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Protein Page:
calpastatin (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
calpastatin Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Inhibitor protein
Cellular Component: membrane; cytoplasm
Molecular Function: protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity
Reference #:  P20810 (UniProtKB)
Alt. Names/Synonyms: BS-17; Calpain inhibitor; Calpastatin; CAST; heart-type calpastatin; ICAL; MGC9402; Sperm BS-17 component
Gene Symbols: CAST
Molecular weight: 76,573 Da
Basal Isoelectric point: 4.98  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

calpastatin

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 - gap
0 9 - gap
0 2 - gap
0 4 - gap
0 2 - gap
0 2 - gap
0 5 - gap
0 1 T42 PEKKSQSTKLSVVHE
0 2 S71-p KSLPKQAsDTGSNDA
0 4 S87 NKKAVSRSAEQQPSE
0 1 - gap
0 6 S133-p DKKKEKKsLtPAVPV
0 5 T135-p KKEKKsLtPAVPVEs
0 17 - gap
0 2 S142-p tPAVPVEsKPDKPSG
0 2 - gap
0 2 - gap
0 1 S148 EsKPDKPSGKSGMDA
0 2 T216 LAKKEGITGPPADSS
0 6 S222 ITGPPADSSKPIGPD
0 2 - gap
0 2 S236 DDAIDALSSDFTCGs
0 1 T240 DALSSDFTCGsPtAA
0 1 G242 LSSDFTCGsPtAAGk
0 10 S243-p SSDFTCGsPtAAGkK
0 4 T245-p DFTCGsPtAAGkKTE
0 2 K249-a GsPtAAGkKTEKEES
0 1 K261 EESTEVLKAQSAGTV
0 5 Y339-p DETIPSEyRLKPATD
0 5 P362 EPEEKPKPRsEsELI
0 25 S364-p EEKPKPRsEsELIDE
0 5 S366-p KPKPRsEsELIDELs
0 4 S373-p sELIDELsEDFDRsE
0 1 S379-p LsEDFDRsECKEKPS
0 1 - gap
0 8 - gap
0 1 K426 PPEPATLKGTVPDDA
0 3 S440-p AVEALADsLGkKEAD
0 2 K443-a ALADsLGkKEADPED
0 2 Y481-p EETIPPDyRLEEVKD
0 1 K500-a PLLPKESkEQLPPMS
0 1 S526 FSGPQNASSLkFEDA
0 1 K529-a PQNASSLkFEDAkLA
0 1 K534-u SLkFEDAkLAAAISE
0 1 S563-p PPRDTSQsDKDLDDA
0 3 S575-p DDALDKLsDsLGQRQ
0 3 S577-p ALDKLsDsLGQRQPD
0 2 Y618-p DDTIPPEyRHLLDDN
0 1 K629-a LDDNGQDkPVKPPTK
0 2 K678-u TAKDKCKkAASSSKA
  calpastatin iso5  
