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Protein Page:
BRAF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BRAF a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; EC 2.7.1.37; Kinase, protein; Protein kinase, TKL; TKL group; RAF family
Cellular Component: neuron projection; plasma membrane; cytosol; nucleus
Molecular Function: small GTPase binding; identical protein binding; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; protein heterodimerization activity; mitogen-activated protein kinase kinase binding; calcium ion binding; ATP binding; protein kinase activity
Biological Process: synaptic transmission; response to peptide hormone stimulus; organ morphogenesis; fibroblast growth factor receptor signaling pathway; response to cAMP; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein heterooligomerization; small GTPase mediated signal transduction; MAPKKK cascade; negative regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; negative regulation of apoptosis
Reference #:  P15056 (UniProtKB)
Alt. Names/Synonyms: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-RAF1; BRAF; BRAF1; FLJ95109; MGC126806; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; RAFB1; Serine/threonine-protein kinase B-raf; v-Raf murine sarcoma viral oncogene homolog B1
Gene Symbols: BRAF
Molecular weight: 84,437 Da
Basal Isoelectric point: 7.29  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BRAF

Protein Structure Not Found.


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Sites Implicated In
cell differentiation, altered: T599‑p, S602‑p, S729‑p
cell growth, altered: S151‑p, S365‑p, T401‑p, S429‑p, T599‑p, S602‑p, S729‑p, S750‑p, T753‑p
cell growth, induced: S446‑p
transcription, altered: T599‑p, S602‑p
transcription, induced: S365‑p, S429‑p
enzymatic activity, induced: S446‑p, S579‑p, T599‑p, S602‑p, S729‑p
enzymatic activity, inhibited: S365‑p, T401‑p, S429‑p, T440‑p
molecular association, regulation: S151‑p, T401‑p, S446‑p, T599‑p, S602‑p, S729‑p, S750‑p, T753‑p
protein conformation: S446‑p, T599‑p, S602‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 T119-p SSSASMDtVTSSSSS
0 1 S147-p NPTDVARsNPKsPQK
2 44 S151-p VARsNPKsPQKPIVR
0 9 T244-p FVRKTFFtLAFCDFC
0 1 K253-ac AFCDFCRkLLFQGFR
0 3 S333-p IGPQILTsPsPSKSI
0 3 S335-p PQILTsPsPSKSIPI
0 31 S363-p QFGQRDRsssAPNVH
0 8 S364-p FGQRDRsssAPNVHI
7 42 S365-p GQRDRsssAPNVHIN
1 5 T373-p APNVHINtIEPVNID
0 1 S394-p GFRGDGGsTtGLsAt
0 1 T396-p RGDGGsTtGLsAtPP
0 2 S399-p GGsTtGLsAtPPASL
2 23 T401-p sTtGLsAtPPASLPG
0 1 S409 PPASLPGSLTNVKAL
0 1 K418-ac TNVKALQksPGPQRE
1 32 S419-p NVKALQksPGPQRER
0 8 S428-p GPQRERKsssSsEDR
6 32 S429-p PQRERKsssSsEDRN
0 6 S430-p QRERKsssSsEDRNR
0 3 S431 RERKsssSsEDRNRM
0 5 S432-p ERKsssSsEDRNRMK
2 6 T440-p EDRNRMKtLGRRDss
9 112 S446-p KtLGRRDssDDWEIP
0 141 S447-p tLGRRDssDDWEIPD
1 7 S465-p TVGQRIGsGsFGTVY
1 0 S467-p GQRIGsGsFGTVYKG
1 0 S579-p IIHRDLKsNNIFLHE
8 0 T599-p IGDFGLAtVksRWsG
0 2 K601-ub DFGLAtVksRWsGSH
8 0 S602-p FGLAtVksRWsGSHQ
0 2 S605-p AtVksRWsGSHQFEQ
0 1 S614-p SHQFEQLsGSILWMA
0 1 K687-ub KVRSNCPkAMKRLMA
4 54 S729-p PKIHRSAsEPsLNRA
0 3 S732-p HRSAsEPsLNRAGFQ
0 1 Y746-p QTEDFSLyACAsPKt
1 6 S750-p FSLyACAsPKtPIQA
3 2 T753-p yACAsPKtPIQAGGy
0 1 Y760-p tPIQAGGyGAFPVH_
2696 : Phospho-B-Raf (Ser445) Antibody
  mouse

