Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
calpastatin (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
calpastatin Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Inhibitor protein
Molecular Function: protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity
Reference #:  P20810 (UniProtKB)
Alt. Names/Synonyms: BS-17; Calpain inhibitor; Calpastatin; CAST; heart-type calpastatin; ICAL; MGC9402; Sperm BS-17 component
Gene Symbols: CAST
Molecular weight: 76,573 Da
Basal Isoelectric point: 4.98  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

calpastatin
Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 2 - gap
0 7 - gap
0 1 - gap
0 1 - gap
0 2 - gap
0 1 - gap
0 3 - gap
0 1 - gap
0 1 - gap
0 2 - gap
0 4 - gap
0 1 - gap
0 1 - gap
0 1 - gap
0 1 T42 PEKKSQSTKLSVVHE
0 1 K51-m1 LSVVHEKksQEGkPk
0 1 S52-p SVVHEKksQEGkPkE
0 1 K56-m1 EKksQEGkPkEHTEP
0 1 K58-m1 ksQEGkPkEHTEPKs
0 1 S65-p kEHTEPKsLPKQAsD
0 2 S71-p KsLPKQAsDTGSNDA
0 3 S87 NKKAVSRSAEQQPSE
0 1 P99 PSEKSTEPKTKPQDM
0 5 S133-p DKKKEKKsLtPAVPV
0 4 T135-p KKEKKsLtPAVPVEs
0 15 V138 KKsLtPAVPVEsKPD
0 2 S142-p tPAVPVEsKPDKPSG
0 2 K143 PAVPVEsKPDKPSGK
0 2 D145 VPVEsKPDKPSGKSG
0 1 S148 EsKPDKPSGKSGMDA
0 2 T216 LAKKEGITGPPADSS
0 5 S222 ITGPPADSSKPIGPD
0 2 P228 DSSKPIGPDDAIDAL
0 1 S236 DDAIDALSSDFTCGs
0 1 T240 DALSSDFTCGsPtAA
0 1 G242 LSSDFTCGsPtAAGk
0 10 S243-p SSDFTCGsPtAAGkK
0 3 T245-p DFTCGsPtAAGkKTE
0 2 K249-a GsPtAAGkKTEKEES
0 1 K261 EESTEVLKAQSAGTV
0 1 K313-a ELDLRSIkEVDEAKA
0 5 Y339-p DETIPSEyRLKPATD
0 4 P362 EPEEKPKPRsEsELI
0 25 S364-p EEKPKPRsEsELIDE
0 4 S366-p KPKPRsEsELIDELs
0 3 S373-p sELIDELsEDFDRsE
0 1 S379-p LsEDFDRsECKEKPS
0 1 K384 DRsECKEKPSKPTEK
0 1 K391 KPSKPTEKTEESKAA
0 1 T392 PSKPTEKTEESKAAA
0 1 - gap
0 7 - gap
0 1 K426 PPEPATLKGTVPDDA
0 1 S440-p AVEALADsLGkKEAD
0 2 K443-a ALADsLGkKEADPED
0 2 Y481-p EETIPPDyRLEEVKD
0 1 K500-a PLLPKESkEQLPPMs
0 1 S507-p kEQLPPMsEDFLLDA
0 1 S526 FSGPQNASSLkFEDA
0 1 K529-a PQNASSLkFEDAkLA
0 1 K534-u SLkFEDAkLAAAISE
0 2 S575-p DDALDKLsDsLGQRQ
0 2 S577-p ALDKLsDsLGQRQPD
