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Protein Page:
NF1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NF1 a Ras-GAP, highly expressed in developing neural cells. Possesses tumor suppressor activity, presumably by virtue of its GTPase activating domain. Neurofibromin is phosphorylated in response to EGF in CNS cells and cell lines. Defects in NF1 are the cause of type 1 neurofibromatosis (NF1), Watson syndrome, and familial spinal neurofibromatosis. NF1 is one of the most frequent autosomal dominant diseases. Four alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: GAPs, Ras; GAPs; Tumor suppressor; Motility/polarity/chemotaxis; Nucleolus
Cellular Component: membrane; axon; dendrite; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; phosphatidylethanolamine binding; phosphatidylcholine binding; Ras GTPase activator activity
Biological Process: negative regulation of Rac protein signal transduction; neural tube development; negative regulation of MAP kinase activity; Schwann cell development; extracellular matrix organization and biogenesis; collagen fibril organization; positive regulation of apoptosis; heart development; negative regulation of transcription factor import into nucleus; regulation of Ras GTPase activity; negative regulation of neuroblast proliferation; regulation of glial cell differentiation; positive regulation of adenylate cyclase activity; negative regulation of neurotransmitter secretion; skeletal muscle development; adrenal gland development; forebrain morphogenesis; negative regulation of cell-matrix adhesion; regulation of synaptic transmission, GABAergic; myelination in the peripheral nervous system; camera-type eye morphogenesis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; negative regulation of endothelial cell proliferation; negative regulation of protein kinase activity; positive regulation of endothelial cell proliferation; cerebral cortex development; actin cytoskeleton organization and biogenesis; metanephros development; forebrain astrocyte development; regulation of long-term neuronal synaptic plasticity; negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; wound healing; regulation of cell-matrix adhesion; regulation of blood vessel endothelial cell migration; smooth muscle development; sympathetic nervous system development; positive regulation of Ras GTPase activity; positive regulation of neuron apoptosis; visual learning; negative regulation of Ras protein signal transduction; cell communication; negative regulation of cell migration; regulation of bone resorption; negative regulation of MAPKKK cascade; MAPKKK cascade; liver development; regulation of angiogenesis; peripheral nervous system development; negative regulation of oligodendrocyte differentiation; osteoblast differentiation; negative regulation of angiogenesis; pigmentation; spinal cord development; negative regulation of astrocyte differentiation; Ras protein signal transduction; response to hypoxia; artery morphogenesis; brain development; cognition