S2-p ______MsQPGQKPA
S11-p PGQKPAAsPRPRRAA
S51-p SDEKKAAsLGSsQSs
S55-p KAAsLGSsQSsRTyA
S58-p sLGSsQSsRTyAGGT
Y61-p SsQSsRTyAGGTASA
S81 SSGATSKSSSMNPTE
T103 PEKKSQSTKLSVVHE
S132 KSLPKQASDTGSNDA
S148 NKKAVSRSAEQQPSE
- gap
S194 DKKKEKKSLTPAVPV
T196 KKEKKSLTPAVPVES
- gap
S203 TPAVPVESKPDKPSG
- gap
- gap
S209 ESKPDKPSGKSGMDA
- gap
- gap
- gap
S284 DDAIDALSSDFTCGs
T288 DALSSDFTCGsPTAA
G290 LSSDFTCGsPTAAGK
S291-p SSDFTCGsPTAAGKK
T293 DFTCGsPTAAGKKTE
K297 GsPTAAGKKTEKEES
K309 EESTEVLKAQSAGTV
Y387 DETIPSEYRLKPATD
P410 EPEEKPKPRSESELI
S412 EEKPKPRSESELIDE
S414 KPKPRSESELIDELS
S421 SELIDELSEDFDRSE
S427 LSEDFDRSECKEKPS
- gap
- gap
K474 PPEPATLKGTVPDDA
S488 AVEALADSLGKKEAD
K491 ALADSLGKKEADPED
Y529 EETIPPDYRLEEVKD
K548 PLLPKESKEQLPPMS
S574 FSGPQNASSLKFEDA
K577 PQNASSLKFEDAKLA
K582 SLKFEDAKLAAAISE
S611 PPRDTSQSDKDLDDA
S623 DDALDKLSDSLGQRQ
S625 ALDKLSDSLGQRQPD
Y666 DDTIPPEYRHLLDDN
K677 LDDNGQDKPVKPPTK
K726 TAKDKCKKAASSSKA
  calpastatin iso6  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T42 PEKKSQSTKLSVVHE
S71 KSLPKQASDTGSNDA
S87 NKKAVSRSAEQQPSE
- gap
S133 DKKKEKKSLTPAVPV
T135 KKEKKSLTPAVPVES
- gap
S142 TPAVPVESKPDKPSG
- gap
- gap
S148 ESKPDKPSGKSGMDA
T216 LAKKEGITGPPADSS
S222 ITGPPADSSKPIGPD
- gap
S236 DDAIDALSSDFTCGS
T240 DALSSDFTCGSPTAA
G242 LSSDFTCGSPTAAGK
S243 SSDFTCGSPTAAGKK
T245 DFTCGSPTAAGKKTE
K249 GSPTAAGKKTEKEES
K261 EESTEVLKAQSAGTV
Y339 DETIPSEYRLKPATD
P362 EPEEKPKPRSESELI
S364 EEKPKPRSESELIDE
S366 KPKPRSESELIDELS
S373 SELIDELSEDFDRSE
S379 LSEDFDRSECKEKPS
- gap
- gap
K426 PPEPATLKGTVPDDA
S440 AVEALADSLGKKEAD
K443 ALADSLGKKEADPED
Y481 EETIPPDYRLEEVKD
K500 PLLPKESKEQLPPMS
S526 FSGPQNASSLKFEDA
K529 PQNASSLKFEDAKLA
K534 SLKFEDAKLAAAISE
S563 PPRDTSQSDKDLDDA
S575 DDALDKLSDSLGQRQ
S577 ALDKLSDSLGQRQPD
Y618 DDTIPPEYRHLLDDN
K629 LDDNGQDKPVKPPTK
K678 TAKDKCKKAASSSKA
  mouse

