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Protein Page:
AS160 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
AS160 a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue. Note: This description may include information from UniProtKB.
Protein type: GAPs, Rab; GAPs
Cellular Component: cytoplasmic vesicle membrane; cytoplasm
Molecular Function: Rab GTPase activator activity
Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus; positive regulation of Rab GTPase activity
Reference #:  O60343 (UniProtKB)
Alt. Names/Synonyms: Acrg embryonic lethality minimal region ortholog; Akt substrate of 160 kDa; AS160; DKFZp779C0666; KIAA0603; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC1 domain family member 4; TBC1 domain family, member 4; TBC1D4; TBCD4
Gene Symbols: TBC1D4
Molecular weight: 146,563 Da
Basal Isoelectric point: 6.57  Predict pI for various phosphorylation states
CST Pathways:  Insulin Receptor Signaling  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AS160

Protein Structure Not Found.


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Sites Implicated In
molecular association, regulation: S318‑p, S341‑p, T568‑p, S588‑p, T642‑p, S751‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S66-p MAEIRRRsQKPEAGG
0 1 S102-p PAPGAGAsGGTSPSA
0 1 S230 DDCMEKFSLHEQQRL
0 3 V259 LADLEVVVPGsPGDC
0 18 S262-p LEVVVPGsPGDCLPE
0 1 D272-ca DCLPEEAdGtDTHLG
0 1 T274-p LPEEAdGtDTHLGLP
0 1 - gap
0 3 S285-p LGLPAGAsQPALTSS
0 7 S304-p PERILEDsGFDEQQE
0 6 S314-p DEQQEFRsRCssVtG
0 6 S317-p QEFRsRCssVtGVQR
10 339 S318-p EFRsRCssVtGVQRR
0 8 T320-p RsRCssVtGVQRRVH
8 362 S341-p QPRRRHAsAPsHVQP
0 21 S344-p RRHAsAPsHVQPsDs
0 6 S349-p APsHVQPsDsEKNRt
0 1 S351-p sHVQPsDsEKNRtML
0 1 T356-p sDsEKNRtMLFQVGR
0 3 S370-p RFEINLIsPDTKSVV
0 1 K477-ac GLYPPRAkLVIQRHL
1 9 S485-p LVIQRHLsSLTDNEQ
0 1 T528 LCEAKQKTHVHIGEG
0 1 S540 GEGPSTISNSTIPEN
0 1 S551-p IPENATSsGRFKLDI
0 56 S566-p LKNKAKRsLtssLEN
2 21 T568-p NKAKRsLtssLENIF
0 12 S569-p KAKRsLtssLENIFS
4 100 S570-p AKRsLtssLENIFSR
0 1 R577 sLENIFSRGANRMRG
14 268 S588-p RMRGRLGsVDsFERS
0 45 S591-p GRLGsVDsFERSNsL
0 20 S597-p DsFERSNsLAsEKDY
0 12 S600-p ERSNsLAsEKDYSPG
0 2 S605 LAsEKDYSPGDsPPG
0 10 S609-p KDYSPGDsPPGtPPA
0 6 T613-p PGDsPPGtPPAsPPS
0 5 S617-p PPGtPPAsPPSsAWQ
0 1 S620 tPPAsPPSsAWQTFP
0 1 S621-p PPAsPPSsAWQTFPE
0 1 S633-p FPEEDSDsPQFRRRA
27 145 T642-p QFRRRAHtFsHPPss
0 14 S644-p RRRAHtFsHPPssTK
0 7 S648-p HtFsHPPssTKRKLN