Reference #:  P21359 (UniProtKB)
Alt. Names/Synonyms: DKFZp686J1293; FLJ21220; neurofibromatosis-related protein NF-1; neurofibromin; neurofibromin 1; neurofibromin truncated; NF1; NFNS; VRNF; WSS
Gene Symbols: NF1
Molecular weight: 319,372 Da
Basal Isoelectric point: 7.1  Predict pI for various phosphorylation states
Select Structure to View Below

NF1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 Y49 CLINISKYKFSLVIS
0 1 S139 AELRNSASGVLFSLS
0 1 S155 NNFNAVFSRISTRLQ
0 1 S158 NAVFSRISTRLQELT
0 1 T159 AVFSRISTRLQELTV
0 1 K362 NLLFNPSKPFSRGSQ
0 1 S465-p PAIRMAPsLtFKEKV
0 1 T467-p IRMAPsLtFKEKVTS
0 2 K471 PsLtFKEKVTSLKFK
0 2 K476 KEKVTSLKFKEKPTD
0 1 K480 TSLKFKEKPTDLETR
0 1 - gap
0 1 S657-p LLRTPGAsLRKGKGN
0 1 S674-p MDSAAGCsGtPPICR
0 2 T676-p SAAGCsGtPPICRQA
0 1 S858 CLQQRSNSGLATysP
0 13 Y863-p SNSGLATysPPMGPV
0 18 S864-p NSGLATysPPMGPVS
0 6 S876-p PVSERKGsMIsVMsS
0 1 S879-p ERKGsMIsVMsSEGN
0 3 S882-p GsMIsVMsSEGNADT
0 1 S883 sMIsVMsSEGNADTP
0 1 T889 sSEGNADTPVSKFMD
0 1 T915-p KVGLQIRtNVKDLVG
0 2 S926-p DLVGLELsPALyPML
0 1 Y930-p LELsPALyPMLFNKL
0 1 Y1169 MHSIGLGYHKDLQTR
0 1 T1175 GYHKDLQTRATFMEV
0 1 R1204-m1 AETVLADrFERLVEL
0 1 K1283-ac RGNSLASkIMTFCFK
0 1 S1401 SQRFPQNSIGAVGSA
0 1 S1407 NSIGAVGSAMFLRFI
0 1 K1517-ub LLWNNQEkIGQyLSS
0 15 Y1521-p NQEkIGQyLSSNRDH
0 1 K1538 VGRRPFDKMATLLAY
0 1 K1552 YLGPPEHKPVADTHW
0 1 S1567-p SSLNLTSsKFEEFMT
0 1 K1581-ac TRHQVHEkEEFKALK
0 1 T1751-p LKLAHKDtKVSIKVG
0 9 S2188-p SYRDRSFsPGSyERE
0 1 Y2192-p RSFsPGSyERETFAL
0 1 T2444-p AYLAALLtVsEEVRs
0 1 S2446-p LAALLtVsEEVRsRC
0 1 S2451-p tVsEEVRsRCSLKHR
0 2 S2460-p CSLKHRKsLLLTDIS
0 2 S2467 sLLLTDISMENVPMD
0 1 Y2476-p ENVPMDTyPIHHGDP
0 2 T2491-p SYRTLKEtQPWSsPK
0 2 S2496-p KEtQPWSsPKGsEGy
0 2 S2500-p PWSsPKGsEGyLAAT
0 2 Y2503-p sPKGsEGyLAATyPT
0 3 Y2508-p EGyLAATyPTVGQts
0 7 T2514-p TyPTVGQtsPRARKs
0 34 S2515-p yPTVGQtsPRARKsM
0 6 S2521-p tsPRARKsMsLDMGQ
0 6 S2523-p PRARKsMsLDMGQPS
0 35 S2543-p KLLGTRKsFDHLISD
0 1 S2549 KsFDHLISDTKAPKR
0 4 S2561-p PKRQEMEsGIttPPK
0 2 T2564-p QEMEsGIttPPKMRR
0 27 T2565-p EMEsGIttPPKMRRV
1 0 T2575 KMRRVAETDyEMEtQ
0 102 Y2577-p RRVAETDyEMEtQRI
0 1 T2581-p ETDyEMEtQRISSSQ
0 1 S2586 MEtQRISSSQQHPHL
1 20 S2597-p HPHLRKVsVsEsNVL
1 2 S2599-p HLRKVsVsEsNVLLD
1 0 S2601-p RKVsVsEsNVLLDEE
0 2 S2802-p DKENVELsPTTGHCN
0 52 S2817-p SGRTRHGsAsQVQKQ
0 3 S2819-p RTRHGsAsQVQKQRS
0 12 S2829-p QKQRSAGsFKRNsIK
1 12 S2834-p AGsFKRNsIKKIV__
