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Protein Page:
CDK1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
CDK1 a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Protein kinase, CMGC; Kinase, protein; EC 2.7.11.22; EC 2.7.11.23; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily
Cellular Component: nucleoplasm; centrosome; mitochondrion; spindle microtubule; midbody; nucleus; cytosol
Molecular Function: RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity
Biological Process: regulation of Schwann cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; response to toxin; ventricular cardiac muscle cell development; regulation of embryonic development; stress-activated MAPK cascade; centrosome cycle; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; protein complex assembly; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; mitosis; organ regeneration; fibroblast growth factor receptor signaling pathway; positive regulation of protein import into nucleus, translocation; cell aging; pronuclear fusion; toll-like receptor 2 signaling pathway; chromosome condensation; response to ethanol; toll-like receptor 9 signaling pathway; response to activity; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; response to amine stimulus; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; axon guidance; apoptosis; mitotic nuclear envelope disassembly; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; epithelial cell differentiation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; response to axon injury; DNA replication; epidermal growth factor receptor signaling pathway; cell migration; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; microtubule cytoskeleton organization and biogenesis; DNA repair; G1/S-specific transcription in mitotic cell cycle; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; response to copper ion; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; mitotic cell cycle; positive regulation of DNA replication
Reference #:  P06493 (UniProtKB)
Alt. Names/Synonyms: CDC2; CDC28A; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2; cell division cycle 2, G1 to S and G2 to M; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; p34 protein kinase; P34CDC2
Gene Symbols: CDK1
Molecular weight: 34,095 Da
Basal Isoelectric point: 8.38  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  ErbB/HER Signaling  |  G2/M DNA Damage Checkpoint  |  Microtubule Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CDK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, inhibited: Y15‑p