0 1 K598 MEDKVKEKAKAEHRD
0 1 K606 AKAEHRDKLGERDDt
0 1 T613-p KLGERDDtIPPEyRH
0 2 Y618-p DDtIPPEyRHLLDDN
0 1 K629-a LDDNGQDkPVKPPTK
0 1 T635 DkPVKPPTKKSEDSK
0 1 S655 QDPIDALSGDLDSCP
0 2 K678-u TAKDKCKkAASSSkA
0 1 K684-a KkAASSSkAPkNGGk
0 1 K687-a ASSSkAPkNGGkAKD
0 1 K687 ASSSkAPKNGGkAKD
0 1 K691-a kAPkNGGkAKDSAKT
0 1 K691 kAPkNGGKAKDSAKT
  calpastatin iso5  
S2-p ______MsQPGQKPA
S11-p PGQKPAAsPRPRRAA
K48 KKGSDEKKAAsLGss
K48-m1 KKGSDEKkAAsLGss
S51-p SDEKkAAsLGssQSs
S54-p KkAAsLGssQSsRty
S55-p kAAsLGssQSsRtyA
S58-p sLGssQSsRtyAGGT
T60-p GssQSsRtyAGGTAS
Y61-p ssQSsRtyAGGTASA
S81-p SSGATSKsSsMNPtE
S83-p GATSKsSsMNPtEtK
T87-p KsSsMNPtEtKAVKT
T89-p SsMNPtEtKAVKTEP
T103 PEKKSQSTKLSVVHE
K112 LSVVHEKKSQEGKPK
S113 SVVHEKKSQEGKPKE
K117 EKKSQEGKPKEHTEP
K119 KSQEGKPKEHTEPKS
S126 KEHTEPKSLPKQASD
S132 KSLPKQASDTGSNDA
S148 NKKAVSRSAEQQPSE
P160 PSEKSTEPKTKPQDM
S194 DKKKEKKSLTPAVPV
T196 KKEKKSLTPAVPVES
V199 KKSLTPAVPVESKPD
S203 TPAVPVESKPDKPSG
K204 PAVPVESKPDKPSGK
D206 VPVESKPDKPSGKSG
S209 ESKPDKPSGKSGMDA
- gap
- gap
P276 LLAKPIGPDDAIDAL
S284 DDAIDALSSDFTCGS
T288 DALSSDFTCGSPTAA
G290 LSSDFTCGSPTAAGK
S291 SSDFTCGSPTAAGKK
T293 DFTCGSPTAAGKKTE
K297 GSPTAAGKKTEKEES
K309 EESTEVLKAQSAGTV
K361 ELDLRSIKEVDEAKK
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- under review  
- gap
- gap
- gap
- gap
- gap
- under review  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

► Hide Isoforms 		 
S2 ______MSQPGPKPA
S11-p PGPKPAAsPRPSRGA
K48-a KKGSDEKkAASLGSS
K48 KKGSDEKKAASLGSS
S51 SDEKkAASLGSSQPS
S54 KkAASLGSSQPSRPH
S55 kAASLGSSQPSRPHV
S58 SLGSSQPSRPHVGEA
P60 GSSQPSRPHVGEAAT
H61 SSQPSRPHVGEAATA
S81-p SSAATSKsPSMSTTE
S83 AATSKsPSMSTTETK
T87 KsPSMSTTETKAIPV
T89 PSMSTTETKAIPVNK
S121-p ESKKPQSsEQPVVHE
K130 QPVVHEKKSKGGPKE
S131 PVVHEKKSKGGPKEG
G134 HEKKSKGGPKEGSEP
K136 KKSKGGPKEGSEPKN
N143 KEGSEPKNLPKHTSS
S149 KNLPKHTSSTGSKHA
S165-p KEKALSRsNEQMVSE
S177-p VSEKPSEsKTKFQDV
P214 GKKKEQKPFtPAsPV
T216-p KKEQKPFtPAsPVQS
S219-p QKPFtPAsPVQStPs
S223 tPAsPVQStPsKPsD
T224-p PAsPVQStPsKPsDK
S226-p sPVQStPsKPsDKSG
S229-p QStPsKPsDKSGMDA