  NF1 iso2  
Y49 CLINISKYKFSLVIS
S139 AELRNSASGVLFSLS
S155 NNFNAVFSRISTRLQ
S158 NAVFSRISTRLQELT
T159 AVFSRISTRLQELTV
K362 NLLFNPSKPFSRGSQ
S465 PAIRMAPSLTFKEKV
T467 IRMAPSLTFKEKVTS
K471 PSLTFKEKVTSLKFK
K476 KEKVTSLKFKEKPTD
K480 TSLKFKEKPTDLETR
- gap
S657 LLRTPGASLRKGKGN
S674 MDSAAGCSGTPPICR
T676 SAAGCSGTPPICRQA
S858 CLQQRSNSGLATYSP
Y863 SNSGLATYSPPMGPV
S864 NSGLATYSPPMGPVS
S876 PVSERKGSMISVMSS
S879 ERKGSMISVMSSEGN
S882 GSMISVMSSEGNADT
S883 SMISVMSSEGNADTP
T889 SSEGNADTPVSKFMD
T915 KVGLQIRTNVKDLVG
S926 DLVGLELSPALYPML
Y930 LELSPALYPMLFNKL
Y1169 MHSIGLGYHKDLQTR
T1175 GYHKDLQTRATFMEV
R1204 AETVLADRFERLVEL
K1283 RGNSLASKIMTFCFK
S1380 SQRFPQNSIGAVGSA
S1386 NSIGAVGSAMFLRFI
K1496 LLWNNQEKIGQYLSS
Y1500 NQEKIGQYLSSNRDH
K1517 VGRRPFDKMATLLAY
K1531 YLGPPEHKPVADTHW
S1546 SSLNLTSSKFEEFMT
K1560 TRHQVHEKEEFKALK
T1730 LKLAHKDTKVSIKVG
S2167-p SYRDRSFsPGSYERE
Y2171 RSFsPGSYERETFAL
T2423 AYLAALLTVSEEVRS
S2425 LAALLTVSEEVRSRC
S2430 TVSEEVRSRCSLKHR
S2439 CSLKHRKSLLLTDIS
S2446 SLLLTDISMENVPMD
Y2455 ENVPMDTYPIHHGDP
T2470 SYRTLKETQPWSSPK
S2475 KETQPWSSPKGSEGY
S2479 PWSSPKGSEGYLAAT
Y2482 SPKGSEGYLAATYPT
Y2487 EGYLAATYPTVGQTS
T2493 TYPTVGQTSPRARKS
S2494 YPTVGQTSPRARKSM
S2500 TSPRARKSMSLDMGQ
S2502 PRARKSMSLDMGQPS
S2522 KLLGTRKSFDHLISD
S2528 KSFDHLISDTKAPKR
S2540 PKRQEMESGITtPPK
T2543 QEMESGITtPPKMRR
T2544-p EMESGITtPPKMRRV
T2554-p KMRRVAEtDYEMETQ
Y2556 RRVAEtDYEMETQRI
T2560 EtDYEMETQRISSSQ
S2565 METQRISSSQQHPHL
S2576-p HPHLRKVsVsEsNVL
S2578-p HLRKVsVsEsNVLLD
S2580-p RKVsVsEsNVLLDEE
S2781 DKENVELSPTTGHCN
S2796-p SGRTRHGsASQVQKQ
S2798 RTRHGsASQVQKQRS
S2808 QKQRSAGSFKRNsIK
S2813-p AGSFKRNsIKKIV__
  NF1 iso5  
Y49 CLINISKYKFSLVIS
S139 AELRNSASGVLFSLS
S155 NNFNAVFSRISTRLQ
S158 NAVFSRISTRLQELT
T159 AVFSRISTRLQELTV
K362 NLLFNPSKPFSRGSQ
S465 PAIRMAPSLTFKEKV
T467 IRMAPSLTFKEKVTS
K471 PSLTFKEKVTSLKFK
K476 KEKVTSLKFKEKPTD
K480 TSLKFKEKPTDLETR
S593-p KFYFVFLs_______
- gap
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- gap
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- gap
- gap
- gap
- gap
- gap
- gap
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- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
Y49-p CLINISKyKFSLVIS
S139-p AELRNSAsGVLFSLS
S155-p NNFNAVFsRIstRLQ
S158-p NAVFsRIstRLQELT
T159-p AVFsRIstRLQELTV
K362-ub NLLFNPSkPFSRGSQ
S465 PAIRMAPSLTFKEkV
T467 IRMAPSLTFKEkVTS
K471-ac PSLTFKEkVTSLkFK
K476-ac KEkVTSLkFKEkPTD
K480-ub TSLkFKEkPTDLETR
- gap
S659 RACTPGASLRKGRGN
S676 MDSTAGCSGTPPICR
T678 STAGCSGTPPICRQA
S860-p CLQQRSSsGLATYsP
Y865 SSsGLATYsPPMGAV
S866-p SsGLATYsPPMGAVS
S878-p AVSERKGsMISVMsS
S881 ERKGsMISVMsSEGN
S884-p GsMISVMsSEGNIDS
S885 sMISVMsSEGNIDSP
S891 sSEGNIDSPVSRFMD
T917 KVGLQIRTNVKDLVG
S928 DLVGLELSPALYPML
Y932 LELSPALYPMLFNKL
Y1171-p MHSIGLGyHKDLQtR