► Hide Isoforms
 
- gap
N131 TPTDASRNNPKsPQK
S135-p ASRNNPKsPQKPIVR
T228 FVRKTFFTLAFCDFC
K237 AFCDFCRKLLFQGFR
S316-p TGPQILTsPsPSKSI
S318-p PQILTsPsPSKSIPI
S346 QFGQRDRSSsAPNVH
S347 FGQRDRSSsAPNVHI
S348-p GQRDRSSsAPNVHIN
T356 APNVHINTIEPVNID
S429 PGPVFRGSTTGLSAT
T431 PVFRGSTTGLSATPP
S434 RGSTTGLSATPPASL
T436 STTGLSATPPASLPG
S444 PPASLPGSLTNVKAL
K453 TNVKALQKsPGPQRE
S454-p NVKALQKsPGPQRER
S463-p GPQRERKsssssSSE
S464-p PQRERKsssssSSED
S465-p QRERKsssssSSEDR
S466-p RERKsssssSSEDRS
S467-p ERKsssssSSEDRSR
T477 EDRSRMKTLGRRDss
S483-p KTLGRRDssDDWEIP
S484-p TLGRRDssDDWEIPD
S502 TVGQRIGSGSFGTVY
S504 GQRIGSGSFGTVYKG
S616 IIHRDLKSNNIFLHE
T636 IGDFGLATVKSRWsG
K638 DFGLATVKSRWsGSH
S639 FGLATVKSRWsGSHQ
S642-p ATVKSRWsGSHQFEQ
S651 SHQFEQLSGSILWMA
K724 KVRSNCPKAMKRLMA
S766-p PKIHRSAsEPsLNRA
S769-p HRSAsEPsLNRAGFQ
Y783 QTEDFSLYACAsPKt
S787-p FSLYACAsPKtPIQA
T790-p YACAsPKtPIQAGGY
Y797 tPIQAGGYGEFAAFK
2696 : Phospho-B-Raf (Ser445) Antibody
  BRAF iso3  
T53 SSSASMDTVTSSSSS
N81 TPTDASRNNPKSPQK
S85 ASRNNPKSPQKPIVR
T178 FVRKTFFTLAFCDFC
K187 AFCDFCRKLLFQGFR
S266 TGPQILTSPSPSKSI
S268 PQILTSPSPSKSIPI
S296-p QFGQRDRsSsAPNVH
S297 FGQRDRsSsAPNVHI
S298-p GQRDRsSsAPNVHIN
T306 APNVHINTIEPVNID
S327 GFRGDGGSTTGLSAt
T329 RGDGGSTTGLSAtPP
S332 GGSTTGLSAtPPASL
T334-p STTGLSAtPPASLPG
S342-p PPASLPGsLTNVKAL
K351 TNVKALQKSPGPQRE
S352 NVKALQKSPGPQRER
S361 GPQRERKSSSSSSSE
S362 PQRERKSSSSSSSED
S363 QRERKSSSSSSSEDR
S364 RERKSSSSSSSEDRS
S365 ERKSSSSSSSEDRSR
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  rat

 
- gap
N130 TPTDVSRNNPKSPQK
S134 VSRNNPKSPQKPIVR
T227 FVRKTFFTLAFCDFC
K236 AFCDFCRKLLFQGFR
S315 IGPQILTSPSPSKSI
S317 PQILTSPSPSKSIPI
S345 QFGQRDRSSsAPNVH
S346 FGQRDRSSsAPNVHI
S347-p GQRDRSSsAPNVHIN
T355 APNVHINTIEPVNID
S428 PGPVFRGSTTGLSAT
T430 PVFRGSTTGLSATPP
S433 RGSTTGLSATPPASL
T435 STTGLSATPPASLPG
S443 PPASLPGSLTNVKAL
K452 TNVKALQKSPGPQRE
S453 NVKALQKSPGPQRER
S462 GPQRERKSsSSSSST
S463-p PQRERKSsSSSSSTE
S464 QRERKSsSSSSSTED
S465 RERKSsSSSSSTEDR
S466 ERKSsSSSSSTEDRS
T477 EDRSRMKTLGRRDss
S483-p KTLGRRDssDDWEIP
S484-p TLGRRDssDDWEIPD
S502 TVGQRIGSGSFGTVY
S504 GQRIGSGSFGTVYKG
S616 IIHRDLKSNNIFLHE
T636 IGDFGLATVKSRWSG
K638 DFGLATVKSRWSGSH
S639 FGLATVKSRWSGSHQ
S642 ATVKSRWSGSHQFEQ
S651 SHQFEQLSGSILWMA
K724 KVRSNCPKAMKRLMA
S766-p PKIHRSAsEPSLNRA
S769 HRSAsEPSLNRAGFQ
Y783 QTEDFSLYACASPKT
S787 FSLYACASPKTPIQA
T790 YACASPKTPIQAGGY
Y797 TPIQAGGYGGFPVH_
  chicken

