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Protein Page:
Restin (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Restin Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking. Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts (via zinc finger) with DCTN1. Interacts with MAPRE1 and MAPRE3. Detected in dendritic cells. Highly expressed in the Reed-Sternberg cells of Hodgkin disease. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein
Cellular Component: microtubule cytoskeleton; kinetochore; ruffle; microtubule; centrosome; cytoplasmic vesicle membrane; cytoplasmic microtubule; intermediate filament; cytosol; endosome
Molecular Function: tubulin binding; microtubule plus-end binding; protein binding; protein homodimerization activity; nucleic acid binding; zinc ion binding; metal ion binding; microtubule binding
Biological Process: mitosis; transport; positive regulation of microtubule polymerization; mitotic cell cycle; microtubule bundle formation
Reference #:  P30622 (UniProtKB)
Alt. Names/Synonyms: CAP-GLY domain containing linker protein 1; CAP-Gly domain-containing linker protein 1; CLIP; CLIP-170; CLIP1; CLIP170; CYLN1; cytoplasmic linker 1; Cytoplasmic linker protein 1; Cytoplasmic linker protein 170 alpha-2; MGC131604; Reed-Steinberg cell-expressed intermediate filament-associated protein; Reed-Sternberg intermediate filament-associated protein; Restin; restin (Reed-Steinberg cell-expressed intermediate filament-associated protein); RSN
Gene Symbols: CLIP1
Molecular weight: 162,246 Da
Basal Isoelectric point: 5.29  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Restin

Protein Structure Not Found.


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Sites Implicated In
cytoskeletal reorganization: S312‑p
intracellular localization: S195‑p
molecular association, regulation: S312‑p
phosphorylation: S195‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 T25-p PGSTALKtPTAVVAP
0 1 S43-p TISSEKAsstPssEt
0 2 S44-p ISSEKAsstPssEtQ
0 6 T45-p SSEKAsstPssEtQE
0 1 S47-p EKAsstPssEtQEEF
0 3 S48-p KAsstPssEtQEEFV
0 2 T50-p sstPssEtQEEFVDD
0 5 T140-p EANGLQTtPAsRAts
0 6 S143-p GLQTtPAsRAtsPLC
0 2 T146-p TtPAsRAtsPLCTST
0 9 S147-p tPAsRAtsPLCTSTA
0 1 C150 sRAtsPLCTSTASMV
0 1 T151 RAtsPLCTSTASMVs
0 1 S158-p TSTASMVsSsPstPS
0 1 S160-p TASMVsSsPstPSNI
0 1 S162-p SMVsSsPstPSNIPQ
0 1 T163-p MVsSsPstPSNIPQK
0 6 S172-p SNIPQKPsQPAAKEP
0 2 S180-p QPAAKEPsAtPPIsN
0 15 T182-p AAKEPsAtPPIsNLT