► Hide Isoforms
 
S2 ______MSQPGPKPA
S11-p PGPKPAAsPRPSRGA
S51 SDEKKAASLGSsQPs
S55-p KAASLGSsQPsRPHV
S58-p SLGSsQPsRPHVGEA
H61 SsQPsRPHVGEAATA
S81-p SSAATSKsPSMSTTE
S121-p ESKKPQSsEQPVVHE
S149 KNLPKHTSSTGSKHA
S165-p KEKALSRsNEQMVSE
S177-p VSEKPSEsKTKFQDV
- gap
T216-p KKEQKPFtPAsPVQS
S219-p QKPFtPAsPVQStPs
S223 tPAsPVQStPsKPsD
T224-p PAsPVQStPsKPsDK
S226-p sPVQStPsKPsDKSG
S229-p QStPsKPsDKSGMDA
T297-p LEKNEGItQPLPDsP
S303-p ItQPLPDsPKPMGtD
T309-p DsPKPMGtDQAIDAL
S317-p DQAIDALsSDFtCss
T321-p DALsSDFtCssPtGK
S323-p LsSDFtCssPtGKQS
S324-p sSDFtCssPtGKQSE
T326-p DFtCssPtGKQSEKE
K328 tCssPtGKQSEKEKS
K341-u KSTGEIFkAQSAGVT
Y419 EDTVPAEYRLKPAKD
S442-p EPEETSKsLsEsELI
S444-p EETSKsLsEsELIGE
S446-p TSKsLsEsELIGELS
S453 sELIGELSADFDRST
S459 LSADFDRSTYQDKPS
T476-p AEKKSNDtSQtPPGE
T479-p KSNDtSQtPPGETVP
K504-u PPKLASLkGVVPEDA
S518-p AVETLAGsLGTREAD
T521 TLAGsLGTREADPEH
Y559 EETVPPDYRLEEVKD
Q578 PLLPKESQEQLAPLS
S604-p FSSPANIsSLEFEDA
- gap
K612 SLEFEDAKLSAAISE
- gap
S653 DDALDELSDSLGQRP
S655 ALDELSDSLGQRPPD
Y696 DDTIPPEYRHLLDND
K707 LDNDGKDKPEKPPTK
K753 TAKGKSKKTSSSKAS
  calpastatin iso3  
S2 ______MSQPGPKPA
S11 PGPKPAASPRPSRGA
S51 SDEKKAASLGSSQPS
S55 KAASLGSSQPSRPHV
S58 SLGSSQPSRPHVGEA
H61 SSQPSRPHVGEAATA
S81 SSAATSKSPSMSTTE
S102 ESKKPQSSEQPVVHE
S130 KNLPKHTSSTGSKHA
S146 KEKALSRSNEQMVSE
S158 VSEKPSESKTKFQDV
- gap
T197 KKEQKPFTPASPVQS
S200 QKPFTPASPVQSTPS
S204 TPASPVQSTPSKPSD
T205 PASPVQSTPSKPSDK
S207 SPVQSTPSKPSDKSG
S210 QSTPSKPSDKSGMDA
T278 LEKNEGITQPLPDSP
S284 ITQPLPDSPKSTGEI
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K293 KSTGEIFKAQSAGVT
Y371 EDTVPAEYRLKPAKD
S394 EPEETSKSLSESELI
S396 EETSKSLSESELIGE
S398 TSKSLSESELIGELS
S405 SELIGELSADFDRST
S411 LSADFDRSTYQDKPS
T428 AEKKSNDTSQTPPGE
T431 KSNDTSQTPPGETVP
K456 PPKLASLKGVVPEDA
S470 AVETLAGSLGTREAD
T473 TLAGSLGTREADPEH
Y511 EETVPPDYRLEEVKD
Q530 PLLPKESQEQLAPLS
S556 FSSPANISSLEFEDA
- gap
K564 SLEFEDAKLSAAISE
- gap
S605 DDALDELSDSLGQRP
S607 ALDELSDSLGQRPPD
Y648 DDTIPPEYRHLLDND
K659 LDNDGKDKPEKPPTK
K705 TAKGKSKKTSSSKAS
  rat

 
S2 ______MSRPGPKPA
S11 PGPKPAASSRPRRGA
- gap
- gap
- gap
- gap
S57-p SSAATGTsPRMSTTG
S78 ESEKSQSSEPPVIHE
S106 QTLPKHASDTGSKHA
S122-p KEKALSRsNEQIVSE
S134 VSEKSSESKTKFQDA
S171 VKKKEKKSLtPTLPM
T173-p KKEKKSLtPTLPMES
- gap
S180 tPTLPMESTLNKLSD
T181 PTLPMESTLNKLSDK
- gap
S186 ESTLNKLSDKSGVNA
T254 LEKNEAITGPLPDsP
S260-p ITGPLPDsPKPMGID
- gap
S274-p DHAIDALsSDFTCSs
T278 DALsSDFTCSsPTGK
S280 LsSDFTCSsPTGKQT
S281-p sSDFTCSsPTGKQTE
T283 DFTCSsPTGKQTEKE
K285 TCSsPTGKQTEKEKS
K298 KSTGESSKAQSAGVT
Y376 EDTVPAEYRLKPAKD
C399 EPEETSKCLSESELI
S401 EETSKCLSESELIGE
S403 TSKCLSESELIGELS
S410 SELIGELSADFVQPT
P416 LSADFVQPTYQEKPS
- gap
- gap
K431 MPAAKIKKGVVPDDA
S445 AVETLARSLGTRKED
T448 TLARSLGTRKEDPED
Y486-p EETIPPDyRLEIVKD
- gap
L531 FSSPANILSLGFEDA
- gap
K539 SLGFEDAKLSAAVSE
- gap
S580-p DDALDELsDSLGQRQ
S582 ALDELsDSLGQRQPD
Y623 DDTIPPEYRHLLDND
K634 LDNDGKDKPEKPLTK
K680-u TTKEKGKkTSSSKAS
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