 
T119 SSSASTDTVASSSSS
N147 NPTDMSRNNPKSPQK
S151 MSRNNPKSPQKPIVR
T244 FVRKTFFTLAFCDFC
K253 AFCDFCRKLLFQGFR
S333 VGPPILPSPSPSKSI
S335 PPILPSPSPSKSIPI
S363 QFGQRDRSSsAPNVH
S364 FGQRDRSSsAPNVHI
S365-p GQRDRSSsAPNVHIN
T373 APNVHINTIEPVNID
S434 PGPVFRGSTAGLSAT
A436 PVFRGSTAGLSATPP
S439 RGSTAGLSATPPASL
T441 STAGLSATPPASLPG
S449 PPASLPGSLTNVKAL
K458 TNVKALQKSPGPQRE
S459 NVKALQKSPGPQRER
S468 GPQRERKSSSSSEDR
S469 PQRERKSSSSSEDRN
S470 QRERKSSSSSEDRNR
S471 RERKSSSSSEDRNRM
S472 ERKSSSSSEDRNRMK
T480 EDRNRMKTLGRRDsS
S486-p KTLGRRDsSDDWEIP
S487 TLGRRDsSDDWEIPD
S505 TVGQRIGSGSFGTVY
S507 GQRIGSGSFGTVYKG
S619 IIHRDLKSNNIFLHE
T639 IGDFGLATVKSRWSG
K641 DFGLATVKSRWSGSH
S642 FGLATVKSRWSGSHQ
S645 ATVKSRWSGSHQFEQ
S654 SHQFEQLSGSILWMA
K727 KVRSNCPKAMKRLMA
S769-p PKIHRSAsEPSLNRA
S772 HRSAsEPSLNRAGFQ
Y786 QTEDFSLYACASPKt
S790 FSLYACASPKtPIQA
T793-p YACASPKtPIQAGGY
Y800 tPIQAGGYGGFPVH_
  quail

 
T119 SSSASTDTVASSSSS
N147 NPTDMSRNNPKSPQK
S151 MSRNNPKSPQKPIVR
T244 FVRKTFFTLAFCDFC
K253 AFCDFCRKLLFQGFR
S333 VGPPILPSPSPSKSI
S335 PPILPSPSPSKSIPI
S363 QFGQRDRSSSAPNVH
S364 FGQRDRSSSAPNVHI
S365 GQRDRSSSAPNVHIN
T373 APNVHINTIEPVNID
- gap
A436 PVFRGSTAGLSATPP
S439 RGSTAGLSATPPASL
T441 STAGLSATPPASLPG
S449 PPASLPGSLTNVKAL
K458 TNVKALQKSPGPQRE
S459 NVKALQKSPGPQRER
S468 GPQRERKSsSSSEDR
S469-p PQRERKSsSSSEDRN
S470 QRERKSsSSSEDRNR
S471 RERKSsSSSEDRNRM
S472 ERKSsSSSEDRNRMK
T480 EDRNRMKTLGRRDsS
S486-p KTLGRRDsSDDWEIP
S487 TLGRRDsSDDWEIPD
S505 TVGQRIGSGSFGTVY
S507 GQRIGSGSFGTVYKG
S619 IIHRDLKSNNIFLHE
T639 IGDFGLATVKSRWSG
K641 DFGLATVKSRWSGSH
S642 FGLATVKSRWSGSHQ
S645 ATVKSRWSGSHQFEQ
S654 SHQFEQLSGSILWMA
K727 KVRSNCPKAMKRLMA
S769 PKIHRSASEPSLNRA
S772 HRSASEPSLNRAGFQ
Y786 QTEDFSLYACASPKT
S790 FSLYACASPKTPIQA
T793 YACASPKTPIQAGGY
Y800 TPIQAGGYGEFAAFK
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