0 3 S186-p PsAtPPIsNLTKtAs
0 2 T191-p PIsNLTKtAsEsIsN
0 5 S193-p sNLTKtAsEsIsNLs
2 60 S195-p LTKtAsEsIsNLsEA
0 8 S197-p KtAsEsIsNLsEAGs
0 73 S200-p sEsIsNLsEAGsIKK
0 41 S204-p sNLsEAGsIKKGERE
0 1 Y262-p GAVAGTRyFQCQPKy
0 1 Y269-p yFQCQPKyGLFAPVH
0 1 T279-p FAPVHKVtKIGFPst
0 3 S285-p VtKIGFPsttPAKAK
0 2 T286-p tKIGFPsttPAKAKA
3 4 T287-p KIGFPsttPAKAKAN
0 1 S306-p VMATTSAsLKRsPsA
1 6 S310-p TSAsLKRsPsASsLs
3 5 S312-p AsLKRsPsASsLsSM
1 2 S314 LKRsPsASsLsSMsS
1 3 S315-p KRsPsASsLsSMsSV
0 1 S317-p sPsASsLsSMsSVAS
1 2 S320-p ASsLsSMsSVASsVS
0 1 S324 sSMsSVASsVSsRPS
0 3 S325-p SMsSVASsVSsRPSR
0 1 S327 sSVASsVSsRPSRtG
0 2 S328-p SVASsVSsRPSRtGL
0 2 S331 SsVSsRPSRtGLLTE
0 1 T333-p VSsRPSRtGLLTETS
1 26 S348-p SRYARKIsGttALQE
0 3 T350-p YARKIsGttALQEAL
0 4 T351-p ARKIsGttALQEALK
0 1 T415 AKMDQLRTMVEAADR
0 2 S510-p VATVSEKsRIMELEK
0 1 S607-p KENESLKsKLEHANK
0 2 K719 AIRSKLDKAEDQHLV
0 1 T731 HLVEMEDTLNKLQEA
0 1 E770 TSQLKATEEKLLDLD
0 2 S783-p LDALRKAssEGKsEM
0 2 S784-p DALRKAssEGKsEMK
0 1 S788-p KAssEGKsEMKKLRQ
0 1 S815-p EIEKNAEsSKASSIT
0 3 T833-p QGRELKLtNLQENLs
0 1 S840-p tNLQENLsEVsQVKE
0 1 S843-p QENLsEVsQVKEtLE
0 1 T848-p EVsQVKEtLEKELQI
0 1 K921 EREEQLIKAKEKLEN
0 2 D957 ELRLKERDVEELQLK
0 1 K1007-ac EKKELERkLsDLEkK
0 2 S1009-p KELERkLsDLEkKME
0 1 K1013-ac RkLsDLEkKMETSHN
0 1 S1066-p KGAREENsGLLQELE
0 1 R1076 LQELEELRKQADKAK
0 1 T1087-p DKAKAAQtAEDAMQI
0 1 T1125 KLQNELDTLKENNLK
0 1 S1140-p NVEELNKsKELLTVE
0 1 S1169-p KQAAAQKsQQLSALQ
0 2 S1230-p DADEEKAsLQKsIsI
0 3 S1234-p EKAsLQKsIsITsAL
0 5 S1236-p AsLQKsIsITsALLT
0 1 S1239-p QKsIsITsALLTEKD
0 1 T1243 sITsALLTEKDAELE
0 8 S1302-p KENKRQLssssGNtD
0 2 S1303-p ENKRQLssssGNtDt
0 4 S1304-p NKRQLssssGNtDtQ
0 4 S1305-p KRQLssssGNtDtQA
0 4 T1308-p LssssGNtDtQADED
0 1 T1310-p sssGNtDtQADEDER
2 4 S1364-p DDLNNYDsDDQEKQS
  Restin iso3  
T25 PGSTALKTPTAVVAP
S43 TISSEKASSTPSSET
S44 ISSEKASSTPSSETQ
T45 SSEKASSTPSSETQE
S47 EKASSTPSSETQEEF
S48 KASSTPSSETQEEFV
T50 SSTPSSETQEEFVDD
T140 EANGLQTTPASRATS
S143 GLQTTPASRATSPLC
T146 TTPASRATSPLCTST
S147 TPASRATSPLCTSTA
C150 SRATSPLCTSTASMV
T151 RATSPLCTSTASMVS
S158 TSTASMVSSSPSTPS
S160 TASMVSSSPSTPSNI
S162 SMVSSSPSTPSNIPQ
T163 MVSSSPSTPSNIPQK
S172 SNIPQKPSQPAAKEP
S180 QPAAKEPSATPPISN
T182 AAKEPSATPPISNLT
S186 PSATPPISNLTKTAS
T191 PISNLTKTASEsISN
S193 SNLTKTASEsISNLS
S195-p LTKTASEsISNLSEA
S197 KTASEsISNLSEAGS
S200 SEsISNLSEAGSIKK
S204 SNLSEAGSIKKGERE
Y262 GAVAGTRYFQCQPKY
Y269 YFQCQPKYGLFAPVH
T279 FAPVHKVTKIGFPST