T297-p LEKNEGItQPLPDsP
S303-p ItQPLPDsPKPMGtD
T309-p DsPKPMGtDQAIDAL
S317-p DQAIDALsSDFtCss
T321-p DALsSDFtCssPtGK
S323-p LsSDFtCssPtGKQS
S324-p sSDFtCssPtGKQSE
T326-p DFtCssPtGKQSEKE
K328 tCssPtGKQSEKEKS
K341-u KSTGEIFkAQSAGVT
E393 PSHVSQAEQVKEAKA
Y419 EDTVPAEYRLKPAKD
S442-p EPEETSKsLsEsELI
S444-p EETSKsLsEsELIGE
S446-p TSKsLsEsELIGELS
S453 sELIGELSADFDRST
S459 LSADFDRSTYQDkPS
K464-a DRSTYQDkPSTPAEk
K471-a kPSTPAEkkSNDtSQ
K472-a PSTPAEkkSNDtSQt
T476-p AEkkSNDtSQtPPGE
T479-p kSNDtSQtPPGETVP
K504-u PPKLASLkGVVPEDA
S518-p AVETLAGsLGTREAD
T521 TLAGsLGTREADPEH
Y559 EETVPPDYRLEEVKD
Q578 PLLPKESQEQLAPLS
S585 QEQLAPLSDDFLLDA
S604-p FSSPANIsSLEFEDA
E607 PANIsSLEFEDAKLS
K612 SLEFEDAKLSAAISE
S653 DDALDELSDSLGQRP
S655 ALDELSDSLGQRPPD
K676-a LDDKVKEkIKPEHSE
K684-a IKPEHSEkLGERDDT
T691 kLGERDDTIPPEYRH
Y696 DDTIPPEYRHLLDND
K707 LDNDGKDKPEKPPtK
T713-p DKPEKPPtKKTEKPD
S730-p RDPIDALsEDLDSCP
K753 TAKGKSKKTSSSKAS
K758 SKKTSSSKASKDGEK
K761 TSSSKASKDGEKTKD
K761 TSSSKASKDGEKTKD
K765 KASKDGEKTKDSSKK
K765 KASKDGEKTKDSSKK
  calpastatin iso3  
S2 ______MSQPGPKPA
S11 PGPKPAASPRPSRGA
K48 KKGSDEKKAASLGSS
K48 KKGSDEKKAASLGSS
S51 SDEKKAASLGSSQPS
S54 KKAASLGSSQPSRPH
S55 KAASLGSSQPSRPHV
S58 SLGSSQPSRPHVGEA
P60 GSSQPSRPHVGEAAT
H61 SSQPSRPHVGEAATA
S81 SSAATSKSPSMSTTE
S83 AATSKSPSMSTTETK
T87 KSPSMSTTETKAVKT
T89 PSMSTTETKAVKTES
S102 ESKKPQSSEQPVVHE
K111 QPVVHEKKSKGGPKE
S112 PVVHEKKSKGGPKEG
G115 HEKKSKGGPKEGSEP
K117 KKSKGGPKEGSEPKN
N124 KEGSEPKNLPKHTSS
S130 KNLPKHTSSTGSKHA
S146 KEKALSRSNEQMVSE
S158 VSEKPSESKTKFQDV
P195 GKKKEQKPFTPASPV
T197 KKEQKPFTPASPVQS
S200 QKPFTPASPVQSTPS
S204 TPASPVQSTPSKPSD
T205 PASPVQSTPSKPSDK
S207 SPVQSTPSKPSDKSG
S210 QSTPSKPSDKSGMDA
T278 LEKNEGITQPLPDSP
S284 ITQPLPDSPKSTGEI
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K293 KSTGEIFKAQSAGVT
E345 PSHVSQAEQVKEAKA
Y371 EDTVPAEYRLKPAKD
S394 EPEETSKSLSESELI
S396 EETSKSLSESELIGE
S398 TSKSLSESELIGELS
S405 SELIGELSADFDRST
S411 LSADFDRSTYQDKPS
K416 DRSTYQDKPSTPAEK
K423 KPSTPAEKKSNDTSQ
K424 PSTPAEKKSNDTSQT
T428 AEKKSNDTSQTPPGE
T431 KSNDTSQTPPGETVP
