447-p EQEtINAsPIQQGGH
0 1 S455-p PIQQGGHsVISAISL
0 1 K504-ub PVATNSCkSEKLPED
0 1 S516-p PEDLTVKsEKDKsFE
0 2 S521-p VKsEKDKsFEGGVND
0 1 S529-p FEGGVNDsTCLLCDD
0 3 S583-p ATGDGNLsPSQPPLK
0 2 S641-p AGQISVQssEPssPE
0 1 S642-p GQISVQssEPssPEP
0 1 S645-p SVQssEPssPEPGKV
0 6 S646-p VQssEPssPEPGKVN
0 9 S679-p DSTVNLQsPLKMTNs
0 1 T684 LQsPLKMTNsPVLPV
0 2 N685 QsPLKMTNsPVLPVG
0 2 S686-p sPLKMTNsPVLPVGS
0 2 S693 sPVLPVGSTTNGSRs
0 6 S700-p STTNGSRsstPsPSP
0 4 S701-p TTNGSRsstPsPSPL
0 5 T702-p TNGSRsstPsPSPLN
0 3 P703 NGSRsstPsPSPLNL
0 10 S704-p GSRsstPsPSPLNLS
0 2 S706 RsstPsPSPLNLSSS
0 1 S711 sPSPLNLSSSRNtQG
0 1 T716-p NLSSSRNtQGYLYTA
0 14 S887-p NVEDQNDsDstPPKK
0 3 S889-p EDQNDsDstPPKKKM
0 5 T890-p DQNDsDstPPKKKMR
0 1 S995-p REAEERDstEQEEAG
0 1 T996-p EAEERDstEQEEAGP
0 1 S1018-p VGARASPsQGDsDER
0 1 S1022-p ASPsQGDsDERESLT
0 4 S1100-p MKDDRAEsQASSLGQ
  mouse

 
S31-p NTVVEANsDsDDEDK
S33-p VVEANsDsDDEDKLH
T131 EEDQRQGTPEASSHD
S135 RQGTPEASSHDENGT
T142 SSHDENGTPDAFSQL
S243 KEHLRIHSGEKPYEC
S289 PRSGLKTSQCSsPsL
S293-p LKTSQCSsPsLSTsP
S295-p TSQCSsPsLSTsPGs
S297 QCSsPsLSTsPGsPt
S299-p SsPsLSTsPGsPtRP
S302-p sLSTsPGsPtRPQIR
T304-p STsPGsPtRPQIRQK
S322 KPLQEPLSVNQIKTE
A423 LASKEQEAVSASPIQ
S427 EQEAVSASPIQQGGH
S435 PIQQGGHSVISAISL
K484 PAPTNSCKSEKLPED
S496 PEDLTVKSETDKSFE
S501 VKSETDKSFEGARDD
S509 FEGARDDSTCLLCED
S561 SAGNGDLSPSQPPLK
S619-p AGQIPGQsPDPPsPG
- gap
- gap
S624-p GQsPDPPsPGTGSVN
S657-p EDSTRGQsPVKIRss
R662 GQsPVKIRssPVLPV
S663-p QsPVKIRssPVLPVG
S664-p sPVKIRssPVLPVGs
S671-p sPVLPVGsAMNGSRs
S678-p sAMNGSRsCtssPsP
C679 AMNGSRsCtssPsPL
T680-p MNGSRsCtssPsPLN
S681-p NGSRsCtssPsPLNL
S682-p GSRsCtssPsPLNLC
S684-p RsCtssPsPLNLCSA
C689 sPsPLNLCSARNPQG
P694 NLCSARNPQGYSCVA
S865-p TVEDQNDsDSTPPKK
S867 EDQNDsDSTPPKKKT
T868 DQNDsDSTPPKKKTR
A973 RGAEDRDAMEQEDAG
M974 GAEDRDAMEQEDAGP
S1000 VGARASPSQADSDER
S1004 ASPSQADSDERESLT
Q1086 TVEVGAAQQAGSLEQ
  rat

 
S31-p NTVVEANsDsDDEDK
S33-p VVEANsDsDDEDKLH
T131 EEDQRQGTPEASGHD
S135 RQGTPEASGHDDNGT
T142 SGHDDNGTPDAFSQL
S243 KEHLRIHSGEKPYEC
S289 PRSGLKTSQCSSPSL
S293 LKTSQCSSPSLSTSP
S295 TSQCSSPSLSTSPGS
S297 QCSSPSLSTSPGSPT
S299 SSPSLSTSPGSPTRP
S302 SLSTSPGSPTRPQIR
T304 STSPGSPTRPQIRQK
S322 KPLQEPLSVNQIKTE
A423 LASKEQEAVSASSIQ
S427 EQEAVSASSIQQGGH
S435 SIQQGGHSVISAISL
K484 PAPPKSCKSEKSPED
S496 PEDLTVKSEKDKSFD
S501 VKSEKDKSFDGAADE
S509 FDGAADESTCLLCED
S561 SAGNGDLSPSQPPLK
S619 AGQIPGQSLEPPsPG
- gap
- gap
S624-p GQSLEPPsPGPGSGN
S657 EDSTRGQSPLKMtsS
T662-p GQSPLKMtsSPVLPV
S663-p QSPLKMtsSPVLPVG
S664 SPLKMtsSPVLPVGs
S671-p SPVLPVGsAINGSRs
S678-p sAINGSRsCTSsPSP
C679 AINGSRsCTSsPSPL
T680 INGSRsCTSsPSPLN
S681 NGSRsCTSsPSPLNL
S682-p GSRsCTSsPSPLNLs
S684 RsCTSsPSPLNLsSA
S689-p sPSPLNLsSARNPQG
P694 NLsSARNPQGYSCVS
S864 SVEDQNDSDCTPPKK
C866 EDQNDSDCTPPKKKT
T867 DQNDSDCTPPKKKTR
A972 RGAEDRDAMEQEDTG
M973 GAEDRDAMEQEDTGP
S999 VGARASPSQADsDER
S1003-p ASPSQADsDERESLT
Q1084 AVEEEAAQQAGSFQQ
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