0 7 S649-p tFsHPPssTKRKLNL
5 51 S666-p GRAQGVRsPLLRQSS
0 31 S672 RsPLLRQSSSEQCSN
0 43 S673 sPLLRQSSSEQCSNL
0 15 S674 PLLRQSSSEQCSNLS
0 30 S678 QSSSEQCSNLSSVRR
0 1 T701-p SSLPSLHtSFsAPsF
2 2 S704-p PSLHtSFsAPsFTAP
0 1 S707-p HtSFsAPsFTAPSFL
0 30 T749-p DGEGRKRtsstCsNE
0 41 S750-p GEGRKRtsstCsNEs
10 53 S751-p EGRKRtsstCsNEsL
0 52 T752-p GRKRtsstCsNEsLs
0 129 S754-p KRtsstCsNEsLsVG
0 78 S757-p sstCsNEsLsVGGts
0 13 S759-p tCsNEsLsVGGtsVt
0 4 T763-p EsLsVGGtsVtPRRI
0 1 S764-p sLsVGGtsVtPRRIS
0 16 T766-p sVGGtsVtPRRISWR
0 3 S782-p RIFLRVAsPMNKsPs
0 5 S787-p VAsPMNKsPsAMQQQ
0 1 S789-p sPMNKsPsAMQQQDG
0 1 S806-p RNELLPLsPLsPTME
0 1 S809-p LLPLsPLsPTMEEEP
0 1 S922 LKEGVPKSRRGEIWQ
0 1 S1126 VALSLLSSQETLIME
0 1 Y1191-p DELQESSySCEDSET
0 1 S1207-p EKLERANsQLKRQNM
0 1 S1246-p TRETKMKsLIRTLEQ
8619 : Phospho-AS160 (Ser318) (D3D11) Rabbit mAb
8730 : Phospho-AS160 (Ser588) (D8E4) Rabbit mAb
4288 : Phospho-AS160 (Thr642) Antibody
8881 : Phospho-AS160 (Thr642) (D27E6) Rabbit mAb
  AS160 iso3  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T40 LCEAKQKTHVHIGEG
S52 GEGPSTISNSTIPEN
S63 IPENATSSGRFKLDI
S78 LKNKAKRSLtSsLEN
T80-p NKAKRSLtSsLENIF
S81 KAKRSLtSsLENIFS
S82-p AKRSLtSsLENIFSR
R89 sLENIFSRGANRMRG
S100-p RMRGRLGsVDSFERS
S103 GRLGsVDSFERSNSL
S109 DSFERSNSLASEKDY
S112 ERSNSLASEKDYSPG
S117 LASEKDYSPGDSPPG
S121 KDYSPGDSPPGTPPA
T125 PGDSPPGTPPASPPS
S129 PPGTPPASPPSSAWQ
S132 TPPASPPSSAWQTFP
S133 PPASPPSSAWQTFPE
S145 FPEEDSDSPQFRRRA
T154-p QFRRRAHtFsHPPSS
S156-p RRRAHtFsHPPSSTK
S160 HtFsHPPSSTKRKLN
S161 tFsHPPSSTKRKLNL
S178-p GRAQGVRsPLLRQss
S184-p RsPLLRQsssEQCsD
S185-p sPLLRQsssEQCsDG
S186-p PLLRQsssEQCsDGE
S190-p QsssEQCsDGEGRKR
- gap
- gap
- gap
T198-p DGEGRKRtsstCsNE
S199-p GEGRKRtsstCsNEs
S200-p EGRKRtsstCsNEsL
T201-p GRKRtsstCsNEsLs
S203-p KRtsstCsNEsLsVG
S206-p sstCsNEsLsVGGTS
S208-p tCsNEsLsVGGTSVt
T212 EsLsVGGTSVtPRRI
S213 sLsVGGTSVtPRRIS
T215-p sVGGTSVtPRRISWR
S231 RIFLRVASPMNKSPS
S236 VASPMNKSPSAMQQQ
S238 SPMNKSPSAMQQQDG
S255 RNELLPLSPLSPTME
S258 LLPLSPLSPTMEEEP
- gap
- gap
- gap
- under review  
- gap
8730 : Phospho-AS160 (Ser588) (D8E4) Rabbit mAb
4288 : Phospho-AS160 (Thr642) Antibody
8881 : Phospho-AS160 (Thr642) (D27E6) Rabbit mAb
  mouse

► Hide Isoforms
 
S66 MAEIRRRSQKPDAGG
G102 PAPGAGVGGGAGSGA
S230-p DDCKDKFsLHEQQRL
S258-p MGVLEVEsPVsPDDS
S261-p LEVEsPVsPDDSLPE
D271 DSLPEKADGTVNsPR
T273 LPEKADGTVNsPRAL
S276-p KADGTVNsPRALPSL
S291 ASLPALASQPALASS
C310 PERILEDCGFDEQQE
S320-p DEQQEFRsRCssVTG