S285 VTKIGFPSTtPAKAK
T286 TKIGFPSTtPAKAKA
T287-p KIGFPSTtPAKAKAN
S306 VMATTSASLKRSPSA
S310 TSASLKRSPSASSLS
S312 ASLKRSPSASSLSSM
S314 LKRSPSASSLSSMSS
S315 KRSPSASSLSSMSSV
S317 SPSASSLSSMSSVAS
S320 ASSLSSMSSVASSVS
S324 SSMSSVASSVSSRPS
S325 SMSSVASSVSSRPSR
S327 SSVASSVSSRPSRTG
S328 SVASSVSSRPSRTGL
S331 SSVSSRPSRTGLLTE
T333 VSSRPSRTGLLTETS
S348 SRYARKISGTTALQE
T350 YARKISGTTALQEAL
T351 ARKISGTTALQEALK
T415 AKMDQLRTMVEAADR
S464 VATVSEKSRIMELEK
S561 KENESLKSKLEHANK
K673 AIRSKLDKAEDQHLV
T685 HLVEMEDTLNKLQEA
E724 TSQLKATEEKLLDLD
S737 LDALRKASSEGKSEM
S738 DALRKASSEGKSEMK
S742 KASSEGKSEMKKLRQ
S769 EIEKNAESSKASSIT
T787 QGRELKLTNLQENLS
S794 TNLQENLSEVSQVKE
S797 QENLSEVSQVKETLE
T802 EVSQVKETLEKELQI
K875 EREEQLIKAKEKLEN
D911 ELRLKERDVEELQLK
K961 EKKELERKLSDLEKK
S963 KELERKLSDLEKKME
K967 RKLSDLEKKMETSHN
S1020 KGAREENSGLLQELE
R1030 LQELEELRKQADKAK
T1041 DKAKAAQTAEDAMQI
T1079 KLQNELDTLKENNLK
S1094 NVEELNKSKELLTVE
S1123 KQAAAQKSQQLSALQ
S1184 DADEEKASLQKSISI
S1188 EKASLQKSISITSAL
S1190 ASLQKSISITSALLT
S1193 QKSISITSALLTEKD
T1197 SITSALLTEKDAELE
S1256 KENKRQLSSSSGNTD
S1257 ENKRQLSSSSGNTDT
S1258 NKRQLSSSSGNTDTQ
S1259 KRQLSSSSGNTDTQA
T1262 LSSSSGNTDTQADED
T1264 SSSGNTDTQADEDER
S1318-p DDLNNYDsDDQEKQS
  mouse

 
T25 PGSTALKTPAAAAAP
S43 TIPSEKASGPPSSEt
G44 IPSEKASGPPSSEtQ
P45 PSEKASGPPSSEtQE
S47 EKASGPPSSEtQEEF
S48 KASGPPSSEtQEEFV
T50-p SGPPSSEtQEEFVDD
A140 EANGLQAAPGRtAsP
- gap
T144-p LQAAPGRtAsPLstA
S146-p AAPGRtAsPLstAAA
S149-p GRtAsPLstAAATMV
T150-p RtAsPLstAAATMVS
S157 tAAATMVSSSPATPS
S159 AATMVSSSPATPSNI
A161 TMVSSSPATPSNIPH
T162 MVSSSPATPSNIPHK
S171 SNIPHKPSQSTAKEP
S179 QSTAKEPSATPQISN
T181 TAKEPSATPQISNLT
S185 PSATPQISNLTKtAs
T190-p QISNLTKtAsEsIsN
S192-p SNLTKtAsEsIsNLs
S194-p LTKtAsEsIsNLsEA
S196-p KtAsEsIsNLsEAGs
S199-p sEsIsNLsEAGsVKK
S203-p sNLsEAGsVKKGERE
Y261 GAVAGTRYFQCQPKY
Y268 YFQCQPKYGLFAPVH
T278 FAPVHKVTKIGFPsT
S284-p VTKIGFPsTtPAKAK
T285 TKIGFPsTtPAKAKA
T286-p KIGFPsTtPAKAKAA
S305 VMAATPASLKRsPsA
S309-p TPASLKRsPsAssLs
S311-p ASLKRsPsAssLsSM
S313-p LKRsPsAssLsSMSS
S314-p KRsPsAssLsSMSSV
S316-p sPsAssLsSMSSVAs
S319 AssLsSMSSVAssVS
S323-p sSMSSVAssVSSKPs
S324-p SMSSVAssVSSKPsR
S326 SSVAssVSSKPsRTG
S327 SVAssVSSKPsRTGL
S330-p ssVSSKPsRTGLLTE
T332 VSSKPsRTGLLTETS
S347-p SRYARKIsGttALQE
T349-p YARKIsGttALQEAL
T350-p ARKIsGttALQEALK
T414-p AKMDQLRtMVEAADR
S463-p VATVSEKsRIMELEK
S560 KENESLRSKLDHANK
S672-p AVKARLDsAEDQHLV
T684-p HLVEMEDtLNKLQEA
K723-ub TSQLSAVkEKLLDLD
N736 LDALRKANSEGKLEL
S737 DALRKANSEGKLELE