T1177-p GyHKDLQtRATFMEV
R1206 AETVLADRFERLVEL
K1285 RGNSLASKIMTFCFK
S1403-p SQRFPQNsIGAVGsA
S1409-p NsIGAVGsAMFLRFI
K1519 LLWNNQEKIGQYLSS
Y1523 NQEKIGQYLSSNRDH
K1540-ac VGRRPFDkMATLLAY
K1554-ac YLGPPEHkPVADTHW
S1569 SSLNLTSSKFEEFMT
K1583 TRHQVHEKEEFKALK
T1753 LKLAHKDTKVSIKVG
S2190-p SYRDRSFsPGSYERE
Y2194 RSFsPGSYERETFAL
T2446 AYLAALLTVSEEVRS
S2448 LAALLTVSEEVRSRC
S2453 TVSEEVRSRCSLKHR
S2462-p CSLKHRKsLLLTDIs
S2469-p sLLLTDIsMENVPMD
Y2478 ENVPMDTYPIHHGDP
T2493-p SYRTLKEtQPWSSPK
S2498 KEtQPWSSPKGsEGy
S2502-p PWSSPKGsEGyLAAT
Y2505-p SPKGsEGyLAATyPA
Y2510-p EGyLAATyPAVGQts
T2516-p TyPAVGQtsPRARKs
S2517-p yPAVGQtsPRARKsM
S2523-p tsPRARKsMsLDMGQ
S2525-p PRARKsMsLDMGQPS
S2545-p KLLGTRKsFDHLIsD
S2551-p KsFDHLIsDTKAPKR
S2563-p PKRQEMEsGIttPPK
T2566-p QEMEsGIttPPKMRR
T2567-p EMEsGIttPPKMRRV
T2577 KMRRVAETDYEMETQ
Y2579 RRVAETDYEMETQRI
T2583 ETDYEMETQRIPsSQ
S2588-p METQRIPsSQQHPHL
S2599-p HPHLRKVsVsEsNVL
S2601-p HLRKVsVsEsNVLLD
S2603-p RKVsVsEsNVLLDEE
S2804-p DKENVELsPTAGHCN
S2819-p SGRTRHGsAsQVQKQ
S2821-p RTRHGsAsQVQKQRS
S2831-p QKQRSAGsFKRNsIK
S2836-p AGsFKRNsIKKIV__
  rat

 
Y49 CLINISKYKFSLVIS
S139 AELRNSASGVLFSLS
S155 NNFNAVFSRISTRLQ
S158 NAVFSRISTRLQELT
T159 AVFSRISTRLQELTV
K362 NLLFNPSKPFSRGSQ
S465 PALRMAPSLTFKEKV
T467 LRMAPSLTFKEKVTS
K471 PSLTFKEKVTSLKFK
K476 KEKVTSLKFKEKPTD
K480 TSLKFKEKPTDLEAR
- gap
S659 RTCAPGASLRKGRGN
S676 MDSTAGCSGTPPICR
T678 STAGCSGTPPICRQA
S860 CLQQRSSSGLATYSP
Y865 SSSGLATYSPPMGPV
S866 SSGLATYSPPMGPVS
S878-p PVSERKGsMISVMss
S881 ERKGsMISVMssEGN
S884-p GsMISVMssEGNVDs
S885-p sMISVMssEGNVDsP
S891-p ssEGNVDsPVSRFMD
T917 KVGLQIRTNVKDLVG
S928 DLVGLELSPALYPML
Y932 LELSPALYPMLFNKL
Y1171 MHSIGLGYHKDLQTR
T1177 GYHKDLQTRATFMEV
R1206 AETVLADRFERLVEL
K1285 RGNSLASKIMTFCFK
S1382 SQRFPQNSIGAVGSA
S1388 NSIGAVGSAMFLRFI
K1498 LLWNNQEKIGQYLSS
Y1502 NQEKIGQYLSSNRDH
K1519 VGRRPFDKMATLLAY
K1533 YLGPPEHKPVADTHW
S1548 SSLNLTSSKFEEFMT
K1562 TRHQVHEKEEFKALK
T1732 LKLAHKDTKVSIKVG
S2169 SYRDRSFSPGSYERE
Y2173 RSFSPGSYERETFAL
T2425 AYLAALLTVSEEVRS
S2427 LAALLTVSEEVRSRC
S2432 TVSEEVRSRCSLKHR
S2441 CSLKHRKSLLLTDIS
S2448 SLLLTDISMENVPMD
Y2457 ENVPMDTYPIHHGDP
T2472 SSRTLKETQPWSSPR
S2477 KETQPWSSPRGSEGY
S2481 PWSSPRGSEGYLAAT
Y2484 SPRGSEGYLAATYPA
Y2489 EGYLAATYPAVGQTS
T2495 TYPAVGQTSPRARKS
S2496 YPAVGQTSPRARKSM
S2502 TSPRARKSMSLDMGQ
S2504 PRARKSMSLDMGQPS
S2524 KLLGTRKSFDHLISD
S2530 KSFDHLISDTKAPKR
S2542 PKRQEMESGITTPPK
T2545 QEMESGITTPPKMRR
T2546 EMESGITTPPKMRRV
T2556 KMRRVAETDYEMETQ
Y2558 RRVAETDYEMETQRI
T2562 ETDYEMETQRISSSQ
S2567 METQRISSSQQHPHL
S2578-p HPHLRKVsVSESNVL
S2580 HLRKVsVSESNVLLD
S2582 RKVsVSESNVLLDEE
S2783 DKENVELSPTTGHCN
S2798 SGRTRHGSASQVQKQ
S2800 RTRHGSASQVQKQRS
S2810 QKQRSAGSFKRNSIK
S2815 AGSFKRNSIKKIV__
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