S323-p QEFRsRCssVTGVMQ
S324-p EFRsRCssVTGVMQK
T326 RsRCssVTGVMQKKV
S348-p QPRRRHAsAPsHVQP
S351-p RRHAsAPsHVQPSDS
S356 APsHVQPSDSEKNRT
S358 sHVQPSDSEKNRTML
T363 SDSEKNRTMLFQVGR
S377-p RFEINLIsPDTKSVV
K484 GLYPPRAKLVIQRHL
S492-p LVIQRHLsSLTDNEQ
T535 LCEAKQRTHVHIGEG
S547 GEGPAIISNSTIPEN
G558 IPENVTSGGRFKLDV
S573-p LKNKAKRsLtssLEN
T575-p NKAKRsLtssLENIF
S576-p KAKRsLtssLENIFS
S577-p AKRsLtssLENIFSr
R584-m1 sLENIFSrGANRMRG
S595-p RMRGRLGsMDsFERA
S598-p GRLGsMDsFERANsL
S604-p DsFERANsLASEKDF
S607 ERANsLASEKDFsPG
S612-p LASEKDFsPGDsPPG
S616-p KDFsPGDsPPGtPPA
T620-p PGDsPPGtPPAsPLs
S624-p PPGtPPAsPLsSAWH
S627-p tPPAsPLsSAWHAFP
S628 PPAsPLsSAWHAFPE
S640-p FPEEDSDsPQFRRRA
T649-p QFRRRAHtFsHPPSS
S651-p RRRAHtFsHPPSSSR
S655 HtFsHPPSSSRRKLN
S656 tFsHPPSSSRRKLNL
S673-p GKAHGLRsPLLRQSs
S679 RsPLLRQSsSEQCSI
S680-p sPLLRQSsSEQCSIV
S681 PLLRQSsSEQCSIVP
S685 QSsSEQCSIVPSARR
T708 CSLPSLHTSFsAPSF
S711-p PSLHTSFsAPSFTAP
S714 HTSFsAPSFTAPSFL
T756-p DGEGRKRtsstCsNE
S757-p GEGRKRtsstCsNEs
S758-p EGRKRtsstCsNEsL
T759-p GRKRtsstCsNEsLN
S761-p KRtsstCsNEsLNAG
S764-p sstCsNEsLNAGGtP
N766 tCsNEsLNAGGtPVt
T770-p EsLNAGGtPVtPRRV
- gap
T773-p NAGGtPVtPRRVSWR
S789-p RIFLRVAsPVNKsPS
S794-p VAsPVNKsPSAMQQQ
S796 sPVNKsPSAMQQQKD
S814 RTELLPLSPLSPTME
S817 LLPLSPLSPTMEEEP
S931-p LKEGVPKsRRGEIWQ
S1135-p VALSLLSsQEALIME
Y1200 DELLESSYACEDNES
N1216 EKLERANNQLKRQNM
A1255 TRETKMKALIRTLEQ
8619 : Phospho-AS160 (Ser318) (D3D11) Rabbit mAb
8730 : Phospho-AS160 (Ser588) (D8E4) Rabbit mAb
4288 : Phospho-AS160 (Thr642) Antibody
8881 : Phospho-AS160 (Thr642) (D27E6) Rabbit mAb
  AS160 iso2  
S66 MAEIRRRSQKPDAGG
G102 PAPGAGVGGGAGSGA
S230 DDCKDKFSLHEQQRL
S258 MGVLEVESPVSPDDS
S261 LEVESPVSPDDSLPE
D271 DSLPEKADGTVNSPR
T273 LPEKADGTVNSPRAL
S276 KADGTVNSPRALPSL
S291 ASLPALASQPALASS
C310 PERILEDCGFDEQQE
S320 DEQQEFRSRCSSVTG
S323 QEFRSRCSSVTGVMQ
S324 EFRSRCSSVTGVMQK
T326 RSRCSSVTGVMQKKV
S348 QPRRRHASAPSHVQP
S351 RRHASAPSHVQPSDS
S356 APSHVQPSDSEKNRT
S358 SHVQPSDSEKNRTML
T363 SDSEKNRTMLFQVGR
S377 RFEINLISPDTKSVV
K484 GLYPPRAKLVIQRHL
S492 LVIQRHLSSLTDNEQ
T535 LCEAKQRTHVHIGEG
S547 GEGPAIISNSTIPEN
G558 IPENVTSGGRFKLDV
S573 LKNKAKRSLTSSLEN
T575 NKAKRSLTSSLENIF
S576 KAKRSLTSSLENIFS
S577 AKRSLTSSLENIFSR
R584 SLENIFSRGANRMRG
S595 RMRGRLGSMDSFERA
S598 GRLGSMDSFERANSL
S604 DSFERANSLASEKDF
S607 ERANSLASEKDFSPG
S612 LASEKDFSPGDSPPG
S616 KDFSPGDSPPGTPPA
T620 PGDSPPGTPPASPLS
S624 PPGTPPASPLSSAWH
S627 TPPASPLSSAWHAFP
S628 PPASPLSSAWHAFPE
S640 FPEEDSDSPQFRRRA
T649 QFRRRAHTFSHPPSS