L741 KANSEGKLELETLRQ
S768 ETERNAESSKANSIT
T786 QEKELVLTGLQDSLN
N793 TGLQDSLNQVNQVKE
N796 QDSLNQVNQVKETLE
T801 QVNQVKETLEKELQT
K874-ac DREDQLVkAKEKLEN
S910-p ELRLKERsVEELQLK
K960 EKKELEEKLLELEKK
L962 KELEEKLLELEKKME
K966 EKLLELEKKMETSYN
R1019 KAAQEANRDLMQDME
K1029-ac MQDMEELkTQADKAK
T1040 DKAKAAQTAEDAMQI
T1078-p RLQNELDtLKENNLK
S1093 TVEELNKSKELLSVE
S1122 KQAAAQKSQQLSALQ
S1183-p DADEEKAsLQKsIsL
S1187-p EKAsLQKsIsLTSAL
S1189-p AsLQKsIsLTSALLt
S1192 QKsIsLTSALLtEKD
T1196-p sLTSALLtEKDAELE
S1255-p KENKRQLsSSsGNtD
S1256 ENKRQLsSSsGNtDA
S1257 NKRQLsSSsGNtDAQ
S1258-p KRQLsSSsGNtDAQA
T1261-p LsSSsGNtDAQAEED
A1263 SSsGNtDAQAEEDER
S1317-p EDLNSYDsDDQEKQS
  rat

 
T25 PGSTALKTPAAAAAP
S43 TVPSEKASGPPSSET
G44 VPSEKASGPPSSETQ
P45 PSEKASGPPSSETQE
S47 EKASGPPSSETQEEF
S48 KASGPPSSETQEEFV
T50 SGPPSSETQEEFVDD
A140 EANGLQTAHARAAsP
- gap
A145 QTAHARAAsPLSTAA
S146-p TAHARAAsPLSTAAA
S149 ARAAsPLSTAAATMV
T150 RAAsPLSTAAATMVS
S157 TAAATMVSSSPATPS
S159 AATMVSSSPATPSNI
A161 TMVSSSPATPSNIPQ
T162 MVSSSPATPSNIPQK
S171 SNIPQKPSQPVAKET
S179 QPVAKETSATPQISN
T181 VAKETSATPQISNLT
S185 TSATPQISNLTKTAs
T190 QISNLTKTAsEsIsN
S192-p SNLTKTAsEsIsNLs
S194-p LTKTAsEsIsNLsEA
S196-p KTAsEsIsNLsEAGs
S199-p sEsIsNLsEAGsVKK
S203-p sNLsEAGsVKKGERE
Y261 GAVAGTRYFQCQPKY
Y268 YFQCQPKYGLFAPVH
T278 FAPVHKVTKIGFPST
S284 VTKIGFPSTtPAKAK
T285 TKIGFPSTtPAKAKA
T286-p KIGFPSTtPAKAKAA
S305 VMATTPASLKRsPsA
S309-p TPASLKRsPsAssLS
S311-p ASLKRsPsAssLSSM
S313-p LKRsPsAssLSSMsS
S314-p KRsPsAssLSSMsSV
S316 sPsAssLSSMsSVAs
S319-p AssLSSMsSVAsSVs
S323-p SSMsSVAsSVssKPs
S324 SMsSVAsSVssKPsR
S326-p sSVAsSVssKPsRTG
S327-p SVAsSVssKPsRTGL
S330-p sSVssKPsRTGLLTE
T332 VssKPsRTGLLTETS
S347-p SRYARKIsGTTALQE
T349 YARKIsGTTALQEAL
T350 ARKIsGTTALQEALK
T414 AKMDQLRTMVEAADR
S498 VATVSEKSRIMELEK
S595 KENESLRSKLDHANK
T707 AVKARLDTAEDQHLV
M719 HLVEMEEMLSKLQEA
- gap
- gap
- gap
- gap
- gap
- gap
- under review  
- under review  
- under review  
K794 EREDQLVKAKEKLEN
S830 ELRLKERSVEELQLK
K880 EKKELENKLLELEKK
L882 KELENKLLELEKKME
K886 NKLLELEKKMETSHY
R939 KAAQEANRDLMQDME
K949 MQDMEELKSQADKAK
T960 DKAKAAQTAEDAMQI
T998 KLQSELDTLKENNLK
S1013 TVEELNKSKELLNEE
S1042 KQAAAQKSQQLSALQ
S1110 DADEEKASLQKSISL
S1114 EKASLQKSISLTSAL
S1116 ASLQKSISLTSALLT
S1119 QKSISLTSALLTEKD
T1123 SLTSALLTEKDAELE
S1182 KENKRQLSSSSGNTD
S1183 ENKRQLSSSSGNTDV
S1184 NKRQLSSSSGNTDVQ
S1185 KRQLSSSSGNTDVQT
T1188 LSSSSGNTDVQTEED
V1190 SSSGNTDVQTEEDER
S1246-p EDPNSYDsDDQEKQS
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