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Protein Page:
ATR (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ATR a ser/thr kinase of the PIKK family most closely related to ATM. Activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]Q. Phosphorylates BRCA1, Chk1, MCM2, RAD17, RPA2, SMC1 and p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Kinase, protein; Kinase, lipid; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; Membrane protein, integral; ATYPICAL group; PIKK family; ATR subfamily
Cellular Component: nucleoplasm; XY body; PML body; chromosome
Molecular Function: protein serine/threonine kinase activity; protein binding; DNA binding; MutLalpha complex binding; MutSalpha complex binding; ATP binding; protein kinase activity
Biological Process: response to drug; peptidyl-serine phosphorylation; negative regulation of DNA replication; protein amino acid autophosphorylation; multicellular organismal development; regulation of protein binding; DNA damage checkpoint; positive regulation of DNA damage response, signal transduction by p53 class mediator; DNA replication; cell cycle; DNA repair; response to DNA damage stimulus
Reference #:  Q13535 (UniProtKB)
Alt. Names/Synonyms: ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; FRAP-related protein 1; FRAP-related protein-1; FRP1; MEC1; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Rad3 related protein; SCKL; SCKL1; Serine/threonine-protein kinase ATR
Gene Symbols: ATR
Molecular weight: 301,367 Da
Basal Isoelectric point: 7.17  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint  |  Mitochondrial Control of Apoptosis
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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ATR

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell growth, altered: S428‑p
enzymatic activity, induced: S428‑p, T1989‑p
molecular association, regulation: T1989‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y26-p GSATPEEyNTVVQkP
0 1 K32-u EyNTVVQkPRQILCQ
1 0 Y291-p DTDQLKLyEEPLSKL
1 0 Y310-p FPFEAEAyRNIEPVy
0 1 Y317-p yRNIEPVyLNMLLEK
8 4 S428-p SSNSDGIsPKRRRLs
0 80 S435-p sPKRRRLsssLNPsk
0 47 S436-p PKRRRLsssLNPskR
0 5 S437-p KRRRLsssLNPskRA
0 2 S441-p LsssLNPskRAPKQT
0 1 K442-u sssLNPskRAPKQTE
0 1 K866-u ISRNNELkDTLILTT
0 2 K1005-u LLPDLAAkASPAASA
1 0 T1566-p DDQHTINtQDIASDL
1 0 T1578-p SDLCQLStQTVFSML
1 0 T1589-p FSMLDHLtQWARHKF
0 1 K1613-u HSKSNRNkVDSMVST
0 4 S1645-p QDTLAVAsFRSKAYT
0 1 K1703-u RKAEPSLkEQILEHE
0 2 K1824-u TAFYDSLkLVRAEQI
0 1 S1871-p IKPLFQHsPGDSsQE
0 1 S1876-p QHsPGDSsQEDSLNW
3 29 T1989-p LCFPENEtPPEGKNM
0 1 T2219 VGDATRLTDKLLELC
0 1 K2587-u TGEVVNEkAKTHVLD
0 1 K2604-u QRLQGVIkTRNRVTG
2853 : Phospho-ATR (Ser428) Antibody
  mouse

 
Y26 GSATPEEYNTVVQKP
K32 EYNTVVQKPRQILCQ
Y294 DADQLKLYEEPLSKL
Y313 FPFEAEAYRNIEPVY
Y320 YRNIEPVYLNVLLEK
S431-p SGNNDEVsPKRRKLs
S438-p sPKRRKLsSSLSSYK
S439 PKRRKLsSSLSSYKK
S440 KRRKLsSSLSSYKKP
Y444 LsSSLSSYKKPSRQP
K445 sSSLSSYKKPSRQPE
K866 IARNNELKDTLILTT
K1005 LLPDLAAKASPAASA
T1566 DEQHAISTQDSASDL
T1578 SDLCQLSTQTVFSVL
T1589 FSVLDHLTQWARHKF
- gap
S1639 QDTLAVASFRSKAYT
K1697 RKAEPSLKEQILEHE
K1818 TTFYDTLKLVRAEQI
S1865 LKPLFRKSPGDSCNE
C1870 RKSPGDSCNEDSLNW
S1983 LCFPENKSPSESKHM
T2213-p VGDATRLtDKLLELC
K2578 TGEVVNEKAKTHVLD
K2595 QRLQGVIKTRNRVTG
2853 : Phospho-ATR (Ser428) Antibody
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