cell cycle regulation: Y4‑p, T14‑p, Y15‑p, S39‑p, T161‑p
cell growth, altered: T161‑p
enzymatic activity, altered: Y15‑p
enzymatic activity, induced: T161‑p
enzymatic activity, inhibited: T14‑p, Y15‑p
molecular association, regulation: Y15‑p, T161‑p
protein degradation: Y4‑p
ubiquitination: Y4‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 5 Y4-p ____MEDytkIEkIG
0 2 T5-p ___MEDytkIEkIGE
0 1 K6-a __MEDytkIEkIGEG
0 11 K9-u EDytkIEkIGEGtyG
19 1708 T14-p IEkIGEGtyGVVykG
129 3930 Y15-p EkIGEGtyGVVykGR
0 1972 Y19-p EGtyGVVykGRHkTT
0 33 K20-u GtyGVVykGRHkTTG
0 12 K24-u VVykGRHkTTGQVVA
0 129 K33-a TGQVVAMkkIRLEsE
0 17 K33-u TGQVVAMkkIRLEsE
0 6 K34-a GQVVAMkkIRLEsEE
0 2 K34-u GQVVAMkkIRLEsEE
2 18 S39-p MkkIRLEsEEEGVPs
0 1 S46-p sEEEGVPsTAIREIS
0 26 K56-u IREISLLkELRHPNI
0 1 Y77-p LMQDSRLyLIFEFLS
0 1 K89-u FLSMDLKkYLDSIPP
0 4 K130-u RVLHRDLkPQNLLID
0 36 K139-u QNLLIDDkGtIkLAD
0 3 T141-p LLIDDkGtIkLADFG
0 16 K143-u IDDkGtIkLADFGLA
0 11 Y160-p FGIPIRVytHEVVTL
19 104 T161-p GIPIRVytHEVVTLW
0 1 S178-p SPEVLLGsARYSTPV
0 18 K201-u FAELATKkPLFHGDs
1 1 S208-p kPLFHGDsEIDQLFR
0 3 T222-p RIFRALGtPNNEVWP
0 8 K238-u VESLQDYkNTFPKWk
0 4 K245-u kNTFPKWkPGsLASH
0 1 S248-p FPKWkPGsLASHVkN
0 9 K254-u GsLASHVkNLDENGL
0 6 K266-u NGLDLLSkMLIyDPA
0 5 Y270-p LLSkMLIyDPAkRIS
0 16 K274-u MLIyDPAkRISGkMA
1 0 S277 yDPAkRISGkMALNH
0 11 K279-u PAkRISGkMALNHPY
0 6 K295-u NDLDNQIkkM_____
0 4 K296-u DLDNQIkkM______
2543 : Phospho-cdc2 (Thr14) Antibody
4539 : Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb
9111 : Phospho-cdc2 (Tyr15) Antibody
9114 : Phospho-cdc2 (Thr161) Antibody
  mouse

 
Y4 ____MEDYIKIEKIG
I5 ___MEDYIKIEKIGE
K6 __MEDYIKIEKIGEG
K9 EDYIKIEKIGEGtyG
T14-p IEKIGEGtyGVVyKG
Y15-p EKIGEGtyGVVyKGR
Y19-p EGtyGVVyKGRHRVT
K20 GtyGVVyKGRHRVTG
R24 VVyKGRHRVTGQIVA
K33-a TGQIVAMkKIRLESE
K33 TGQIVAMKKIRLESE
K34 GQIVAMkKIRLESEE
K34 GQIVAMkKIRLESEE
S39 MkKIRLESEEEGVPS
S46 SEEEGVPSTAIREIS
K56-u IREISLLkELRHPNI
Y77 LMQDSRLYLIFEFLS
K89 FLSMDLKKYLDSIPP
K130 RVLHRDLKPQNLLID
K139-u QNLLIDDkGtIKLAD
T141-p LLIDDkGtIKLADFG
K143 IDDkGtIKLADFGLA
Y160-p FGIPIRVytHEVVTL
T161-p GIPIRVytHEVVTLW
S178 SPEVLLGSARYSTPV
K201-u FAELATKkPLFHGDS
S208 kPLFHGDSEIDQLFR
T222 RIFRALGTPNNEVWP
K238 VESLQDYKNTFPKWK
K245 KNTFPKWKPGSLASH
S248 FPKWKPGSLASHVKN
K254 GSLASHVKNLDENGL
K266 NGLDLLSKMLVYDPA
Y270 LLSKMLVYDPAKRIS
K274 MLVYDPAKRISGKMA
S277 YDPAKRISGKMALKH
K279 PAKRISGKMALKHPY
K295-u DDLDNQIkkM_____
K296-u DLDNQIkkM______
2543 : Phospho-cdc2 (Thr14) Antibody
4539 : Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb
9111 : Phospho-cdc2 (Tyr15) Antibody
9114 : Phospho-cdc2 (Thr161) Antibody
  rat

 
Y4 ____MEDYIKIEKIG
I5 ___MEDYIKIEKIGE