K456 PPKLASLKGVVPEDA
S470 AVETLAGSLGTREAD
T473 TLAGSLGTREADPEH
Y511 EETVPPDYRLEEVKD
Q530 PLLPKESQEQLAPLS
S537 QEQLAPLSDDFLLDA
S556 FSSPANISSLEFEDA
E559 PANISSLEFEDAKLS
K564 SLEFEDAKLSAAISE
S605 DDALDELSDSLGQRP
S607 ALDELSDSLGQRPPD
K628 LDDKVKEKIKPEHSE
K636 IKPEHSEKLGERDDT
T643 KLGERDDTIPPEYRH
Y648 DDTIPPEYRHLLDND
K659 LDNDGKDKPEKPPTK
T665 DKPEKPPTKKTEKPD
S682 RDPIDALSEDLDSCP
K705 TAKGKSKKTSSSKAS
K710 SKKTSSSKASKDGEK
K713 TSSSKASKDGEKTKD
K713 TSSSKASKDGEKTKD
K717 KASKDGEKTKDSSKK
K717 KASKDGEKTKDSSKK
  rat

 
S2 ______MSRPGPKPA
S11 PGPKPAASSRPRRGA
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S57-p SSAATGTsPRMSTTG
R59 AATGTsPRMSTTGAK
T63 TsPRMSTTGAKAVKI
A65 PRMSTTGAKAVKIES
S78 ESEKSQSSEPPVIHE
K87 PPVIHEKKPKGKPKE
P88 PVIHEKKPKGKPKEG
K91 HEKKPKGKPKEGSEP
K93 KKPKGKPKEGSEPQT
T100 KEGSEPQTLPKHASD
S106 QTLPKHASDTGSKHA
S122-p KEKALSRsNEQIVSE
S134 VSEKSSESKTKFQDA
S171 VKKKEKKSLtPTLPM
T173-p KKEKKSLtPTLPMES
L176 KKSLtPTLPMESTLN
S180 tPTLPMESTLNKLSD
T181 PTLPMESTLNKLSDK
N183 LPMESTLNKLSDKSG
S186 ESTLNKLSDKSGVNA
T254 LEKNEAITGPLPDsP
S260-p ITGPLPDsPKPMGID
I266 DsPKPMGIDHAIDAL
S274 DHAIDALSSDFTCSs
T278 DALSSDFTCSsPTGK
S280 LSSDFTCSsPTGKQT
S281-p SSDFTCSsPTGKQTE
T283 DFTCSsPTGKQTEKE
K285 TCSsPTGKQTEKEKS
K298 KSTGESSKAQSAGVT
K350 QSHLRQAKQVKEAKA
Y376 EDTVPAEYRLKPAKD
C399 EPEETSKCLSESELI
S401 EETSKCLSESELIGE
S403 TSKCLSESELIGELS
S410 SELIGELSADFVQPT
P416 LSADFVQPTYQEKPS
K421 VQPTYQEKPSMPAAK
K428 KPSMPAAKIKKGVVP
I429 PSMPAAKIKKGVVPD
- gap
- gap
K431 MPAAKIKKGVVPDDA
S445 AVETLARSLGTRKED
T448 TLARSLGTRKEDPED
Y486-p EETIPPDyRLEIVKD
E505 PLLPKEAEEQLPPLS
S512 EEQLPPLSDDFLLDA
L531 FSSPANILSLGFEDA
G534 PANILSLGFEDAKLS
K539 SLGFEDAKLSAAVSE
S580-p DDALDELsDSLGQRQ
S582 ALDELsDSLGQRQPD
K603 LDDKVKEKIKAEHSE
K611 IKAEHSEKLGERDDT
T618 KLGERDDTIPPEYRH
Y623 DDTIPPEYRHLLDND
K634 LDNDGKDKPEKPLTK
T640 DKPEKPLTKNTEKLG
S657 QDPIDALSEDLDSCP
K680-u TTKEKGKkTSSSKAS
K685 GKkTSSSKASkNEEk
K688 TSSSKASKNEEkTKD
K688-u TSSSKASkNEEkTKD
K692 KASkNEEKTKDSSKK
K692-u KASkNEEkTKDSSKK
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.