S651 RRRAHTFSHPPSSSR
S655 HTFSHPPSSSRRKLN
S656 TFSHPPSSSRRKLNL
S673-p GKAHGLRsPLLRQss
S679-p RsPLLRQsssEQCsD
S680-p sPLLRQsssEQCsDG
S681-p PLLRQsssEQCsDGE
S685-p QsssEQCsDGEGRKR
- gap
- gap
- gap
T693 DGEGRKRTSSTCSNE
S694 GEGRKRTSSTCSNES
S695 EGRKRTSSTCSNESL
T696 GRKRTSSTCSNESLN
S698 KRTSSTCSNESLNAG
S701 SSTCSNESLNAGGTP
N703 TCSNESLNAGGTPVT
T707 ESLNAGGTPVTPRRV
- gap
T710 NAGGTPVTPRRVSWR
S726 RIFLRVASPVNKSPS
S731 VASPVNKSPSAMQQQ
S733 SPVNKSPSAMQQQKD
S751 RTELLPLSPLSPTME
S754 LLPLSPLSPTMEEEP
- gap
- gap
- gap
- under review  
- gap
4288 : Phospho-AS160 (Thr642) Antibody
8881 : Phospho-AS160 (Thr642) (D27E6) Rabbit mAb
  rat

 
S66 MAEIRRRSLKPEAGG
G102 PAPGVGVGGGAGSGA
S230 DDCKDKFSLHEQQRL
S260-p MGVLEAEsPVsPDDG
S263-p LEAEsPVsPDDGLPE
D273 DGLPEKPDGLVNCPR
L275 LPEKPDGLVNCPRAL
C278 KPDGLVNCPRALPSL
S293 ASLPALASQPALASS
C312 PERILEDCGFDEQQE
S322 DEQQEFRSRCSSVTG
S325 QEFRSRCSSVTGVMQ
S326 EFRSRCSSVTGVMQK
T328 RSRCSSVTGVMQKKV
S350-p QPRRRHAsAPSHVQP
S353 RRHAsAPSHVQPSDS
S358 APSHVQPSDSEKNRT
S360 SHVQPSDSEKNRTML
T365 SDSEKNRTMLFQVGR
S379 RFEINLISPDTKSVV
K486 GLYPPRAKLVIQRHL
S494 LVIQRHLSSLTDNEQ
T537-p LCEAKQKtHVHIGEG
S549-p GEGQAIIsNSTIPEN
S559 TIPENSTSGGRFKLD
S575 LKNKAKRSLTssLEN
T577 NKAKRSLTssLENIF
S578-p KAKRSLTssLENIFS
S579-p AKRSLTssLENIFSR
R586 sLENIFSRGANRMRG
S597-p RMRGRLGsVDsFERS
S600-p GRLGsVDsFERSNsL
S606-p DsFERSNsLAsEKDF
S609-p ERSNsLAsEKDFsPG
S614-p LAsEKDFsPGGsPPG
S618-p KDFsPGGsPPGtPPA
T622-p PGGsPPGtPPAsPLS
S626-p PPGtPPAsPLSSAWH
S629 tPPAsPLSSAWHTFP
S630 PPAsPLSSAWHTFPE
S642 FPEEDSDSPQFRRRA
T651-p QFRRRAHtFSHPPSS
S653 RRRAHtFSHPPSSSR
S657 HtFSHPPSSSRRKLN
S658 tFSHPPSSSRRKLNL
S675 GKAHGLRSPLLRQNS
N681 RSPLLRQNSSEQCSV
S682 SPLLRQNSSEQCSVL
S683 PLLRQNSSEQCSVLP
S687 QNSSEQCSVLPSARR
T710 SSLPSLHTSFSAPSF
S713 PSLHTSFSAPSFTAS
S716 HTSFSAPSFTASSFL
T758 DGEGRKRTSSTCSNE
S759 GEGRKRTSSTCSNES
S760 EGRKRTSSTCSNESL
T761 GRKRTSSTCSNESLS
S763 KRTSSTCSNESLSAG
S766 SSTCSNESLSAGGTP
S768 TCSNESLSAGGTPVT
T772 ESLSAGGTPVTPRRI
- gap
T775 SAGGTPVTPRRISWR
S791 RIFLRVASPVNKSPS
S796 VASPVNKSPSAMQQQ
S798 SPVNKSPSAMQQQKD
S816-p RTELLPLsPLsPTME
S819-p LLPLsPLsPTMEEEP
S933 LKEGVPKSRRGEIWQ
S1137 VALSLLSSQEALVME
Y1202 DELLESSYACEDNES
N1218 EKLERANNQLKRQNM
A1257 TRETKMKALIRTLEQ
8619 : Phospho-AS160 (Ser318) (D3D11) Rabbit mAb
8730 : Phospho-AS160 (Ser588) (D8E4) Rabbit mAb
4288 : Phospho-AS160 (Thr642) Antibody
8881 : Phospho-AS160 (Thr642) (D27E6) Rabbit mAb
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