K6 __MEDYIKIEKIGEG
K9 EDYIKIEKIGEGtyG
T14-p IEKIGEGtyGVVyKG
Y15-p EKIGEGtyGVVyKGR
Y19-p EGtyGVVyKGRHRTT
K20 GtyGVVyKGRHRTTG
R24 VVyKGRHRTTGQIVA
K33 TGQIVAMKKIRLESE
K33 TGQIVAMKKIRLESE
K34 GQIVAMKKIRLESEE
K34 GQIVAMKKIRLESEE
S39 MKKIRLESEEEGVPS
S46 SEEEGVPSTAIREIS
K56 IREISLLKELRHPNI
Y77 LMQDSRLYLIFEFLS
K89 FLSMDLKKYLDSIPP
K130 RVLHRDLKPQNLLID
K139 QNLLIDDKGTIKLAD
T141 LLIDDKGTIKLADFG
K143 IDDKGTIKLADFGLA
Y160 FGIPIRVYTHEVVTL
T161 GIPIRVYTHEVVTLW
S178 SPEVLLGSARYSTPV
K201 FAELATKKPLFHGDS
S208 KPLFHGDSEIDQLFR
T222 RIFRALGTPNNEVWP
K238 VESLQDYKNTFPKWK
K245 KNTFPKWKPGSLASH
S248 FPKWKPGSLASHVKN
K254 GSLASHVKNLDENGL
K266 NGLDLLSKMLVYDPA
Y270 LLSKMLVYDPAKRIS
K274 MLVYDPAKRISGKMA
S277 YDPAKRISGKMALKH
K279 PAKRISGKMALKHPY
K295 DDLDNQIKKM_____
K296 DLDNQIKKM______
2543 : Phospho-cdc2 (Thr14) Antibody
4539 : Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb
  chicken

 
Y4 ____MEDYTKIEKIG
T5 ___MEDYTKIEKIGE
K6 __MEDYTKIEKIGEG
K9 EDYTKIEKIGEGtyG
T14-p IEKIGEGtyGVVYKG
Y15-p EKIGEGtyGVVYKGR
Y19 EGtyGVVYKGRHKTT
K20 GtyGVVYKGRHKTTG
K24 VVYKGRHKTTGQVVA
K33 TGQVVAMKKIRLESE
K33 TGQVVAMKKIRLESE
K34 GQVVAMKKIRLESEE
K34 GQVVAMKKIRLESEE
S39 MKKIRLESEEEGVPS
S46 SEEEGVPSTAIREIS
K56 IREISLLKELHHPNI
Y77 LMQDARLYLIFEFLS
K89 FLSMDLKKYLDTIPS
K130 RVLHRDLKPQNLLID
K139 QNLLIDDKGVIKLAD
V141 LLIDDKGVIKLADFG
K143 IDDKGVIKLADFGLA
Y160 FGIPVRVYTHEVVTL
T161 GIPVRVYTHEVVTLW
S178 SPEVLLGSALYSTPV
K201 FAELATKKPLFHGDS
S208 KPLFHGDSEIDQLFR
T222 RIFRALGTPNNDVWP
K238 VESLQDYKNTFPKWK
K245 KNTFPKWKPGSLGTH
S248 FPKWKPGSLGTHVQN
Q254 GSLGTHVQNLDEDGL
K266 DGLDLLSKMLIYDPA
Y270 LLSKMLIYDPAKRIs
K274 MLIYDPAKRIsGKMA
S277-p YDPAKRIsGKMALNH
K279 PAKRIsGKMALNHPY
K301 TLPANLIKKF_____
K302 LPANLIKKF______
  fruit fly

 
F4 ____MEDFEKIEKIG
E5 ___MEDFEKIEKIGE
K6 __MEDFEKIEKIGEG
K9 EDFEKIEKIGEGTYG
T14 IEKIGEGTYGVVYKG
Y15 EKIGEGTYGVVYKGR
Y19 EGTYGVVYKGRNRLT
K20 GTYGVVYKGRNRLTG
R24 VVYKGRNRLTGQIVA
K33 TGQIVAMKKIRLESD
K33 TGQIVAMKKIRLESD
K34 GQIVAMKKIRLESDD
K34 GQIVAMKKIRLESDD
S39 MKKIRLESDDEGVPS
S46 SDDEGVPSTAIREIS
K56 IREISLLKELKHENI
Y77 LMEENRIYLIFEFLS
K89 FLSMDLKKYMDSLPV
K130 RVLHRDLKPQNLLID
S139 QNLLIDKSGLIKVAD
L141 LLIDKSGLIKVADFG
K143 IDKSGLIKVADFGLG
Y160 FGIPVRIYTHEIVTL
T161 GIPVRIYTHEIVTLW
S178 APEVLLGSPRYSCPV
K201 FAEMATRKPLFQGDS
S208 KPLFQGDSEIDQLFR
T222 RMFRILKTPTEDIWP
K238 VTSLPDYKNTFPCWS
S245 KNTFPCWSTNQLTNQ
Q248 FPCWSTNQLTNQLKN
K254 NQLTNQLKNLDANGI
K266 NGIDLIQKMLIYDPV
Y270 LIQKMLIYDPVHRIS
H274 MLIYDPVHRISAKDI
S277 YDPVHRISAKDILEH
K279 PVHRISAKDILEHPY
R296 GFQSGLVRN______
N297 FQSGLVRN_______
2543 : Phospho-cdc2 (Thr14) Antibody
9111 : Phospho-cdc2 (Tyr15) Antibody
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